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Tormato  
Chairman of the Bored




Registered: 07/01/17
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Re: Knots or Trich? [Re: cronicr] 1
#24894032 - 01/06/18 12:02 PM (7 years, 11 days ago) |
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Quote:
cronicr said: If ya mean toss...yes. Cant remember if their spores are actuallysmaller then most filters?
2-3.5 microns according to the net...I’m going with yes they are smaller than most commonly used filters in this hobby
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cronicr



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Re: Knots or Trich? [Re: Tormato] 1
#24894045 - 01/06/18 12:08 PM (7 years, 11 days ago) |
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Thsnks
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Tormato  
Chairman of the Bored




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Re: Knots or Trich? [Re: cronicr] 1
#24894051 - 01/06/18 12:13 PM (7 years, 11 days ago) |
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You bet!
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Tormato's Q&A Thread Post Questions Here or PM me!
"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead
Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!
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Ziran
The Hero of Time




Registered: 02/03/16
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Loc: Temple of Time
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Re: Knots or Trich? [Re: Tormato]
#24894137 - 01/06/18 01:00 PM (7 years, 11 days ago) |
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shiiit. SFDs are only 3 microns.
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Tormato  
Chairman of the Bored




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Re: Knots or Trich? [Re: Ziran] 1
#24894142 - 01/06/18 01:02 PM (7 years, 11 days ago) |
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Quote:
Ziran said: shiiit. SFDs are only 3 microns.
Yerp...And I've read that Tyvex can vary between 2-15 microns
-------------------- Helpful Threads
The Shroomery Store
Tormato's Q&A Thread Post Questions Here or PM me!
"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead
Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!
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Ziran
The Hero of Time




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Posts: 6,034
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Re: Knots or Trich? [Re: Tormato] 1
#24894153 - 01/06/18 01:08 PM (7 years, 11 days ago) |
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Well. Im glad I use unmodded lids for the most part
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The Chinese word for nature is zìrán and it means that of which is of itself.

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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,915
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Re: Knots or Trich? [Re: Ziran]
#24894248 - 01/06/18 01:58 PM (7 years, 11 days ago) |
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Did you know in the hepa filters you use for flow hoods the average gap between fibers is way larger than 0.3 microns
Quote:
The air space between HEPA filter fibres is typically much greater than 0.3 μm. The common assumption that a HEPA filter acts like a sieve where particles smaller than the largest opening can pass through is incorrect and impractical
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Ziran
The Hero of Time




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Nope. Sure didnt. Ive also never used one XD
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The Chinese word for nature is zìrán and it means that of which is of itself.

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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: Knots or Trich? [Re: Ziran]
#24894283 - 01/06/18 02:14 PM (7 years, 11 days ago) |
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I remember reading that not to long ago, right now I have 6mm hole in the center of the lid very tightly stuffed with polyfil and 4 layers of micropore tape, 2 on each side of the lid. The tape is of course tightly taped on pressing down the poly even further. This seems much more safe than just polyfil. And much more practical especially when it comes to shaking jars. I know this is not ideal but it´s the best I have at the moment.
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Edited by Mateja (01/06/18 02:15 PM)
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Mateja


Registered: 07/14/16
Posts: 7,948
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Re: Knots or Trich? [Re: Mateja]
#24894293 - 01/06/18 02:17 PM (7 years, 11 days ago) |
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Also I will try unmodded lids next. Ziran, do you keep the lid on loose during colonization? I don´t men to jack the thread sry, you can shoot me a PM Ziran when you have the time.
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Edited by Mateja (01/06/18 02:18 PM)
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Ziran
The Hero of Time




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Re: Knots or Trich? [Re: Mateja]
#24894299 - 01/06/18 02:19 PM (7 years, 11 days ago) |
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Quote:
Mateah said: Also I will try unmodded lids next. Ziran, do you keep the lid on loose during colonization? I don´t men to jack the thread sry, you can shoot me a PM Ziran when you have the time.
Yes. also loosen & foil when pcing.
-------------------- Song Of Healing
Updated Pf Tek Guide
Ziran's Teks
AMU Q&A Thread
The Chinese word for nature is zìrán and it means that of which is of itself.

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ballsalsa
Universally Loathed and Reviled



Registered: 03/11/15
Posts: 26,329
Loc: Foreign Lands
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Quote:
bodhisatta said: Did you know in the hepa filters you use for flow hoods the average gap between fibers is way larger than 0.3 microns
Quote:
The air space between HEPA filter fibres is typically much greater than 0.3 μm. The common assumption that a HEPA filter acts like a sieve where particles smaller than the largest opening can pass through is incorrect and impractical
Do you know of anyone who has success with UV sterilizers? Seems like one could just run one all the time in one''s workspace to keep viable airborn contams to a minimum but I dunno, maybe spores are too resilient to be inactivated that way.
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Mateja


Registered: 07/14/16
Posts: 7,948
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Quote:
Mateah said: Micropore tape
Quote:
stonesun said: Well I didn't measure the the pore sizes, instead took a piece of tape and mounted it on a slide. Hydrated some P. cubensis spores and with an inoculation loop mounted them on the tape. The gaps are huge comparing to cubensis spores, which are ~12-17µm. I circled the fibers with individual spores sticked to them. Sorry for the kinda crappy images, didn't put a whole lotta effort in it...
100X

400X

400X
Quote:
RogerRabbit said:
Quote:
sandman420 said: This is from a faq about 3M Micropore tape, from 3M themselves. from 3m
Quote:
we don't claim that the tapes filter or block microorganisms.
Quote:
Question: Is Micropore tape sterile? Can it be sterilized? Answer: 3M Surgical tapes are sold clean, not sterile.
seems like micropore tape is a shitty choice for mushroom cultivation.
Remember, there's how a product performs, and there's what the manufacturers lawyers make them say about how it performs to prevent liability.
Micropore tape works pretty good to cover the holes on brf jars, but of course after sterilization, you couldn't take the tape off and use it again. That's why they say it can't be autoclaved. Also, if the pores are small enough to filter bacteria, then they're small enough for our use.RR
Quote:
lipa said: I use 2-3 layers of micropore tape on a 1/8-1/4 inch hole on my lids for grain spawn. I have no issues with contaminates unless I am careless. I go through thousands of jars a year and only buy a new box maybe twice a year. A total of 24 bucks for 24 rolls. I think it works pretty good.
Lipa
https://www.shroomery.org/forums/showflat.php/Number/13643478#13643478
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keeno
enthusiast



Registered: 06/01/11
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Quote:
bodhisatta said: Assuming it is actually aspergillus its still a toss situation. Aspergillus should theoretically be easier to salt tho. Say you put it somewhere outside to salvage rather than burying it.
yeah I salted it and tucked away somewhere outside, just to see how it develops. these blobs are fascinating.
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keeno
enthusiast



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Posts: 2,696
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Re: Knots or Trich? [Re: keeno]
#24905025 - 01/11/18 08:47 AM (7 years, 6 days ago) |
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just thought I'd update on this one...
it's fucking Blobtown Arizona!

I thought that the Aspergillus was spreading from under the salt, and whacked more on, but it looks liek it could be bruising from having the water sucked out?

either way it's a mess

and it's growing slowly as it's in a spot that is generally about 60 degrees f
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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Re: Knots or Trich? [Re: keeno] 1
#24905133 - 01/11/18 09:50 AM (7 years, 6 days ago) |
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Interesting to say the least.
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keeno
enthusiast



Registered: 06/01/11
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it's mutant city alright!
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RockyB
A little more than newb



Registered: 11/30/17
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Re: Knots or Trich? [Re: keeno] 1
#24905639 - 01/11/18 02:37 PM (7 years, 6 days ago) |
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It's pretty cool if nothing else. It's nice to know even with the contamination and low temps it's still producing.
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Ziran
The Hero of Time




Registered: 02/03/16
Posts: 6,034
Loc: Temple of Time
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Re: Knots or Trich? [Re: RockyB] 1
#24905785 - 01/11/18 03:57 PM (7 years, 6 days ago) |
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Mutants!
-------------------- Song Of Healing
Updated Pf Tek Guide
Ziran's Teks
AMU Q&A Thread
The Chinese word for nature is zìrán and it means that of which is of itself.

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keeno
enthusiast



Registered: 06/01/11
Posts: 2,696
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Re: Knots or Trich? [Re: RockyB]
#24906037 - 01/11/18 05:29 PM (7 years, 6 days ago) |
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Quote:
RockyB said: It's pretty cool if nothing else. It's nice to know even with the contamination and low temps it's still producing.
well kinda.... that fat one is about 5 days old and still just as big as the last knuckle on my thumb... I think the rest of the blobs will join up and murder me in my sleep before I have anythin I can harvest
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