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more4u2c
Mad Hatter

Registered: 07/08/14
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Are spores 100% new genetics or do they carry over from the fruit that shit them out?
#24912545 - 01/14/18 01:41 AM (7 years, 4 days ago) |
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So anyone with actual knowledge know if the spores from a fruit are 100% new genetics or do they carry genes from their fruit they came from?
Like I have a spore print from a old ass grow I don't even know the "strains" name but ive grown a MS grow from it and it produces mostly small weak fruits. Does this mean that this spore print is gonna always grow like this and I should make a new spore print from a big fruit that does pop up or do spores not bring any genetic info with them and the same spore print can eventually make a knockout fruiter?
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,619
Loc: Michigan
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Re: Are spores 100% new genetics or do they carry over from the fruit that shit them out? [Re: more4u2c] 1
#24912577 - 01/14/18 02:00 AM (7 years, 4 days ago) |
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It has a higher chance of creating those same looking fruits.
You can still get 1000s of different mushrooms. It takes a long time to stabilize that kinda stuff. So if you find a big on print it and continue printing big ones eventually you'll have higher chances of getting big fruits
So they carry over yes.
That make sense?
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more4u2c
Mad Hatter

Registered: 07/08/14
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Re: Are spores 100% new genetics or do they carry over from the fruit that shit them out? [Re: van hatton] 1
#24912626 - 01/14/18 03:01 AM (7 years, 4 days ago) |
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Yes makes sense I e been doing some reading on the interwebs after I posted this post and now have a follow up question maybe I should move this to advance forum but let's see how it does in here lol
Follow up question. In my very limited study of this subject what is it called and is it better to do the following
I have grown out myc on agar from old spore print been trying to transfer to an isolate but still getting sectors but let's say once I get 4 different isolates from this old spore agar work and I fruit them out and let's say 2 out of the 4 produce big fruits I get a spore print from both big fruiting isolates I then grab a agar dish and mix a tiny amount of each spore print onto the agar thus making the 2 different isolated fruit spores mix together. Would this up my percentage of getting more isolated big fruiter? Is this considered creating a new "strain"
From my limited reading on this subject it seems that it's better to mix spores from 2 different fruits than to mix spores from the same fruit. Like mixing spores from the same fruit is like making the spores fuck their brothers and sisters vs mixing them with a different fruits spores is akin to spore one night stands at the local pub.
It's hard to get spores in the area I'm in so I'm kinda stuck with this one old spore print and whatever I can grow from it and I really wanna learn how to work this into a nice strong heavy fruiter just tryn to learn the best possible way to do this I assume it's gonna take a shit ton of agar work and spores and isolates to eventually get there any help or suggestions are welcomed and Appreciated
Edited by more4u2c (01/14/18 03:06 AM)
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LogicaL Chaos
Ascension Energy & Alien UFOs




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Re: Are spores 100% new genetics or do they carry over from the fruit that shit them out? [Re: more4u2c]
#24912654 - 01/14/18 03:43 AM (7 years, 4 days ago) |
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"fruit that shit them out".

Spore prints hold tons of genetic material. Some of its from the fruit, most is not. Most is random cubenis strain DNA related to that strain.
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nube424

Registered: 12/03/17
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Re: Are spores 100% new genetics or do they carry over from the fruit that shit them out? [Re: more4u2c]
#24912766 - 01/14/18 06:26 AM (7 years, 3 days ago) |
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Why dont u clone instead of spore to agar? When u get a big fruit and a cluster, clone it. Pretty sure ull get what u want way faster than trying to isolate from spores
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mushpunx
Fungus Punk



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Re: Are spores 100% new genetics or do they carry over from the fruit that shit them out? [Re: nube424]
#24912805 - 01/14/18 07:07 AM (7 years, 3 days ago) |
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Don't just keep transferring it until it doesn't sector anymore. You could do that, isolate one strain at a time from the print, but its really much better to aim for 10-20 isolates from the one culture.
Transfer until you see really prominent sectoring- could take as many as 10 transfers.
Then take a transfer from each sector to a new plate- if you are lucky they will be isolates but I usually have to make another transfer or two before they stop sectoring.
Then label and store a sample of each of the 10-20 isolates and test them out. I usually only find one or two keepers
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more4u2c
Mad Hatter

Registered: 07/08/14
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Re: Are spores 100% new genetics or do they carry over from the fruit that shit them out? [Re: nube424]
#24913570 - 01/14/18 01:36 PM (7 years, 3 days ago) |
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Quote:
nube424 said: Why dont u clone instead of spore to agar? When u get a big fruit and a cluster, clone it. Pretty sure ull get what u want way faster than trying to isolate from spores
I've tried cloning big fruits and it still grows out smaller fruits no where near what the cloned fruit looked like when sample was taken to agar.
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nube424

Registered: 12/03/17
Posts: 6,063
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Re: Are spores 100% new genetics or do they carry over from the fruit that shit them out? [Re: more4u2c]
#24913580 - 01/14/18 01:41 PM (7 years, 3 days ago) |
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Sounds like a moisture/humidity problem. Could be low humidity in ur setup or low substrate water
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more4u2c
Mad Hatter

Registered: 07/08/14
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Re: Are spores 100% new genetics or do they carry over from the fruit that shit them out? [Re: mushpunx]
#24913584 - 01/14/18 01:42 PM (7 years, 3 days ago) |
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Quote:
mushpunx said: Don't just keep transferring it until it doesn't sector anymore. You could do that, isolate one strain at a time from the print, but its really much better to aim for 10-20 isolates from the one culture.
Transfer until you see really prominent sectoring- could take as many as 10 transfers.
Then take a transfer from each sector to a new plate- if you are lucky they will be isolates but I usually have to make another transfer or two before they stop sectoring.
Then label and store a sample of each of the 10-20 isolates and test them out. I usually only find one or two keepers
That's what I'm doing sorta like I started with a MS agar grew it out and got 6 sectors and transferred them to agar then from those 6 dishes I transferred like 9 new sectors (choosing the fastest and most rizo growing myc) I'm now waiting for those 9 to grow out. My theory is eventually I'll get 15 or 20 isolates all fast growing rizo myc. But my one issue is im totally running out of jars doing it this way lol
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mushpunx
Fungus Punk



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Re: Are spores 100% new genetics or do they carry over from the fruit that shit them out? [Re: more4u2c]
#24913868 - 01/14/18 03:38 PM (7 years, 3 days ago) |
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How many transfers did you make before you started seeing sectoring? When you transfer from a sector you should have an isolate. Ocasionaly you might see sectoring on the transfer because you grabbed from 2 or 3 sectors that were in the same fan. If you make more transfers away from germination it becomes easier to pick individual sectors
Yea man strain isolation eats plates! You'll be swimming in cultures by the time you're done haha Its why I mostly clone I'm excited to see what you come up with!!
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more4u2c
Mad Hatter

Registered: 07/08/14
Posts: 526
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Re: Are spores 100% new genetics or do they carry over from the fruit that shit them out? [Re: more4u2c]
#24914418 - 01/14/18 07:03 PM (7 years, 3 days ago) |
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So here some pictures With no light
 With light on

Now there is no sectoring but it's not growing out evenly so I'm under the impression that this isn't yet an isolate cuz they are suppose to grow out perfectly round. Is this right?
Here are some other agar dishes these are I believe 4th or 5th transfer from spore I sorta lost count lol fml SMH as you can see they are not growing out evenly so they obviously still have more transfers to go before being a mono strain isolate correct?
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more4u2c
Mad Hatter

Registered: 07/08/14
Posts: 526
Last seen: 4 months, 4 days
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Re: Are spores 100% new genetics or do they carry over from the fruit that shit them out? [Re: more4u2c]
#24914422 - 01/14/18 07:04 PM (7 years, 3 days ago) |
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And I saw sectoring after first transfer from MS
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more4u2c
Mad Hatter

Registered: 07/08/14
Posts: 526
Last seen: 4 months, 4 days
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Re: Are spores 100% new genetics or do they carry over from the fruit that shit them out? [Re: nube424]
#24914445 - 01/14/18 07:19 PM (7 years, 3 days ago) |
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Quote:
nube424 said: Sounds like a moisture/humidity problem. Could be low humidity in ur setup or low substrate water
Yeah to be honest I could be having a bunch of problems lol this is why I'm trying to cover all my bases for my next attempt. I was fruiting in mini mono tubs and I'm thinking that might have also been a reason for smaller fruits so this next grow I'm going back to bigger tubs. Also I was doing 1 to 1 ratio with my spawn and I was told I might wanna do more bulk to spawn ratio to give the substrate more moisture bulk thus why I'm tryn so hard to find a fast spreading myc iso so when I go 3 to 1 or 4 to 1 it can run through all that bulk and colonize before contams try to get in. I'm also going back to my roots and just going to go with coir/verm to also limit any contams risk
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more4u2c
Mad Hatter

Registered: 07/08/14
Posts: 526
Last seen: 4 months, 4 days
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Re: Are spores 100% new genetics or do they carry over from the fruit that shit them out? [Re: more4u2c]
#24914450 - 01/14/18 07:21 PM (7 years, 3 days ago) |
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Can over misting cause them to grow small? Cuz I first would mist up to pins then stop then I read on here that was outdated info and that it's ok to mist all the way up to harvest so I would mist everyday. Maybe I made them too wet? I was told there is no such thing as too wet when it comes to fruiting
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