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InvisibleTFI
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Registered: 04/06/14
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Agar confusion
    #24863308 - 12/23/17 06:55 AM (7 years, 25 days ago)

Hello all, recently made four grocery tek agar dishes with poly fill lids (have used this method for awhile with no problems). I used 2-3 year old syringes kept in my food refrigerator. Despite that i saw good growth previous times (approx 6 months ago). But this morning looking at plates that are on their  third transfer i noticed one plate with a green/brown blob so i set him aside, my other two rhyismorphic plates has white specks that look like the start of growth when you spore syringe a new agar plate, i had small amounts of liquid sitting atop my plates when transfered so maybe my agar wedges colonized the liquid and its growing new? Also i have just enough good looking agar growth to make a cut for new plates, should i cut new wedges?

White speck growth is to the side where my unlevel shelf ran the liquid to but agar wedge is going strong in middle. Not sure if i should scrap whole project an order new syringes or if i should cut the good parts now... Also whats the ideal location to cut a wedge from.. Closer to original wedge piece or outer rim of growth? Thanks and sorry for the qumb questions

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OfflinehamloafM
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Re: Agar confusion [Re: TFI]
    #24863317 - 12/23/17 07:09 AM (7 years, 25 days ago)

Up to you. 
Outer rim of growth.


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OfflineFeedYourMind
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Re: Agar confusion [Re: hamloaf]
    #24863397 - 12/23/17 08:31 AM (7 years, 25 days ago)

If you are that concerned with how your agar plates are done, I would go ahead and transfer to a new plate to ease your own worries. Especially if you are aiming for an isolate.


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OfflinePsilicon
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Re: Agar confusion [Re: hamloaf]
    #24863398 - 12/23/17 08:32 AM (7 years, 25 days ago)

I wouldn't order new syringes.  If you have trouble with your solution running on your plates, try putting a single drop of solution on a sterile cotton swab and giving your agar just a tiny, gentle swipe.

If I have too much condensation a couple days after making my no-pour plates, I'll just carefully dump it in the bottom of my SAB.


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Agar - what, why and how?  Everything a beginner needs to know.
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InvisibleTFI
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Re: Agar confusion [Re: Psilicon]
    #24863649 - 12/23/17 11:22 AM (7 years, 25 days ago)

Well the sitting liquid was i suppose condensation that slowly ran to one side after it touched wedge, looks like the start of new growth, hopefully not cobweb or some other bs.

Another question, i dont have fund to buy a proper flow hood so i use a sab. But can i run a hepa air purifier in room hours prior to working in said room or am i just kickin up dust.

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Invisible36fuckin5
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Re: Agar confusion [Re: TFI]
    #24864279 - 12/23/17 04:16 PM (7 years, 25 days ago)

Quote:

TFI said:
Well the sitting liquid was i suppose condensation that slowly ran to one side after it touched wedge, looks like the start of new growth, hopefully not cobweb or some other bs.

Another question, i dont have fund to buy a proper flow hood so i use a sab. But can i run a hepa air purifier in room hours prior to working in said room or am i just kickin up dust.




It won't help.


--------------------
Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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InvisibleTFI
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Re: Agar confusion [Re: 36fuckin5]
    #24864953 - 12/23/17 11:17 PM (7 years, 25 days ago)

Well crap lol

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