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Offlinespace_shrooms


Registered: 05/04/17
Posts: 438
Last seen: 10 months, 4 days
Is this metabolites?
    #24870720 - 12/27/17 12:34 AM (7 years, 22 days ago)

These are my first grain jars. Is there something wrong with the middle one? It's gotten a bit juicy and it's hard to tell, but I think the liquid is slightly yellow. I'm guessing metabolites, but would appreciate the opinion of knowledgable others. They've been fully colonised for a few days and in a hot room (about 85F) which may be the problem. I've moved them to somewhere a bit cooler since.

I know I'll get a good indication when I open them and have a smell in a few days, but as an impatient noob, I'm asking for opinions on what's going on here. Thanks  :bandana:




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OfflineWeavieWonder
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Registered: 08/18/16
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Re: Is this metabolites? [Re: space_shrooms]
    #24871238 - 12/27/17 10:35 AM (7 years, 21 days ago)

Bacteria.  The middle jar looks fucked, but the other two might be spawnable.  Wouldn't recommend for g2g.  Give em a wiff before using.  Anything that smells like sour milk or dirty gym socks should be discarded.  Normal room temp for colonization is more than adequate.  Excessive temps stress the myc and favor bacterial growth.

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Offlinespace_shrooms


Registered: 05/04/17
Posts: 438
Last seen: 10 months, 4 days
Re: Is this metabolites? [Re: WeavieWonder]
    #24871575 - 12/27/17 01:30 PM (7 years, 21 days ago)

True - alright thanks. I'm hoping you're wrong about the other two because I did a g2g with a same looking jar from the same batch 5 days ago - 1 jar to another 6, and they are coming along well - almost done. Neither of them or the g2g ones have turned juicy and yellowish like the middle one above.

Does bacteria and/or metabolites always happen when jars get too warm? I mean, are bacteria always present in grain jars and will become an issue if the environment is wrong (e.g. too hot or too much liquid), or are they generally a result of contam due to unsatisfactory process? Wondering if what is at fault here is my sterile process or the environment (or both).

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OfflineWeavieWonder
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Registered: 08/18/16
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Re: Is this metabolites? [Re: space_shrooms]
    #24871781 - 12/27/17 03:47 PM (7 years, 21 days ago)

"sterilized" grains are never 100% bacteria free.  Providing ideal conditions for the mushroom myc is the key to ensuring the remaining bacteria do not recover, and proliferate before 100% colonization is reached.  Excess moisture and heat will expodite bacterial growth every time.  It's common for bacteria to be introduced at inoculation also.  Spore syringes are notorious for this.  Inexperienced agar users have a tough time too as bacteria can very good at hiding.

With that said, I do not use grain spawn for G2G unless I'm 101% positive it's clean.  If it's questionable in the slightest, I'll spawn it to bulk instead.  A little bacteria is ok for spawning bulk sub, but even a little bacteria can turn into a shit show quickly in a g2g.  Use your best judgement, g2g if you wish.  Sometimes people get lucky.

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Offlinespace_shrooms


Registered: 05/04/17
Posts: 438
Last seen: 10 months, 4 days
Re: Is this metabolites? [Re: WeavieWonder]
    #24872089 - 12/27/17 06:09 PM (7 years, 21 days ago)

Thanks for taking the time to explain that  :cheers:

These grains were started with agar. I had a dirty syringe with obvious bacteria on the first plate and was surprised to see it still appear on the second plate after I transferred what I thought was a  clean piece. The wedges I used for the jars  were from the third transfer and well grown out and looking good, but you're probably right and there might have been some bacteria hiding. Now I know to do more transfers to be 100% sure.

I've actually got a good feeling about all the other jars - the g2g ones look very healthy and I can't believe how quickly they're colonising - I think they'll be 100% in under a week.

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OfflineBojerk Horse Man
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Registered: 12/14/17
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Last seen: 6 years, 8 months
Re: Is this metabolites? [Re: WeavieWonder]
    #24872134 - 12/27/17 06:23 PM (7 years, 21 days ago)

Great explanation and clarification.  I wish everyone on this board could write like this.  :rockon:

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OfflineWeavieWonder
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Re: Is this metabolites? [Re: space_shrooms]
    #24872172 - 12/27/17 06:37 PM (7 years, 21 days ago)

Quote:

aicaca said:

I've actually got a good feeling about all the other jars - the g2g ones look very healthy and I can't believe how quickly they're colonising - I think they'll be 100% in under a week.




Sounds encouraging, but practice cautious optimism.  A lot of times contaminants will not make their presence be known until close to full colonization.  Not trying to be a debby downer, just stating facts.  I hope you are successful.:smile: 

Quote:

aicaca said:
Now I know to do more transfers to be 100% sure.




:thumbup:  It takes me an average of 10 transfers from spore to achieve a culture that is organized/free of contams.

Edited by WeavieWonder (12/27/17 06:40 PM)

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