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Gav91
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does this look right? casing layer, cambodian cubensis
#24861554 - 12/22/17 07:20 AM (7 years, 26 days ago) |
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Hey this is my first time growin shrooms without a brf tek kit. I got a cambodian cubensis spore syringe, mad an lc and inoculated some buckwheat. Growth was fast and clean. So I transferred to casings 2 days ago. Peat,coffe grounds and some perlite for aeration Just wondering if this looks right?
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hamloaf
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Re: does this look right? casing layer, cambodian cubensis [Re: Gav91]
#24861565 - 12/22/17 07:25 AM (7 years, 26 days ago) |
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Looks contaminated.
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Psilosoulful

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Re: does this look right? casing layer, cambodian cubensis [Re: hamloaf]
#24861624 - 12/22/17 08:09 AM (7 years, 26 days ago) |
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Coffee grounds in a casing?
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Leftfield420
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Re: does this look right? casing layer, cambodian cubensis [Re: hamloaf]
#24861631 - 12/22/17 08:16 AM (7 years, 26 days ago) |
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Quote:
hamloaf said: Looks contaminated.
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TheMadHatter420
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Re: does this look right? casing layer, cambodian cubensis [Re: Leftfield420]
#24861639 - 12/22/17 08:21 AM (7 years, 26 days ago) |
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Coffee grounds in the casing will encourage mold to grow. Spores to LC is a HUGE gamble that usually doesn't pay off. I agree that looks contaminated.
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Edited by TheMadHatter420 (12/22/17 08:22 AM)
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Gav91
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Re: does this look right? casing layer, cambodian cubensis [Re: TheMadHatter420]
#24861721 - 12/22/17 09:14 AM (7 years, 26 days ago) |
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Dam! Cheers lads, back to the drawing board
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TheMadHatter420
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Re: does this look right? casing layer, cambodian cubensis [Re: Gav91]
#24861728 - 12/22/17 09:16 AM (7 years, 26 days ago) |
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Let it go unless you see mold spores forming. I think it is just bacterial, cause I have ad tubs that looked like that, and I got some fruits from it, then the green mold showed up.
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Gav91
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Re: does this look right? casing layer, cambodian cubensis [Re: TheMadHatter420]
#24861754 - 12/22/17 09:38 AM (7 years, 26 days ago) |
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I stuck em in the fc to see what happens. Even if I got some fruits for spores id be happy. Got some agar going too if not, looks clean so far
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keeno
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Re: does this look right? casing layer, cambodian cubensis [Re: Gav91]
#24861764 - 12/22/17 09:42 AM (7 years, 26 days ago) |
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best of luck with this. the perlite is a waste of time though, that's not aerating anything
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Leftfield420
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Re: does this look right? casing layer, cambodian cubensis [Re: Gav91]
#24861807 - 12/22/17 09:58 AM (7 years, 26 days ago) |
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If that is mold the mold spores will be on your fruits when you print them.... I would NOT print them...
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bodhisatta 
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Re: does this look right? casing layer, cambodian cubensis [Re: Gav91]
#24861827 - 12/22/17 10:13 AM (7 years, 26 days ago) |
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Quote:
Gav91 said: Hey this is my first time growin shrooms without a brf tek kit. I got a cambodian cubensis spore syringe, mad an lc and inoculated some buckwheat. Growth was fast and clean. So I transferred to casings 2 days ago. Peat,coffe grounds and some perlite for aeration Just wondering if this looks right?

That needs to be tossed out yesterday. It's clearly mold lol.
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Gav91
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Re: does this look right? casing layer, cambodian cubensis [Re: keeno]
#24861944 - 12/22/17 11:22 AM (7 years, 26 days ago) |
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Why wouldnt the perlite areate it?
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Gav91
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Re: does this look right? casing layer, cambodian cubensis [Re: Gav91]
#24861957 - 12/22/17 11:27 AM (7 years, 26 days ago) |
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 Does this agar look right? Dont fancy spending 50 quid on new spores :/ sorry bout the shitty phone pic
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Leftfield420
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Re: does this look right? casing layer, cambodian cubensis [Re: Gav91]
#24861964 - 12/22/17 11:31 AM (7 years, 26 days ago) |
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no, agar should appear smooth... That looks like it didn't set up right...Looks like bacteria growing...transfer myc to another plate soon....
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Gav91
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Re: does this look right? casing layer, cambodian cubensis [Re: Leftfield420]
#24861981 - 12/22/17 11:40 AM (7 years, 26 days ago) |
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I used pastywhites tek, agar, potato flakes and honey. But yea I dont think I cooked it long enough. Is that myc enough to transfer? I made some new agar last night using actual potatoe water rather than flakes, just looks like jello
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Leftfield420
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Re: does this look right? casing layer, cambodian cubensis [Re: Gav91]
#24862217 - 12/22/17 02:11 PM (7 years, 26 days ago) |
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Yeah transfer a small piece of mycelium to the agar you made last night...the sooner the better
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Gav91
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Re: does this look right? casing layer, cambodian cubensis [Re: Leftfield420]
#24862629 - 12/22/17 06:07 PM (7 years, 26 days ago) |
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Here it is now?
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keeno
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Re: does this look right? casing layer, cambodian cubensis [Re: Leftfield420]
#24862631 - 12/22/17 06:09 PM (7 years, 26 days ago) |
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I don't think perlite aerates the coir as it's just lumps of a porous rock. I'd say the coir was aerated enough due to it's course texture but I could be wrong as far as I know it's better used to create a humid environment in a Fruiting Chamber as it's got a massive effective surface are that the water can evaporate off of.
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Gav91
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Re: does this look right? casing layer, cambodian cubensis [Re: Leftfield420]
#24862638 - 12/22/17 06:12 PM (7 years, 26 days ago) |
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Quote:
Leftfield420 said: Yeah transfer a small piece of mycelium to the agar you made last night...the sooner the better
I transferred that to the new agar. Ill post pics in this thread when theres some growth
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Gav91
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Re: does this look right? casing layer, cambodian cubensis [Re: keeno]
#24862651 - 12/22/17 06:19 PM (7 years, 26 days ago) |
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Quote:
keeno said: I don't think perlite aerates the coir as it's just lumps of a porous rock. I'd say the coir was aerated enough due to it's course texture but I could be wrong as far as I know it's better used to create a humid environment in a Fruiting Chamber as it's got a massive effective surface are that the water can evaporate off of.
Honestly your prob right. I just use it to encourage root growth in my plants. Logic led me too it... lol I have a bed of wet perlite in the fc, under the tipperware the casings in for humidity too... Tho if everyone on here is right im prob just cultivating mold
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Leftfield420
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Re: does this look right? casing layer, cambodian cubensis [Re: Gav91]
#24862727 - 12/22/17 06:58 PM (7 years, 26 days ago) |
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Keep an eue on those bright white spots...I'm almost positive that looks like trich...if I'm right it will be green within the next day or two at most
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keeno
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Re: does this look right? casing layer, cambodian cubensis [Re: Leftfield420]
#24863140 - 12/23/17 02:25 AM (7 years, 26 days ago) |
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I think that's the perlite there Leftfield 
@OP all good mate, no harm in trying. I usually chuck something into the mix to see if it helps. Fingers crossed it's myc and not mold
Edited by keeno (12/23/17 02:26 AM)
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Leftfield420
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Re: does this look right? casing layer, cambodian cubensis [Re: keeno]
#24863284 - 12/23/17 06:18 AM (7 years, 25 days ago) |
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I didn't read OP added perilite...hopefully none of your mushies decide to eat any perlite...or you'll be chewing on perilite when you try your fruits OP...perilite should stay in the sgfc, it don't belong mixed in your substrate....you're not growing plants OP
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hamloaf
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Re: does this look right? casing layer, cambodian cubensis [Re: Gav91]
#24863323 - 12/23/17 07:13 AM (7 years, 25 days ago) |
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Quote:
Gav91 said:
Here it is now?
Quote:
Gav91 said:
Here it is now?
That's mold, brother. It'll be green in the next day or so.
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Leftfield420
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Re: does this look right? casing layer, cambodian cubensis [Re: hamloaf]
#24863353 - 12/23/17 07:47 AM (7 years, 25 days ago) |
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I thought it looked like trich.... Hamloaf with the confermation...OP I'd trash that and start over...
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bodhisatta 
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Re: does this look right? casing layer, cambodian cubensis [Re: Leftfield420]
#24863412 - 12/23/17 08:45 AM (7 years, 25 days ago) |
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Its mold but it aint trich and aint turning green
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hamloaf
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Re: does this look right? casing layer, cambodian cubensis [Re: bodhisatta]
#24863434 - 12/23/17 09:05 AM (7 years, 25 days ago) |
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It will soon be green.
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Gav91
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Re: does this look right? casing layer, cambodian cubensis [Re: hamloaf]
#24863459 - 12/23/17 09:18 AM (7 years, 25 days ago) |
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Binned! That shit got nasty quick.
Pretty sure my lc is contaminated too, did some on agar and the white growth is green in the center :/ any way to clean it up??
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hamloaf
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Re: does this look right? casing layer, cambodian cubensis [Re: Gav91]
#24863468 - 12/23/17 09:22 AM (7 years, 25 days ago) |
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Put your spores to agar instead of LC or grains, and make several transfers to ensure a clean culture. Once a clean culture on agar has been established the sky's the limit. Meaning the medium of your choice can be inoculated from there as far as LC, or grains are concerned.
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bodhisatta 
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Re: does this look right? casing layer, cambodian cubensis [Re: bodhisatta]
#24863470 - 12/23/17 09:24 AM (7 years, 25 days ago) |
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Quote:
bodhisatta said:
Quote:
Gav91 said: Hey this is my first time growin shrooms without a brf tek kit. I got a cambodian cubensis spore syringe, mad an lc and inoculated some buckwheat. Growth was fast and clean. So I transferred to casings 2 days ago. Peat,coffe grounds and some perlite for aeration Just wondering if this looks right?

That needs to be tossed out yesterday. It's clearly mold lol.

Ain't a green mold. And if for some reason it turns green it's not trichoderma morphology. It would be more likely to be aspergillus. Trich turns green well before the white patches of its mold get that big
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Gav91
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Re: does this look right? casing layer, cambodian cubensis [Re: hamloaf]
#24863476 - 12/23/17 09:27 AM (7 years, 25 days ago) |
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Quote:
hamloaf said: Put your spores to agar instead of LC or grains, and make several transfers to ensure a clean culture. Once a clean culture on agar has been established the sky's the limit. Meaning the medium of your choice can be inoculated from there as far as LC, or grains are concerned.
Can I clean it up using lc to agar? I used the last of my spore syringe to make the lc :/
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bodhisatta 
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Re: does this look right? casing layer, cambodian cubensis [Re: Gav91]
#24863481 - 12/23/17 09:29 AM (7 years, 25 days ago) |
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Ouch, never make a LC directly from spores.
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hamloaf
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Re: does this look right? casing layer, cambodian cubensis [Re: Gav91]
#24863483 - 12/23/17 09:30 AM (7 years, 25 days ago) |
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Quote:
Gav91 said:
Quote:
hamloaf said: Put your spores to agar instead of LC or grains, and make several transfers to ensure a clean culture. Once a clean culture on agar has been established the sky's the limit. Meaning the medium of your choice can be inoculated from there as far as LC, or grains are concerned.
Can I clean it up using lc to agar? I used the last of my spore syringe to make the lc :/
You could try but I don't think so. With liquid mediums once an invader culture establishes it's self it over runs the culture to the point of their NOT being any mushroom myclium in there. Consider ordering a fresh print or spore solution syringe.
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bodhisatta 
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Re: does this look right? casing layer, cambodian cubensis [Re: hamloaf]
#24863500 - 12/23/17 09:38 AM (7 years, 25 days ago) |
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Unless the contamination has a kill factor for cubensis it's not completely removing our intended fungi. It just becomes impossible to separate them with any normal methods.
If you worked in a real lab and that LC was the very last stock of cubensis mycelium they would probably try heating it or cooling it to a temperature at which the contamination cant handle but the intended culture can. But thats entirely unrealistic for at home
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Gav91
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Re: does this look right? casing layer, cambodian cubensis [Re: bodhisatta]
#24863552 - 12/23/17 10:18 AM (7 years, 25 days ago) |
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So I need a fresh spore syringe? Can I message ya pics of the agar ive got and see what ya reckon?
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