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OfflineBizziness
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Why is it so hard for me to make a damn spore syringe ??
    #24892710 - 01/05/18 09:43 PM (3 years, 3 months ago)

This is my second time making a spore syringe. Working inside of a still air box I sanitize my glass cup with 70% isopropyl and I always seem to get paper towel fibers leftover inside the cup which is inevitable. I tried so hard to get these microfibers out and somehow they end up inside my Spore syringe. now since its sanitized will the fibers affect my spores?

I used boiling water to sterilize my syringe, plunged 4 times into another cup then drew up distilled water that I have in a jar with a ship that I have pressure. cooked for 60 mins at 15psi.

Also do I have to sterilize the foil  before laying down my caps to be printed ?

This whole process is critical to learn. I need my seeds from my crop to regrow !! Please help


--------------------
Shroomery show me the way!!!


Edited by Bizziness (01/05/18 09:46 PM)


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OfflineBird_Guts
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Re: Why is it so hard for me to make a damn spore syringe ?? [Re: Bizziness]
    #24892725 - 01/05/18 09:50 PM (3 years, 3 months ago)

Quote:

Bizziness said:
This is my second time making a spore syringe. Working inside of a still air box I sanitize my glass cup with 70% isopropyl and I always seem to get paper towel fibers leftover inside the cup which is inevitable. I tried so hard to get these microfibers out and somehow they end up inside my Spore syringe. now since its sanitized will the fibers affect my spores?

I used boiling water to sterilize my syringe, plunged 4 times into another cup then drew up distilled water that I have in a jar with a ship that I have pressure. cooked for 60 mins at 15psi.

Also do I have to sterilize the foil  before laying down my caps to be printed ?

This whole process is critical to learn. I need my seeds from my crop to regrow !! Please help




What I did was

PC some water in a jar with the SHIP

Also PC a shot glass or small jar, something small you can PC and will hold a syringe worth of water

In your SAB scrape spores from print with a sterile tool of some sort into shot glass

Get your sterile water into the syringe to be used

Squirt water from syringe into shot glass with spores, shoot it hard enuff to mix the spores and water up, but not hard enuough to make a mess

You have spore syringe about as clean as you gonna get

Good luck

--BG--

Edit: better yet, get the water into syringe before you scrape spore into glass. And wrap the shot glass or whatever in foil


Edited by Bird_Guts (01/05/18 09:52 PM)


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OfflineKKVguitarist
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Re: Why is it so hard for me to make a damn spore syringe ?? [Re: Bizziness]
    #24892729 - 01/05/18 09:53 PM (3 years, 3 months ago)

Look up pasty whites agar, learn to do agar, i wipe foil with iso before making prints, but then scrape to agar, get a clean specimen and either make lc from that or inject water into pasty plate, scrape with needle, suck up. Inject into lc. My biggest advice, look up smuzbox/bag people are going to bash me im sure but its the best thing ive invested in besides a pc. I had alot of problems with still airboxes.

The fibers shouldnt make a difference, all you can do is try!


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OfflineBizziness
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Registered: 12/26/17
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Re: Why is it so hard for me to make a damn spore syringe ?? [Re: Bird_Guts]
    #24892736 - 01/05/18 09:57 PM (3 years, 3 months ago)

Quote:

Bird_Guts said:
Quote:

Bizziness said:
This is my second time making a spore syringe. Working inside of a still air box I sanitize my glass cup with 70% isopropyl and I always seem to get paper towel fibers leftover inside the cup which is inevitable. I tried so hard to get these microfibers out and somehow they end up inside my Spore syringe. now since its sanitized will the fibers affect my spores?

I used boiling water to sterilize my syringe, plunged 4 times into another cup then drew up distilled water that I have in a jar with a ship that I have pressure. cooked for 60 mins at 15psi.

Also do I have to sterilize the foil  before laying down my caps to be printed ?

This whole process is critical to learn. I need my seeds from my crop to regrow !! Please help




What I did was

PC some water in a jar with the SHIP

Also PC a shot glass or small jar, something small you can PC and will hold a syringe worth of water

In your SAB scrape spores from print with a sterile tool of some sort into shot glass

Get your sterile water into the syringe to be used

Squirt water from syringe into shot glass with spores, shoot it hard enuff to mix the spores and water up, but not hard enuough to make a mess

You have spore syringe about as clean as you gonna get

Good luck

--BG--

Edit: better yet, get the water into syringe before you scrape spore into glass. And wrap the shot glass or whatever in foil




Ok that all make a perfect sense pc'ing the shot glass to eliminate the paper towel fibers. Now the only thing is that I'm actual reusing a syringe. So how do I go about all that with the distilled water in the ship jar? Like how would I sterilize the syringe before hand?


--------------------
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OfflinecronicrM
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Re: Why is it so hard for me to make a damn spore syringe ?? [Re: KKVguitarist]
    #24892739 - 01/05/18 09:58 PM (3 years, 3 months ago)

Foil is sterile off the roll...
No need for alcohol at all just wrap your shot glass in foil your syringe in foil and your blade/tool in foil and pc for 15 minutes along with a jar of water


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineBird_Guts
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Re: Why is it so hard for me to make a damn spore syringe ?? [Re: cronicr]
    #24892742 - 01/05/18 09:59 PM (3 years, 3 months ago)

:whathesaid:

--BG--


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InvisibleApples in Mono
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Registered: 09/21/17
Posts: 3,144
Re: Why is it so hard for me to make a damn spore syringe ?? [Re: Bizziness]
    #24892748 - 01/05/18 10:01 PM (3 years, 3 months ago)

Skip the syringe; put the spores to agar


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OfflineBizziness
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I'm a teapot


Registered: 12/26/17
Posts: 112
Loc: northern america
Last seen: 2 days, 2 hours
Re: Why is it so hard for me to make a damn spore syringe ?? [Re: KKVguitarist]
    #24892754 - 01/05/18 10:03 PM (3 years, 3 months ago)

Quote:

KKVguitarist said:
Look up pasty whites agar, learn to do agar, i wipe foil with iso before making prints, but then scrape to agar, get a clean specimen and either make lc from that or inject water into pasty plate, scrape with needle, suck up. Inject into lc. My biggest advice, look up smuzbox/bag people are going to bash me im sure but its the best thing ive invested in besides a pc. I had alot of problems with still airboxes.

The fibers shouldnt make a difference, all you can do is try!




Just looked on Google can't find this smuzbox you speak of!


--------------------
Shroomery show me the way!!!


Edited by Bizziness (01/05/18 10:04 PM)


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OfflineBird_Guts
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Re: Why is it so hard for me to make a damn spore syringe ?? [Re: Apples in Mono]
    #24892755 - 01/05/18 10:04 PM (3 years, 3 months ago)

Maybe hommie is making BRF cakes

--BG--


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OfflineBizziness
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I'm a teapot


Registered: 12/26/17
Posts: 112
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Last seen: 2 days, 2 hours
Re: Why is it so hard for me to make a damn spore syringe ?? [Re: Bird_Guts]
    #24892764 - 01/05/18 10:08 PM (3 years, 3 months ago)

Yes i am making brf cakes. Everyone says agar. How do I start with agar? Some one send me some info. I don't even know where to begin with agar and lc. I have and idea about lc. (Put light corn syrup in a jar with fae and ship and inoculate to get live culture?) I'm 1 month new at all of this but I think I'm catching on quick


--------------------
Shroomery show me the way!!!


Edited by Bizziness (01/05/18 10:08 PM)


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OfflineBird_Guts
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Re: Why is it so hard for me to make a damn spore syringe ?? [Re: KKVguitarist]
    #24892767 - 01/05/18 10:09 PM (3 years, 3 months ago)

Quote:

KKVguitarist said:
Look up pasty whites agar, learn to do agar, i wipe foil with iso before making prints, but then scrape to agar, get a clean specimen and either make lc from that or inject water into pasty plate, scrape with needle, suck up. Inject into lc. My biggest advice, look up smuzbox/bag people are going to bash me im sure but its the best thing ive invested in besides a pc. I had alot of problems with still airboxes.

The fibers shouldnt make a difference, all you can do is try!




Not trying to be a penis head but everything except for ready pastys tek was pretty bad advise

Spore> LC is a horrible idea

I'm willing to bet whipeing foil before taking a print would make it more dirty than foil fresh off the roll

IDK what a smuzvox is, but SAB is simple and quite effective, maybe tour setup and/or technique is off

--BG--


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OfflineBird_Guts
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Registered: 05/10/17
Posts: 1,014
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Re: Why is it so hard for me to make a damn spore syringe ?? [Re: Bizziness]
    #24892772 - 01/05/18 10:10 PM (3 years, 3 months ago)

Quote:

Bizziness said:
Yes i am making brf cakes. Everyone says agar. How do I start with agar? Some one send me some info. I don't even know where to begin with agar and lc. I have and idea about lc. (Put light corn syrup in a jar with fae and ship and inoculate to get live culture?) I'm 1 month new at all of this but I think I'm catching on quick




Search engine at the top right

Don't be reading 12 year old teks, plenty of good up to date stuff in the last 2 years

--BG--


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InvisibleApples in Mono
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Registered: 09/21/17
Posts: 3,144
Re: Why is it so hard for me to make a damn spore syringe ?? [Re: Bird_Guts]
    #24892780 - 01/05/18 10:14 PM (3 years, 3 months ago)

If homie has prints, then i think he should do agar


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InvisibleApples in Mono
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Re: Why is it so hard for me to make a damn spore syringe ?? [Re: Bizziness]
    #24892785 - 01/05/18 10:16 PM (3 years, 3 months ago)

Follow pasty's easy agar tek and you'll have spores on agar tomorrow. You don't even need a pc. Although it is certainly recommended if possible


Edited by Apples in Mono (01/05/18 10:18 PM)


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OfflineBird_Guts
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Re: Why is it so hard for me to make a damn spore syringe ?? [Re: Apples in Mono]
    #24892796 - 01/05/18 10:19 PM (3 years, 3 months ago)

He's not doing grains> bulk, he's doing BRF cakes lol

--BG--


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InvisibleApples in Mono
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Re: Why is it so hard for me to make a damn spore syringe ?? [Re: Bird_Guts]
    #24892797 - 01/05/18 10:21 PM (3 years, 3 months ago)

Still best to do agar first. Plus he'll probably switch to grain by the time his cultures are ready


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OfflineBird_Guts
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Re: Why is it so hard for me to make a damn spore syringe ?? [Re: Apples in Mono]
    #24892801 - 01/05/18 10:24 PM (3 years, 3 months ago)

Quote:

Apples in Mono said:
Still best to do agar first. Plus he'll probably switch to grain by the time his cultures are ready




Well I personally agree, but for some people the PF tek is all they need or want.

I mean I never made a cake in my life but trusted sources say PF tek is good for noobs, so I figure just answer OPs question


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InvisibleApples in Mono
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Registered: 09/21/17
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Re: Why is it so hard for me to make a damn spore syringe ?? [Re: Bird_Guts]
    #24892808 - 01/05/18 10:28 PM (3 years, 3 months ago)

I hear ya. But if he's going through the trouble of making his own syringes then i bet we can talk him into pasty plates:smirk:


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OfflineBizziness
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Re: Why is it so hard for me to make a damn spore syringe ?? [Re: Apples in Mono]
    #24892817 - 01/05/18 10:32 PM (3 years, 3 months ago)

Yeah i just bought coco coir and thinking about doing small trays and putting them inside my sgfc to fruit. I understand you can't use agar with brf cakes because you dump the colonized agar into the grains jar and inoculate the brf jars with spore syringe and live culture . Correct?

What are some good grains to start off with for noobs?


--------------------
Shroomery show me the way!!!


Edited by Bizziness (01/05/18 10:33 PM)


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OfflinecronicrM
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Re: Why is it so hard for me to make a damn spore syringe ?? [Re: Bizziness]
    #24892819 - 01/05/18 10:34 PM (3 years, 3 months ago)

I still love cakes lol


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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