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bacillus
Very Serious Mushronomer


Registered: 12/07/17
Posts: 394
Loc: Central Europe
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New horizons in sterile technique: SAC (Still Air Carton) 1
#24915424 - 01/15/18 10:31 AM (7 years, 2 days ago) |
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When I contemplated sterile work, I first learned about gloveboxes. Then about laminar flow hoods. Then, thanks to the Bod's wonderful post, about still air boxes. Now I'm attempting to take it even further with a SAC (still air carton).

I took a carton and put in on its side. It's 40cm high, 60cm wide and 41cm deep. I sprayed it with 0.03% hydrogen peroxide. Then I attempted an A2A transfer as I normally would, with one difference: I wore a surgeon's mask.

I transfered four wedges from one dish to two fresh double-chambered dishes.

D+5 update
D+14 update

Votes accepted from 01/15/18 09:27 AM until 01/18/18 09:27 AM
Edited by bacillus (01/29/18 12:18 PM)
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Mahdi
Temporal



Registered: 12/17/16
Posts: 338
Last seen: 10 days, 16 hours
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Re: New horizons in sterile technique: SAC (Still Air Carton) [Re: bacillus]
#24915438 - 01/15/18 10:40 AM (7 years, 2 days ago) |
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Its probably easier to work with your materials than in an SAB, it would greatly depend on flow and cleanliness of the air youre working around. Keep us updated how well it works!
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TheMilkMan84
Reincarnation of Benjamin Breeg


Registered: 03/01/17
Posts: 756
Loc: Caught somewhere in time
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Re: New horizons in sterile technique: SAC (Still Air Carton) [Re: bacillus]
#24915573 - 01/15/18 11:57 AM (7 years, 2 days ago) |
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Wouldn’t your contamination rate increase due to working under organic material?
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lewp
Seeker



Registered: 06/04/17
Posts: 403
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Re: New horizons in sterile technique: SAC (Still Air Carton) [Re: Mahdi]
#24915639 - 01/15/18 12:24 PM (7 years, 2 days ago) |
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I'd imagine your results are comparable to open air work.
-------------------- Wassup Fresh? It's our turn baby
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OfTheVoid46
Timeless



Registered: 07/10/16
Posts: 148
Loc: USA
Last seen: 5 years, 10 months
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Re: New horizons in sterile technique: SAC (Still Air Carton) [Re: bacillus]
#24915918 - 01/15/18 02:22 PM (7 years, 2 days ago) |
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Before I had a proper SAB, I had a box like that. Rather than using it like you I cut off the top flaps and taped saran wrap over it in a few layers so it was sealed and cut arm holes big enough to also put jars through. I also lined the inside with plastic and taped it all.
When it came time to use, I'd spray the inside with lysol, wipe it down, wipe down the items as I'd put them in and then tape pieces of plastic over the arm holes while the air died down. A couple hours later I'd go back nice and clean with sleeves and gloves and did my thing after taking off the plastic covering the holes. I never had anything contaminate including WBS and Agar after using that box for nearly a year.
If your budget is low you can turn that into something a little better with a little saran wrap or other plastic, cheap tape and some disinfectant spray.
To answer your question about contams: that depends on how clean you are yourself and the room you're in. Your box might be blocking a little bit of particles but not much. Work slowly, as in slow movements as to not stir up air too much.
Chances are you'll pull it off. I gave ya 50%
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Edited by OfTheVoid46 (01/15/18 02:28 PM)
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Mr. Bojangles
Breathe In



Registered: 04/08/08
Posts: 1,938
Loc: The Dirty
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Re: New horizons in sterile technique: SAC (Still Air Carton) [Re: OfTheVoid46]
#24916014 - 01/15/18 03:06 PM (7 years, 2 days ago) |
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Cardboard is generally frowned upon in aseptic environments. Its dirty to start with and harbors contaminants/bugs/microorganisms over time.
-------------------- "It is dangerous to be right in matters on which the established authorities are wrong."
Francois-Marie Arouet
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Alan Rockefeller
Mycologist

Registered: 03/10/07
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Re: New horizons in sterile technique: SAC (Still Air Carton) [Re: Mr. Bojangles]
#24917379 - 01/16/18 01:46 AM (7 years, 2 days ago) |
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Since its just cardboard you might as well spray down with 10% bleach, I think it will be more effective than H2O2.
It might be a good idea to use something clear for the sides, maybe even add some glass. Cardboard is hard to see through.
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bacillus
Very Serious Mushronomer


Registered: 12/07/17
Posts: 394
Loc: Central Europe
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Re: New horizons in sterile technique: SAC (Still Air Carton) [Re: Alan Rockefeller]
#24917562 - 01/16/18 06:10 AM (7 years, 1 day ago) |
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Guys, thank you for the feedback. You are great.
Now, I would like to emphasize that SAC is an experiment. Normally I use a fish tank with one side covered and it works quite well. It's very transparent. For this I chose carton precisely because it's so imperfect. And it could still work, conceivably, because it provides a roof and some walls.
Not long ago I believed sterile work to be very difficult in home conditions. You know, leave a flow hood running in a clean room, take a shower, shave your armpits, and so on.
Then came the shock of learning that the second-best solution is a plastic box with two big holes. Without disinfectant, even. Although I still use isopropanol. I guess I like the smell.
Anyway, I'm updating the post with the dish pictures.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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Re: New horizons in sterile technique: SAC (Still Air Carton) (moved) [Re: bacillus]
#24918001 - 01/16/18 10:50 AM (7 years, 1 day ago) |
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This thread was moved from Advanced Mycology.
Reason: Better fit.
People have made cardboard SABs before. But i would avoid cardboard if at all possible
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bacillus
Very Serious Mushronomer


Registered: 12/07/17
Posts: 394
Loc: Central Europe
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Re: New horizons in sterile technique: SAC (Still Air Carton) (moved) [Re: bodhisatta] 2
#24927396 - 01/20/18 07:01 AM (6 years, 11 months ago) |
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Updated the post with pictures five days after. No apparent infections.
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