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InvisibleMateja
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Re: Post Your Cultivation Picture of The Day [Re: LizardWizard]
    #24859742 - 12/21/17 10:25 AM (6 years, 4 months ago)

When I have the opportunity I will make BRF pucks inside qt jars and then print the invitro fruits, wouldnยดt that give me the cleanest spore prints possible? I also already did some experiments with placing caps on top of a metal wire inside half pint jars so that the spores land directly into the sterile water underneith but the surface tension of the water does not mix the spores and they all crystallize on the surface like ice and break up only into large pieces with thousands of spores stuck together. I will tweak the recipe until I come up with a solution that the spores can get mixed into as they fall into the sterile solution.


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InvisibleMycolorado
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Re: Post Your Cultivation Picture of The Day [Re: Mateja]
    #24859749 - 12/21/17 10:28 AM (6 years, 4 months ago)

Add a minuscule amount of soap to break the surface tension...maybe touch a sterile needle to some dish soap and touch the surface of the water.


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Invisiblestareatclouds
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Re: Post Your Cultivation Picture of The Day [Re: Mateja]
    #24859753 - 12/21/17 10:29 AM (6 years, 4 months ago)

An in-vitro fruit would be cleaner than not, sure. But at some point stressing to get as clean a print as possible is overkill to a simple process. It's going to agar anyway so you can transfer away from bacteria. When I've done prints, I just placed the cap on sterilized foil in a PP5 pint inside my SAB with the lid slightly covering it. One poster said it was one of the cleanest prints he had and got clean germination immediately.

But I'm all for experimentation. That seems like a fun thing to try, Mateah.

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InvisibleTheMadHatter420
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Re: Post Your Cultivation Picture of The Day [Re: stareatclouds]
    #24859758 - 12/21/17 10:32 AM (6 years, 4 months ago)

If you want a cleaner print this is how you do it. Print your cap for 6 or so hours. Then move the cap to a new clean section of foil. Discard spores from the 1st one. Theoretically bacteria and their spores should have dropped off, or at least a large majority of them.

EDIT

Just so you know, working with agar will be much easier and less stress than fighting to get a "clean" print so you can attempt to shoot spores onto grains.


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Edited by TheMadHatter420 (12/21/17 10:34 AM)

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InvisibleMateja
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Re: Post Your Cultivation Picture of The Day [Re: stareatclouds]
    #24859766 - 12/21/17 10:37 AM (6 years, 4 months ago)

Yeah I was mostly looking to create a really clean and at the same time really spore dense syringe that can be diluted further with sterile water and thus create virtually clean spore syringes which should be able to be used on grain without many hickups. Of course I work with agar so this is not necessary, but it would for sure give me some head rest to know that I have really dense and really clean spore syringes stored somewhere that can even be further diluted just i case I plan on giving them to a really dirty friend who is doing the PF Tek for example :smile:

Colorado, thanks for the tip I though of this as well and am including it in the next experiments.


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InvisibleTheMadHatter420
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Re: Post Your Cultivation Picture of The Day [Re: Mateja]
    #24859796 - 12/21/17 10:54 AM (6 years, 4 months ago)

LOL, ANY spore syringe can be diluted farther. There is more spores in one syringe than needed by far. In fact I diluted a vendor syringe down and it worked out rather well. Like your thinking diluting the spores also dilutes the contamination.

Your probably better off storing as a print than a syringe, and the print is the step before syringe so it is no extra work.


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Offlinetryptkaloids
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Re: Post Your Cultivation Picture of The Day [Re: LizardWizard]
    #24859810 - 12/21/17 11:01 AM (6 years, 4 months ago)

Quote:

LizardWizard said:
Quote:

tryptkaloids said:
:whathesaid: most of the time when my grain jars are bacterial it's woven into the culture
Quote:

van hatton said:
Liz I no our quarts are slightly smaller if I remember right. I assume the lids are different. Order some lids from the us first :rockon:



I didn't know that. Its not the threads that matter, it's the diameter of the lid, as long as the blade fits your good, you do have the ring style lids right?




no ring style lids. Just one piece lids. So the oster blade is clamped in one of those ring lids instead? And the standard oster blades are all sterilizable then? Because if so, it doesn't seem like it would be too hard to just make a ring lid by cutting out the middle portion... If the right lid was chosen, just a hole for the attaching spinning piece would be good..



Exactly, you just need a solid connection with the base


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OfflineFishLevelMidnight
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Re: Post Your Cultivation Picture of The Day [Re: TheMadHatter420]
    #24859828 - 12/21/17 11:05 AM (6 years, 4 months ago)

In biological specimen prep, we often use triton X-100 or tween20 as detergents. Using a final concentration of 0.1% would probably help to break up spores.

At some point Iโ€™d like to use 0.1% triton to see if I can easily separate spore clumps and dilute it down enough to get single spores; maybe use a vortex to break it up also.

Sounds like it could be something fun for Bod to do in the near future :wink:


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InvisibleComebackKid Happy Birthday!
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Re: Post Your Cultivation Picture of The Day [Re: TheMadHatter420]
    #24859845 - 12/21/17 11:09 AM (6 years, 4 months ago)

I once made a syringe out of an agar pin plate that sporelated.
Never tested the syringe tho it's still kicling around with my myco stuff


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Invisiblevan hattonFacebookDiscord
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Re: Post Your Cultivation Picture of The Day [Re: tryptkaloids]
    #24859873 - 12/21/17 11:19 AM (6 years, 4 months ago)

Quote:

tryptkaloids said:
Quote:

LizardWizard said:
Quote:

tryptkaloids said:
:whathesaid: most of the time when my grain jars are bacterial it's woven into the culture
Quote:

van hatton said:
Liz I no our quarts are slightly smaller if I remember right. I assume the lids are different. Order some lids from the us first :rockon:



I didn't know that. Its not the threads that matter, it's the diameter of the lid, as long as the blade fits your good, you do have the ring style lids right?




no ring style lids. Just one piece lids. So the oster blade is clamped in one of those ring lids instead? And the standard oster blades are all sterilizable then? Because if so, it doesn't seem like it would be too hard to just make a ring lid by cutting out the middle portion... If the right lid was chosen, just a hole for the attaching spinning piece would be good..



Exactly, you just need a solid connection with the base





The one and only time I pced a Oster blender part it warped to the jar and ruined the attachment. It no longer holds water. My gf is still pissy lol


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Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

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OfflineSpotter
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Re: Post Your Cultivation Picture of The Day [Re: van hatton] * 1
    #24859892 - 12/21/17 11:28 AM (6 years, 4 months ago)


Malbar


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InvisibleMateja
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Re: Post Your Cultivation Picture of The Day [Re: TheMadHatter420]
    #24859928 - 12/21/17 11:42 AM (6 years, 4 months ago)

Quote:

TheMadHatter420 said:
LOL, ANY spore syringe can be diluted farther. There is more spores in one syringe than needed by far. In fact I diluted a vendor syringe down and it worked out rather well. Like your thinking diluting the spores also dilutes the contamination.

Your probably better off storing as a print than a syringe, and the print is the step before syringe so it is no extra work.



Yes most spore syringes can be diluted, and when you run out of spore solution you can suck up sterile water into the same syringe and squeeze out a few more mm of spore solution :smile: but as I mentioned Iยดm experimenting with a method where the clean invitro hats would be placed directly over sterile solution which is then simply sucked back into a syringe for storage, no need to first make prints and then make syringes get it :wink: also what I actually mean with "dense spore syringes that can be diluted" is a 'clean' syringe that is so dense that the syringe that is not be used directly for inoculation but only for making new sterile syringes that can also be diluted if one so wishes, and all exceedingly more and more clean, the goal is of course to produce spore syringes that have the same success rate on grain as a2g. I appreciate all tips and advice at all time :smile:


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InvisibleTheMadHatter420
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Re: Post Your Cultivation Picture of The Day [Re: Mateja]
    #24859938 - 12/21/17 11:47 AM (6 years, 4 months ago)

Putting that cap over the solution give another vector for contamination.

You could keep it jarred up instead of in a syringe. I actually have a pint with about 200ml of spore solution in it. I feel like the jar is less likely to let things in vs the syringe.


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InvisibleJHOVA
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Re: Post Your Cultivation Picture of The Day [Re: TheMadHatter420]
    #24860012 - 12/21/17 12:17 PM (6 years, 4 months ago)

Well thats interesting. How would you go about fixing a mesh in a jar?


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InvisibleTheMadHatter420
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Re: Post Your Cultivation Picture of The Day [Re: JHOVA]
    #24860038 - 12/21/17 12:29 PM (6 years, 4 months ago)

Why would you need to fix a mesh in a jar?


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InvisibleLizardWizard
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Re: Post Your Cultivation Picture of The Day [Re: saralove]
    #24860074 - 12/21/17 12:43 PM (6 years, 4 months ago)

Quote:

van hatton said:
Quote:

tryptkaloids said:
Quote:

LizardWizard said:

no ring style lids. Just one piece lids. So the oster blade is clamped in one of those ring lids instead? And the standard oster blades are all sterilizable then? Because if so, it doesn't seem like it would be too hard to just make a ring lid by cutting out the middle portion... If the right lid was chosen, just a hole for the attaching spinning piece would be good..



Exactly, you just need a solid connection with the base





The one and only time I pced a Oster blender part it warped to the jar and ruined the attachment. It no longer holds water. My gf is still pissy lol




Well which part warped?

The plastic ring part I assume? Because the only part that's not supposed to warp in this setup is the blade piece, as long as that can take the heat, gasket n all, there wouldn't be a problem..

I just ordered myself a vintage Oster base and 4 new ice crusher blades. Let's see how well this thing holds up using European jars...


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InvisibleLizardWizard
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Re: Post Your Cultivation Picture of The Day [Re: TheMadHatter420]
    #24860077 - 12/21/17 12:46 PM (6 years, 4 months ago)

to put a cap on top of the mesh to let it drop spores in the jar?

You MacGyver it.


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InvisibleJosex
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Re: Post Your Cultivation Picture of The Day [Re: Mateja]
    #24860086 - 12/21/17 12:49 PM (6 years, 4 months ago)

Quote:

Mateah said:
Iยดm experimenting with a method where the clean invitro hats would be placed directly over sterile solution which is then simply sucked back into a syringe for storage, no need to first make prints and then make syringes get it :wink: also what I actually mean with "dense spore syringes that can be diluted" is a 'clean' syringe that is so dense that the syringe that is not be used directly for inoculation but only for making new sterile syringes that can also be diluted if one so wishes, and all exceedingly more and more clean, the goal is of course to produce spore syringes that have the same success rate on grain as a2g. I appreciate all tips and advice at all time :smile:




https://www.shroomery.org/forums/showflat.php/Number/16044347#16044347

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InvisibleTheMadHatter420
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Re: Post Your Cultivation Picture of The Day [Re: LizardWizard]
    #24860115 - 12/21/17 12:57 PM (6 years, 4 months ago)

I would just heat a piece of wire glowing red, and stick it through the cap from side to side, or even hang it from the lid. LOL fishhook that bitch from the lid.


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InvisibleJHOVA
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Re: Post Your Cultivation Picture of The Day [Re: TheMadHatter420]
    #24860158 - 12/21/17 01:13 PM (6 years, 4 months ago)

Thats cool.


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