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OfflineSally Hatchet
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Registered: 12/22/17
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inoculated one of four holes - PF tek
    #24878404 - 12/30/17 10:09 AM (7 years, 18 days ago)

I ran out of spore solution and ended up only having enough for MAYBE 1/4cc-1/3cc left by the time I got to my last jar. I was only able to inoculate one of the 4 holes from the jar lid.

Is this enough to colonize the jar?
If it slowly grows is there a higher chance for it to stall out?

Thank you!


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InvisiblePastywhyteMDiscord
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Re: inoculated one of four holes - PF tek [Re: Sally Hatchet] * 1
    #24878479 - 12/30/17 10:52 AM (7 years, 18 days ago)

It might stall, it might be fine. Btw you can get away with only a few drops per hole, the 1/4 cc was dreamed up by spore vendors looking to sell product. A single syringe can do far more than 10 jars.

Hell you could sterilize a quart of water, inject your whole syringe into it. Then aspirate dozens of syringes from it. That's how you stretch a spore syringe.

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OfflineCakewonder
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Re: inoculated one of four holes - PF tek [Re: Pastywhyte]
    #24878523 - 12/30/17 11:16 AM (7 years, 18 days ago)

:hellyeah:


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OfflineSally Hatchet
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Re: inoculated one of four holes - PF tek [Re: Cakewonder]
    #24878765 - 12/30/17 01:09 PM (7 years, 18 days ago)

Thanks for the replies!
I’ll have to remember that for my next spore solution.


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OfflineShrooms4life
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Re: inoculated one of four holes - PF tek [Re: Pastywhyte]
    #24879273 - 12/30/17 06:05 PM (7 years, 18 days ago)

Quote:

Pastywhyte said:
It might stall, it might be fine. Btw you can get away with only a few drops per hole, the 1/4 cc was dreamed up by spore vendors looking to sell product. A single syringe can do far more than 10 jars.

Hell you could sterilize a quart of water, inject your whole syringe into it. Then aspirate dozens of syringes from it. That's how you stretch a spore syringe.




Shit dude that's a great idea, how long could courts of spore solution stay viable for?

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OfflinehamloafM
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Re: inoculated one of four holes - PF tek [Re: Shrooms4life]
    #24879277 - 12/30/17 06:08 PM (7 years, 18 days ago)

Depends on how you store them.


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How I Get Stuff done. - My Reference Guide. - My Grows.

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OfflineSally Hatchet
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Registered: 12/22/17
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Re: inoculated one of four holes - PF tek [Re: hamloaf]
    #24879685 - 12/30/17 09:31 PM (7 years, 18 days ago)

Quote:

hamloaf said:
Depends on how you store them.





In a dark drawer, typically 75 degrees maybe dropping to 70.


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OfflinehamloafM
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Re: inoculated one of four holes - PF tek [Re: Sally Hatchet]
    #24879728 - 12/30/17 09:41 PM (7 years, 18 days ago)

They'll last longer stored in the fridge.


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How I Get Stuff done. - My Reference Guide. - My Grows.

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InvisibleMateja
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Registered: 07/14/16
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Re: inoculated one of four holes - PF tek [Re: Sally Hatchet]
    #24879781 - 12/30/17 09:47 PM (7 years, 18 days ago)

Quote:

Sally Hatchet said:
Thanks for the replies!
I’ll have to remember that for my next spore solution.



You don´t have to wait for more syringes to squeeze out a few more spores, just aspirate sterile water in to the empty syringe and you will be able to inoculate a few more cakes. But maybe use more than a drop this time cause it will be much more diluted. GL


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