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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 3 months
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WL-Tek [Test log] 2
#24842299 - 12/12/17 04:02 PM (6 years, 2 months ago) |
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Firstly, it turned out I had a ongoing bacillus issue, I will be replicating the setup with clean mycelium very soon.
Previous tests with Golden oyster produced 620g from 600g, in less than optimal fruiting settings. It continued to produce another 295g outdoors, with cellulose rich compost.


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Recipe (See here for the full guide):
Desired Water Content + Cook Water (Boiling Hot) | 125g > Pellets | 6g > MG Soluble Nutrients | 1g > YN Soluble Nutrients.
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It took 2 weeks paper > paper, however secondary bacterial continuation from the spawn.

It fruited prolifically, prior to a full fruiting setup.

Edited by Ferather (06/07/22 10:16 AM)
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tiedma
Fat Kid


Registered: 08/16/17
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Re: WL-Tek [Test log] [Re: Ferather]
#24842321 - 12/12/17 04:18 PM (6 years, 2 months ago) |
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
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Re: WL-Tek [Test log] [Re: tiedma]
#24842340 - 12/12/17 04:26 PM (6 years, 2 months ago) |
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Feel free to add your own additive's, they ideally should be carbon free, no starch or sugar. They can be organic, or inorganic, some research and experience is required.
Test small samples where possible, as it saves money and effort.
No added sugar is needed with phenol containing wood. Paper is essentially all cellulose, no phenol's.
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Ferather
Mycological



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Posts: 6,325
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Re: WL-Tek [Test log] [Re: Ferather]
#24846052 - 12/14/17 03:46 PM (6 years, 2 months ago) |
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I have updated my cellulose agar alternative guide, and will be continuing this thread soon.
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Ferather
Mycological



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Re: WL-Tek [Test log] [Re: Ferather]
#24850224 - 12/16/17 04:33 PM (6 years, 2 months ago) |
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How to cook WL-Tek using a microwave pressure cooker (recipe in the first post, 125g dry):
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Note: I am adding extra water to my hydration measurement, allowing me to increase the cooking time.
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Using a jug, measure your additives, using boiling hot water, add the water needed. Mix together using a clean fork, the solubles will dissolve automatically.
Measure your pellets into a bowl 5-6x bigger than the pellets. Add the nutrient water to the pellets then mix-n-mash.
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Transfer the mush to the microwave pressure cooker, fairly loose, push down lightly when full. Cook for 12 minutes, wait 40 minutes, mix it up, then cook again for 8 minutes.
Make sure the top vents are closed (rice cooking guidelines).
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You can also do the capacity you want + 20g, then pressure cook (autoclave).
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Ferather
Mycological



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Posts: 6,325
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Re: WL-Tek [Test log] [Re: Ferather]
#24851205 - 12/17/17 07:45 AM (6 years, 2 months ago) |
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I transferred a wood peg from T-Gel to WL-Tek, recipe in the first post, the cook procedure is above. 100g was assembled into a 250ml container, microwaved for 48 seconds, then lid on.
The lids I am using are modified with a hole and filter, so no vacuum.
There is regeneration on the peg after just 4 hours.
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Ferather
Mycological



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Posts: 6,325
Loc: United Kingdom
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Re: WL-Tek [Test log] [Re: Ferather]
#24851934 - 12/17/17 02:43 PM (6 years, 2 months ago) |
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Turns out it was probably internal mycelium pushing to air (O2), it's being normal now.
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Ferather
Mycological



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Re: WL-Tek [Test log] [Re: Ferather]
#24853198 - 12/18/17 08:08 AM (6 years, 2 months ago) |
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24 hours, it's still growing in the areas where it leaped after just 4 hours. However, it's being normal, best growth observed in high O2.
The peg absorbs solubles from the media.
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Ferather
Mycological



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Re: WL-Tek [Test log] [Re: Ferather]
#24854368 - 12/18/17 06:01 PM (6 years, 2 months ago) |
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34 hours, it is now colonizing the substrate.
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Ferather
Mycological



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Re: WL-Tek [Test log] [Re: Ferather]
#24855276 - 12/19/17 06:26 AM (6 years, 2 months ago) |
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46 hours, it seems faster and more populated on the enriched cellulose. Grain speed, but cellulose (slow to decay) based population.
Hopefully it will be well fed in within 4 days.
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Ferather
Mycological



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Re: WL-Tek [Test log] [Re: Ferather]
#24856361 - 12/19/17 05:54 PM (6 years, 2 months ago) |
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Equal in speed on the peg, and the substrate.
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Ferather
Mycological



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Re: WL-Tek [Test log] [Re: Ferather]
#24860645 - 12/21/17 05:10 PM (6 years, 2 months ago) |
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Conclusion, based on experience, and testing:
It is faster, and potentially safer, to use clean seed spawn (high in cellulose), and add no sugar to the main substrate. The spawn will act as the starter carbon, removing the need to add sucrose as carbon to the entire media.
This reduces contamination chances significantly, and allows for fully exposed assembly. Paper pellets, are composed of complex, slow to react (slow release), cellulose.
The spawn will produce well fed mycelium, from the start.
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I have moved sucrose back to aseptic no spawn.
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Ferather
Mycological



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Re: WL-Tek [Test log] [Re: Ferather]
#24860659 - 12/21/17 05:18 PM (6 years, 2 months ago) |
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Additional data:
Wood naturally contains plant phenol's, which lignicolous mycelium decay with laccase enzymes as starter carbon. Thus wood pellets themselves should never have starch or sugar added, as it's not needed, and has limits.
The best starch enzyme essentially prefers a neutral pH, mycelium make substrates a lower pH. Wood naturally has an acidic pH, therefor starch is not very effective, and it's 'in vitro'.
Sugar's however do not appear to have a pH restriction, and work at all times. Adding sugar or starch, will open the wood up to 'all' contamination.
My T-Gel agar has already proven the decay of phenol's.
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Ferather
Mycological



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Posts: 6,325
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Re: WL-Tek [Test log] [Re: Ferather]
#24873536 - 12/28/17 12:55 PM (6 years, 2 months ago) |
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I did some testing on paper pellets, it turns out they contain multiple types of dormant bacteria. This is due to contact handling and contact with food such as broken eggs (carton).
Once cleaned, fully, paper pellets are much easier to use. I got 4 months with WL-Tek (plain) @ 20 minutes.
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Ferather
Mycological



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Posts: 6,325
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Re: WL-Tek [Test log] [Re: Ferather]
#24873537 - 12/28/17 12:55 PM (6 years, 2 months ago) |
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Microwave guide, 800w (watts):
I am using a cheap microwave pressure cooker, and using vents closed mode (rice cook).
Recommended: 20 minutes or more, fully hydrated with boiling hot water. Minimum: 12 minutes, fully hydrated with boiling hot water.
Tip: The odd smell should disappear (ink).
I needed 120g of extra water.
Edited by Ferather (12/28/17 01:07 PM)
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Ferather
Mycological



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Posts: 6,325
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Re: WL-Tek [Test log] [Re: Ferather]
#24900794 - 01/09/18 01:45 PM (6 years, 1 month ago) |
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Wood peg onto WL-Tek plain (no tea, no CaCO3), no spawn, media test.
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This is a test, for best results use starch rich "clean" spawn!
Test: Open air assembly, using enriched cellulose.
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Media, carbon:
The toothpick peg contains cellulose and trace phenol's, no sugar or starch. WL-Tek plain is basically all cellulose, no phenol's, sugar or starch.
Tip: Cellulose is almost inert, and decay's very slowly.
Media, nitrogen:
Supplied by the MG and YN additives, soluble, easy to absorb.
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Live transfer:
Live transfers usually have enough internal resources to digest most medias. Generally they do not need soluble carbon sources, no germination.
Soluble's: Starch, sugar, phenol's, protein's and other.
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Growth is weak to begin with (expected), the rate of decay improves over time. As more cells and enzymes are produced, more carbon is received.
Currently I have carbon weak, nitrogen rich growth.
Edited by Ferather (01/09/18 01:52 PM)
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
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Re: WL-Tek [Test log] [Re: Ferather]
#24903333 - 01/10/18 03:00 PM (6 years, 1 month ago) |
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WL-Tek, no spawn (ideal for culture storage):
In terms of speed, it's equal to grain, due to the increased rate provided by the added "soluble" nutrients. At the moment it's still accumulating carbon from the "slow release" cellulose via "enzymes".
Population and density increases daily as does the outward push (improved). As mentioned above, it will eventually become "well fed" visibly.
The Tarragon is penetrating the "open structure".
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: WL-Tek [Test log] [Re: Ferather]
#24951673 - 01/29/18 07:15 AM (6 years, 1 month ago) |
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T-Gel wedge to WL-Tek:
Here I am using the latest recipe, microwave pressure cooked into intended capacity.
The end weight is 90g, 25g pellets + 63g water = 72% water content. This is the maximum, you may prefer 50-66% water content.
Response after 10 hours, sorry for the quality.
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Ferather
Mycological



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Re: WL-Tek [Test log] [Re: Ferather]
#24954887 - 01/30/18 03:06 PM (6 years, 1 month ago) |
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Normal speed, however low population rate, due to slow release cellulose. The slow release produces low carbon settings for a while. I'm using the optional tea leaves recipe, which is being added to add phenol's, other, to the acid free paper.
Reminder: Spawn should be used for a full population rate, removing the low carbon setting. Benefit: No starch, sugar or other is being added directly to the media.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 3 months
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Re: WL-Tek [Test log] [Re: Ferather]
#24963684 - 02/03/18 08:55 AM (6 years, 27 days ago) |
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Working as intended, currently I have higher O2 surface growth, it's spread and begun growing downwards. The downward growth is thicker, and more linear, the surface growth is cottony and thin.
There is light condensate specifically at locations populated by mycelium. At 72% water content, the paper is able to absorb more water.
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The media is microwave pressure cooked into capacity, starting with a higher amount due to losses.
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Images soon, it's still becoming well fed, I expect 3 weeks to colonize. Then another week to fully populate, speed is high-normal.
With spawn I would be fruiting after 2-2.5 weeks.
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Reminder: Cellulose is relatively inert, non-reactive, and is slow to decay.
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