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Offlinepsilocyanide
Terry-Flappinsworth
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Multiple questions (myc. recovery/generations/LC)
    #24864036 - 12/23/17 02:20 PM (7 years, 25 days ago)

I have a few questions, and figured it would be better to ask them all at once instead of posting a bunch of new threads.

1) Can I use my rye grain soak water to make lc if it has coffee and gypsum according to RR's method (half water, half coffee)

2) Why can mycelium be broken up constantly, when agitating a LC without needing to recover.  LC does better with a magnetic stirrer constantly moving, but shaking a jar takes 2 days to even see mycelium again.

3) What qualifies a generation?  Is it only if it is grown out to a fruit and cloned from the fruit, or would one (Grain/LC/Agar)2(Grain/LC/Agar) transfer count as gaining a generation?

4) If you use some liquid culture on a jar to test it, how long should you wait after seeing healthy mycelium grow in the jar to know that its not contaminated?

Thanks in advance to anyone that answers any of these.


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:minigun:                  \\:freewilly:\\

Edited by psilocyanide (12/23/17 02:37 PM)

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InvisiblebodhisattaMDiscordReddit
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Re: Multiple questions (myc. recovery/generations/LC) [Re: psilocyanide]
    #24864121 - 12/23/17 03:04 PM (7 years, 25 days ago)

1. Yes. Shouldn't be a problem sterilized. Try it out and let us Know though.

2. I think you're seeing the same things but it never "disappears" in the LC after a "shake"

3. Spores to fruit to spores is a gen. Other expansion can be called a generation but i would call them expansions.

4. Ya know when you know

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Offlinepsilocyanide
Terry-Flappinsworth
I'm a teapot User Gallery

Registered: 06/20/12
Posts: 235
Last seen: 6 years, 3 months
Re: Multiple questions (myc. recovery/generations/LC) [Re: bodhisatta]
    #24864172 - 12/23/17 03:26 PM (7 years, 25 days ago)

for 2: Ive read shaking a jar twice slows it down more than helps because the myc. takes time to recover, but an LC can be constantly stirred, why doesnt it need to recover when in a LC? 

Thanks for answers.


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