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OfflineMushiesAreVegan
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I wanna make Liquid Inocculant, wich plate to choose from?
    #24853305 - 12/18/17 09:07 AM (6 years, 3 months ago)

Hey everyone

So i recently started working with agar, with p.cubensis and p.cyanescens. Some of my dishes seem to be contaminated, and others i may wanna use to make liquid innoculant with the cubes. what plate would be best suited for that?

My first batch i made december first, some of the cultures seem to be contaminated

pic 1

cyanescens with yellow and red contam (almost invisible, next to the yeast and between the two spots of mycelium)

pic 2

cubensis

pic3


and another cubensis that seems to be fine

my second batch of transfers i made on dec 12, they seem to be clean. the agar wedge slithered around on the plate a bit, thats why there is growth at different places (i think so).

pic 4

cubensis with rhizomorphs all over the upper plate (i think)

pic 5

Cubencis that seems to be fine

pic 6

cyanenscens wedge that also slithered around on the plate


thanks a lot for help :smile:

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InvisibleJHOVA
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Re: I wanna make Liquid Inocculant, wich plate to choose from? [Re: MushiesAreVegan]
    #24853316 - 12/18/17 09:16 AM (6 years, 3 months ago)

They need more uniformity from further transfers in my opinion.


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OfflineWeavieWonder
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Re: I wanna make Liquid Inocculant, wich plate to choose from? [Re: JHOVA]
    #24853328 - 12/18/17 09:19 AM (6 years, 3 months ago)

:whathesaid:

Keep transferring.

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InvisiblebodhisattaMDiscordReddit
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Re: I wanna make Liquid Inocculant, wich plate to choose from? [Re: MushiesAreVegan]
    #24853337 - 12/18/17 09:21 AM (6 years, 3 months ago)

Skip liquids entirely until your agar work is up to par.

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OfflineMushiesAreVegan
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Re: I wanna make Liquid Inocculant, wich plate to choose from? [Re: bodhisatta]
    #24853352 - 12/18/17 09:27 AM (6 years, 3 months ago)

ok, so im going to do a transfer today then, and probably just do the inoculation with a multispore syringe i still have

thanks for the tips :smile:

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InvisiblebodhisattaMDiscordReddit
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Re: I wanna make Liquid Inocculant, wich plate to choose from? [Re: MushiesAreVegan]
    #24853356 - 12/18/17 09:29 AM (6 years, 3 months ago)

Inoculate with an agar wedge. Just don't go agar to liquids until your Agar work is excellent

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OfflineMushiesAreVegan
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Re: I wanna make Liquid Inocculant, wich plate to choose from? [Re: MushiesAreVegan]
    #24853393 - 12/18/17 09:52 AM (6 years, 3 months ago)

will do, just prepped my sab for another transfer. mycology is fun :grin:

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OfflineMorel Guy
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Re: I wanna make Liquid Inocculant, wich plate to choose from? [Re: MushiesAreVegan]
    #24853409 - 12/18/17 10:02 AM (6 years, 3 months ago)

I see 2 that I would transfer and clean.


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"in sterquiliniis invenitur in stercore invenitur"

In filth it will be found in dung it will be found

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InvisibleJHOVA
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Re: I wanna make Liquid Inocculant, wich plate to choose from? [Re: Morel Guy]
    #24853434 - 12/18/17 10:25 AM (6 years, 3 months ago)

Post pics in a couple days of your agar. Its looking better already after 1 transfer.


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OfflineMushiesAreVegan
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Re: I wanna make Liquid Inocculant, wich plate to choose from? [Re: MushiesAreVegan]
    #24853445 - 12/18/17 10:29 AM (6 years, 3 months ago)

will do. i only have 5 plates left, i gotta buy more dishes and more agar. Doing 7 species of mushrooms on agar at once burns through them really, really fast

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OfflineMorel Guy
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Re: I wanna make Liquid Inocculant, wich plate to choose from? [Re: MushiesAreVegan]
    #24853874 - 12/18/17 02:09 PM (6 years, 3 months ago)

Don't over commit yourself.  Why so many species?

There is so much variation among one variety and species.  Lot's to thoroughly examine.

KISS

Keep
It
Simple
Stoner


--------------------
"in sterquiliniis invenitur in stercore invenitur"

In filth it will be found in dung it will be found

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OfflineMushiesAreVegan
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Re: I wanna make Liquid Inocculant, wich plate to choose from? [Re: Morel Guy]
    #24853931 - 12/18/17 02:43 PM (6 years, 3 months ago)

Quote:

Morel Guy said:
Don't over commit yourself.  Why so many species?




because i have a big project, and i got in a bit deep^^

its 2 psilocybe and 5 gourmet mushrooms, oysters, shitake, yellow oyster (dunno how its called in english), enokii and stropharia rugosannulata.

mostly because i want to have spawn for multiple gourmet species ready in spring that me and my friens who help me can use in our garden projects

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