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OfflineBooooooooom
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Jars not showing growth...
    #24861487 - 12/22/17 08:01 AM (3 years, 4 months ago)

I always DO rye berries soak 6-24h, then boil for 10-15min depending on the soak.
Then I pc for 1hour 15 psi.
Then inoculate 1cc of lc and Keep jars in exactly 24 degrees celcius.
No signs of growth after i open the jars and also No bad smell, like i did not inject.
I tried the same lc on a pf cake and it worked No problems. So i thought the moisture content was of and did the simmer for 45min. Same as before No growth....
I dont know what the problem is..
What the fuck is wrong seriosly!!!??!


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OfflineShrooms4life
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Re: Jars not showing growth... [Re: Booooooooom]
    #24861490 - 12/22/17 08:05 AM (3 years, 4 months ago)

Hmm very weird sounding problem your having, well it doesn't seem to be your LC because it noc'd the pf cake up... have you tried that lc on any other type of grains or wbs?


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OfflineCrombie
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Re: Jars not showing growth... [Re: Booooooooom]
    #24861550 - 12/22/17 09:15 AM (3 years, 4 months ago)

Quote:

Booooooooom said:

No signs of growth after i open the jars






How long did you wait to open them?


--------------------
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OfflineBooooooooom
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Re: Jars not showing growth... [Re: Crombie]
    #24861856 - 12/22/17 12:28 PM (3 years, 4 months ago)

Quote:

Shrooms4life said:
Hmm very weird sounding problem your having, well it doesn't seem to be your LC because it noc'd the pf cake up... have you tried that lc on any other type of grains or wbs?



I have only done rye berries and the pf tek.

Quote:

Crombie said:
Quote:

Booooooooom said:

No signs of growth after i open the jars






How long did you wait to open them?




Maybe 4-5days, some were a weekend old or more with No growth or smell...


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OfflineBig Worm
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Re: Jars not showing growth... [Re: Booooooooom]
    #24861858 - 12/22/17 12:31 PM (3 years, 4 months ago)

You have to PC for at least 90 minutes.  Even longer if you live at a higher elevation.


And you don't want to open your jars during colonization.  You opened it up to contamination.  You smell the substrate through your SFD.


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OfflineShrooms4life
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Re: Jars not showing growth... [Re: Booooooooom]
    #24861864 - 12/22/17 12:33 PM (3 years, 4 months ago)

Quote:

Booooooooom said:
Quote:

Shrooms4life said:
Hmm very weird sounding problem your having, well it doesn't seem to be your LC because it noc'd the pf cake up... have you tried that lc on any other type of grains or wbs?



I have only done rye berries and the pf tek.

Quote:

Crombie said:
Quote:

Booooooooom said:

No signs of growth after i open the jars






How long did you wait to open them?




Maybe 4-5days, some were a weekend old or more with No growth or smell...



That's not a long enough time to really do shit... shoot up your next jars and just leave them alone and if you still don't see shit in like 3 weeks then somthing is up.


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OfflineBooooooooom
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Re: Jars not showing growth... [Re: Shrooms4life]
    #24861898 - 12/22/17 12:55 PM (3 years, 4 months ago)

Quote:

Shrooms4life said:
Quote:

Booooooooom said:
Quote:

Shrooms4life said:
Hmm very weird sounding problem your having, well it doesn't seem to be your LC because it noc'd the pf cake up... have you tried that lc on any other type of grains or wbs?



I have only done rye berries and the pf tek.

Quote:

Crombie said:
Quote:

Booooooooom said:

No signs of growth after i open the jars






How long did you wait to open them?




Maybe 4-5days, some were a weekend old or more with No growth or smell...



That's not a long enough time to really do shit... shoot up your next jars and just leave them alone and if you still don't see shit in like 3 weeks then somthing is up.




Yep already done that, been a couple of days and No growth, il wait 2-3weeks then look again. People usually get finished jars in 10-14 days and i cant belive i need to wait 3weeks to se some signs of growth. In that rate i will wait for months for One jar...


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OfflineBooooooooom
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Re: Jars not showing growth... [Re: Big Worm]
    #24862327 - 12/22/17 05:33 PM (3 years, 4 months ago)

Quote:

Big Worm said:
You have to PC for at least 90 minutes.  Even longer if you live at a higher elevation.


And you don't want to open your jars during colonization.  You opened it up to contamination.  You smell the substrate through your SFD.



Yes thank you. I already know not to open the jars and smell them. I opened them up because I saw no growth smelled it then tossed.
I live in Scandinavia I don't know if that would be considered high elevation?


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InvisibleCataSTRAWfic_Grows
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Registered: 09/12/17
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Re: Jars not showing growth... [Re: Booooooooom]
    #24862389 - 12/22/17 06:07 PM (3 years, 4 months ago)

have you used this lc before successfully/ is it a new culture?


--------------------
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OfflineBooooooooom
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Re: Jars not showing growth... [Re: CataSTRAWfic_Grows]
    #24863114 - 12/23/17 03:41 AM (3 years, 4 months ago)

Quote:

CataSTRAWfic_Grows said:
have you used this lc before successfully/ is it a new culture?



No this is a new culture never used before, its a Texas PE6 strain.


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OfflineBooooooooom
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Re: Jars not showing growth... [Re: Booooooooom]
    #24997976 - 02/16/18 05:34 AM (3 years, 2 months ago)

Its been 4 weeks, very minimal to no growth. All My pf tek jars showed growth after 24hours and took of. The grain jars look dead. Could it be a GE problem. I have polyfill lids that are tight fitting?


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InvisibleMatejaS
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Re: Jars not showing growth... [Re: Booooooooom]
    #24998052 - 02/16/18 06:44 AM (3 years, 2 months ago)

The poly is not too tight, GE is not the issue.
Have you fruited the BRF cakes that were from the same LC? Did you make the LC from spore solution?


--------------------
p9hu7 said:
So, why bother saying "why bother"? it is what it is. The purpose is to experiment with procedures that venture outside the norm, is it necessary for growing mushrooms? Big nope. Is it fucking cool to think and experiment? Resounding yes.


Edited by Mateja (02/16/18 06:46 AM)


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OfflineBooooooooom
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Re: Jars not showing growth... [Re: Mateja]
    #24998317 - 02/16/18 11:01 AM (3 years, 2 months ago)

Quote:

Mateah said:
The poly is not too tight, GE is not the issue.
Have you fruited the BRF cakes that were from the same LC? Did you make the LC from spore solution?



Havent fruited the BRF yet but growth is steady and no contams.
Spores were done to lc then lc was tested on agar. Clean looking growth.(Ecuador)
I have another lc that was cleaned on agar Texas PE6 same results anyhow.
Something is Up. It may be moisture content issues.


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Offlinesilverado700
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Re: Jars not showing growth... [Re: Booooooooom]
    #24998393 - 02/16/18 11:45 AM (3 years, 2 months ago)

Is the rye organic? Could have a fungicide in it


--------------------
PS.. Thats what the alphabet would look like without the letter Q and R    :shocked:


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OfflineBooooooooom
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Re: Jars not showing growth... [Re: silverado700]
    #24998672 - 02/16/18 02:07 PM (3 years, 2 months ago)

Quote:

silverado700 said:
Is the rye organic? Could have a fungicide in it



It is organic! I dont think it has fungicide, could it survive a pressurecooking run?


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InvisibleMatejaS
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Re: Jars not showing growth... [Re: Booooooooom]
    #24998875 - 02/16/18 04:07 PM (3 years, 2 months ago)

Quote:

Booooooooom said:
Quote:

Mateah said:
The poly is not too tight, GE is not the issue.
Have you fruited the BRF cakes that were from the same LC? Did you make the LC from spore solution?



Havent fruited the BRF yet but growth is steady and no contams.
Spores were done to lc then lc was tested on agar. Clean looking growth.(Ecuador)
I have another lc that was cleaned on agar Texas PE6 same results anyhow.
Something is Up. It may be moisture content issues.



Spores first to LC and then to agar hehehe, God bless you :cool:

Definitely sounds like your grain prep if you have good growth on BRF from the same LC.
I have not prepped anything else besides WBS but I somehow feel that 10-15min simmer is a bit short for hydrating grain to the max.

I did experiments with hydration methods with WBS it´s in my main threads you can check it out if you want.

Also if the surface of the grain dries out then myc will have a really hard time colonizing it.
But in your cakse you don´t even have a leap off on grain so that´s really weird.

The best tip I can give you is do more field capacity tests on your grain during prepping and take notes. GL


--------------------
p9hu7 said:
So, why bother saying "why bother"? it is what it is. The purpose is to experiment with procedures that venture outside the norm, is it necessary for growing mushrooms? Big nope. Is it fucking cool to think and experiment? Resounding yes.


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OfflineBooooooooom
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Re: Jars not showing growth... [Re: Mateja]
    #25000916 - 02/17/18 12:00 PM (3 years, 2 months ago)

Quote:

Mateah said:
Quote:

Booooooooom said:
Quote:

Mateah said:
The poly is not too tight, GE is not the issue.
Have you fruited the BRF cakes that were from the same LC? Did you make the LC from spore solution?



Havent fruited the BRF yet but growth is steady and no contams.
Spores were done to lc then lc was tested on agar. Clean looking growth.(Ecuador)
I have another lc that was cleaned on agar Texas PE6 same results anyhow.
Something is Up. It may be moisture content issues.



Spores first to LC and then to agar hehehe, God bless you :cool:

Definitely sounds like your grain prep if you have good growth on BRF from the same LC.
I have not prepped anything else besides WBS but I somehow feel that 10-15min simmer is a bit short for hydrating grain to the max.

I did experiments with hydration methods with WBS it´s in my main threads you can check it out if you want.

Also if the surface of the grain dries out then myc will have a really hard time colonizing it.
But in your cakse you don´t even have a leap off on grain so that´s really weird.

The best tip I can give you is do more field capacity tests on your grain during prepping and take notes. GL



How do i know when the grain is at
t field capacity?
How do you prepare tour grain?


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InvisibleMatejaS
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Re: Jars not showing growth... [Re: Booooooooom]
    #25001886 - 02/17/18 08:07 PM (3 years, 2 months ago)

I do a 60 min hot soak, like in this

And then you can see the results with colonizing jars here. GL


--------------------
p9hu7 said:
So, why bother saying "why bother"? it is what it is. The purpose is to experiment with procedures that venture outside the norm, is it necessary for growing mushrooms? Big nope. Is it fucking cool to think and experiment? Resounding yes.


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OfflineBooooooooom
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Re: Jars not showing growth... [Re: Mateja]
    #25003022 - 02/18/18 10:00 AM (3 years, 2 months ago)

Quote:

Mateah said:
I do a 60 min hot soak, like in this

And then you can see the results with colonizing jars here. GL




Wow man very nice experiment indeed, i Will definitely try this thanks alot. Do you think rye berries are the same with the hot soak?


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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