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Sally Hatchet



Registered: 12/22/17
Posts: 175
Loc: Hole in the ground
Last seen: 4 years, 10 months
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inoculated one of four holes - PF tek
#24878404 - 12/30/17 10:09 AM (7 years, 18 days ago) |
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I ran out of spore solution and ended up only having enough for MAYBE 1/4cc-1/3cc left by the time I got to my last jar. I was only able to inoculate one of the 4 holes from the jar lid.
Is this enough to colonize the jar? If it slowly grows is there a higher chance for it to stall out?
Thank you!
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Re: inoculated one of four holes - PF tek [Re: Sally Hatchet] 1
#24878479 - 12/30/17 10:52 AM (7 years, 18 days ago) |
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It might stall, it might be fine. Btw you can get away with only a few drops per hole, the 1/4 cc was dreamed up by spore vendors looking to sell product. A single syringe can do far more than 10 jars.
Hell you could sterilize a quart of water, inject your whole syringe into it. Then aspirate dozens of syringes from it. That's how you stretch a spore syringe.
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Cakewonder
Cake Mastah!



Registered: 12/14/17
Posts: 1,389
Loc: North
Last seen: 14 hours, 10 minutes
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Re: inoculated one of four holes - PF tek [Re: Pastywhyte]
#24878523 - 12/30/17 11:16 AM (7 years, 18 days ago) |
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-------------------- The PF Tek is my area.
www.zamnesia.nl
RustyWhyte is Commercial!
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Sally Hatchet



Registered: 12/22/17
Posts: 175
Loc: Hole in the ground
Last seen: 4 years, 10 months
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Re: inoculated one of four holes - PF tek [Re: Cakewonder]
#24878765 - 12/30/17 01:09 PM (7 years, 18 days ago) |
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Thanks for the replies! I’ll have to remember that for my next spore solution.
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Shrooms4life
TrippyShaman



Registered: 06/08/17
Posts: 1,376
Loc: Under the Viel
Last seen: 30 days, 18 hours
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Re: inoculated one of four holes - PF tek [Re: Pastywhyte]
#24879273 - 12/30/17 06:05 PM (7 years, 18 days ago) |
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Quote:
Pastywhyte said: It might stall, it might be fine. Btw you can get away with only a few drops per hole, the 1/4 cc was dreamed up by spore vendors looking to sell product. A single syringe can do far more than 10 jars.
Hell you could sterilize a quart of water, inject your whole syringe into it. Then aspirate dozens of syringes from it. That's how you stretch a spore syringe.
Shit dude that's a great idea, how long could courts of spore solution stay viable for?
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hamloaf
Q-dood ®©™√



Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 5 hours, 45 minutes
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Re: inoculated one of four holes - PF tek [Re: Shrooms4life]
#24879277 - 12/30/17 06:08 PM (7 years, 18 days ago) |
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Depends on how you store them.
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Sally Hatchet



Registered: 12/22/17
Posts: 175
Loc: Hole in the ground
Last seen: 4 years, 10 months
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Re: inoculated one of four holes - PF tek [Re: hamloaf]
#24879685 - 12/30/17 09:31 PM (7 years, 18 days ago) |
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Quote:
hamloaf said: Depends on how you store them.
In a dark drawer, typically 75 degrees maybe dropping to 70.
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hamloaf
Q-dood ®©™√



Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 5 hours, 45 minutes
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Re: inoculated one of four holes - PF tek [Re: Sally Hatchet]
#24879728 - 12/30/17 09:41 PM (7 years, 18 days ago) |
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They'll last longer stored in the fridge.
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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: inoculated one of four holes - PF tek [Re: Sally Hatchet]
#24879781 - 12/30/17 09:47 PM (7 years, 18 days ago) |
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Quote:
Sally Hatchet said: Thanks for the replies! I’ll have to remember that for my next spore solution.
You don´t have to wait for more syringes to squeeze out a few more spores, just aspirate sterile water in to the empty syringe and you will be able to inoculate a few more cakes. But maybe use more than a drop this time cause it will be much more diluted. GL
-------------------- Cakes inside Water Tub
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