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SerpeantKing
Stranger
Registered: 11/06/17
Posts: 18
Last seen: 5 years, 6 months
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First PF TEK Interesting results!!!
#24887757 - 01/03/18 04:03 PM (7 years, 14 days ago) |
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Need some help guys If anyone might have run into this problem before and has a solution. Spores came from a sponsor(B+, Golden Teacher, PF classic was older and a gift from The Works)
I followed the PF Tek to a T and was very meticulous about sterilization, substrate, and sterile inoculation(I even used a disposable rain coat).
I followed a lot of WillyMyco's advice from Youtube if you know about him when it came to avoiding contamination.
Thus far contamination hasn't been a problem but I have run into one major one.
The Jars were inoculated three weeks ago, on the 12th of December. 7 PF classics 9 Golden Teachers and 8 B+'s
The jars are left to sit in a sterile plastic shoebox(with no lid). Jars have to be hidden for a couple days each week. Lids for the shoeboxes have holes(much like a terrarium) when I do move them. The location I hide them in is colder(once alarmed to see the temp had gone down to 60f!!!!!!!)
Otherwise, the jars have sat at 73-80f.(I've brought the temp down to 73f after noticing faster growth when placing them in a colder enviroment). Light is provided through a window off in the corner giving ambient light. Light is on a winter solstice time schedule here in N.Y.
Out of 24 jars, 18 have germinated and are colonizing aggressively on at least 1,2,3 inoculation points.
7 B+'s 5 Golden Teachers 5 PF classics
B+ has been the very best. The fastest to colonize(falling shortly behind Golden Teacher) and the MOST CONSISTENT to show germination. The Golden Teachers came out fast and aggressive, but only 5 out of 9 jars. Pf Classics were slow(none are at 100% but were more consistent than Golden Teacher.
It's really amazing to see success in my first grow without contaminents but I am still sad-facing here. Out of all strains, I was most interested in Golden Teacher because of it's potency and other qualities that have apparently given them the name "Golden Teachers" and only 5 jars out of 9. It's been three weeks and most say there's a problem at two. I have seen more and more of these jars germinate, some taking longer than others. Is it possible these jars still have a chance to germinate, especially since I dropped the temp????
I did make a few mistakes along the way other than the hot temp. I was so paranoid about contaminates that I was paranoid about taking the foil lids off. I wanted to touch them as little as possible but after slow growth and seeking advice from you guys, I was advised to remove the lids. Growth really took off after I did that. I'll be removing the lids right after inoculation on future grows. I also used paper tape and inoculated straight through the paper tape. After getting frustrated with slower growth near 100%(taking a long time to fill up the bottom) I removed the tape. They grew faster and quickly reached 100%. Owing it to the removal of the tape, I inspected all of the jars, even the ones with the tape still on them and their growth took off too. It was the change in temp that made a difference. Has anyone ran into a problem like this? Is there any good reason why mycellium would grow in some areas and not others? I did shake the syringe well(spores were difficult to break up). The Golden Teachers were shooken up the most until I was satisfied the entire mass broke apart.
With the uncolonized jars, at the inoculation points, there are crystally looking lines spreading from the point, but no fuzzy mycellium. I'm wondering if it's also possible I didn't get the edges(I just injected and looked for wet substrate until I stopped) and the Mycellium is growing deep within the substrate. Has anyone ran into issues similar to this?
Edited by SerpeantKing (01/06/18 09:55 PM)
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SadTurkey
ayy


Registered: 10/21/17
Posts: 410
Loc: Netherlands
Last seen: 1 year, 4 months
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Re: First PF TEK Interesting results!!! [Re: SerpeantKing]
#24887992 - 01/03/18 05:49 PM (7 years, 14 days ago) |
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I'd just wait a bit more, multispore is a mixed bag of genetics so colonization speed can vary greatly. It's also possible the mycelium is growing somewhere out of sight like you said.
By the way, all varieties of cubensis are pretty much the same in terms of potency (except Penis Envy) so don't sweat it too much if some of your Golden Teachers turn out to be bunk. They sure are pretty though!
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theoldclarysage
Wanderer



Registered: 03/26/17
Posts: 279
Last seen: 10 hours, 43 minutes
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Re: First PF TEK Interesting results!!! [Re: SadTurkey]
#24888020 - 01/03/18 05:58 PM (7 years, 14 days ago) |
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Remove the vendors name please.
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SerpeantKing
Stranger
Registered: 11/06/17
Posts: 18
Last seen: 5 years, 6 months
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I went ahead and did that (theoldclarysage)-sorry about that.
Some of the Golden Teachers that fully colonized and one B+ grew pins overnight so I birthed them.
Was really interesting to birth these 'things'. I say 'things' because it's so strange to hold and be one with something so "alien" and yet so alive. I can sense the power and life coming off of it.
All of them had a nice Mushroom smell that reminds me of freshly picked edible mushrooms. No mold growing through the verm layer or weird discolored areas.
I didn't expect it but I had a bit of a hard time dunking and rolling these these things since I only had four. I had to put them in seperate pots but they really wrestled with me on staying afloat. Things didn't go over gently. One side of the lid I'd use to hold them down would fall over, including the plate I used. Either that or it would just crush them against the bottom of the jar. I used cold tap water. After trying a few different situations, I finally got them to sink in measuring cup containers with a bowl and a jar sitting on top. It looks like one cake might have a damaged spot from trying to keep it under. No one mentioned to me these things fight to stay afloat!
In any case, I hope I didn't damage the mycellium too bad. More of the B+ are pinning and reaching 100%. I plan on keeping them at room temp for 24 hours then putting them in a SGFC. Here are some pics of the fully colonized cakes...

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Shrooms4life
TrippyShaman



Registered: 06/08/17
Posts: 1,376
Loc: Under the Viel
Last seen: 30 days, 15 hours
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Re: First PF TEK Interesting results!!! [Re: SerpeantKing]
#24895565 - 01/07/18 12:38 AM (7 years, 10 days ago) |
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They are pinning in the jars id dunk and roll and put into fruiting now.
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SerpeantKing
Stranger
Registered: 11/06/17
Posts: 18
Last seen: 5 years, 6 months
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Re: First PF TEK Interesting results!!! [Re: Shrooms4life]
#24898901 - 01/08/18 04:11 PM (7 years, 9 days ago) |
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Yeah I went ahead and did so. The one thing I noticed after their 24 hour dunk is a difference in smell. A moldy, or should I say "mildewy" smell now permeates from them. Still smells like Mushrooms, just not as dry and fresh, a very heavy mildewy smell(is this normal after dunking?).
The cakes are also noticably heavier. They no longer have the light "rice cake" feeling but are heavier now, like wet soil(water absorbtion). Other people think the cakes smell like mushrooms and I'm just being paranoid. I hope I am.
Next problem is the casing. The only verm I have is this really fine verm. It's powdery and after doing the dunk and roll, they look like breaded chicken. Some of the Vermiculite may have been excessive and fell off the cakes onto the foil. Should I recase them more carefully? My house has central heat and I think the air humidity in the house is pretty dry. After waking today, I checked on them and noticed that it was completely dry. The Perlite was dry like sand. I made sure to sterilize the casing verm by putting in the oven like the Psilocyibin Mushroom Bible suggested. Made sure it was cool before I used it. Because of my limited supply of perlite, I had to use an even smaller terrarium that has a non latching lid.(Good thing I prepared two SGFC's). It just sits on the top and probably provides just a little additional airflow. I spray the sides of the FC with sterlized water(boiling the water in a pot for about 15-30 minutes) and filtered with a Britta filter. Their sitting in a clean, unused room with the rest of the jars that are still colonizing. Light is provided through indirect sunlight(through the short equinox sun winter time) and I also just leave the overhead lights on in the room for 12/12. I hope the combination is sufficient. It's the best with what I can do with limited supplies and funds.
I spray the inside of the terrarium, making sure to only hit the walls or perlite when I see there's no more droplets on the side, followed by fanning. I spray about 20 times until I see water collect on the sides of the bin, then I am satisfied and fan. No standing water at the bottom. I'm thinking the mildewy heavy mushroom smell is possibly just built up carbon-dioxide as it disappears after fanning.
The pins that were on the cake, are still there, but their covered in Verm(it couldn't be helped) and I'm hoping to see them grow and sprout right out of the casing layer but I have noticed little growth in the past day. They've only been in there for less than a day now. I'll be making room for another B+ fully colonized that is showing one little pin.
Some Teks say I should birth them as soon as I see pins. Some Teks say I should let the pins grow invitro a little more, to encourage more before birthing.
What's you guy's opinion on the fine verm casing layer, the smell, and pinning invitro?
The jars are forming knots before full colonization and forming pins within a few days after reaching %100(I figured they would be fine to birth after seeing pins). My personal rule on birthing at this point is either 7 days after the outside is fully colonized or 100% colonization plus pins(whichever comes first), is that the way to do it?
One more thing, some Teks also advice scraping off the verm layer with a fork(even if it means tearing some Mycellium) while other Tek's claim that dumping the top layer and rinsing off what you can is the way to go. I didn't want to mess around with a fork so I went with the ladder option.
Oh and the cake that got damaged(had a little hole pierced in it) is "self healing". It's forming wispy bands of mycellium over the hole-"plugging it".
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