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OfflineAtomHeartMother99
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Pastyplate possible contam question?
    #24903687 - 01/10/18 06:02 PM (7 years, 7 days ago)

I apologize for being to lazy to take pics at this exact moment, but I have some pastyplates that I knocked up on 1/1/18 and I am seeing an odd color. I used green food coloring and towards the edge of the plates it looks kind of orange underneath the mycelium. I can't tell if the discoloration is from the myc growing down into the agar, or perhaps its bacterial? I did 8 plates and maybe 3 or 4 seem to have this issue. The mycelium on top of the agar looks fine though.

This is my first run with agar/pastyplates, also I am using brown rice flour instead of tater flakes in the recipe FWIW.

Thanks in advance!

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OfflinehamloafM
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Re: Pastyplate possible contam question? [Re: AtomHeartMother99]
    #24903692 - 01/10/18 06:05 PM (7 years, 7 days ago)

Pics.


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How I Get Stuff done. - My Reference Guide. - My Grows.

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Offlinemushpunx
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Re: Pastyplate possible contam question? [Re: AtomHeartMother99]
    #24903697 - 01/10/18 06:07 PM (7 years, 7 days ago)

Can't tell without a pic, but it sounds like bacteria.
You want to make a transfer as early as you can after germination, before the myc has a chance to run into any mold colonies or bacteria.

Take a pic


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OfflineAtomHeartMother99
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Re: Pastyplate possible contam question? [Re: mushpunx]
    #24903772 - 01/10/18 06:56 PM (7 years, 7 days ago)

Much like me, my phones camera hates to focus. Hope this helps!




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Offlinemushpunx
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Re: Pastyplate possible contam question? [Re: AtomHeartMother99]
    #24904076 - 01/10/18 09:15 PM (7 years, 7 days ago)

It doesn't really help man I can't see the culture

I suggest you take a couple transfers from the cleanest looking spots to new plates and watch them carefully for bacteria or mold


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InvisibleBoxer
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Re: Pastyplate possible contam question? [Re: mushpunx]
    #24904787 - 01/11/18 04:45 AM (7 years, 6 days ago)

A shot of the mycelium itself would be helpful as well, you can tell a bit from how it looks. Unfortunately that's one of the downsides of colored agar,
and why I prefer mine clear. Hard to say whether it's an affect caused by the food coloring, but if you're seeing what you think you are, it's not a good sign.

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