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keeno
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Knots or Trich?
#24891488 - 01/05/18 09:29 AM (7 years, 12 days ago) |
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howdy folks, happy Friday to you 
I've gotten the fear. This looks like trouble....

what do you think?
should I launch into orbit and nuke this one from space?
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Skizor1337
Trip King



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Re: Knots or Trich? [Re: keeno] 1
#24891500 - 01/05/18 09:36 AM (7 years, 12 days ago) |
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Quote:
keeno said: howdy folks, happy Friday to you 
I've gotten the fear. This looks like trouble....

what do you think?
should I launch into orbit and nuke this one from space? 
I am not a contam expert by any means but this could be contam
Here's my recent thread with very similar results. I had trouble taking pics but maybe you can reference it to see if it matches or not
https://www.shroomery.org/forums/showflat.php/Number/24881915
-------------------- 100% Professional Psychonaut
Trippin' Aint Easy
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An_Observer
Stranger


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Looks like trich in it's early stages. I'd nuke it from orbit. Wait for a TC to confirm though.
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keeno
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looks pretty close... did you see it turn green before you tossed it?
I've had a few folks suggesting that it's forming blobs and not trich... I'll report back with my findings
cheers Skizor
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bodhisatta 
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Re: Knots or Trich? [Re: keeno] 1
#24891572 - 01/05/18 10:27 AM (7 years, 12 days ago) |
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Looks like some ugly blob action right now. sometimes you'll get that from over misting and wet substrate
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keeno
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ah that's interesting, cheers Bod It's my 2nd mono and I've been trying to recreate comebackkids surface conditions, but I may well have over misted. cheers for your thoughts!
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Fascistbullyboy
Trich master



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Re: Knots or Trich? [Re: keeno] 1
#24891631 - 01/05/18 11:01 AM (7 years, 12 days ago) |
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It ain't trich that's for sure.
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keeno
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good news, cheers boss
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Skizor1337
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Re: Knots or Trich? [Re: keeno] 1
#24891997 - 01/05/18 02:32 PM (7 years, 12 days ago) |
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Quote:
keeno said: good news, cheers boss 
Yea dude I tossed it.
I would also toss yours. The one guy said that letting mold in your space will contaminate future projects.
I can't take that chance.
-------------------- 100% Professional Psychonaut
Trippin' Aint Easy
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bodhisatta 
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If trich substrates fucked a room me and a lot of others would be having a bunch of issues. Its poor form to keep moldy substrate around but its not like it fucks you
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Skizor1337
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Quote:
bodhisatta said: If trich substrates fucked a room me and a lot of others would be having a bunch of issues. Its poor form to keep moldy substrate around but its not like it fucks you
I used bleach to clean ceiling to floor haha. I may have went overkill then.
It's all good
-------------------- 100% Professional Psychonaut
Trippin' Aint Easy
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Mitchell1992
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How’s it looking now mate intrested to see how it gets on! Hope it’s all good
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Fascistbullyboy
Trich master



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This trich before sporulating
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bodhisatta 
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 About to go green
 Not trich
 Also not trich
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Fascistbullyboy
Trich master



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Keeno didn't you say in another post the other day that you was trying that moisture Tek(can't remember who you said posted it but he got that kid with chocolate on his face for an avatar)?
Then quoting bod in another post they could be blobs due to being too wet. And I'm sure bod will agree.
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Ziran
The Hero of Time




Registered: 02/03/16
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That is knot trich.
-------------------- Song Of Healing
Updated Pf Tek Guide
Ziran's Teks
AMU Q&A Thread
The Chinese word for nature is zìrán and it means that of which is of itself.

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Tormato  
Chairman of the Bored




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Re: Knots or Trich? [Re: Ziran] 2
#24892187 - 01/05/18 04:02 PM (7 years, 12 days ago) |
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I see what you did there 
Quote:
Ziran said: That is knot trich.
-------------------- Helpful Threads
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Tormato's Q&A Thread Post Questions Here or PM me!
"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead
Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!
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cronicr



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Re: Knots or Trich? [Re: Tormato] 1
#24892323 - 01/05/18 04:52 PM (7 years, 12 days ago) |
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Keeping mold around is not a great thing at all...no ot does not fuck you but having a clean area can be the difference between making past your first couple flushes. Substates get weak after fruiting so a high sporeload with a weak sub will often mean contams will win...vacuum your floors once in awhile
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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The Influence
Free Sheeks



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Quote:
bodhisatta said: If trich substrates fucked a room me and a lot of others would be having a bunch of issues. Its poor form to keep moldy substrate around but its not like it fucks you
I second this
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Tormato  
Chairman of the Bored




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Re: Knots or Trich? [Re: cronicr] 1
#24892386 - 01/05/18 05:14 PM (7 years, 12 days ago) |
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Quote:
cronicr said: Keeping mold around is not a great thing at all...no ot does not fuck you but having a clean area can be the difference between making past your first couple flushes. Substates get weak after fruiting so a high sporeload with a weak sub will often mean contams will win...vacuum your floors once in awhile

Pretty fair to say any precautions taken will increase your chances of future success
-------------------- Helpful Threads
The Shroomery Store
Tormato's Q&A Thread Post Questions Here or PM me!
"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead
Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!
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keeno
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Re: Knots or Trich? [Re: Tormato]
#24893584 - 01/06/18 07:26 AM (7 years, 11 days ago) |
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cheers for the input everyone!
any whilst the blobs haven't gone green, this has...

so it's gonna have to go out. shit shame it too cold for it to do its thing out there
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Fascistbullyboy
Trich master



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Re: Knots or Trich? [Re: keeno] 1
#24893593 - 01/06/18 07:31 AM (7 years, 11 days ago) |
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Shame. I bet I've got green somewhere it doesn't feel right if I ain't lol.
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Mitchell1992
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Ah man what a bummer just wasn’t meant to be! Better luck next time
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Tormato  
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Dammit...
-------------------- Helpful Threads
The Shroomery Store
Tormato's Q&A Thread Post Questions Here or PM me!
"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead
Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!
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keeno
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Re: Knots or Trich? [Re: Tormato]
#24893698 - 01/06/18 08:52 AM (7 years, 11 days ago) |
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cheers folks! I'm gonna move it out of my grow area and stick a bit of salt paste over the trich, see if I can't coax a flush...
but them's the breaks eh?
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RockyB
A little more than newb



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Re: Knots or Trich? [Re: keeno]
#24893708 - 01/06/18 08:58 AM (7 years, 11 days ago) |
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You sure that's not bruising? Or better, why are you sure it's not bruising?
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keeno
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Re: Knots or Trich? [Re: RockyB]
#24893715 - 01/06/18 09:05 AM (7 years, 11 days ago) |
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the colour's wrong IMO too green, not blue enough
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RockyB
A little more than newb



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Re: Knots or Trich? [Re: keeno]
#24893720 - 01/06/18 09:08 AM (7 years, 11 days ago) |
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Huh, it looks like early bruising to me. Before it goes dark enough to look blue. I had something similar and I freaked out, and someone said before you do anything take a cue tip and swipe it. If you have green residue it's bad, if not it's most likely bruising.
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bodhisatta 
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Re: Knots or Trich? [Re: RockyB] 1
#24893727 - 01/06/18 09:13 AM (7 years, 11 days ago) |
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Thats clearly not bruising lol.
Its probably aspergillus and not trich. A microscope would confirm. Trich usually has telltale white trich mycelium before the green shows. Aspergillus usually shows up like that from my experience
The qtip test is ridiculous. Mold grows on things. Bruising is a color change of mycelium. You can clearly tell its mold growing and not just a color change. Besides sometimes blue bruised mycelium makes the qtip blue and noobs get all freaked.
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RockyB
A little more than newb



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Quote:
bodhisatta said: Thats clearly not bruising lol.
Its probably aspergillus and not trich. A microscope would confirm. Trich usually has telltale white trich mycelium before the green shows. Aspergillus usually shows up like that from my experience
The qtip test is ridiculous. Mold grows on things. Bruising is a color change of mycelium. You can clearly tell its mold growing and not just a color change. Besides sometimes blue bruised mycelium makes the qtip blue and noobs get all freaked.
Dang, I'm just trying to understand all this, so I can understand some false positives, but do you ever have a false negative? Maybe I'm just dumb, but I can't seem to see the difference in characteristics.
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mushboy
modboy



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Re: Knots or Trich? [Re: RockyB] 1
#24893799 - 01/06/18 09:54 AM (7 years, 11 days ago) |
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trich
 notice the bruising around the trich. zoom in and inspect the grainy texture
asparagus
 nothing really grainy just green and gross looking. like the picture posted a few jawns up.
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bodhisatta 
Smurf real estate agent


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Re: Knots or Trich? [Re: mushboy] 1
#24893804 - 01/06/18 09:56 AM (7 years, 11 days ago) |
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I think your asparagus is bad with aspergillus
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mushboy
modboy



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maybe..
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Mateja


Registered: 07/14/16
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That jade/emerald green that aspergillus and trich produce is pretty hard to confuse with bruising IMO. When I harvest using scissors or knife the stumps usually turn dark green, but even then it´s pretty distinct from the aspergillus green.
-------------------- Cakes inside Water Tub
Edited by Mateja (01/06/18 10:08 AM)
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mushboy
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Re: Knots or Trich? [Re: Mateja]
#24893824 - 01/06/18 10:11 AM (7 years, 11 days ago) |
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ive noticed almost every lgt grow ive done bruises greenish blue.

bruising is a reaction. molds are just fucking molds.
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Tormato  
Chairman of the Bored




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Oopsy! It happens 
Quote:
bodhisatta said: I think your asparagus is bad with aspergillus
-------------------- Helpful Threads
The Shroomery Store
Tormato's Q&A Thread Post Questions Here or PM me!
"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead
Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!
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keeno
enthusiast



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Re: Knots or Trich? [Re: Tormato]
#24893993 - 01/06/18 11:44 AM (7 years, 11 days ago) |
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and is aspergillus to be treated the same as trich?
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mushboy
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Re: Knots or Trich? [Re: keeno] 1
#24894001 - 01/06/18 11:47 AM (7 years, 11 days ago) |
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i toss when i see it. my house is a loaded with aspergillus. it grows on juice cups left out, agar plates if im not careful. bread molds with it like right away if left out.
them bitches run deep.
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cronicr



Registered: 08/07/11
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Re: Knots or Trich? [Re: keeno] 1
#24894003 - 01/06/18 11:48 AM (7 years, 11 days ago) |
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If ya mean toss...yes. Cant remember if their spores are actuallysmaller then most filters?
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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bodhisatta 
Smurf real estate agent


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Re: Knots or Trich? [Re: keeno] 1
#24894004 - 01/06/18 11:48 AM (7 years, 11 days ago) |
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Assuming it is actually aspergillus its still a toss situation. Aspergillus should theoretically be easier to salt tho. Say you put it somewhere outside to salvage rather than burying it.
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Tormato  
Chairman of the Bored




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Re: Knots or Trich? [Re: cronicr] 1
#24894032 - 01/06/18 12:02 PM (7 years, 11 days ago) |
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Quote:
cronicr said: If ya mean toss...yes. Cant remember if their spores are actuallysmaller then most filters?
2-3.5 microns according to the net...I’m going with yes they are smaller than most commonly used filters in this hobby
-------------------- Helpful Threads
The Shroomery Store
Tormato's Q&A Thread Post Questions Here or PM me!
"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead
Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!
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cronicr



Registered: 08/07/11
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Re: Knots or Trich? [Re: Tormato] 1
#24894045 - 01/06/18 12:08 PM (7 years, 11 days ago) |
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Thsnks
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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Tormato  
Chairman of the Bored




Registered: 07/01/17
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Re: Knots or Trich? [Re: cronicr] 1
#24894051 - 01/06/18 12:13 PM (7 years, 11 days ago) |
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You bet!
-------------------- Helpful Threads
The Shroomery Store
Tormato's Q&A Thread Post Questions Here or PM me!
"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead
Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!
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Ziran
The Hero of Time




Registered: 02/03/16
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Loc: Temple of Time
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Re: Knots or Trich? [Re: Tormato]
#24894137 - 01/06/18 01:00 PM (7 years, 11 days ago) |
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shiiit. SFDs are only 3 microns.
-------------------- Song Of Healing
Updated Pf Tek Guide
Ziran's Teks
AMU Q&A Thread
The Chinese word for nature is zìrán and it means that of which is of itself.

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Tormato  
Chairman of the Bored




Registered: 07/01/17
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Re: Knots or Trich? [Re: Ziran] 1
#24894142 - 01/06/18 01:02 PM (7 years, 11 days ago) |
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Quote:
Ziran said: shiiit. SFDs are only 3 microns.
Yerp...And I've read that Tyvex can vary between 2-15 microns
-------------------- Helpful Threads
The Shroomery Store
Tormato's Q&A Thread Post Questions Here or PM me!
"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead
Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!
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Ziran
The Hero of Time




Registered: 02/03/16
Posts: 6,034
Loc: Temple of Time
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Re: Knots or Trich? [Re: Tormato] 1
#24894153 - 01/06/18 01:08 PM (7 years, 11 days ago) |
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Well. Im glad I use unmodded lids for the most part
-------------------- Song Of Healing
Updated Pf Tek Guide
Ziran's Teks
AMU Q&A Thread
The Chinese word for nature is zìrán and it means that of which is of itself.

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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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Re: Knots or Trich? [Re: Ziran]
#24894248 - 01/06/18 01:58 PM (7 years, 11 days ago) |
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Did you know in the hepa filters you use for flow hoods the average gap between fibers is way larger than 0.3 microns
Quote:
The air space between HEPA filter fibres is typically much greater than 0.3 μm. The common assumption that a HEPA filter acts like a sieve where particles smaller than the largest opening can pass through is incorrect and impractical
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Ziran
The Hero of Time




Registered: 02/03/16
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Nope. Sure didnt. Ive also never used one XD
-------------------- Song Of Healing
Updated Pf Tek Guide
Ziran's Teks
AMU Q&A Thread
The Chinese word for nature is zìrán and it means that of which is of itself.

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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: Knots or Trich? [Re: Ziran]
#24894283 - 01/06/18 02:14 PM (7 years, 11 days ago) |
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I remember reading that not to long ago, right now I have 6mm hole in the center of the lid very tightly stuffed with polyfil and 4 layers of micropore tape, 2 on each side of the lid. The tape is of course tightly taped on pressing down the poly even further. This seems much more safe than just polyfil. And much more practical especially when it comes to shaking jars. I know this is not ideal but it´s the best I have at the moment.
-------------------- Cakes inside Water Tub
Edited by Mateja (01/06/18 02:15 PM)
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Mateja


Registered: 07/14/16
Posts: 7,948
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Re: Knots or Trich? [Re: Mateja]
#24894293 - 01/06/18 02:17 PM (7 years, 11 days ago) |
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Also I will try unmodded lids next. Ziran, do you keep the lid on loose during colonization? I don´t men to jack the thread sry, you can shoot me a PM Ziran when you have the time.
-------------------- Cakes inside Water Tub
Edited by Mateja (01/06/18 02:18 PM)
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Ziran
The Hero of Time




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Re: Knots or Trich? [Re: Mateja]
#24894299 - 01/06/18 02:19 PM (7 years, 11 days ago) |
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Quote:
Mateah said: Also I will try unmodded lids next. Ziran, do you keep the lid on loose during colonization? I don´t men to jack the thread sry, you can shoot me a PM Ziran when you have the time.
Yes. also loosen & foil when pcing.
-------------------- Song Of Healing
Updated Pf Tek Guide
Ziran's Teks
AMU Q&A Thread
The Chinese word for nature is zìrán and it means that of which is of itself.

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ballsalsa
Universally Loathed and Reviled



Registered: 03/11/15
Posts: 26,329
Loc: Foreign Lands
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Quote:
bodhisatta said: Did you know in the hepa filters you use for flow hoods the average gap between fibers is way larger than 0.3 microns
Quote:
The air space between HEPA filter fibres is typically much greater than 0.3 μm. The common assumption that a HEPA filter acts like a sieve where particles smaller than the largest opening can pass through is incorrect and impractical
Do you know of anyone who has success with UV sterilizers? Seems like one could just run one all the time in one''s workspace to keep viable airborn contams to a minimum but I dunno, maybe spores are too resilient to be inactivated that way.
--------------------
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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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Quote:
Mateah said: Micropore tape
Quote:
stonesun said: Well I didn't measure the the pore sizes, instead took a piece of tape and mounted it on a slide. Hydrated some P. cubensis spores and with an inoculation loop mounted them on the tape. The gaps are huge comparing to cubensis spores, which are ~12-17µm. I circled the fibers with individual spores sticked to them. Sorry for the kinda crappy images, didn't put a whole lotta effort in it...
100X

400X

400X
Quote:
RogerRabbit said:
Quote:
sandman420 said: This is from a faq about 3M Micropore tape, from 3M themselves. from 3m
Quote:
we don't claim that the tapes filter or block microorganisms.
Quote:
Question: Is Micropore tape sterile? Can it be sterilized? Answer: 3M Surgical tapes are sold clean, not sterile.
seems like micropore tape is a shitty choice for mushroom cultivation.
Remember, there's how a product performs, and there's what the manufacturers lawyers make them say about how it performs to prevent liability.
Micropore tape works pretty good to cover the holes on brf jars, but of course after sterilization, you couldn't take the tape off and use it again. That's why they say it can't be autoclaved. Also, if the pores are small enough to filter bacteria, then they're small enough for our use.RR
Quote:
lipa said: I use 2-3 layers of micropore tape on a 1/8-1/4 inch hole on my lids for grain spawn. I have no issues with contaminates unless I am careless. I go through thousands of jars a year and only buy a new box maybe twice a year. A total of 24 bucks for 24 rolls. I think it works pretty good.
Lipa
https://www.shroomery.org/forums/showflat.php/Number/13643478#13643478
-------------------- Cakes inside Water Tub
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keeno
enthusiast



Registered: 06/01/11
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Quote:
bodhisatta said: Assuming it is actually aspergillus its still a toss situation. Aspergillus should theoretically be easier to salt tho. Say you put it somewhere outside to salvage rather than burying it.
yeah I salted it and tucked away somewhere outside, just to see how it develops. these blobs are fascinating.
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keeno
enthusiast



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Re: Knots or Trich? [Re: keeno]
#24905025 - 01/11/18 08:47 AM (7 years, 6 days ago) |
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just thought I'd update on this one...
it's fucking Blobtown Arizona!

I thought that the Aspergillus was spreading from under the salt, and whacked more on, but it looks liek it could be bruising from having the water sucked out?

either way it's a mess

and it's growing slowly as it's in a spot that is generally about 60 degrees f
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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Re: Knots or Trich? [Re: keeno] 1
#24905133 - 01/11/18 09:50 AM (7 years, 6 days ago) |
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Interesting to say the least.
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keeno
enthusiast



Registered: 06/01/11
Posts: 2,696
Loc: UK
Last seen: 14 days, 16 hours
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it's mutant city alright!
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RockyB
A little more than newb



Registered: 11/30/17
Posts: 301
Last seen: 4 years, 2 months
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Re: Knots or Trich? [Re: keeno] 1
#24905639 - 01/11/18 02:37 PM (7 years, 6 days ago) |
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It's pretty cool if nothing else. It's nice to know even with the contamination and low temps it's still producing.
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Ziran
The Hero of Time




Registered: 02/03/16
Posts: 6,034
Loc: Temple of Time
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Re: Knots or Trich? [Re: RockyB] 1
#24905785 - 01/11/18 03:57 PM (7 years, 6 days ago) |
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Mutants!
-------------------- Song Of Healing
Updated Pf Tek Guide
Ziran's Teks
AMU Q&A Thread
The Chinese word for nature is zìrán and it means that of which is of itself.

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keeno
enthusiast



Registered: 06/01/11
Posts: 2,696
Loc: UK
Last seen: 14 days, 16 hours
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Re: Knots or Trich? [Re: RockyB]
#24906037 - 01/11/18 05:29 PM (7 years, 6 days ago) |
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Quote:
RockyB said: It's pretty cool if nothing else. It's nice to know even with the contamination and low temps it's still producing.
well kinda.... that fat one is about 5 days old and still just as big as the last knuckle on my thumb... I think the rest of the blobs will join up and murder me in my sleep before I have anythin I can harvest
-------------------- Check out my Psilocybe Cyanofriscosa (Allenii) TEK
Top fungi-knowlodgy with THE TRIBE!
THE TRIBE
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RockyB
A little more than newb



Registered: 11/30/17
Posts: 301
Last seen: 4 years, 2 months
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Re: Knots or Trich? [Re: keeno]
#24907544 - 01/12/18 09:53 AM (7 years, 5 days ago) |
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Quote:
keeno said:
Quote:
RockyB said: It's pretty cool if nothing else. It's nice to know even with the contamination and low temps it's still producing.
well kinda.... that fat one is about 5 days old and still just as big as the last knuckle on my thumb... I think the rest of the blobs will join up and murder me in my sleep before I have anythin I can harvest 
Hahahaha
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Skizor1337
Trip King



Registered: 06/19/12
Posts: 1,120
Loc: USA
Last seen: 2 months, 5 days
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Re: Knots or Trich? [Re: keeno]
#24907954 - 01/12/18 12:50 PM (7 years, 5 days ago) |
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Quote:
keeno said:
Quote:
RockyB said: It's pretty cool if nothing else. It's nice to know even with the contamination and low temps it's still producing.
well kinda.... that fat one is about 5 days old and still just as big as the last knuckle on my thumb... I think the rest of the blobs will join up and murder me in my sleep before I have anythin I can harvest 
Why haven't you tossed that yet?
-------------------- 100% Professional Psychonaut
Trippin' Aint Easy
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keeno
enthusiast



Registered: 06/01/11
Posts: 2,696
Loc: UK
Last seen: 14 days, 16 hours
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curiosity
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