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OfflineCakewonder
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Re: PF Tek Procedure Questions [Re: C12H16N20]
    #24903082 - 01/10/18 12:58 PM (7 years, 7 days ago)

Quote:

gillettij said:
Actually the vermiculite that you put on top acts as a filter between the substrate that you are going to inoculate and the aluminum lid that you made there for eliminating contamination




See my Sig for filter jars!


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OfflineLogicaL ChaosM
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Re: PF Tek Procedure Questions [Re: delirrr]
    #24903096 - 01/10/18 01:05 PM (7 years, 7 days ago)

delirrr asked:
I have some clarification questions for the first half of the PF Tek.

I will outline my understanding of the procedure, and note any questions I have.

(1) Mix Vermiculite with water, then add brown rice flour. Ratio is 2:1:1

Q: Does it matter if the BRF is organic, store bought, or grinder from brown rice?

- Doesnt matter really. I prefer storebought, standard BRF. Its smoother/denser.

Q: Does it matter if I use distilled or tap water?

- Nope, use whatever. Some people use pond water even. Tap is fine thou. I use bottled.


(2) Place BRF mixture into half-pint wide-mouthed jars. Add dry vermiculite layer.

Q: Is it correct that I have to just lightly place the mixture in jars. In other words, is it very important to leave the mixture with lots of space between particles?

Yeah, but I try to make it look like a cake, as in "one piece". Makes colonization easier that way.

(3) Place foil over lids, pressure cook for 90 minutes (ideally at 15 psi).

Q: Why is it so important to create a sterilized environment in the jars?

- TO sterilize the jar for proper mycelium growth.

Q: Won't water get into the jars even with the foil on?

No, not if its only steam.

(4) Once pressure cooking is completed, leave jars in the PC over night so that substrate cools off.

Q: Won't bacteria start to form, and creep into the jars?

- No, its sterile and some positive pressure too.

(5) Disinfect the SAB. Remove foil, and inoculate under SAB.

I have many questions about inoculation.

Q: Is this the correct procedure: Flame the needle until it is red. Let it cool for a second or two. Insert needle through lid hole at an angle, make sure needle is below the dry very layer and the tip is touching the inside glass. Squeeze 1/4 cc. Repeat for all holes. Wipe off with alcohol soaked paper towel. Re-sterilize needle by flaming it again. Repeat for other jars.

Most people flame their needles. I find it un-needed. Its up to you. Having a clean needle is important thou.

Q: What are we trying to achieve here? We are trying to get the spores to glob onto the substrate, correct? If I squeeze the solution towards the jar, won't some spores remain stuck to the sides of the glass?

Inject the spores into the jars. That simple. Try to get the solution on the cake insides near the glass. Thats it.

Q: how much of the needle needs to turn red? My last attempt I just flamed the tip.

- Thats fine, as long as its clean/sterile.

(6) Set the inoculated jars on a shelf and wait for like 2-3 weeks until they fully colonize.

I'm at this step with my second attempt at my first PF Tek. More questions to come if/when my latest jars show mycelium growth. Thanks.


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InvisibleZiran
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Re: PF Tek Procedure Questions [Re: LogicaL Chaos]
    #24903246 - 01/10/18 02:10 PM (7 years, 7 days ago)

It clearly says in my pftek guide to leave the foil off after inoculation.

Quote:

Ziran said:

We leave the foil off after inoculation for a few reasons.
  • The foil blocks Gas Exhange and can cause jars to stall
  • Condensation can form on the foil causing the dry verm layer to fail






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Offlinedelirrr
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Re: PF Tek Procedure Questions [Re: Ziran]
    #24903253 - 01/10/18 02:16 PM (7 years, 7 days ago)

Quote:

Ziran said:
It clearly says in my pftek guide to leave the foil off after inoculation.

Quote:

Ziran said:

We leave the foil off after inoculation for a few reasons.
  • The foil blocks Gas Exhange and can cause jars to stall
  • Condensation can form on the foil causing the dry verm layer to fail









Yes.

you also wrote:

4. After your pressure cooker cycle has finished turn off your burner and allow to cool overnight.

Where the confusion came in for me was when exactly do I take off the foil?

Boddhisatta recommended taking the foil off after PC is completed, and the jars have come to 0 psi. But he also said I would probably be okay to just leave it on until shortly before inoculation.

Either way, the proof is in the jars... which have been incubating for only 3-4 days now... in time I will know if I did it right, or if I should seek another syringe source.

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InvisibleZiran
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Re: PF Tek Procedure Questions [Re: delirrr]
    #24903274 - 01/10/18 02:31 PM (7 years, 7 days ago)

Thats fair. I personally dont like to remove the foil till inoc because if the dry verm layer happens to get disturbed after removing it, that is also a vector for contamns to sneak their way in


--------------------
Song Of Healing
:super: Updated Pf Tek Guide :super:
Ziran's Teks
AMU Q&A Thread
The Chinese word for nature is zìrán and it means that of which is of itself.


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OfflineChickenFarmer
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Re: PF Tek Procedure Questions [Re: Ziran]
    #24903312 - 01/10/18 02:50 PM (7 years, 7 days ago)

Quote:

bodhisatta said:

Quote:

ChickenFarmer said:
This is painful to read





So are pointless off topic opinions :cookiemonster:







You're right. Couple things I learned the hard way. After you put your WET Brf material in the jar, wipe the exposed part of the jar with an ISO wipe or something like that. Like the inside rim. The reason being, if you have any wet crumbles stuck to the jar up there they can act as a bridge for contaminates around your dry verm layer. Maybe this isn't a big deal, but it's definitely slowed the ridiculous cobweb contains I was having.

When I put my foil on I like to spin the jar a little bit when I'm smashing the foil down. The foil needs to be pretty tight or you'll get water on top of your jars, IME. This is just what I've noticed and may not be a big deal either.


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:stoner:

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OfflineC12H16N20
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Re: PF Tek Procedure Questions [Re: AyePlus]
    #24904019 - 01/10/18 08:57 PM (7 years, 7 days ago)

Wrong. As soon as the pressure drops to zero you can open the PC, but be careful because its still hot as fuck in there and the steam will burn you, remove the jars and place them somewhere closely together so they don’t cool off to fast and crack. Fill the PC again and get it running asap. No need to wait any longer.




Zero? Is that as in absolute zero? or zero in farenheit?

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OfflineChickenFarmer
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Re: PF Tek Procedure Questions [Re: C12H16N20]
    #24905405 - 01/11/18 12:28 PM (7 years, 6 days ago)

Neither. Atmospheric zero. Not relating to tempature, pressure rather.

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InvisiblebodhisattaMDiscordReddit
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Re: PF Tek Procedure Questions [Re: ChickenFarmer]
    #24905406 - 01/11/18 12:30 PM (7 years, 6 days ago)

:lmafo:

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OfflineChickenFarmer
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Re: PF Tek Procedure Questions [Re: bodhisatta]
    #24905407 - 01/11/18 12:31 PM (7 years, 6 days ago)

I get the feeling that I've missed something.....


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:stoner:

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InvisiblebodhisattaMDiscordReddit
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Re: PF Tek Procedure Questions [Re: ChickenFarmer]
    #24905413 - 01/11/18 12:33 PM (7 years, 6 days ago)

You replied to it. Absolute zero lol

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OfflineChickenFarmer
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Re: PF Tek Procedure Questions [Re: bodhisatta]
    #24905459 - 01/11/18 01:04 PM (7 years, 6 days ago)

I know, but it wasn't even temperature related. Bad try at humor, I appologize.


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:stoner:

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InvisiblebodhisattaMDiscordReddit
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Re: PF Tek Procedure Questions [Re: ChickenFarmer]
    #24905463 - 01/11/18 01:11 PM (7 years, 6 days ago)

i was laughing at c12 and enjoyed your reply...

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OfflineC12H16N20
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Re: PF Tek Procedure Questions [Re: bodhisatta]
    #24917571 - 01/16/18 06:15 AM (7 years, 1 day ago)

Bod, the thing is that most people do not have a pressure guage on their PC.
Every single tek always recommends to let your jars stay in all night unless you have more jars to prepare, and in that case, you wait until the pc cools off.

Chicken Farmer's ego said-wrong!

Your PC will not get to any zero... not centigrade, not farenheit, end definitely not absolute zero-nor will you be able to read what pressure you are at if you haven't a guage.

As for the earlier comment of mine about the foil......it was meant in the vain of using no lid, just foil. ( it did appear as I was saying three layers plus the lid).

My info comes from one of the largest sponsors of this forum, as they had produced a step by step video on how to PF Tek.

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InvisiblebodhisattaMDiscordReddit
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Re: PF Tek Procedure Questions [Re: C12H16N20]
    #24917813 - 01/16/18 09:16 AM (7 years, 1 day ago)

Every cooker still has a safety popup. That's how you know you got to zero. Otherwise no one would know when it was ok to open it lol.

My tek says take your jars out once it hits zero. I can do three runs a day easily

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OfflineRockyB
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Re: PF Tek Procedure Questions [Re: bodhisatta]
    #24918243 - 01/16/18 12:57 PM (7 years, 1 day ago)

I read it as: once the PC allows you to open it, you're ok to do so .. just don't burn yourself.

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OfflinehamloafM
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Re: PF Tek Procedure Questions [Re: RockyB]
    #24918332 - 01/16/18 01:40 PM (7 years, 1 day ago)

Once the PC returns to atmospheric pressure the option to open PC is available.  If the PC is opened before returning to atmospheric pressure the grains will puke out their moisture.


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