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Offlinerifters360
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FIRE IN SAB! info...
    #24909104 - 01/12/18 09:31 PM (7 years, 5 days ago)

Ok so i a complete noob. I have tried to read over the guidelines so if this post gets moved or bumped i get it thanks. I am moving into agar for the first time. I read as much as i could and everything was going prettynwell for a noob with an SAB until i got to the inoculation. I sanitized best i could with alcohol and a disinfectant spray. And did to well with the alcohol. The fumes from my gloves and tyvek sleeves ignited. And then a paper towel with 2 drops ignited inside the SAB... Well i commenced to ROFLCOPTR as i thought it was hilarious. Actually i flung a sterile syringe into my couch and stopped,dropped and rolled and wasnt burned at all luckily. I was still laughing at my stupidity. But my question is that would the smoke in the box, which was very minuscule
actually, as it was all alcohol mostly. And the fire was put out instantly, would it affect the agar that i inoculated after, and the catch that may save me is that i sealed all my agar in sandwich baggies before the accident, and im using plastic dishes. Luckily my best agar sample was done right before and only needed to make two more. How should i approach this situation? And for next time i think i will try  to use peroxide or bleach for sanitizing. So basically is the agar fucked from the smoke and flash of heat even though they were in sealed plastic bags?


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Let It Be...

Edited by rifters360 (01/12/18 10:35 PM)

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InvisibleSubnet Mask
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Re: FIRE IN SAB! info... [Re: rifters360]
    #24909110 - 01/12/18 09:34 PM (7 years, 5 days ago)

:congrats:

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InvisibleNorsern_vind
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Registered: 08/22/17
Posts: 214
Re: FIRE IN SAB! info... [Re: Subnet Mask] * 1
    #24909117 - 01/12/18 09:36 PM (7 years, 5 days ago)

I have no idea why you are even using a flame inside the SAB.

Please reference Bod's SAB tek so you don't kill yourself.

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OfflineEverett P
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Registered: 10/18/17
Posts: 133
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Re: FIRE IN SAB! info... [Re: rifters360]
    #24909121 - 01/12/18 09:38 PM (7 years, 5 days ago)

U should be good next time ditch the sleeves and switch to 70% iso  just slather your arms in it 70% is less likely to ignite also u can flame ur needle outside of the sab then cover with iso soaked papertowel until inside the box


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interested in prints related to treating auto immune such as RA  and lupus thank you pm me have O.I. Prints to trade

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Invisibleyoosername
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Re: FIRE IN SAB! info... [Re: Norsern_vind]
    #24909144 - 01/12/18 09:54 PM (7 years, 5 days ago)

You aren't a true mycologist until you've set yourself on fire at least once, congratulations!  Agar should be fine, prepare more if you're worried about it, and stop using flammable liquids in proximity to torches.
Alcohol, bleach, etc. will not completely sterilize anyways, you can get by with soapy water and good technique.  Flame needle/tool outside SAB, cool inside by ejecting liquid or dipping in agar.  Be mindful of gravity and your fleshflakes, you dirty opisthokont :wink:


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O son of Kunti, I am the taste of water, the light of the sun and the moon, the syllable om in the Vedic mantras; I am the sound in ether and ability in man.

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OfflineThreads from God89
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Re: FIRE IN SAB! info... [Re: Norsern_vind]
    #24909148 - 01/12/18 09:55 PM (7 years, 5 days ago)

Quote:

Norsern_vind said:
I have no idea why you are even using a flame inside the SAB.

Please reference Bod's SAB tek so you don't kill yourself.




Holy shit man!!! Oh my God!...LMFAO!!!  I visualized this cat dropping and rollin' with burning paper towel embers cascading down and shit!!  O wow man, damn, got a good laugh outta that! :lol:

  Yeah don't use flammable compounds and fire in the same space please!!!  And to think they gave me shit for my oven door technique! (Don't use that one either!).

    Yeah, check out that Tek that was referenced above!  It utilizes soap and water spray or something I think...?  Not positive tho...:thumbup:

          Glad nobody got hurt!!! :rockon:


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Admirer of Bushido....... Seeker of Truth, and Knowledge.

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OfflinecronicrMFacebook
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Re: FIRE IN SAB! info... [Re: Everett P] * 1
    #24909152 - 01/12/18 09:56 PM (7 years, 5 days ago)

It is called a still air box...no need to spray alcohol my man! Soap n water is all ya need


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It doesn't matter what i think of you...all that matters is clean spawn
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Offlinerifters360
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Re: FIRE IN SAB! info... [Re: cronicr]
    #24909217 - 01/12/18 10:29 PM (7 years, 5 days ago)

I swear the tek instructed one flame sterilize in the box. I thought i would blow up a pressure cooker before this haha but u live and u learn. I will let yall know how it goes:confused:


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Let It Be...

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Offlinemushpunx
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Re: FIRE IN SAB! info... [Re: rifters360]
    #24909252 - 01/12/18 10:39 PM (7 years, 5 days ago)

This actually happens a lot more than you would think, new cultivator wipes the walls down with ISO, fumes build, they put thier arms in and try to flame something with a Bic lighter and BOOM!
Hahaha.

Before I started using a propane torch, I had an alcohol lamp. I'd wash my gloves with ISO before I'd start and go to light my lamp (or it'd be lit and I'd hold a tool above it) , set my gloves on fire :facepalm:

We live we learn haha


--------------------

Amateur Mycologists United
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InvisibleZiran
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Re: FIRE IN SAB! info... [Re: cronicr]
    #24909275 - 01/12/18 10:52 PM (7 years, 5 days ago)

Quote:

cronicr said:
It is called a still air box...no need to spray alcohol my man! Soap n water is all ya need




Yep Yep.


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Song Of Healing
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The Chinese word for nature is zìrán and it means that of which is of itself.


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OfflineSpiritwalker_Faust
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Re: FIRE IN SAB! info... [Re: Ziran] * 1
    #24909361 - 01/12/18 11:31 PM (7 years, 5 days ago)

Did that with a Lysol overload once, blew the lid off my sab, hands and arms baby smooth. Now I don't use lysol

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OfflineMarmie
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Re: FIRE IN SAB! info... [Re: Spiritwalker_Faust]
    #24909471 - 01/13/18 12:17 AM (7 years, 4 days ago)

Atleast you know everything got good n steralized :lol:


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OfflineUnsungHero
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Re: FIRE IN SAB! info... [Re: Marmie] * 2
    #24910868 - 01/13/18 12:56 PM (7 years, 4 days ago)

One must always practice common sense, even if the tek says otherwise.


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"The quieter you become, the more you are able to hear."

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OfflineCybin_man
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Re: FIRE IN SAB! info... [Re: rifters360]
    #24910953 - 01/13/18 01:33 PM (7 years, 4 days ago)

https://www.shroomery.org/forums/showflat.php/Number/24312004#24312004
Some good videos to watch. Especially the SAB videos


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mushrooms + my morning jacket = awesome https://youtu.be/xkY4isMi2Zc


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InvisibleApples in Mono
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Registered: 09/21/17
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Re: FIRE IN SAB! info... [Re: Everett P]
    #24910959 - 01/13/18 01:35 PM (7 years, 4 days ago)

Quote:

Everett P said:
U should be good next time ditch the sleeves and switch to 70% iso  just slather your arms in it 70% is less likely to ignite also u can flame ur needle outside of the sab then cover with iso soaked papertowel until inside the box




Don't wipe the needle with alcohol(or anything else) after flame sterilization. Alcohol doesn't sterilize; it sanitizes- big difference

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InvisibleTheMadHatter420
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Re: FIRE IN SAB! info... [Re: Everett P]
    #24911123 - 01/13/18 02:55 PM (7 years, 4 days ago)

Quote:

Everett P said:
cover with iso soaked papertowel until inside the box




This is BAD info to push. Doing so defeats the purpose of flame sterilizations as you just put an unsterile paper towel over it. Alcohol does NOT sterilize!


--------------------
JOIN THE POW WOW

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Offlineshade12435
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Re: FIRE IN SAB! info... [Re: TheMadHatter420]
    #24911153 - 01/13/18 03:11 PM (7 years, 4 days ago)

I wipe down everything in my still air box with 70% iso before each session. I do not, however, have any flame in there. Flame till red hot outside the box then bring it in to work....glad you didn't get burned badly.

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OfflineSadTurkey
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Re: FIRE IN SAB! info... [Re: shade12435]
    #24911167 - 01/13/18 03:18 PM (7 years, 4 days ago)

Spraying a SAB with any alcohol is pointless, dangerous and a waste of money.

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InvisiblePudinLicous
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Re: FIRE IN SAB! info... [Re: rifters360]
    #24911622 - 01/13/18 06:42 PM (7 years, 4 days ago)

Thanks for posting your hilarious story, rifters. That way at least other people can learn. I laughed my ass off. I remember as a noob having my alcohol lamp in my SAB. Luckily I never burst into flames, but looking back it was a miracle. I did learn the hard way however that blunt tip syringes needles are indeed sharp as fuck and biopsied my hand accidentally. :thumbup:

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Offlinerifters360
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Re: FIRE IN SAB! info... [Re: PudinLicous]
    #24915340 - 01/15/18 09:40 AM (7 years, 2 days ago)

yep well I need to make my first few posts to access everything so heres a start. so thats how much of a noob i am


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Let It Be...

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InvisibleCitizen X
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Re: FIRE IN SAB! info... [Re: Spiritwalker_Faust]
    #24915365 - 01/15/18 09:53 AM (7 years, 2 days ago)

I had my alcohol (homemade) tip over and liquid flame went everywhere. I was scared shitless and almost burned my house down.

These days I am just super careful with with fire when I’m doing my hobby. Lesson learned brother and you sure aren’t alone :rockon:
Quote:

Spiritwalker_Faust said:
Did that with a Lysol overload once, blew the lid off my sab, hands and arms baby smooth. Now I don't use lysol


:rofl2:


--------------------


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Offlinekrypto2000
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Re: FIRE IN SAB! info... [Re: Everett P]
    #24915439 - 01/15/18 10:40 AM (7 years, 2 days ago)

Quote:

Everett P said:
U should be good next time ditch the sleeves and switch to 70% iso  just slather your arms in it 70% is less likely to ignite also u can flame ur needle outside of the sab then cover with iso soaked papertowel until inside the box




If you sterilize your needle and then wipe it with an iso soaked paper towel you're just contaminating it again, alcohol is not sterile and it does not efficetively sterilize, bacterial spores can survive in alcohol.

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OfflineHugh Jorgan
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Re: FIRE IN SAB! info... [Re: krypto2000]
    #24915536 - 01/15/18 11:39 AM (7 years, 2 days ago)

Oh My God..... I'm glad you're OK. m a former firefighter and I cant stop laughing. I know its wrong but just visualizing it has me laughing my ass off.  I'm surprised that flames didn't shoot out of the armholes in the SAB. Alcohol evaporates fast and filling up an SAB with those fumes and then igniting it must be rater exciting.

Just be glad you have the opportunity to learn from your mistakes. NO FIRE in the SAB!
Good luck, be careful and have fun growin!!


--------------------
Always go forward! Never go straight!!



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Offlinerifters360
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Re: FIRE IN SAB! info... [Re: Hugh Jorgan]
    #24933086 - 01/22/18 06:38 AM (6 years, 11 months ago)

So the agar is doing well think. I had 1 contam out of 4 so far. and then i did 2 transfers from all of the healthiest areas i could see. Im not going for a monoculture specifically but jus limited genetics. And Im trying to figure out how to upload pics properly.


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Let It Be...

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Offlinerifters360
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Re: FIRE IN SAB! info... [Re: rifters360]
    #24933088 - 01/22/18 06:39 AM (6 years, 11 months ago)

they did shoot out buy the way in quick whoosh!


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Let It Be...

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