Home | Community | Message Board


Mycohaus
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Kratom Powder for Sale, Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Bridgetown Botanicals CBD Edibles   Original Sensible Seeds Bulk Cannabis Seeds, High THC Strains   Amazon Agar, Laminar Flow Hood, Petri Dish

Jump to first unread post. Pages: 1 | 2 | Next >  [ show all ]
Offlineblkbst
Stranger
Registered: 12/27/17
Posts: 7
Last seen: 3 years, 1 month
Are all strains white? Is this contaminated? Help (pics)
    #24904034 - 01/10/18 11:02 PM (3 years, 3 months ago)

First time cultivating, made agar with honey and potato flakes. Poured 3 Petri dishes (i'm using plastic ones). 2 dishes look somewhat similar, and one is a total mess, but looking at the photos here on the forums the mycelium doesn't look at all like that. At the same time this green thing is present on the 3 dishes, so i guess that's what i got from the syringe.
Any ideas what to expect here? Is this green thing contaminated or is it what i got on the syringe i bought?








Post Extras: Filter  Print Post  Remind Me! Notify Moderator
InvisibleJust_A_Noob
Breathing
Male User Gallery


Registered: 12/30/16
Posts: 6,783
Loc: PNW
Trusted Cultivator
Re: Are all strains white? Is this contaminated? Help (pics) [Re: blkbst]
    #24904040 - 01/10/18 11:04 PM (3 years, 3 months ago)

Looks like there is some myc in the second one but yeah all those are contaminated.  Did you use a SAB?


--------------------

Wearing a mask is bad for my physical, emotional, and spiritual health.
Complying = Consent
Wide Mouth 1/2 Pint No-Pour TEK
TC Teks & Links


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlinemushpunx
Fungus Punk
 User Gallery


Registered: 04/20/14
Posts: 13,322
Last seen: 12 hours, 2 minutes
Trusted Cultivator
Re: Are all strains white? Is this contaminated? Help (pics) [Re: Just_A_Noob]
    #24904092 - 01/10/18 11:19 PM (3 years, 3 months ago)

That is absolutely mold.

Yea, describe your process a bit for us please


--------------------

Amateur Mycologists United
AMU Q&A


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineTormato
It’s OK!! I’m a Limo Driver!! 😁
Male User Gallery


Folding@home Statistics
Registered: 07/01/17
Posts: 5,695
Last seen: 4 days, 23 hours
Trusted Cultivator
Re: Are all strains white? Is this contaminated? Help (pics) [Re: mushpunx]
    #24904099 - 01/10/18 11:22 PM (3 years, 3 months ago)

UGH! Trich and Pin mold :undecided:


--------------------
Helpful Threads

The Shroomery Store

Tormato's Q&A Thread Post Questions Here or PM me!

"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead

Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!

Tormato's LAGM 2020

Come chat with me and other Trusted Cultivators and Trusted Identifiers on Shroomery Discord


Some cool videos I like to watch under the influence.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflinePhilosopherski
Love is the meaning of life
 User Gallery


Registered: 11/17/17
Posts: 85
Loc: NYC Flag
Last seen: 1 year, 4 months
Re: Are all strains white? Is this contaminated? Help (pics) [Re: Tormato]
    #24904106 - 01/10/18 11:24 PM (3 years, 3 months ago)

Sorry mate :frown:


--------------------
Don't panic!
Always remember to bring a towel.

National Suicide Prevention Lifeline
Call 1-800-273-8255


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
InvisibleSubnet Mask
 User Gallery

Registered: 05/08/15
Posts: 902
Loc: Flag
Re: Are all strains white? Is this contaminated? Help (pics) [Re: Philosopherski]
    #24904108 - 01/10/18 11:26 PM (3 years, 3 months ago)

Impressive.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlineblkbst
Stranger
Registered: 12/27/17
Posts: 7
Last seen: 3 years, 1 month
Re: Are all strains white? Is this contaminated? Help (pics) [Re: mushpunx]
    #24904129 - 01/10/18 11:34 PM (3 years, 3 months ago)

I PCed the agar, poured it under a SAB, let it cool, and gave a drop or two from a spore vial i got from a store
I didn't expect all 3 dishes to fail so badly :frown: i'll be extra careful next try
thanks for the replies :smile:


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineChief_Slapaho
Outlaw

Registered: 12/07/17
Posts: 16
Last seen: 6 months, 19 days
Re: Are all strains white? Is this contaminated? Help (pics) [Re: blkbst]
    #24904261 - 01/11/18 12:20 AM (3 years, 3 months ago)

Honestly, it's probably the spore solution that carried the mold. Assuming your technique was good. You can pretty much always assume that spore prints and thus spore solutions have some contaminates.


--------------------
You think you're better than because you play by the rules? Who's rules? My life is my own. I'm an outlaw


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineUnsungHero
Endure & Persist


Registered: 11/21/17
Posts: 495
Loc: root@remotehost
Last seen: 1 year, 6 months
Re: Are all strains white? Is this contaminated? Help (pics) [Re: Chief_Slapaho]
    #24904272 - 01/11/18 12:26 AM (3 years, 3 months ago)

Check your sterile technique, step up your SAB game and give it another go! Don't give up it's part of the process. :cookiemonster:


--------------------
"The quieter you become, the more you are able to hear."


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineShiVersblood
VAmPiRES HELLA
I'm a teapot User Gallery


Registered: 08/19/07
Posts: 114,256
Loc: United States of America Flag
Last seen: 2 days, 16 hours
Re: Are all strains white? Is this contaminated? Help (pics) [Re: blkbst]
    #24904371 - 01/11/18 01:09 AM (3 years, 3 months ago)

It looks blue


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineDavieDonna
Stranger


Registered: 11/08/17
Posts: 18
Loc: Shattered Heavens
Last seen: 3 years, 3 months
Re: Are all strains white? Is this contaminated? Help (pics) [Re: ShiVersblood] * 1
    #24904717 - 01/11/18 04:15 AM (3 years, 3 months ago)

That's some hardcore trich.  Makes me angry just looking at it.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
InvisibleLemurLemur
Pray for Boog
 User Gallery


Registered: 01/30/17
Posts: 6,004
Loc: Drinking on the roof Flag
Re: Are all strains white? Is this contaminated? Help (pics) [Re: blkbst]
    #24904747 - 01/11/18 05:02 AM (3 years, 3 months ago)

In that first one is that were u put your spore solution?? If so its the syringe, try a print i find it much much much better than spore solution, i think syringes are for making cakes.

Infact try cakes or grains through SHIPS to get some mycilium then put a sample to agar and tranfer away.

Get a close up of that second 1 with the flash from underneath and on top, with so much tams u wanna be sure.

Spray the inside of your sab with soapy water with fine mist wait for it to settle and slowly move your hands u should move as fast as a chameleon, dont open your dishes more than u need n only open the dish sleeve in the sab, just speeze them in through a arm hole like cookie doe from 1 end.
Look up Bod's n c10's agar guides.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
InvisibleBoxer
Gasoline Fight Champion
 User Gallery

Registered: 07/23/17
Posts: 133
Re: Are all strains white? Is this contaminated? Help (pics) [Re: LemurLemur]
    #24904777 - 01/11/18 06:29 AM (3 years, 3 months ago)

The first plate looks like penicillium, the 2nd has trich & pin mold and that's mycelium on the left. The third has trich and the white blotches are bacterial.
Based on how wide spread the contamination is I would say it's you're syringe that's contaminated. You won't get anything clean from it more than likely.
if getting another print/syringe isn't an option, I would transfer from the mycelium on the 2nd plate and see if you can clean it up via several transfers.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlineblkbst
Stranger
Registered: 12/27/17
Posts: 7
Last seen: 3 years, 1 month
Re: Are all strains white? Is this contaminated? Help (pics) [Re: Boxer]
    #24905947 - 01/11/18 06:44 PM (3 years, 3 months ago)

I'm doing another pour right now, waiting for the agar to cool.
I'll try getting a piece of the "mycelium" in the second one and put it in 2 new dishes, and try again with the syringe to see if it really is contaminated
I'll update next week after this new pour grows :laugh:


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineCakewonder
Cake Mastah!
Male


Registered: 12/14/17
Posts: 487
Loc: North
Last seen: 9 months, 28 days
Re: Are all strains white? Is this contaminated? Help (pics) [Re: blkbst]
    #24906048 - 01/11/18 07:36 PM (3 years, 3 months ago)

Quote:

blkbst said:
I PCed the agar, poured it under a SAB, let it cool, and gave a drop or two from a spore vial i got from a store
I didn't expect all 3 dishes to fail so badly :frown: i'll be extra careful next try
thanks for the replies :smile:




He used a SAB


Edited by Cakewonder (01/12/18 12:47 AM)


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlinemushpunx
Fungus Punk
 User Gallery


Registered: 04/20/14
Posts: 13,322
Last seen: 12 hours, 2 minutes
Trusted Cultivator
Re: Are all strains white? Is this contaminated? Help (pics) [Re: Cakewonder]
    #24906070 - 01/11/18 07:47 PM (3 years, 3 months ago)

Quote:

Cakewonder said:
Quote:

blkbst said:
I PCed the agar, poured it under a SAB, let it cool, and gave a drop or two from a spore vial i got from a store
I didn't expect all 3 dishes to fail so badly :frown: i'll be extra careful next try
thanks for the replies :smile:




It is close to impossible to do pour in open air. Use the NOPOUR TEK.




? He said he poured it in a still air box, not open air. You can have the same success rate pouring in a SAB as in front of a flow hood. I learned in a SAB.


--------------------

Amateur Mycologists United
AMU Q&A


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlinestreamer

Registered: 09/06/14
Posts: 173
Last seen: 1 year, 10 months
Re: Are all strains white? Is this contaminated? Help (pics) [Re: mushpunx]
    #24906177 - 01/11/18 08:43 PM (3 years, 3 months ago)

I'd also pour an extra plate to see if the contams are coming from 'the pour' itself. A single drop of MS in the center is all that's needed. Your contams seem very widespread.  Good luck!


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineSimpleFarmer
Male User Gallery


Registered: 04/04/12
Posts: 3,973
Loc: gumby land
Last seen: 3 years, 2 months
Re: Are all strains white? Is this contaminated? Help (pics) [Re: streamer]
    #24906326 - 01/11/18 09:41 PM (3 years, 3 months ago)

How long are you pcing?


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineCakewonder
Cake Mastah!
Male


Registered: 12/14/17
Posts: 487
Loc: North
Last seen: 9 months, 28 days
Re: Are all strains white? Is this contaminated? Help (pics) [Re: SimpleFarmer]
    #24906689 - 01/12/18 12:34 AM (3 years, 3 months ago)

I should sau sorry, I am no agar expert, there are other people who know this better and I should leave it to them to help him. I am sorry.


Edited by Cakewonder (01/12/18 01:00 AM)


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlineblkbst
Stranger
Registered: 12/27/17
Posts: 7
Last seen: 3 years, 1 month
Re: Are all strains white? Is this contaminated? Help (pics) [Re: SimpleFarmer]
    #24907297 - 01/12/18 09:55 AM (3 years, 3 months ago)

Quote:

SimpleFarmer said:
How long are you pcing?



45~60 mins. My PC doesn't make it to 15 psi, it only goes up to ~11 and I'm a bit reluctant to tape some coins on the valve trying to get higher pressure.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1 | 2 | Next >  [ show all ]

Shop: PhytoExtractum Kratom Powder for Sale, Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Bridgetown Botanicals CBD Edibles   Original Sensible Seeds Bulk Cannabis Seeds, High THC Strains   Amazon Agar, Laminar Flow Hood, Petri Dish

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* possible contamination(pics) tayua 1,247 8 11/19/02 03:44 PM
by lvleph
* is my casing contaminated? pic inside.. theseeker 1,391 9 05/02/02 10:19 AM
by Roadkill
* Re: Do these look contaminated (Pic 2 - 6) MindQuester 1,141 3 12/29/99 01:15 PM
by Anonymous
* Some ??? Strain, etc... (pics) fidget 931 11 04/21/02 09:15 PM
by fidget
* Re: Do these look contaminated (Pic 1 of 6) Anonymous 1,160 2 12/28/99 11:45 PM
by Anonymous
* Re: help with contamination (pic) lostlogic 1,364 5 04/05/01 07:41 AM
by lostlogic
* Contamination???? *pic* aum 792 1 02/23/02 05:56 PM
by NugDumper
* What happens if I case 2 cakes of differ. strain? mrdasani 1,293 10 03/13/02 11:29 AM
by yogithehoneybear

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, FooMan, mushboy, fahtster, LogicaL Chaos, 13shrooms, stonesun, wildernessjunkie, cronicr, Pastywhyte, bodhisatta
716 topic views. 50 members, 329 guests and 109 web crawlers are browsing this forum.
[ Print Topic ]
Search this thread:
Cannabis Seeds - Original Sensible Seeds
Please support our sponsors.

Copyright 1997-2021 Mind Media. Some rights reserved.

Generated in 0.038 seconds spending 0.011 seconds on 18 queries.