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InvisibleMad Season
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Re: Does mycelium only colonize the top of an agar dish [Re: Spindlymass]
    #24882880 - 01/01/18 10:36 AM (7 years, 16 days ago)

There's definitely bacteria in that pan plate, but yeah those blue balls are pseudo-sclerotia.


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InvisibleAlexSmitt
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Registered: 09/10/16
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Re: Does mycelium only colonize the top of an agar dish [Re: Mad Season]
    #24891375 - 01/05/18 08:00 AM (7 years, 12 days ago)

Unfortunately I'm a little late on taking the pics I wanted to share here. My 2 cents, I'm gonna say it only colonizes the surface. I placed a transfer wedge on its side a few days ago and it kept growing in the same direction, the more prominent strands grew off the agar and into the air BEFORE it started wrapping around the rest of the agar and growing along the new plate. Unfortunately I forgot to take the pic yesterday morning after the transfer but here it is today.

Yesterday there was 3-4 nice strands standing proud off the agar that are now slumped over. Try it everyone, put your next transfer on its side and observe.


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InvisibleViolet
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Registered: 12/06/11
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Re: Does mycelium only colonize the top of an agar dish [Re: AlexSmitt]
    #24891649 - 01/05/18 11:09 AM (7 years, 12 days ago)

That looks more like mycelium being oriented in one direction from leading edge growth. I even get a similar effect flipping fast strains up side down on new agar.  The myc only recently colonized the agar and continues its directional growth a bit more quickly than it turns around to get the agar behind it too.


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InvisibleAlexSmitt
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Re: Does mycelium only colonize the top of an agar dish [Re: Violet]
    #24892355 - 01/05/18 05:02 PM (7 years, 12 days ago)

Which is why I think it stays on the outside. My first time using an inoculation loop to get myc from agar to lc it just scraped so perfectly off the top leaving a bare area underneath. Now I take a little agar too so it doesn't float and get stuck on the side of the glass or something.


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InvisibleViolet
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Re: Does mycelium only colonize the top of an agar dish [Re: AlexSmitt]
    #24892382 - 01/05/18 05:13 PM (7 years, 12 days ago)

You’re only seeing the aerial mycelium. Myc definitely colonizes the whole agar. That’s why Master slants work.


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InvisibleAlexSmitt
Smitty Mushroomjagermanjenson

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Re: Does mycelium only colonize the top of an agar dish [Re: Violet]
    #24892991 - 01/05/18 09:56 PM (7 years, 12 days ago)

I've never made a slant, not yet at least. So if I have it long enough to use the whole surface I can still scrape some from the whole tube? I'm not too advanced yet, I spent like 2 years doing pf cakes. Is there a way to view it? I saw someone earlier said it's clear like snow and a polar bears fur, it's about the way the light hits it. Just for my own self satisfaction I'm going to pour a thick plate, let it colonize, then cut away the top layer and see what happens. Just to see. I'll take the word of someone with 10x more posts than myself.


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InvisibleUllr
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Registered: 10/27/17
Posts: 93
Re: Does mycelium only colonize the top of an agar dish [Re: AlexSmitt]
    #24893068 - 01/05/18 10:29 PM (7 years, 12 days ago)

- I've had bacteria grow under my agar, and there was a visible weakening of the mycelium growing at the surface, matching the bacterial area.
- Tea or dyed agar discolors down to the bottom.
- Transfer wedges seem to "sprout" all around with thin mycelium threads if you look closely, and not just surface colonization.

So yeah, I'd say mycelium actually reaches into the agar, even if not visible to the naked eye.

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