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Offlinekeeno
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Knots or Trich?
    #24891488 - 01/05/18 09:29 AM (7 years, 12 days ago)

howdy folks, happy Friday to you :highfive:

I've gotten the fear. This looks like trouble....


what do you think?

should I launch into orbit and nuke this one from space?  :biggestnuke:

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OfflineSkizor1337
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Re: Knots or Trich? [Re: keeno] * 1
    #24891500 - 01/05/18 09:36 AM (7 years, 12 days ago)

Quote:

keeno said:
howdy folks, happy Friday to you :highfive:

I've gotten the fear. This looks like trouble....


what do you think?

should I launch into orbit and nuke this one from space?  :biggestnuke:




I am not a contam expert by any means but this could be contam

Here's my recent thread with very similar results. I had trouble taking pics but maybe you can reference it to see if it matches or not

https://www.shroomery.org/forums/showflat.php/Number/24881915


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Re: Knots or Trich? [Re: Skizor1337] * 1
    #24891527 - 01/05/18 09:58 AM (7 years, 12 days ago)

Looks like trich in it's early stages. I'd nuke it from orbit. Wait for a TC to confirm though.

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Re: Knots or Trich? [Re: Skizor1337]
    #24891570 - 01/05/18 10:26 AM (7 years, 12 days ago)

looks pretty close...
did you see it turn green before you tossed it?

I've had a few folks suggesting that it's forming blobs and not trich... I'll report back with my findings

cheers Skizor :cookiemonster:

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Re: Knots or Trich? [Re: keeno] * 1
    #24891572 - 01/05/18 10:27 AM (7 years, 12 days ago)

Looks like some ugly blob action right now. sometimes you'll get that from over misting and wet substrate

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Re: Knots or Trich? [Re: bodhisatta]
    #24891594 - 01/05/18 10:37 AM (7 years, 12 days ago)

ah that's interesting, cheers Bod
It's my 2nd mono and I've been trying to recreate comebackkids surface conditions, but I may well have over misted.
cheers for your thoughts!  :billymaythumbup:

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OfflineFascistbullyboy
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Re: Knots or Trich? [Re: keeno] * 1
    #24891631 - 01/05/18 11:01 AM (7 years, 12 days ago)

It ain't trich that's for sure.

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Re: Knots or Trich? [Re: Fascistbullyboy]
    #24891758 - 01/05/18 12:09 PM (7 years, 12 days ago)

good news, cheers boss :highfive:

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OfflineSkizor1337
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Re: Knots or Trich? [Re: keeno] * 1
    #24891997 - 01/05/18 02:32 PM (7 years, 12 days ago)

Quote:

keeno said:
good news, cheers boss :highfive:




Yea dude I tossed it.

I would also toss yours. The one guy said that letting mold in your space will contaminate future projects.

I can't take that chance.


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Re: Knots or Trich? [Re: Skizor1337] * 1
    #24892006 - 01/05/18 02:38 PM (7 years, 12 days ago)

If trich substrates fucked a room me and a lot of others would be having a bunch of issues. Its poor form to keep moldy substrate around but its not like it fucks you

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OfflineSkizor1337
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Re: Knots or Trich? [Re: bodhisatta]
    #24892024 - 01/05/18 02:46 PM (7 years, 12 days ago)

Quote:

bodhisatta said:
If trich substrates fucked a room me and a lot of others would be having a bunch of issues. Its poor form to keep moldy substrate around but its not like it fucks you





I used bleach to clean ceiling to floor haha. I may have went overkill then.

It's all good


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Re: Knots or Trich? [Re: Skizor1337]
    #24892042 - 01/05/18 02:53 PM (7 years, 12 days ago)

How’s it looking now mate intrested to see how it gets on! Hope it’s all good

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OfflineFascistbullyboy
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Re: Knots or Trich? [Re: Mitchell1992]
    #24892117 - 01/05/18 03:28 PM (7 years, 12 days ago)



This trich before sporulating

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Re: Knots or Trich? [Re: Fascistbullyboy]
    #24892147 - 01/05/18 03:42 PM (7 years, 12 days ago)


About to go green


Not trich


Also not trich

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OfflineFascistbullyboy
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Re: Knots or Trich? [Re: bodhisatta] * 1
    #24892159 - 01/05/18 03:47 PM (7 years, 12 days ago)

Keeno didn't you say in another post the other day that you was trying that moisture Tek(can't remember who you said posted it but he got that kid with chocolate on his face for an avatar)?

Then quoting bod in another post they could be blobs due to being too wet. And I'm sure bod will agree.

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Re: Knots or Trich? [Re: Fascistbullyboy] * 1
    #24892179 - 01/05/18 03:55 PM (7 years, 12 days ago)

That is knot trich.


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Re: Knots or Trich? [Re: Ziran] * 2
    #24892187 - 01/05/18 04:02 PM (7 years, 12 days ago)

I see what you did there :lol:

Quote:

Ziran said:
That is knot trich.




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Re: Knots or Trich? [Re: Tormato] * 1
    #24892323 - 01/05/18 04:52 PM (7 years, 12 days ago)

Keeping mold around is not a great thing at all...no ot does not fuck you but having a clean area can be the difference between making past your first couple flushes.
Substates get weak after fruiting so a high sporeload with a weak sub will often mean contams will win...vacuum your floors once in awhile


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Re: Knots or Trich? [Re: bodhisatta] * 1
    #24892374 - 01/05/18 05:10 PM (7 years, 12 days ago)

Quote:

bodhisatta said:
If trich substrates fucked a room me and a lot of others would be having a bunch of issues. Its poor form to keep moldy substrate around but its not like it fucks you



I second this :whathesaid:

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Re: Knots or Trich? [Re: cronicr] * 1
    #24892386 - 01/05/18 05:14 PM (7 years, 12 days ago)

Quote:

cronicr said:
Keeping mold around is not a great thing at all...no ot does not fuck you but having a clean area can be the difference between making past your first couple flushes.
Substates get weak after fruiting so a high sporeload with a weak sub will often mean contams will win...vacuum your floors once in awhile




:whathesaid:

Pretty fair to say any precautions taken will increase your chances of future success


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Re: Knots or Trich? [Re: Tormato]
    #24893584 - 01/06/18 07:26 AM (7 years, 11 days ago)

cheers for the input everyone!

any whilst the blobs haven't gone green, this has...



so it's gonna have to go out.
shit
shame it too cold for it to do its thing out there
:stanhopefacepalm:

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OfflineFascistbullyboy
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Re: Knots or Trich? [Re: keeno] * 1
    #24893593 - 01/06/18 07:31 AM (7 years, 11 days ago)

Shame. I bet I've got green somewhere it doesn't feel right if I ain't lol.

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Re: Knots or Trich? [Re: Fascistbullyboy] * 1
    #24893606 - 01/06/18 07:38 AM (7 years, 11 days ago)

Ah man what a bummer just wasn’t meant to be! Better luck next time

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Re: Knots or Trich? [Re: Mitchell1992] * 1
    #24893622 - 01/06/18 07:48 AM (7 years, 11 days ago)

Dammit...
:youcaughtme:


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Re: Knots or Trich? [Re: Tormato]
    #24893698 - 01/06/18 08:52 AM (7 years, 11 days ago)

cheers folks! I'm gonna move it out of my grow area and stick a bit of salt paste over the trich, see if I can't coax a flush...

but them's the breaks eh? :nojustno:

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Re: Knots or Trich? [Re: keeno]
    #24893708 - 01/06/18 08:58 AM (7 years, 11 days ago)

You sure that's not bruising? Or better, why are you sure it's not bruising?

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Re: Knots or Trich? [Re: RockyB]
    #24893715 - 01/06/18 09:05 AM (7 years, 11 days ago)

the colour's wrong IMO
too green, not blue enough

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Re: Knots or Trich? [Re: keeno]
    #24893720 - 01/06/18 09:08 AM (7 years, 11 days ago)

Huh, it looks like early bruising to me. Before it goes dark enough to look blue. I had something similar and I freaked out, and someone said before you do anything take a cue tip and swipe it. If you have green residue it's bad, if not it's most likely bruising.

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Re: Knots or Trich? [Re: RockyB] * 1
    #24893727 - 01/06/18 09:13 AM (7 years, 11 days ago)

Thats clearly not bruising lol.

Its probably aspergillus and not trich. A microscope would confirm.
Trich usually has telltale white trich mycelium before the green shows. Aspergillus usually shows up like that from my experience

The qtip test is ridiculous. Mold grows on things. Bruising is a color change of mycelium. You can clearly tell its mold growing and not just a color change. Besides sometimes blue bruised mycelium makes the qtip blue and noobs get all freaked.

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Re: Knots or Trich? [Re: bodhisatta]
    #24893774 - 01/06/18 09:38 AM (7 years, 11 days ago)

Quote:

bodhisatta said:
Thats clearly not bruising lol.

Its probably aspergillus and not trich. A microscope would confirm.
Trich usually has telltale white trich mycelium before the green shows. Aspergillus usually shows up like that from my experience

The qtip test is ridiculous. Mold grows on things. Bruising is a color change of mycelium. You can clearly tell its mold growing and not just a color change. Besides sometimes blue bruised mycelium makes the qtip blue and noobs get all freaked.




Dang, I'm just trying to understand all this, so I can understand some false positives, but do you ever have a false negative? Maybe I'm just dumb, but I can't seem to see the difference in characteristics.

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Re: Knots or Trich? [Re: RockyB] * 1
    #24893799 - 01/06/18 09:54 AM (7 years, 11 days ago)

trich

notice the bruising around the trich. zoom in and inspect the grainy texture

asparagus

nothing really grainy just green and gross looking. like the picture posted a few jawns up.

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Re: Knots or Trich? [Re: mushboy] * 1
    #24893804 - 01/06/18 09:56 AM (7 years, 11 days ago)

I think your asparagus is bad with aspergillus

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Re: Knots or Trich? [Re: bodhisatta] * 1
    #24893813 - 01/06/18 10:01 AM (7 years, 11 days ago)

:strokebeard2: maybe..

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Re: Knots or Trich? [Re: bodhisatta]
    #24893818 - 01/06/18 10:07 AM (7 years, 11 days ago)

That jade/emerald green that aspergillus and trich produce is pretty hard to confuse with bruising IMO. When I harvest using scissors or knife the stumps usually turn dark green, but even then it´s pretty distinct from the aspergillus green.


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Re: Knots or Trich? [Re: Mateja]
    #24893824 - 01/06/18 10:11 AM (7 years, 11 days ago)

ive noticed almost every lgt grow ive done bruises greenish blue.


bruising is a reaction. molds are just fucking molds.

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Re: Knots or Trich? [Re: bodhisatta]
    #24893830 - 01/06/18 10:13 AM (7 years, 11 days ago)

Oopsy! It happens :lol:

Quote:

bodhisatta said:
I think your asparagus is bad with aspergillus




:lamastare: :yeahthatsfunny:


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Re: Knots or Trich? [Re: Tormato]
    #24893993 - 01/06/18 11:44 AM (7 years, 11 days ago)

and is aspergillus to be treated the same as trich?

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Re: Knots or Trich? [Re: keeno] * 1
    #24894001 - 01/06/18 11:47 AM (7 years, 11 days ago)

i toss when i see it. my house is a loaded with aspergillus. it grows on juice cups left out, agar plates if im not careful. bread molds with it like right away if left out.

them bitches run deep.

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Re: Knots or Trich? [Re: keeno] * 1
    #24894003 - 01/06/18 11:48 AM (7 years, 11 days ago)

If ya mean toss...yes. Cant remember if their spores are actuallysmaller then most filters?


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Re: Knots or Trich? [Re: keeno] * 1
    #24894004 - 01/06/18 11:48 AM (7 years, 11 days ago)

Assuming it is actually aspergillus its still a toss situation. Aspergillus should theoretically be easier to salt tho. Say you put it somewhere outside to salvage rather than burying it.

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Re: Knots or Trich? [Re: cronicr] * 1
    #24894032 - 01/06/18 12:02 PM (7 years, 11 days ago)

Quote:

cronicr said:
If ya mean toss...yes. Cant remember if their spores are actuallysmaller then most filters?




2-3.5 microns according to the net...I’m going with yes they are smaller than most commonly used filters in this hobby


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Re: Knots or Trich? [Re: Tormato] * 1
    #24894045 - 01/06/18 12:08 PM (7 years, 11 days ago)

Thsnks:super:


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Re: Knots or Trich? [Re: cronicr] * 1
    #24894051 - 01/06/18 12:13 PM (7 years, 11 days ago)

You bet! :thumbup:


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Re: Knots or Trich? [Re: Tormato]
    #24894137 - 01/06/18 01:00 PM (7 years, 11 days ago)

shiiit. SFDs are only 3 microns.


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Re: Knots or Trich? [Re: Ziran] * 1
    #24894142 - 01/06/18 01:02 PM (7 years, 11 days ago)

Quote:

Ziran said:
shiiit. SFDs are only 3 microns.




Yerp...And I've read that Tyvex can vary between 2-15 microns :undecided:


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Re: Knots or Trich? [Re: Tormato] * 1
    #24894153 - 01/06/18 01:08 PM (7 years, 11 days ago)

Well. Im glad I use unmodded lids for the most part


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Re: Knots or Trich? [Re: Ziran]
    #24894248 - 01/06/18 01:58 PM (7 years, 11 days ago)

Did you know in the hepa filters you use for flow hoods the average gap between fibers is way larger than 0.3 microns

Quote:


The air space between HEPA filter fibres is typically much greater than 0.3 μm. The common assumption that a HEPA filter acts like a sieve where particles smaller than the largest opening can pass through is incorrect and impractical




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Re: Knots or Trich? [Re: bodhisatta]
    #24894275 - 01/06/18 02:11 PM (7 years, 11 days ago)

Nope. Sure didnt. Ive also never used one XD


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Re: Knots or Trich? [Re: Ziran]
    #24894283 - 01/06/18 02:14 PM (7 years, 11 days ago)

I remember reading that not to long ago, right now I have 6mm hole in the center of the lid very tightly stuffed with polyfil and 4 layers of micropore tape, 2 on each side of the lid. The tape is of course tightly taped on pressing down the poly even further. This seems much more safe than just polyfil. And much more practical especially when it comes to shaking jars. I know this is not ideal but it´s the best I have at the moment.


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Edited by Mateja (01/06/18 02:15 PM)

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Re: Knots or Trich? [Re: Mateja]
    #24894293 - 01/06/18 02:17 PM (7 years, 11 days ago)

Also I will try unmodded lids next. Ziran, do you keep the lid on loose during colonization? I don´t men to jack the thread sry, you can shoot me a PM Ziran when you have the time.


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Edited by Mateja (01/06/18 02:18 PM)

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Re: Knots or Trich? [Re: Mateja]
    #24894299 - 01/06/18 02:19 PM (7 years, 11 days ago)

Quote:

Mateah said:
Also I will try unmodded lids next. Ziran, do you keep the lid on loose during colonization? I don´t men to jack the thread sry, you can shoot me a PM Ziran when you have the time.




Yes. also loosen & foil when pcing.


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Re: Knots or Trich? [Re: bodhisatta]
    #24894317 - 01/06/18 02:26 PM (7 years, 11 days ago)

Quote:

bodhisatta said:
Did you know in the hepa filters you use for flow hoods the average gap between fibers is way larger than 0.3 microns

Quote:


The air space between HEPA filter fibres is typically much greater than 0.3 μm. The common assumption that a HEPA filter acts like a sieve where particles smaller than the largest opening can pass through is incorrect and impractical








Do you know of anyone who has success with UV sterilizers?  Seems like one could just run one all the time in one''s workspace to keep viable airborn contams to a minimum but I dunno, maybe spores are too resilient to be inactivated that way.


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Re: Knots or Trich? [Re: ballsalsa]
    #24894362 - 01/06/18 02:41 PM (7 years, 11 days ago)

Quote:

Mateah said:
Micropore tape
Quote:

stonesun said:
Well I didn't measure the the pore sizes,
instead took a piece of tape and mounted it on a slide.
Hydrated some P. cubensis spores and with an inoculation loop
mounted them on the tape.
The gaps are huge comparing to cubensis spores, which are ~12-17µm.
I circled the fibers with individual spores sticked to them.
Sorry for the kinda crappy images, didn't put a whole lotta effort in it...

100X


400X


400X





Quote:

RogerRabbit said:
Quote:

sandman420 said:
This is from a faq about 3M Micropore tape, from 3M themselves.
from 3m

Quote:

we don't claim that the tapes filter or block microorganisms.





Quote:

Question:
Is Micropore tape sterile? Can it be sterilized?
Answer:
3M Surgical tapes are sold clean, not sterile.





seems like micropore tape is a shitty choice for mushroom cultivation.






Remember, there's how a product performs, and there's what the manufacturers lawyers make them say about how it performs to prevent liability.

Micropore tape works pretty good to cover the holes on brf jars, but of course after sterilization, you couldn't take the tape off and use it again.  That's why they say it can't be autoclaved.  Also, if the pores are small enough to filter bacteria, then they're small enough for our use.RR




Quote:

lipa said:
I use 2-3 layers of micropore tape on a 1/8-1/4 inch hole on my lids for grain spawn. I have no issues with contaminates unless I am careless. I go through thousands of jars a year and only buy a new box maybe twice a year. A total of 24 bucks for 24 rolls. I think it works pretty good.

Lipa




https://www.shroomery.org/forums/showflat.php/Number/13643478#13643478




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Re: Knots or Trich? [Re: bodhisatta]
    #24896238 - 01/07/18 11:40 AM (7 years, 10 days ago)

Quote:

bodhisatta said:
Assuming it is actually aspergillus its still a toss situation. Aspergillus should theoretically be easier to salt tho. Say you put it somewhere outside to salvage rather than burying it.




yeah I salted it and tucked away somewhere outside, just to see how it develops. these blobs are fascinating. :cookiemonster:

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Re: Knots or Trich? [Re: keeno]
    #24905025 - 01/11/18 08:47 AM (7 years, 6 days ago)

just thought I'd update on this one...

it's fucking Blobtown Arizona!


I thought that the Aspergillus was spreading from under the salt, and whacked more on, but it looks liek it could be bruising from having the water sucked out?



either way it's a mess



and it's growing slowly as it's in a spot that is generally about 60 degrees f

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Re: Knots or Trich? [Re: keeno] * 1
    #24905133 - 01/11/18 09:50 AM (7 years, 6 days ago)

Interesting to say the least.

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Re: Knots or Trich? [Re: bodhisatta]
    #24905147 - 01/11/18 09:58 AM (7 years, 6 days ago)

it's mutant city alright! :laugh:

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Re: Knots or Trich? [Re: keeno] * 1
    #24905639 - 01/11/18 02:37 PM (7 years, 6 days ago)

It's pretty cool if nothing else. It's nice to know even with the contamination and low temps it's still producing.

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Re: Knots or Trich? [Re: RockyB] * 1
    #24905785 - 01/11/18 03:57 PM (7 years, 6 days ago)

Mutants!


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Re: Knots or Trich? [Re: RockyB]
    #24906037 - 01/11/18 05:29 PM (7 years, 6 days ago)

Quote:

RockyB said:
It's pretty cool if nothing else. It's nice to know even with the contamination and low temps it's still producing.





well kinda....
that fat one is about 5 days old and still just as big as the last knuckle on my thumb...
I think the rest of the blobs will join up and murder me in my sleep before I have anythin I can harvest :laugh:  :zombie3:


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Re: Knots or Trich? [Re: keeno]
    #24907544 - 01/12/18 09:53 AM (7 years, 5 days ago)

Quote:

keeno said:
Quote:

RockyB said:
It's pretty cool if nothing else. It's nice to know even with the contamination and low temps it's still producing.





well kinda....
that fat one is about 5 days old and still just as big as the last knuckle on my thumb...
I think the rest of the blobs will join up and murder me in my sleep before I have anythin I can harvest :laugh:  :zombie3:




Hahahaha

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Re: Knots or Trich? [Re: keeno]
    #24907954 - 01/12/18 12:50 PM (7 years, 5 days ago)

Quote:

keeno said:
Quote:

RockyB said:
It's pretty cool if nothing else. It's nice to know even with the contamination and low temps it's still producing.





well kinda....
that fat one is about 5 days old and still just as big as the last knuckle on my thumb...
I think the rest of the blobs will join up and murder me in my sleep before I have anythin I can harvest :laugh:  :zombie3:





Why haven't you tossed that yet?


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Re: Knots or Trich? [Re: Skizor1337]
    #24910094 - 01/13/18 08:53 AM (7 years, 4 days ago)

curiosity

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