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Thedogtaylor
Amateur Mycologist

Registered: 05/14/17
Posts: 131
Last seen: 1 month, 29 days
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Have a few agar questions, all help is appreciated!
#24894200 - 01/06/18 01:37 PM (7 years, 11 days ago) |
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So i have been looking over pastys easy agar tek for a while now and im gonna give it a go. Would it be ok if i made the pda and then immediately poured the mixture into a glass bottle? Then maybe and hour or 2 later put the bottle along with my plastic petri dishes and alchohal in my sab and just pour the mixture? Never done agar before, it seems confusing for the first time. Thanks, also if you have a really easy way to make pda and pour that would be amazing!
-------------------- ZaNdY
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MrRabbit-HMR
Professional Duck Washer


Registered: 08/20/17
Posts: 262
Loc: UK
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Re: Have a few agar questions, all help is appreciated! [Re: Thedogtaylor]
#24894205 - 01/06/18 01:40 PM (7 years, 11 days ago) |
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Agar should be poured under as near sterile conditions as possible. Additional steps and prolonged exposure to air is likely going to result in nasties on your plates...
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Thedogtaylor
Amateur Mycologist

Registered: 05/14/17
Posts: 131
Last seen: 1 month, 29 days
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Re: Have a few agar questions, all help is appreciated! [Re: MrRabbit-HMR]
#24894223 - 01/06/18 01:46 PM (7 years, 11 days ago) |
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Thanks for your reply! So i couldn't do what i said even if i used a steralized bottle and a sab? And ofcourse poured in the SAB? what do you use?
-------------------- ZaNdY
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MrRabbit-HMR
Professional Duck Washer


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Re: Have a few agar questions, all help is appreciated! [Re: Thedogtaylor]
#24894231 - 01/06/18 01:50 PM (7 years, 11 days ago) |
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If you're pouring plates, you want to mix your agar in a bottle, pressure cook it, 15psi for 30-40 minutes, wait for it to cool down take out and pour in your SAB if that's all you have.
If you only have a SAB, I'd look into doing no-pour agar plates.
Edited by MrRabbit-HMR (01/07/18 04:21 AM)
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Thedogtaylor
Amateur Mycologist

Registered: 05/14/17
Posts: 131
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Re: Have a few agar questions, all help is appreciated! [Re: MrRabbit-HMR]
#24894244 - 01/06/18 01:56 PM (7 years, 11 days ago) |
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Ok awesome, would you recommend just following pastywhites tek or do you have one? I only have a SAB but i was reading a pressure cooker will melt plastic dishes. Should i just order some glass ones?
-------------------- ZaNdY
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Mitchell1992
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Registered: 10/07/17
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Re: Have a few agar questions, all help is appreciated! [Re: Thedogtaylor]
#24894447 - 01/06/18 03:34 PM (7 years, 11 days ago) |
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Na man use some little jars I chuck 10g light mault extract with 10g agar into cold water bring to a simmer as stirring make sure it’s all dissolved into the water then I pour about 30ml to each jar and pc for 20mins. I bang a hole in the lid and cover with tape to help with condensation and wrap tight with foil to stop water entering the jars
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Mitchell1992
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Re: Have a few agar questions, all help is appreciated! [Re: Mitchell1992]
#24894449 - 01/06/18 03:34 PM (7 years, 11 days ago) |
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Sorry 500ml of water make sure the jars are off the bottom of pc
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,943
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Re: Have a few agar questions, all help is appreciated! [Re: Mitchell1992] 2
#24894460 - 01/06/18 03:41 PM (7 years, 11 days ago) |
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1 I mix my agar.
2 Put in glass container and microwave, CLOSELY watching until it reaches a boil. This is to make sure it is dissolved and properly mixed, I use MEA.
3 I then leave lid loose and cover with foil.
4 PC at 15 PSI for 30 min, NOT 90 that will fuck the agar up. Agar does not take the same time to be sterilized as a solid mass like grains.
5 Agar need to cool but NOT solidify. I like to put my bottle in 140F water, with a thermometer. I watch it and start pouring at around 120-125f. You can handle the glass at this temp but it will start going solid quick. If pouring a lot of plates maybe start a little warmer. To warm will cause condensation.
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TheMadHatter420
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Re: Have a few agar questions, all help is appreciated! [Re: MrRabbit-HMR] 1
#24894464 - 01/06/18 03:42 PM (7 years, 11 days ago) |
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Quote:
MrRabbit-HMR said: If you're pouring plates, you want to mix your agar in a bottle, pressure cook it, 15psi for 90minutes, wait for it to cool down take out and pour in your SAB if that's all you have.
If you only have a SAB, I'd look into doing no-pour agar plates.
Your time is wrong and plenty of our members pour in a SAB.
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SimpleFarmer



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Re: Have a few agar questions, all help is appreciated! [Re: TheMadHatter420] 1
#24894514 - 01/06/18 04:17 PM (7 years, 11 days ago) |
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verum subsequentis
seeker of truth



Registered: 03/22/16
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Re: Have a few agar questions, all help is appreciated! [Re: TheMadHatter420]
#24894583 - 01/06/18 04:40 PM (7 years, 11 days ago) |
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Quote:
TheMadHatter420 said:

1 I mix my agar.
2 Put in glass container and microwave, CLOSELY watching until it reaches a boil. This is to make sure it is dissolved and properly mixed, I use MEA.
3 I then leave lid loose and cover with foil.
4 PC at 15 PSI for 30 min, NOT 90 that will fuck the agar up. Agar does not take the same time to be sterilized as a solid mass like grains.
5 Agar need to cool but NOT solidify. I like to put my bottle in 140F water, with a thermometer. I watch it and start pouring at around 120-125f. You can handle the glass at this temp but it will start going solid quick. If pouring a lot of plates maybe start a little warmer. To warm will cause condensation.
I pour up to 80 plates at a time and start when the temp is 145 or 150
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,943
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Re: Have a few agar questions, all help is appreciated! [Re: verum subsequentis] 1
#24894923 - 01/06/18 07:11 PM (7 years, 11 days ago) |
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I did 10, I can see how you would need to start higher to get through all of them. Maybe I should start a but higher next time as I got some air bubbles trapped in them.
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verum subsequentis
seeker of truth



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Re: Have a few agar questions, all help is appreciated! [Re: TheMadHatter420]
#24894941 - 01/06/18 07:18 PM (7 years, 11 days ago) |
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Yeah, i have to start a little hot to get it done before it starts to solidify. luckily, i invested in a huge ass flowhood so i can move pretty quickly and comfortably. I only notice condensation problems when i start above 150.
also, I have found that the cure to air bubble is to swirl the agar when i take it out of the pc and then let it settle until i pour it. I always get a few bubbles in my last few plates but they don't really bother me.
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,943
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They were not terrible, and I was pouring in front of the flow hood, so I figured I let it get to close to solidify temp, and it just trapped them in there as it probably solidified in a couple seconds with the hood blowing on them.
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MrRabbit-HMR
Professional Duck Washer


Registered: 08/20/17
Posts: 262
Loc: UK
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Re: Have a few agar questions, all help is appreciated! [Re: TheMadHatter420]
#24895680 - 01/07/18 04:20 AM (7 years, 10 days ago) |
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Quote:
TheMadHatter420 said:
Quote:
MrRabbit-HMR said: If you're pouring plates, you want to mix your agar in a bottle, pressure cook it, 15psi for 90minutes, wait for it to cool down take out and pour in your SAB if that's all you have.
If you only have a SAB, I'd look into doing no-pour agar plates.
Your time is wrong and plenty of our members pour in a SAB.
This is correct, sorry, I was thinking grains. I PC my agar for 30-40mins. Though, if you leave it for longer, I don't forsee there being any issues?
Will edit above post
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MrRabbit-HMR
Professional Duck Washer


Registered: 08/20/17
Posts: 262
Loc: UK
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Re: Have a few agar questions, all help is appreciated! [Re: TheMadHatter420]
#24895681 - 01/07/18 04:24 AM (7 years, 10 days ago) |
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Quote:
TheMadHatter420 said:

4 PC at 15 PSI for 30 min, NOT 90 that will fuck the agar up. Agar does not take the same time to be sterilized as a solid mass like grains.
What specifically does it do to the agar if you leave it in there for too long? Never tried.
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SimpleFarmer



Registered: 04/04/12
Posts: 3,973
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Re: Have a few agar questions, all help is appreciated! [Re: MrRabbit-HMR]
#24895684 - 01/07/18 04:31 AM (7 years, 10 days ago) |
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Why are you telling other people to do stuff you have no clue on? From what I hear is it will cook agar but then again I have never pced for 90mins bc 20-30 is standard unless you are using grain water then 45mins
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Shroomway
It's me!



Registered: 07/05/17
Posts: 438
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Re: Have a few agar questions, all help is appreciated! [Re: MrRabbit-HMR]
#24895709 - 01/07/18 05:24 AM (7 years, 10 days ago) |
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Quote:
MrRabbit-HMR said: What specifically does it do to the agar if you leave it in there for too long? Never tried.
The sugars caramelize. Apart from that (conected though) the agar solution gets unnecessary dark
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,943
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Re: Have a few agar questions, all help is appreciated! [Re: Shroomway]
#24895729 - 01/07/18 05:46 AM (7 years, 10 days ago) |
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Water can steam out causing the agar to be stiffer than you want as well. 90 min would cause a fair deal of moisture loss in agar compared to 30-40 min.
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mushpunx
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Re: Have a few agar questions, all help is appreciated! [Re: TheMadHatter420]
#24895765 - 01/07/18 06:19 AM (7 years, 10 days ago) |
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You can pour in a Still Air Box, taller SABs are better.
It sounds like you want to start with no pour plates. Pasty uses Glad Minirounds, they are PP5 plastic and can be sterilized in a PC without melting.
Pasty's is a good tek , I use them a lot when I need to save on plates.
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hamloaf
Q-dood ®©™√



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Re: Have a few agar questions, all help is appreciated! [Re: Thedogtaylor]
#24895811 - 01/07/18 07:14 AM (7 years, 10 days ago) |
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Quote:
Thedogtaylor said: So i have been looking over pastys easy agar tek for a while now and im gonna give it a go. Would it be ok if i made the pda and then immediately poured the mixture into a glass bottle? Then maybe and hour or 2 later put the bottle along with my plastic petri dishes and alchohal in my sab and just pour the mixture? Never done agar before, it seems confusing for the first time. Thanks, also if you have a really easy way to make pda and pour that would be amazing!
Don't worry about pouring plates for now. Pasty's Tek is a no-pour Tek. Simply boil water, add the ingredients, mix, distribute evenly into Glad brand "mini-rounds".
10 g Potato Flakes. 5 g Dextrose. 7.5 g Agar. 500ml Water.
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Thedogtaylor
Amateur Mycologist

Registered: 05/14/17
Posts: 131
Last seen: 1 month, 29 days
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Re: Have a few agar questions, all help is appreciated! [Re: hamloaf]
#24896701 - 01/07/18 03:20 PM (7 years, 10 days ago) |
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Awesome, thanks for the help guys! I think i might just use pasty,s tek for the first time. See alot of people saying good things. Ham are those measurements per container? So if i was making lets say 10 i would use 100 grams of potato flakes,50grams of dextrose and 70.5 grams of agar correct?
-------------------- ZaNdY
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verum subsequentis
seeker of truth



Registered: 03/22/16
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Re: Have a few agar questions, all help is appreciated! [Re: Thedogtaylor]
#24896748 - 01/07/18 03:46 PM (7 years, 10 days ago) |
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Not correct. he's giving you a recipe for 500 ml of agar. I think i used to do about 20 ml per glad mini round.
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hamloaf
Q-dood ®©™√



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Re: Have a few agar questions, all help is appreciated! [Re: Thedogtaylor]
#24896813 - 01/07/18 04:17 PM (7 years, 10 days ago) |
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simply cut the above recipe in half.
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Thedogtaylor
Amateur Mycologist

Registered: 05/14/17
Posts: 131
Last seen: 1 month, 29 days
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Re: Have a few agar questions, all help is appreciated! [Re: hamloaf]
#24897925 - 01/08/18 05:30 AM (7 years, 9 days ago) |
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Ok awesome even better! I love the shroomery , so many people offer hell P and guidance. We have the best community!
-------------------- ZaNdY
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