Home | Community | Message Board


World Seed Supply
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Gourmet and Medicinal Mushrooms

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: MushCult - Live Mushroom Cultures Edible and Medicinal Mushroom Cultures on Agar   Amazon ½ Pint Jars, Agar, Autoclave, Parafilm, Petri Dish

Jump to first unread post. Pages: 1
Offlinetscott1632
Shroomery Creeper
 User Gallery

Registered: 10/14/13
Posts: 243
Loc: Montana
Last seen: 7 days, 16 hours
Sending wedges in Micro Centrifuge tube
    #24908451 - 01/12/18 07:05 PM (9 months, 1 day ago)

I want to get into the VVVVVV wedge tread. I was searching on the forum and found that a lot of people like to send theirs in a micro centrifuge. My question is what size do you guys like and are all centrifuge tubes pc'able or will some melt.
Thanks for the help


--------------------
Trade List


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinetiedma
Fat Kid
Male

Registered: 08/16/17
Posts: 119
Last seen: 4 days, 20 hours
Re: Sending wedges in Micro Centrifuge tube [Re: tscott1632]
    #24909017 - 01/12/18 10:54 PM (9 months, 1 day ago)



--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineSolipsis
rooting for you
Male User Gallery


Registered: 12/28/09
Posts: 1,934
Loc: the Netherlands Flag
Last seen: 12 minutes, 51 seconds
Re: Sending wedges in Micro Centrifuge tube [Re: tiedma]
    #24910124 - 01/13/18 11:07 AM (9 months, 1 day ago)

These tubes tend to be PE or PP. I believe PP is a little better here...

Either way you'll wanna PC them by putting them in a filterlid jar or at least a jar with the lid sitting on loosely with aluminum foil over it, since you need to PC them open and protect against contam.

They should be able to just withstand this.

As for size: it can be a hassle to get multiple sticky wedges into a tube cleanly so don't get ones that are too tiny. On the other hand you want them to fit fine in a protected envelope without it getting too thick. Still, 2 ml is fine... if they really get too big they are probably not 'micro' centrifuge tubes anymore but rather like culture tubes of some sort which can still be designed for centrifugation.


--------------------
Trade List 


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinetscott1632
Shroomery Creeper
 User Gallery

Registered: 10/14/13
Posts: 243
Loc: Montana
Last seen: 7 days, 16 hours
Re: Sending wedges in Micro Centrifuge tube [Re: Solipsis]
    #24910604 - 01/13/18 01:25 PM (9 months, 22 hours ago)

Great, thanks for the advice. Now I just have to get some cultures to trade. LOL


--------------------
Trade List


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineMorePies
Stranger

Registered: 02/09/16
Posts: 775
Loc: TX Flag
Last seen: 34 minutes, 23 seconds
Re: Sending wedges in Micro Centrifuge tube [Re: tscott1632]
    #24912032 - 01/13/18 11:09 PM (9 months, 12 hours ago)

The ones in that link by tiedma are the ticket. Make sure anything you are going to autoclave is Polypropylene. Sometimes glass tubes won't have a PP cap and it's gross. Some other plastics can handle up to 12-14 psi at sea level, but there's so much polypropylene out there for our needs...just use that.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineSolipsis
rooting for you
Male User Gallery


Registered: 12/28/09
Posts: 1,934
Loc: the Netherlands Flag
Last seen: 12 minutes, 51 seconds
Re: Sending wedges in Micro Centrifuge tube [Re: MorePies]
    #24913134 - 01/14/18 12:22 PM (8 months, 30 days ago)

Definitely, I wouldn't gamble with PE (polyethylene)... it has a melting range (not point) of 115–135 °C (239–275 °F).

How hot your water can get without boiling and maxing out depends on the pressure your PC can manage. Some cooking PCs come with multiple vents like one for meat one for vegs that give you different pressures.

Buy PP to be safer since you stay under the melting range or otherwise if you are having problems cook without high pressure and just extend the cooking time to stay under the melting point.

Using jars also helps to be sure they stay away from the sides of the PC which can get a bit hotter. If there is any plastic at all in your PC use spacers or a jar etc to keep it away from the sides or it will easily melt.

Having culture tubes or something like that with poor thermoplasticity is such a bummer :frown: Especially if it is a nasty surprise


--------------------
Trade List 


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineQuadman
Challenged
Male User Gallery

Registered: 04/23/16
Posts: 1,900
Loc: IL Flag
Last seen: 1 hour, 41 minutes
Re: Sending wedges in Micro Centrifuge tube [Re: Solipsis]
    #24913560 - 01/14/18 03:32 PM (8 months, 30 days ago)

Not really a need to sterilize. If you're sending a culture out the other person gets the tube.:rolleyes: I can't think of a use otherwise for a 2 ml tube. I am assuming they come sterile.


--------------------
Rumors of my demise have been greatly exaggerated.
                                            Mark Twain
My glass is neither half full nor half empty, it is twice as big as it should be.


Edited by Quadman (01/14/18 03:33 PM)


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineHaywire
Wetspot Wizard
Male


Registered: 12/29/13
Posts: 866
Last seen: 5 days, 8 hours
Re: Sending wedges in Micro Centrifuge tube [Re: Quadman]
    #24914860 - 01/15/18 03:06 AM (8 months, 30 days ago)

Tubes are really great indeed, but I have been sending out wedges in ziplock bags.
Yes, sometimes they're a bit smushed when they arrive. 9/10 recover nicely though on agar.
However, centrifuge tubes are better for safe keeping. but just wanted to let you know they're alternatives.

Make sure upi check with the recipient if he's ok with this though.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineSolipsis
rooting for you
Male User Gallery


Registered: 12/28/09
Posts: 1,934
Loc: the Netherlands Flag
Last seen: 12 minutes, 51 seconds
Re: Sending wedges in Micro Centrifuge tube [Re: Haywire]
    #24914978 - 01/15/18 06:09 AM (8 months, 30 days ago)

If you buy cheap tubes on eBay like i do you can get them open, often.. and not really that well packaged necessarily. In any case I feel like I need to sterilize, however annoying it is since they were bought as 'sterile'.

How clean are ziploc bags?


--------------------
Trade List 


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinedeadmandave
Slime
Male User Gallery

Registered: 02/16/10
Posts: 1,500
Loc: NE CO Flag
Last seen: 29 days, 16 hours
Re: Sending wedges in Micro Centrifuge tube [Re: Solipsis]
    #24915828 - 01/15/18 03:48 PM (8 months, 29 days ago)

Ziplocs are very clean. I use them instead of Petri dishes for all my agar work. Probably been through 500 and I have contamination rate equal to my use of Petri dishes.



Post Extras: Print Post  Remind Me! Notify Moderator
Offlinetiedma
Fat Kid
Male

Registered: 08/16/17
Posts: 119
Last seen: 4 days, 20 hours
Re: Sending wedges in Micro Centrifuge tube [Re: deadmandave]
    #24915936 - 01/15/18 04:30 PM (8 months, 29 days ago)

:takingnotes:


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleAyePlus
Permycologist
I'm a teapot

Registered: 12/18/14
Posts: 702
Loc: PNW
Re: Sending wedges in Micro Centrifuge tube [Re: deadmandave]
    #24916045 - 01/15/18 05:15 PM (8 months, 29 days ago)

Quote:

deadmandave said:
Ziplocs are very clean. I use them instead of Petri dishes for all my agar work. Probably been through 500 and I have contamination rate equal to my use of Petri dishes.





:friendsshocked:

:freshwtf:

Kinda makes sense I guess If you rarely do agar and never do more than a few but damn is that awkward looking, I’ll stick with nice clean stackable breathable petris. And If I ever downscale dramatically I’ll use no pours with glad mini rounds or mini ziploc pp5s. Then If I feel the need I’ll put these in baggies.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineHaywire
Wetspot Wizard
Male


Registered: 12/29/13
Posts: 866
Last seen: 5 days, 8 hours
Re: Sending wedges in Micro Centrifuge tube [Re: deadmandave]
    #24917271 - 01/16/18 02:04 AM (8 months, 29 days ago)

Quote:

deadmandave said:
Ziplocs are very clean. I use them instead of Petri dishes for all my agar work. Probably been through 500 and I have contamination rate equal to my use of Petri dishes.






that's fucking ghetto mycology right there.
but I like it! a fine example of thinking outside of the box!


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineFunguy182
Stranger

Registered: 09/04/17
Posts: 405
Last seen: 18 days, 23 hours
Re: Sending wedges in Micro Centrifuge tube [Re: Haywire]
    #24917907 - 01/16/18 12:01 PM (8 months, 28 days ago)

LMAO. That's one way to do it. Before I had the money to keep up my Petri dish stock I used these little square Tupperware. You can find them at family Dollar in the kitchen utensils section eight come in a pack for a dollar I bought like ten packs of those and they worked awesome. Cheaper than ziplock bags and they look nice too. I drilled a tiny hole in the lid and covered the hole with micro pore tape. The cool thing about these little tupperware's is that they came in a variety of colors well the lids came in a variety of colors so you could color code all your species


Edited by Funguy182 (01/16/18 12:04 PM)


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineFunguy182
Stranger

Registered: 09/04/17
Posts: 405
Last seen: 18 days, 23 hours
Re: Sending wedges in Micro Centrifuge tube [Re: Funguy182]
    #24917919 - 01/16/18 12:07 PM (8 months, 28 days ago)

I do like the creativity with the bags but don't seem to be very cost efficient with agar. That's allot of agar I could get like six plates from one of those bags. Not knocking ya bro just saying. We all have our methods to our madness.


Edited by Funguy182 (01/16/18 12:08 PM)


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinedeadmandave
Slime
Male User Gallery

Registered: 02/16/10
Posts: 1,500
Loc: NE CO Flag
Last seen: 29 days, 16 hours
Re: Sending wedges in Micro Centrifuge tube [Re: Funguy182]
    #24918118 - 01/16/18 01:43 PM (8 months, 28 days ago)

$1.50 for 40 bags. Not many cheaper options and I like the convenience. I get about 25 plates per quart of agar and that's about the same as Petris if I remember right.

A little awkward yes, trying to navigate a hot scalpel in a plastic baggie is like playing operation. On the plus side you can smash a fully colonized baggie and then pour a bunch of little pieces into your jars almost like a g2g. And no parafilm :biggrin:

Sorry for the thread jack, my point is they're clean if you wanna use them for sending wedges.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleSpeeker

Registered: 02/11/04
Posts: 688
Re: Sending wedges in Micro Centrifuge tube [Re: deadmandave]
    #24918766 - 01/16/18 06:29 PM (8 months, 28 days ago)

For sending wedges ziplocs are fine. For agar work just use plates.
I'm all into new ideas, and but the plates are so much better.
Plain PS plates, not those you might want to re-use. When done just bin them.


--------------------
mushroom links, videos, etc.  blog


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Shop: MushCult - Live Mushroom Cultures Edible and Medicinal Mushroom Cultures on Agar   Amazon ½ Pint Jars, Agar, Autoclave, Parafilm, Petri Dish

Mushrooms, Mycology and Psychedelics >> Gourmet and Medicinal Mushrooms

Similar ThreadsPosterViewsRepliesLast post
* Lion's mane wedge charlieXxXxX 2,614 18 05/07/04 02:51 PM
by Speeker
* Cloning a wedge. What about your experience? FreeSporePrints 1,228 9 08/09/07 07:29 PM
by Jeremy_Davis
* Freesporeprints.org - 10 Pholiota nameko + 03 Lentinus edodes wedges FreeSporePrints 741 1 03/20/06 03:45 AM
by FreeSporePrints
* Advice Needed For Sending Cultures Jackal 1,646 14 03/26/03 08:10 PM
by FocusHawaii
* VVVVVV gets a free wedge thread!
( 1 2 3 4 ... 88 89 )
Raadt 259,122 1,776 07/27/18 12:44 AM
by Da Great MG
* Who wants some Pleurotus Pulmonarius wedges?
( 1 2 all )
puggymalone 3,901 21 02/23/04 09:28 AM
by ragadinks
* VVV Gets a free morel wedge or spore sample VVV YidakiMan 1,212 3 08/30/06 05:04 PM
by phalcon005
* Intro. & Need a Maitake wedge? booknik 987 4 01/25/05 10:03 PM
by jmeuser

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, cronicr, Pastywhyte
343 topic views. 1 members, 4 guests and 1 web crawlers are browsing this forum.
[ Print Topic ]
Search this thread:
Mushrooms.com
Please support our sponsors.

Copyright 1997-2018 Mind Media. Some rights reserved.

Generated in 0.047 seconds spending 0.008 seconds on 19 queries.