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InvisibleLeftfield420
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Re: does this look right? casing layer, cambodian cubensis [Re: Gav91]
    #24862727 - 12/22/17 06:58 PM (7 years, 26 days ago)

Keep an eue on those bright white spots...I'm almost positive that looks like trich...if I'm right it will be green within the next day or two at most

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Offlinekeeno
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Re: does this look right? casing layer, cambodian cubensis [Re: Leftfield420]
    #24863140 - 12/23/17 02:25 AM (7 years, 26 days ago)

I think that's the perlite there Leftfield :laugh:

@OP all good mate, no harm in trying. I usually chuck something into the mix to see if it helps. Fingers crossed it's myc and not mold :cookiemonster:

Edited by keeno (12/23/17 02:26 AM)

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InvisibleLeftfield420
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Re: does this look right? casing layer, cambodian cubensis [Re: keeno]
    #24863284 - 12/23/17 06:18 AM (7 years, 25 days ago)

I didn't read OP added perilite...hopefully none of your mushies decide to eat any perlite...or you'll be chewing on perilite when you try your fruits OP...perilite should stay in the sgfc, it don't belong mixed in your substrate....you're not growing plants OP

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OfflinehamloafM
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Re: does this look right? casing layer, cambodian cubensis [Re: Gav91]
    #24863323 - 12/23/17 07:13 AM (7 years, 25 days ago)

Quote:

Gav91 said:
Here it is now?



Quote:

Gav91 said:
Here it is now?



That's mold, brother.  It'll be green in the next day or so.


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InvisibleLeftfield420
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Re: does this look right? casing layer, cambodian cubensis [Re: hamloaf]
    #24863353 - 12/23/17 07:47 AM (7 years, 25 days ago)

I thought it looked like trich.... Hamloaf with the confermation...OP I'd trash that and start over...

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InvisiblebodhisattaMDiscordReddit
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Re: does this look right? casing layer, cambodian cubensis [Re: Leftfield420]
    #24863412 - 12/23/17 08:45 AM (7 years, 25 days ago)

Its mold but it aint trich and aint turning green

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OfflinehamloafM
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Re: does this look right? casing layer, cambodian cubensis [Re: bodhisatta]
    #24863434 - 12/23/17 09:05 AM (7 years, 25 days ago)

It will soon be green.


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OfflineGav91
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Re: does this look right? casing layer, cambodian cubensis [Re: hamloaf]
    #24863459 - 12/23/17 09:18 AM (7 years, 25 days ago)

Binned! That shit got nasty quick.

Pretty sure my lc is contaminated too, did some on agar and the white growth is green in the center :/ any way to clean it up??

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OfflinehamloafM
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Re: does this look right? casing layer, cambodian cubensis [Re: Gav91]
    #24863468 - 12/23/17 09:22 AM (7 years, 25 days ago)

Put your spores to agar instead of LC or grains, and make several transfers to ensure a clean culture.  Once a clean culture on agar has been established the sky's the limit.  Meaning the medium of your choice can be inoculated from there as far as LC, or grains are concerned.


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Re: does this look right? casing layer, cambodian cubensis [Re: bodhisatta]
    #24863470 - 12/23/17 09:24 AM (7 years, 25 days ago)

Quote:

bodhisatta said:
Quote:

Gav91 said:
Hey this is my first time growin shrooms without a brf tek kit.
I got a cambodian cubensis spore syringe, mad an lc and inoculated some buckwheat.
Growth was fast and clean.
So I transferred to casings 2 days ago. Peat,coffe grounds and some perlite for aeration
Just wondering if this looks right?





That needs to be tossed out yesterday. It's clearly mold lol.





Ain't a green mold. And if for some reason it turns green it's not trichoderma morphology. It would be more likely to be aspergillus. Trich turns green well before the white patches of its mold get that big

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OfflineGav91
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Re: does this look right? casing layer, cambodian cubensis [Re: hamloaf]
    #24863476 - 12/23/17 09:27 AM (7 years, 25 days ago)

Quote:

hamloaf said:
Put your spores to agar instead of LC or grains, and make several transfers to ensure a clean culture.  Once a clean culture on agar has been established the sky's the limit.  Meaning the medium of your choice can be inoculated from there as far as LC, or grains are concerned.



Can I clean it up using lc to agar? I used the last of my spore syringe to make the lc :/

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Re: does this look right? casing layer, cambodian cubensis [Re: Gav91]
    #24863481 - 12/23/17 09:29 AM (7 years, 25 days ago)

Ouch, never make a LC directly from spores.

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OfflinehamloafM
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Re: does this look right? casing layer, cambodian cubensis [Re: Gav91]
    #24863483 - 12/23/17 09:30 AM (7 years, 25 days ago)

Quote:

Gav91 said:
Quote:

hamloaf said:
Put your spores to agar instead of LC or grains, and make several transfers to ensure a clean culture.  Once a clean culture on agar has been established the sky's the limit.  Meaning the medium of your choice can be inoculated from there as far as LC, or grains are concerned.



Can I clean it up using lc to agar? I used the last of my spore syringe to make the lc :/



You could try but I don't think so.  With liquid mediums once an invader culture establishes it's self it over runs the culture to the point of their NOT being any mushroom myclium in there.  Consider ordering a fresh print or spore solution syringe.


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Re: does this look right? casing layer, cambodian cubensis [Re: hamloaf]
    #24863500 - 12/23/17 09:38 AM (7 years, 25 days ago)

Unless the contamination has a kill factor for cubensis it's not completely removing our intended fungi. It just becomes impossible to separate them with any normal methods.

If you worked in a real lab and that LC was the very last stock of cubensis mycelium they would probably try heating it or cooling it to a temperature at which the contamination cant handle but the intended culture can. But thats entirely unrealistic for at home

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OfflineGav91
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Re: does this look right? casing layer, cambodian cubensis [Re: bodhisatta]
    #24863552 - 12/23/17 10:18 AM (7 years, 25 days ago)

So I need a fresh spore syringe? Can I message ya pics of the agar ive got and see what ya reckon?

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