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rifters360
Stranger



Registered: 11/04/17
Posts: 35
Loc: The Bible Belt South
Last seen: 2 years, 2 months
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FIRE IN SAB! info...
#24909104 - 01/12/18 09:31 PM (7 years, 5 days ago) |
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Ok so i a complete noob. I have tried to read over the guidelines so if this post gets moved or bumped i get it thanks. I am moving into agar for the first time. I read as much as i could and everything was going prettynwell for a noob with an SAB until i got to the inoculation. I sanitized best i could with alcohol and a disinfectant spray. And did to well with the alcohol. The fumes from my gloves and tyvek sleeves ignited. And then a paper towel with 2 drops ignited inside the SAB... Well i commenced to ROFLCOPTR as i thought it was hilarious. Actually i flung a sterile syringe into my couch and stopped,dropped and rolled and wasnt burned at all luckily. I was still laughing at my stupidity. But my question is that would the smoke in the box, which was very minuscule actually, as it was all alcohol mostly. And the fire was put out instantly, would it affect the agar that i inoculated after, and the catch that may save me is that i sealed all my agar in sandwich baggies before the accident, and im using plastic dishes. Luckily my best agar sample was done right before and only needed to make two more. How should i approach this situation? And for next time i think i will try to use peroxide or bleach for sanitizing. So basically is the agar fucked from the smoke and flash of heat even though they were in sealed plastic bags?
-------------------- Let It Be...
Edited by rifters360 (01/12/18 10:35 PM)
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Subnet Mask


Registered: 05/08/15
Posts: 903
Loc:
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Norsern_vind
Alchemical Wizard


Registered: 08/22/17
Posts: 214
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I have no idea why you are even using a flame inside the SAB.
Please reference Bod's SAB tek so you don't kill yourself.
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Everett P
SeeR



Registered: 10/18/17
Posts: 133
Loc: Sewanee
Last seen: 6 years, 3 months
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U should be good next time ditch the sleeves and switch to 70% iso just slather your arms in it 70% is less likely to ignite also u can flame ur needle outside of the sab then cover with iso soaked papertowel until inside the box
-------------------- interested in prints related to treating auto immune such as RA and lupus thank you pm me have O.I. Prints to trade
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yoosername
Lab Member



Registered: 03/28/13
Posts: 1,076
Loc: Terra Fracta
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You aren't a true mycologist until you've set yourself on fire at least once, congratulations! Agar should be fine, prepare more if you're worried about it, and stop using flammable liquids in proximity to torches. Alcohol, bleach, etc. will not completely sterilize anyways, you can get by with soapy water and good technique. Flame needle/tool outside SAB, cool inside by ejecting liquid or dipping in agar. Be mindful of gravity and your fleshflakes, you dirty opisthokont
-------------------- O son of Kunti, I am the taste of water, the light of the sun and the moon, the syllable om in the Vedic mantras; I am the sound in ether and ability in man.
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Threads from God89
Pioneer.......


Registered: 08/10/17
Posts: 1,916
Loc: USA
Last seen: 17 days, 14 hours
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Quote:
Norsern_vind said: I have no idea why you are even using a flame inside the SAB.
Please reference Bod's SAB tek so you don't kill yourself.
Holy shit man!!! Oh my God!...LMFAO!!! I visualized this cat dropping and rollin' with burning paper towel embers cascading down and shit!! O wow man, damn, got a good laugh outta that! 
Yeah don't use flammable compounds and fire in the same space please!!! And to think they gave me shit for my oven door technique! (Don't use that one either!).
Yeah, check out that Tek that was referenced above! It utilizes soap and water spray or something I think...? Not positive tho...
Glad nobody got hurt!!!
-------------------- Admirer of Bushido....... Seeker of Truth, and Knowledge.
I Will Keep the Fire going, and the Good Wolf fed.......
( We Are All Threads from God.)
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cronicr



Registered: 08/07/11
Posts: 62,899
Loc: Van Isle
Last seen: 42 minutes, 31 seconds
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Re: FIRE IN SAB! info... [Re: Everett P] 1
#24909152 - 01/12/18 09:56 PM (7 years, 5 days ago) |
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It is called a still air box...no need to spray alcohol my man! Soap n water is all ya need
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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rifters360
Stranger



Registered: 11/04/17
Posts: 35
Loc: The Bible Belt South
Last seen: 2 years, 2 months
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Re: FIRE IN SAB! info... [Re: cronicr]
#24909217 - 01/12/18 10:29 PM (7 years, 5 days ago) |
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I swear the tek instructed one flame sterilize in the box. I thought i would blow up a pressure cooker before this haha but u live and u learn. I will let yall know how it goes
-------------------- Let It Be...
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 2 months, 7 days
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This actually happens a lot more than you would think, new cultivator wipes the walls down with ISO, fumes build, they put thier arms in and try to flame something with a Bic lighter and BOOM! Hahaha.
Before I started using a propane torch, I had an alcohol lamp. I'd wash my gloves with ISO before I'd start and go to light my lamp (or it'd be lit and I'd hold a tool above it) , set my gloves on fire
We live we learn haha
--------------------

Amateur Mycologists United
AMU Q&A
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Ziran
The Hero of Time




Registered: 02/03/16
Posts: 6,034
Loc: Temple of Time
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Re: FIRE IN SAB! info... [Re: cronicr]
#24909275 - 01/12/18 10:52 PM (7 years, 5 days ago) |
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Quote:
cronicr said: It is called a still air box...no need to spray alcohol my man! Soap n water is all ya need
Yep Yep.
-------------------- Song Of Healing
Updated Pf Tek Guide
Ziran's Teks
AMU Q&A Thread
The Chinese word for nature is zìrán and it means that of which is of itself.

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Spiritwalker_Faust
Hominid



Registered: 02/13/14
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Re: FIRE IN SAB! info... [Re: Ziran] 1
#24909361 - 01/12/18 11:31 PM (7 years, 5 days ago) |
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Did that with a Lysol overload once, blew the lid off my sab, hands and arms baby smooth. Now I don't use lysol
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Marmie
Peter peter portion eater



Registered: 10/21/15
Posts: 926
Loc: Deep inside the night
Last seen: 5 days, 2 hours
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Atleast you know everything got good n steralized
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UnsungHero
Endure & Persist


Registered: 11/21/17
Posts: 495
Loc: root@remotehost
Last seen: 11 months, 16 days
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Re: FIRE IN SAB! info... [Re: Marmie] 2
#24910868 - 01/13/18 12:56 PM (7 years, 4 days ago) |
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One must always practice common sense, even if the tek says otherwise.
-------------------- "The quieter you become, the more you are able to hear."
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Cybin_man
Circle the Wagons


Registered: 05/02/17
Posts: 799
Loc: In the bathroom
Last seen: 4 years, 9 months
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-------------------- mushrooms + my morning jacket = awesome https://youtu.be/xkY4isMi2Zc
 
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Apples in Mono
Not a puppet


Registered: 09/21/17
Posts: 3,240
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Re: FIRE IN SAB! info... [Re: Everett P]
#24910959 - 01/13/18 01:35 PM (7 years, 4 days ago) |
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Quote:
Everett P said: U should be good next time ditch the sleeves and switch to 70% iso just slather your arms in it 70% is less likely to ignite also u can flame ur needle outside of the sab then cover with iso soaked papertowel until inside the box
Don't wipe the needle with alcohol(or anything else) after flame sterilization. Alcohol doesn't sterilize; it sanitizes- big difference
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,943
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Re: FIRE IN SAB! info... [Re: Everett P]
#24911123 - 01/13/18 02:55 PM (7 years, 4 days ago) |
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Quote:
Everett P said: cover with iso soaked papertowel until inside the box
This is BAD info to push. Doing so defeats the purpose of flame sterilizations as you just put an unsterile paper towel over it. Alcohol does NOT sterilize!
-------------------- JOIN THE POW WOW
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shade12435



Registered: 03/09/10
Posts: 69
Last seen: 2 years, 2 months
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I wipe down everything in my still air box with 70% iso before each session. I do not, however, have any flame in there. Flame till red hot outside the box then bring it in to work....glad you didn't get burned badly.
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SadTurkey
ayy


Registered: 10/21/17
Posts: 410
Loc: Netherlands
Last seen: 1 year, 4 months
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Spraying a SAB with any alcohol is pointless, dangerous and a waste of money.
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PudinLicous


Registered: 10/28/98
Posts: 152
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Thanks for posting your hilarious story, rifters. That way at least other people can learn. I laughed my ass off. I remember as a noob having my alcohol lamp in my SAB. Luckily I never burst into flames, but looking back it was a miracle. I did learn the hard way however that blunt tip syringes needles are indeed sharp as fuck and biopsied my hand accidentally.
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rifters360
Stranger



Registered: 11/04/17
Posts: 35
Loc: The Bible Belt South
Last seen: 2 years, 2 months
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yep well I need to make my first few posts to access everything so heres a start. so thats how much of a noob i am
-------------------- Let It Be...
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Citizen X
Buzz Killinton

Registered: 01/19/14
Posts: 7,890
Loc: Djibouti
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I had my alcohol (homemade) tip over and liquid flame went everywhere. I was scared shitless and almost burned my house down.
These days I am just super careful with with fire when I’m doing my hobby. Lesson learned brother and you sure aren’t alone  Quote:
Spiritwalker_Faust said: Did that with a Lysol overload once, blew the lid off my sab, hands and arms baby smooth. Now I don't use lysol
--------------------
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krypto2000
Unknown


Registered: 12/05/06
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Re: FIRE IN SAB! info... [Re: Everett P]
#24915439 - 01/15/18 10:40 AM (7 years, 2 days ago) |
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Quote:
Everett P said: U should be good next time ditch the sleeves and switch to 70% iso just slather your arms in it 70% is less likely to ignite also u can flame ur needle outside of the sab then cover with iso soaked papertowel until inside the box
If you sterilize your needle and then wipe it with an iso soaked paper towel you're just contaminating it again, alcohol is not sterile and it does not efficetively sterilize, bacterial spores can survive in alcohol.
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Hugh Jorgan
Mycophile



Registered: 09/21/15
Posts: 173
Last seen: 3 years, 9 months
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Oh My God..... I'm glad you're OK. m a former firefighter and I cant stop laughing. I know its wrong but just visualizing it has me laughing my ass off. I'm surprised that flames didn't shoot out of the armholes in the SAB. Alcohol evaporates fast and filling up an SAB with those fumes and then igniting it must be rater exciting.
Just be glad you have the opportunity to learn from your mistakes. NO FIRE in the SAB! Good luck, be careful and have fun growin!!
-------------------- Always go forward! Never go straight!!
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rifters360
Stranger



Registered: 11/04/17
Posts: 35
Loc: The Bible Belt South
Last seen: 2 years, 2 months
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So the agar is doing well think. I had 1 contam out of 4 so far. and then i did 2 transfers from all of the healthiest areas i could see. Im not going for a monoculture specifically but jus limited genetics. And Im trying to figure out how to upload pics properly.
-------------------- Let It Be...
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rifters360
Stranger



Registered: 11/04/17
Posts: 35
Loc: The Bible Belt South
Last seen: 2 years, 2 months
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Re: FIRE IN SAB! info... [Re: rifters360]
#24933088 - 01/22/18 06:39 AM (6 years, 11 months ago) |
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they did shoot out buy the way in quick whoosh!
-------------------- Let It Be...
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