Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Liquid Cultures   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   MagicBag.co All-In-One Bags That Don't Suck   Original Sensible Seeds Bulk Cannabis Seeds   Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Bulk Substrate   Left Coast Kratom Buy Kratom Extract   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work

Jump to first unread post Pages: 1 | 2  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineGav91
Stranger
Registered: 09/20/16
Posts: 21
Last seen: 4 years, 3 months
does this look right? casing layer, cambodian cubensis
    #24861554 - 12/22/17 07:20 AM (7 years, 26 days ago)

Hey this is my first time growin shrooms without a brf tek kit.
I got a cambodian cubensis spore syringe, mad an lc and inoculated some buckwheat.
Growth was fast and clean.
So I transferred to casings 2 days ago. Peat,coffe grounds and some perlite for aeration
Just wondering if this looks right?


Extras: Filter Print Post Top
OfflinehamloafM
Q-dood ®©™√
Male User Gallery


Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 8 hours, 38 minutes
Trusted Cultivator
OG Cultivator
Re: does this look right? casing layer, cambodian cubensis [Re: Gav91]
    #24861565 - 12/22/17 07:25 AM (7 years, 26 days ago)

Looks contaminated.


--------------------
     
How I Get Stuff done. - My Reference Guide. - My Grows.

Extras: Filter Print Post Top
OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 4 months, 23 days
Re: does this look right? casing layer, cambodian cubensis [Re: hamloaf]
    #24861624 - 12/22/17 08:09 AM (7 years, 26 days ago)

Coffee grounds in a casing? :what:

Extras: Filter Print Post Top
InvisibleLeftfield420
bong toker
Male


Registered: 02/26/16
Posts: 10,482
Re: does this look right? casing layer, cambodian cubensis [Re: hamloaf]
    #24861631 - 12/22/17 08:16 AM (7 years, 26 days ago)

Quote:

hamloaf said:
Looks contaminated.




:whathesaid:

Extras: Filter Print Post Top
InvisibleTheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,943
Re: does this look right? casing layer, cambodian cubensis [Re: Leftfield420]
    #24861639 - 12/22/17 08:21 AM (7 years, 26 days ago)

Coffee grounds in the casing will encourage mold to grow.
Spores to LC is a HUGE gamble that usually doesn't pay off.
I agree that looks contaminated.


--------------------
JOIN THE POW WOW

Edited by TheMadHatter420 (12/22/17 08:22 AM)

Extras: Filter Print Post Top
OfflineGav91
Stranger
Registered: 09/20/16
Posts: 21
Last seen: 4 years, 3 months
Re: does this look right? casing layer, cambodian cubensis [Re: TheMadHatter420]
    #24861721 - 12/22/17 09:14 AM (7 years, 26 days ago)

Dam! Cheers lads, back to the drawing board

Extras: Filter Print Post Top
InvisibleTheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,943
Re: does this look right? casing layer, cambodian cubensis [Re: Gav91]
    #24861728 - 12/22/17 09:16 AM (7 years, 26 days ago)

Let it go unless you see mold spores forming. I think it is just bacterial, cause I have ad tubs that looked like that, and I got some fruits from it, then the green mold showed up.


--------------------
JOIN THE POW WOW

Extras: Filter Print Post Top
OfflineGav91
Stranger
Registered: 09/20/16
Posts: 21
Last seen: 4 years, 3 months
Re: does this look right? casing layer, cambodian cubensis [Re: TheMadHatter420]
    #24861754 - 12/22/17 09:38 AM (7 years, 26 days ago)

I stuck em in the fc to see what happens. Even if I got some fruits for spores id be happy. Got some agar going too if not, looks clean so far

Extras: Filter Print Post Top
Offlinekeeno
enthusiast
Male User Gallery


Registered: 06/01/11
Posts: 2,696
Loc: UK Flag
Last seen: 14 days, 22 hours
Re: does this look right? casing layer, cambodian cubensis [Re: Gav91]
    #24861764 - 12/22/17 09:42 AM (7 years, 26 days ago)

best of luck with this. the perlite is a waste of time though, that's not aerating anything

Extras: Filter Print Post Top
InvisibleLeftfield420
bong toker
Male


Registered: 02/26/16
Posts: 10,482
Re: does this look right? casing layer, cambodian cubensis [Re: Gav91]
    #24861807 - 12/22/17 09:58 AM (7 years, 26 days ago)

If that is mold the mold spores will be on your fruits when you print them.... I would NOT print them...

Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,915
Loc: Milky way
Trusted Cultivator
Re: does this look right? casing layer, cambodian cubensis [Re: Gav91]
    #24861827 - 12/22/17 10:13 AM (7 years, 26 days ago)

Quote:

Gav91 said:
Hey this is my first time growin shrooms without a brf tek kit.
I got a cambodian cubensis spore syringe, mad an lc and inoculated some buckwheat.
Growth was fast and clean.
So I transferred to casings 2 days ago. Peat,coffe grounds and some perlite for aeration
Just wondering if this looks right?





That needs to be tossed out yesterday. It's clearly mold lol.


Extras: Filter Print Post Top
OfflineGav91
Stranger
Registered: 09/20/16
Posts: 21
Last seen: 4 years, 3 months
Re: does this look right? casing layer, cambodian cubensis [Re: keeno]
    #24861944 - 12/22/17 11:22 AM (7 years, 26 days ago)

Why wouldnt the perlite areate it?

Extras: Filter Print Post Top
OfflineGav91
Stranger
Registered: 09/20/16
Posts: 21
Last seen: 4 years, 3 months
Re: does this look right? casing layer, cambodian cubensis [Re: Gav91]
    #24861957 - 12/22/17 11:27 AM (7 years, 26 days ago)


Does this agar look right? Dont fancy spending 50 quid on new spores :/ sorry bout the shitty phone pic

Extras: Filter Print Post Top
InvisibleLeftfield420
bong toker
Male


Registered: 02/26/16
Posts: 10,482
Re: does this look right? casing layer, cambodian cubensis [Re: Gav91]
    #24861964 - 12/22/17 11:31 AM (7 years, 26 days ago)

no, agar should appear smooth... That looks like it didn't set up right...Looks like bacteria growing...transfer myc to another plate soon....

Extras: Filter Print Post Top
OfflineGav91
Stranger
Registered: 09/20/16
Posts: 21
Last seen: 4 years, 3 months
Re: does this look right? casing layer, cambodian cubensis [Re: Leftfield420]
    #24861981 - 12/22/17 11:40 AM (7 years, 26 days ago)

I used pastywhites tek, agar, potato flakes and honey. But yea I dont think I cooked it long enough.
Is that myc enough to transfer? I made some new agar last night using actual potatoe water rather than flakes, just looks like jello

Extras: Filter Print Post Top
InvisibleLeftfield420
bong toker
Male


Registered: 02/26/16
Posts: 10,482
Re: does this look right? casing layer, cambodian cubensis [Re: Gav91]
    #24862217 - 12/22/17 02:11 PM (7 years, 26 days ago)

Yeah transfer a small piece of mycelium to the agar you made last night...the sooner the better

Extras: Filter Print Post Top
OfflineGav91
Stranger
Registered: 09/20/16
Posts: 21
Last seen: 4 years, 3 months
Re: does this look right? casing layer, cambodian cubensis [Re: Leftfield420]
    #24862629 - 12/22/17 06:07 PM (7 years, 26 days ago)

Here it is now?

Extras: Filter Print Post Top
Offlinekeeno
enthusiast
Male User Gallery


Registered: 06/01/11
Posts: 2,696
Loc: UK Flag
Last seen: 14 days, 22 hours
Re: does this look right? casing layer, cambodian cubensis [Re: Leftfield420]
    #24862631 - 12/22/17 06:09 PM (7 years, 26 days ago)

I don't think perlite aerates the coir as it's just lumps of a porous rock. I'd say the coir was aerated enough due to it's course texture but I could be wrong
as far as I know it's better used to create a humid environment in a Fruiting Chamber as it's got a massive effective surface are that the water can evaporate off of.

Extras: Filter Print Post Top
OfflineGav91
Stranger
Registered: 09/20/16
Posts: 21
Last seen: 4 years, 3 months
Re: does this look right? casing layer, cambodian cubensis [Re: Leftfield420]
    #24862638 - 12/22/17 06:12 PM (7 years, 26 days ago)

Quote:

Leftfield420 said:
Yeah transfer a small piece of mycelium to the agar you made last night...the sooner the better



I transferred that to the new agar. Ill post pics in this thread when theres some growth

Extras: Filter Print Post Top
OfflineGav91
Stranger
Registered: 09/20/16
Posts: 21
Last seen: 4 years, 3 months
Re: does this look right? casing layer, cambodian cubensis [Re: keeno]
    #24862651 - 12/22/17 06:19 PM (7 years, 26 days ago)

Quote:

keeno said:
I don't think perlite aerates the coir as it's just lumps of a porous rock. I'd say the coir was aerated enough due to it's course texture but I could be wrong
as far as I know it's better used to create a humid environment in a Fruiting Chamber as it's got a massive effective surface are that the water can evaporate off of.




Honestly your prob right. I just use it to encourage root growth in my plants. Logic led me too it... lol
I have a bed of wet perlite in the fc, under the tipperware the casings in for humidity too...
Tho if everyone on here is right im prob just cultivating mold :frown:

Extras: Filter Print Post Top
InvisibleLeftfield420
bong toker
Male


Registered: 02/26/16
Posts: 10,482
Re: does this look right? casing layer, cambodian cubensis [Re: Gav91]
    #24862727 - 12/22/17 06:58 PM (7 years, 26 days ago)

Keep an eue on those bright white spots...I'm almost positive that looks like trich...if I'm right it will be green within the next day or two at most

Extras: Filter Print Post Top
Offlinekeeno
enthusiast
Male User Gallery


Registered: 06/01/11
Posts: 2,696
Loc: UK Flag
Last seen: 14 days, 22 hours
Re: does this look right? casing layer, cambodian cubensis [Re: Leftfield420]
    #24863140 - 12/23/17 02:25 AM (7 years, 26 days ago)

I think that's the perlite there Leftfield :laugh:

@OP all good mate, no harm in trying. I usually chuck something into the mix to see if it helps. Fingers crossed it's myc and not mold :cookiemonster:

Edited by keeno (12/23/17 02:26 AM)

Extras: Filter Print Post Top
InvisibleLeftfield420
bong toker
Male


Registered: 02/26/16
Posts: 10,482
Re: does this look right? casing layer, cambodian cubensis [Re: keeno]
    #24863284 - 12/23/17 06:18 AM (7 years, 25 days ago)

I didn't read OP added perilite...hopefully none of your mushies decide to eat any perlite...or you'll be chewing on perilite when you try your fruits OP...perilite should stay in the sgfc, it don't belong mixed in your substrate....you're not growing plants OP

Extras: Filter Print Post Top
OfflinehamloafM
Q-dood ®©™√
Male User Gallery


Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 8 hours, 38 minutes
Trusted Cultivator
OG Cultivator
Re: does this look right? casing layer, cambodian cubensis [Re: Gav91]
    #24863323 - 12/23/17 07:13 AM (7 years, 25 days ago)

Quote:

Gav91 said:
Here it is now?



Quote:

Gav91 said:
Here it is now?



That's mold, brother.  It'll be green in the next day or so.


--------------------
     
How I Get Stuff done. - My Reference Guide. - My Grows.

Extras: Filter Print Post Top
InvisibleLeftfield420
bong toker
Male


Registered: 02/26/16
Posts: 10,482
Re: does this look right? casing layer, cambodian cubensis [Re: hamloaf]
    #24863353 - 12/23/17 07:47 AM (7 years, 25 days ago)

I thought it looked like trich.... Hamloaf with the confermation...OP I'd trash that and start over...

Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,915
Loc: Milky way
Trusted Cultivator
Re: does this look right? casing layer, cambodian cubensis [Re: Leftfield420]
    #24863412 - 12/23/17 08:45 AM (7 years, 25 days ago)

Its mold but it aint trich and aint turning green

Extras: Filter Print Post Top
OfflinehamloafM
Q-dood ®©™√
Male User Gallery


Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 8 hours, 38 minutes
Trusted Cultivator
OG Cultivator
Re: does this look right? casing layer, cambodian cubensis [Re: bodhisatta]
    #24863434 - 12/23/17 09:05 AM (7 years, 25 days ago)

It will soon be green.


--------------------
     
How I Get Stuff done. - My Reference Guide. - My Grows.

Extras: Filter Print Post Top
OfflineGav91
Stranger
Registered: 09/20/16
Posts: 21
Last seen: 4 years, 3 months
Re: does this look right? casing layer, cambodian cubensis [Re: hamloaf]
    #24863459 - 12/23/17 09:18 AM (7 years, 25 days ago)

Binned! That shit got nasty quick.

Pretty sure my lc is contaminated too, did some on agar and the white growth is green in the center :/ any way to clean it up??

Extras: Filter Print Post Top
OfflinehamloafM
Q-dood ®©™√
Male User Gallery


Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 8 hours, 38 minutes
Trusted Cultivator
OG Cultivator
Re: does this look right? casing layer, cambodian cubensis [Re: Gav91]
    #24863468 - 12/23/17 09:22 AM (7 years, 25 days ago)

Put your spores to agar instead of LC or grains, and make several transfers to ensure a clean culture.  Once a clean culture on agar has been established the sky's the limit.  Meaning the medium of your choice can be inoculated from there as far as LC, or grains are concerned.


--------------------
     
How I Get Stuff done. - My Reference Guide. - My Grows.

Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,915
Loc: Milky way
Trusted Cultivator
Re: does this look right? casing layer, cambodian cubensis [Re: bodhisatta]
    #24863470 - 12/23/17 09:24 AM (7 years, 25 days ago)

Quote:

bodhisatta said:
Quote:

Gav91 said:
Hey this is my first time growin shrooms without a brf tek kit.
I got a cambodian cubensis spore syringe, mad an lc and inoculated some buckwheat.
Growth was fast and clean.
So I transferred to casings 2 days ago. Peat,coffe grounds and some perlite for aeration
Just wondering if this looks right?





That needs to be tossed out yesterday. It's clearly mold lol.





Ain't a green mold. And if for some reason it turns green it's not trichoderma morphology. It would be more likely to be aspergillus. Trich turns green well before the white patches of its mold get that big

Extras: Filter Print Post Top
OfflineGav91
Stranger
Registered: 09/20/16
Posts: 21
Last seen: 4 years, 3 months
Re: does this look right? casing layer, cambodian cubensis [Re: hamloaf]
    #24863476 - 12/23/17 09:27 AM (7 years, 25 days ago)

Quote:

hamloaf said:
Put your spores to agar instead of LC or grains, and make several transfers to ensure a clean culture.  Once a clean culture on agar has been established the sky's the limit.  Meaning the medium of your choice can be inoculated from there as far as LC, or grains are concerned.



Can I clean it up using lc to agar? I used the last of my spore syringe to make the lc :/

Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,915
Loc: Milky way
Trusted Cultivator
Re: does this look right? casing layer, cambodian cubensis [Re: Gav91]
    #24863481 - 12/23/17 09:29 AM (7 years, 25 days ago)

Ouch, never make a LC directly from spores.

Extras: Filter Print Post Top
OfflinehamloafM
Q-dood ®©™√
Male User Gallery


Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 8 hours, 38 minutes
Trusted Cultivator
OG Cultivator
Re: does this look right? casing layer, cambodian cubensis [Re: Gav91]
    #24863483 - 12/23/17 09:30 AM (7 years, 25 days ago)

Quote:

Gav91 said:
Quote:

hamloaf said:
Put your spores to agar instead of LC or grains, and make several transfers to ensure a clean culture.  Once a clean culture on agar has been established the sky's the limit.  Meaning the medium of your choice can be inoculated from there as far as LC, or grains are concerned.



Can I clean it up using lc to agar? I used the last of my spore syringe to make the lc :/



You could try but I don't think so.  With liquid mediums once an invader culture establishes it's self it over runs the culture to the point of their NOT being any mushroom myclium in there.  Consider ordering a fresh print or spore solution syringe.


--------------------
     
How I Get Stuff done. - My Reference Guide. - My Grows.

Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,915
Loc: Milky way
Trusted Cultivator
Re: does this look right? casing layer, cambodian cubensis [Re: hamloaf]
    #24863500 - 12/23/17 09:38 AM (7 years, 25 days ago)

Unless the contamination has a kill factor for cubensis it's not completely removing our intended fungi. It just becomes impossible to separate them with any normal methods.

If you worked in a real lab and that LC was the very last stock of cubensis mycelium they would probably try heating it or cooling it to a temperature at which the contamination cant handle but the intended culture can. But thats entirely unrealistic for at home

Extras: Filter Print Post Top
OfflineGav91
Stranger
Registered: 09/20/16
Posts: 21
Last seen: 4 years, 3 months
Re: does this look right? casing layer, cambodian cubensis [Re: bodhisatta]
    #24863552 - 12/23/17 10:18 AM (7 years, 25 days ago)

So I need a fresh spore syringe? Can I message ya pics of the agar ive got and see what ya reckon?

Extras: Filter Print Post Top
Jump to top Pages: 1 | 2  [ show all ]

Shop: Mushroom-Hut Liquid Cultures   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   MagicBag.co All-In-One Bags That Don't Suck   Original Sensible Seeds Bulk Cannabis Seeds   Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Bulk Substrate   Left Coast Kratom Buy Kratom Extract   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work


Similar ThreadsPosterViewsRepliesLast post
* bottom casing layer pleezr 1,998 2 01/11/02 09:16 AM
by ar393
* reusing casing layer? 1stimer 2,071 6 10/07/02 04:57 PM
by matts
* Re: Using/Mixing Perlite as a casing layer BaldCuban 2,119 1 05/15/00 06:58 PM
by Anonymous
* Re: Bottom Casing Layer? Poll! oscill8 3,307 9 04/27/01 10:51 AM
by Foe20kid
* Adding perlite to casing layer? CoolTJ 961 1 09/03/02 07:13 AM
by Fd3000
* Cool to have perlite in casing layer? Dogomush 1,187 5 10/31/02 01:31 PM
by Dogomush
* Bottom Casing Layer?? drenaline 1,085 3 10/24/02 03:37 PM
by drenaline
* casing layer drying out ryacentauri 875 2 01/12/03 03:08 AM
by ryacentauri

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,231 topic views. 31 members, 182 guests and 30 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2025 Mind Media. Some rights reserved.

Generated in 0.029 seconds spending 0.008 seconds on 12 queries.