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blkbst
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Are all strains white? Is this contaminated? Help (pics)
#24904034 - 01/10/18 09:02 PM (6 years, 2 months ago) |
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First time cultivating, made agar with honey and potato flakes. Poured 3 Petri dishes (i'm using plastic ones). 2 dishes look somewhat similar, and one is a total mess, but looking at the photos here on the forums the mycelium doesn't look at all like that. At the same time this green thing is present on the 3 dishes, so i guess that's what i got from the syringe. Any ideas what to expect here? Is this green thing contaminated or is it what i got on the syringe i bought?
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Just_A_Noob
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Re: Are all strains white? Is this contaminated? Help (pics) [Re: blkbst]
#24904040 - 01/10/18 09:04 PM (6 years, 2 months ago) |
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Looks like there is some myc in the second one but yeah all those are contaminated. Did you use a SAB?
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mushpunx
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Re: Are all strains white? Is this contaminated? Help (pics) [Re: Just_A_Noob]
#24904092 - 01/10/18 09:19 PM (6 years, 2 months ago) |
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That is absolutely mold.
Yea, describe your process a bit for us please
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Tormato
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Registered: 07/01/17
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Re: Are all strains white? Is this contaminated? Help (pics) [Re: mushpunx]
#24904099 - 01/10/18 09:22 PM (6 years, 2 months ago) |
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UGH! Trich and Pin mold
-------------------- Helpful Threads The Shroomery Store Tormato's Q&A Thread Post Questions Here or PM me! "Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!
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Philosopherski
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Re: Are all strains white? Is this contaminated? Help (pics) [Re: Tormato]
#24904106 - 01/10/18 09:24 PM (6 years, 2 months ago) |
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Sorry mate
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Subnet Mask
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Re: Are all strains white? Is this contaminated? Help (pics) [Re: Philosopherski]
#24904108 - 01/10/18 09:26 PM (6 years, 2 months ago) |
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Impressive.
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blkbst
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Re: Are all strains white? Is this contaminated? Help (pics) [Re: mushpunx]
#24904129 - 01/10/18 09:34 PM (6 years, 2 months ago) |
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I PCed the agar, poured it under a SAB, let it cool, and gave a drop or two from a spore vial i got from a store I didn't expect all 3 dishes to fail so badly i'll be extra careful next try thanks for the replies
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Chief_Slapaho
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Re: Are all strains white? Is this contaminated? Help (pics) [Re: blkbst]
#24904261 - 01/10/18 10:20 PM (6 years, 2 months ago) |
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Honestly, it's probably the spore solution that carried the mold. Assuming your technique was good. You can pretty much always assume that spore prints and thus spore solutions have some contaminates.
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UnsungHero
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Re: Are all strains white? Is this contaminated? Help (pics) [Re: Chief_Slapaho]
#24904272 - 01/10/18 10:26 PM (6 years, 2 months ago) |
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Check your sterile technique, step up your SAB game and give it another go! Don't give up it's part of the process.
-------------------- "The quieter you become, the more you are able to hear."
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ShiVersblood
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Re: Are all strains white? Is this contaminated? Help (pics) [Re: blkbst]
#24904371 - 01/10/18 11:09 PM (6 years, 2 months ago) |
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It looks blue
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DavieDonna
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Re: Are all strains white? Is this contaminated? Help (pics) [Re: ShiVersblood] 1
#24904717 - 01/11/18 02:15 AM (6 years, 2 months ago) |
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That's some hardcore trich. Makes me angry just looking at it.
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LemurLemur
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Re: Are all strains white? Is this contaminated? Help (pics) [Re: blkbst]
#24904747 - 01/11/18 03:02 AM (6 years, 2 months ago) |
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In that first one is that were u put your spore solution?? If so its the syringe, try a print i find it much much much better than spore solution, i think syringes are for making cakes.
Infact try cakes or grains through SHIPS to get some mycilium then put a sample to agar and tranfer away.
Get a close up of that second 1 with the flash from underneath and on top, with so much tams u wanna be sure.
Spray the inside of your sab with soapy water with fine mist wait for it to settle and slowly move your hands u should move as fast as a chameleon, dont open your dishes more than u need n only open the dish sleeve in the sab, just speeze them in through a arm hole like cookie doe from 1 end. Look up Bod's n c10's agar guides.
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Boxer
Gasoline Fight Champion
Registered: 07/23/17
Posts: 133
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Re: Are all strains white? Is this contaminated? Help (pics) [Re: LemurLemur]
#24904777 - 01/11/18 04:29 AM (6 years, 2 months ago) |
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The first plate looks like penicillium, the 2nd has trich & pin mold and that's mycelium on the left. The third has trich and the white blotches are bacterial. Based on how wide spread the contamination is I would say it's you're syringe that's contaminated. You won't get anything clean from it more than likely. if getting another print/syringe isn't an option, I would transfer from the mycelium on the 2nd plate and see if you can clean it up via several transfers.
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blkbst
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Re: Are all strains white? Is this contaminated? Help (pics) [Re: Boxer]
#24905947 - 01/11/18 04:44 PM (6 years, 2 months ago) |
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I'm doing another pour right now, waiting for the agar to cool. I'll try getting a piece of the "mycelium" in the second one and put it in 2 new dishes, and try again with the syringe to see if it really is contaminated I'll update next week after this new pour grows
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Cakewonder
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Re: Are all strains white? Is this contaminated? Help (pics) [Re: blkbst]
#24906048 - 01/11/18 05:36 PM (6 years, 2 months ago) |
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Quote:
blkbst said: I PCed the agar, poured it under a SAB, let it cool, and gave a drop or two from a spore vial i got from a store I didn't expect all 3 dishes to fail so badly i'll be extra careful next try thanks for the replies
He used a SAB
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Edited by Cakewonder (01/11/18 10:47 PM)
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mushpunx
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Re: Are all strains white? Is this contaminated? Help (pics) [Re: Cakewonder]
#24906070 - 01/11/18 05:47 PM (6 years, 2 months ago) |
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Quote:
Cakewonder said:
Quote:
blkbst said: I PCed the agar, poured it under a SAB, let it cool, and gave a drop or two from a spore vial i got from a store I didn't expect all 3 dishes to fail so badly i'll be extra careful next try thanks for the replies
It is close to impossible to do pour in open air. Use the NOPOUR TEK.
? He said he poured it in a still air box, not open air. You can have the same success rate pouring in a SAB as in front of a flow hood. I learned in a SAB.
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streamer
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Re: Are all strains white? Is this contaminated? Help (pics) [Re: mushpunx]
#24906177 - 01/11/18 06:43 PM (6 years, 2 months ago) |
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I'd also pour an extra plate to see if the contams are coming from 'the pour' itself. A single drop of MS in the center is all that's needed. Your contams seem very widespread. Good luck!
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SimpleFarmer
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Re: Are all strains white? Is this contaminated? Help (pics) [Re: streamer]
#24906326 - 01/11/18 07:41 PM (6 years, 2 months ago) |
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How long are you pcing?
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Cakewonder
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Registered: 12/14/17
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Re: Are all strains white? Is this contaminated? Help (pics) [Re: SimpleFarmer]
#24906689 - 01/11/18 10:34 PM (6 years, 2 months ago) |
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I should sau sorry, I am no agar expert, there are other people who know this better and I should leave it to them to help him. I am sorry.
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Edited by Cakewonder (01/11/18 11:00 PM)
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blkbst
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Re: Are all strains white? Is this contaminated? Help (pics) [Re: SimpleFarmer]
#24907297 - 01/12/18 07:55 AM (6 years, 2 months ago) |
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Quote:
SimpleFarmer said: How long are you pcing?
45~60 mins. My PC doesn't make it to 15 psi, it only goes up to ~11 and I'm a bit reluctant to tape some coins on the valve trying to get higher pressure.
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