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OfflineDiggler
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Does cloning from a pin then make a monculture?
    #24870796 - 12/27/17 04:53 AM (3 years, 4 months ago)

Sorry for my ignorance in genetics. I'm gearing up for my first agar from syringe this week....and looking ahead, I was just wondering if when you grow a pin in a dish, then transfer that to another agar dish, is what's produced a monoculture?


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Invisiblenube424
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Re: Does cloning from a pin then make a monculture? [Re: Diggler]
    #24870804 - 12/27/17 05:15 AM (3 years, 4 months ago)

Well thats a clone. If u grow a pin out on agar u could still get sectoring so i dont think its a monoculture till u transfer a few times and the sectoring stops. But i could be wrong.


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Invisiblehamloaf
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Re: Does cloning from a pin then make a monculture? [Re: Diggler]
    #24871127 - 12/27/17 11:43 AM (3 years, 4 months ago)

Quote:

Diggler said:
Sorry for my ignorance in genetics. I'm gearing up for my first agar from syringe this week....and looking ahead, I was just wondering if when you grow a pin in a dish, then transfer that to another agar dish, is what's produced a monoculture?



No.


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InvisiblebodhisattaM
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Re: Does cloning from a pin then make a monculture? [Re: Diggler]
    #24871136 - 12/27/17 11:47 AM (3 years, 4 months ago)

Quote:

Diggler said:
Sorry for my ignorance in genetics. I'm gearing up for my first agar from syringe this week....and looking ahead, I was just wondering if when you grow a pin in a dish, then transfer that to another agar dish, is what's produced a monoculture?



Nope, its a clone culture,
Sometimes clones are monocultures but more often than not on MS substrates they're a culture of more than a few strains working together.


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Re: Does cloning from a pin then make a monculture? [Re: Diggler]
    #24871137 - 12/27/17 11:48 AM (3 years, 4 months ago)

Repeat those last two steps about 50 times...but with ms.  Not a clone.


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Invisiblehamloaf
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Re: Does cloning from a pin then make a monculture? [Re: bodhisatta]
    #24871187 - 12/27/17 12:16 PM (3 years, 4 months ago)

Quote:

bodhisatta said:
Quote:

Diggler said:
Sorry for my ignorance in genetics. I'm gearing up for my first agar from syringe this week....and looking ahead, I was just wondering if when you grow a pin in a dish, then transfer that to another agar dish, is what's produced a monoculture?



Nope, its a clone culture,
Sometimes clones are monocultures.



That's true, but it's very rare.


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OfflineCrombie
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Re: Does cloning from a pin then make a monculture? [Re: mushboy]
    #24871219 - 12/27/17 12:29 PM (3 years, 4 months ago)

Quote:

mushboy said:
Repeat those last two steps about 50 times...but with ms.  Not a clone.




Your quote confused me. Is it not possible to get a monoculture transferring from a clone?


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Invisiblehamloaf
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Re: Does cloning from a pin then make a monculture? [Re: Crombie]
    #24871226 - 12/27/17 12:31 PM (3 years, 4 months ago)

It's possible.  He's saying you're going to have to transfer it about 50 times in order to generate a mono from a multi spore culture.


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OfflineCrombie
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Re: Does cloning from a pin then make a monculture? [Re: hamloaf]
    #24871228 - 12/27/17 12:33 PM (3 years, 4 months ago)

Ooooh, I misread his original post then. I don't have a clone yet to try on, but are there usually a limited number of strains when you put a clone to agar?

Edit: Poorly worded at first, but hamloaf still answered


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Edited by Crombie (12/27/17 01:15 PM)


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Invisiblehamloaf
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Re: Does cloning from a pin then make a monculture? [Re: Crombie]
    #24871244 - 12/27/17 12:37 PM (3 years, 4 months ago)

A clone culture will have a limited number of strains.  The strains become fewer the more times said clone culture is transferred from.


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OfflineDiggler
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Re: Does cloning from a pin then make a monculture? [Re: hamloaf]
    #24875547 - 12/29/17 09:13 AM (3 years, 4 months ago)

Ok thanks for the responses!


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