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OfflineShroomySammy
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4 weeks to the day
    #24846522 - 12/14/17 08:02 PM (7 years, 1 month ago)

OK its been 4 weeks to the day since inoculation and thy have started to pin in the jars without 100% colonization what should I do and how can I stop this from happening in the future?


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OfflineCakewonder
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Re: 4 weeks to the day [Re: ShroomySammy]
    #24846533 - 12/14/17 08:10 PM (7 years, 1 month ago)

Contaminants can cause in vitro pinning I believe. In vitro pinning can also be caused by exposing the jars to far too much light during colonization. Give us pictures.


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InvisibleLemurLemur
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Re: 4 weeks to the day [Re: ShroomySammy]
    #24846538 - 12/14/17 08:11 PM (7 years, 1 month ago)

How'd you prep yo sub? Sounds like bacterial sub.

Light is not a pinning trigger


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OfflineShroomySammy
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Re: 4 weeks to the day [Re: LemurLemur]
    #24846550 - 12/14/17 08:18 PM (7 years, 1 month ago)




Here are some pictures 4 different jars


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OfflineCakewonder
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Re: 4 weeks to the day [Re: LemurLemur]
    #24846554 - 12/14/17 08:19 PM (7 years, 1 month ago)

Quote:

LemurLemur said:
How'd you prep yo sub? Sounds like bacterial sub.

Light is not a pinning trigger




Seems that light is a pinning trigger after all.


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Edited by Cakewonder (12/14/17 09:14 PM)

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OfflineShroomySammy
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Re: 4 weeks to the day [Re: ShroomySammy]
    #24846557 - 12/14/17 08:20 PM (7 years, 1 month ago)

And I steamed them for 3 hours i know it's not ideal but I've heard that it work


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InvisibleTheMilkMan84
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Re: 4 weeks to the day [Re: LemurLemur] * 1
    #24846558 - 12/14/17 08:20 PM (7 years, 1 month ago)

If they’re almost all the way colonized you can always birth them and rinse off any uncolonized substrate, and then proceed with the dunk and roll

Edit: I personally would let those ones go a little longer though, but when you’re ready you can rinse off any uncolonized substrate


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Edited by TheMilkMan84 (12/14/17 08:23 PM)

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InvisibleLemurLemur
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Re: 4 weeks to the day [Re: ShroomySammy]
    #24846571 - 12/14/17 08:32 PM (7 years, 1 month ago)

Go ahead and fruit them, there not gonna want to colonize during fruiting and theres a reason why they, bacteria. Next time boil for 6 hours with weight on the lid although it could have come from the ms solution.

Let them fruit and finish then dunk and roll, though you can patch verm on with your hand and let it sit in water, the myc network will soak it up.


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(when my data is fast play Lemur in chess at chess.com)[ [gradient:#D40B29,#18C418]Any1 expecting a trade from me i havent forgot about you pinky promise, i fr promise shits just shit rt now[/gradient]

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OfflineShroomySammy
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Re: 4 weeks to the day [Re: TheMilkMan84]
    #24846574 - 12/14/17 08:33 PM (7 years, 1 month ago)

How long would you let them go for?


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OfflineCakewonder
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Re: 4 weeks to the day [Re: LemurLemur]
    #24846578 - 12/14/17 08:34 PM (7 years, 1 month ago)

LEmur!

Thanks for correcting me:)


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InvisibleLemurLemur
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Re: 4 weeks to the day [Re: ShroomySammy]
    #24846580 - 12/14/17 08:35 PM (7 years, 1 month ago)

Fruit them...now!


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(when my data is fast play Lemur in chess at chess.com)[ [gradient:#D40B29,#18C418]Any1 expecting a trade from me i havent forgot about you pinky promise, i fr promise shits just shit rt now[/gradient]

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OfflineShroomySammy
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Re: 4 weeks to the day [Re: LemurLemur]
    #24846585 - 12/14/17 08:38 PM (7 years, 1 month ago)

OK I will but will they be safe to consume?


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InvisibleLemurLemur
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Re: 4 weeks to the day [Re: ShroomySammy] * 1
    #24846587 - 12/14/17 08:40 PM (7 years, 1 month ago)

:megacrankey:......


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(when my data is fast play Lemur in chess at chess.com)[ [gradient:#D40B29,#18C418]Any1 expecting a trade from me i havent forgot about you pinky promise, i fr promise shits just shit rt now[/gradient]

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OfflineShroomySammy
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Re: 4 weeks to the day [Re: LemurLemur]
    #24846591 - 12/14/17 08:42 PM (7 years, 1 month ago)

I'm sorry but this is only my second attempt


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InvisibleTheMilkMan84
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Re: 4 weeks to the day [Re: ShroomySammy]
    #24846593 - 12/14/17 08:43 PM (7 years, 1 month ago)

Quote:

ShroomySammy said:
How long would you let them go for?




I am by no means an expert, but usually when I get bacterial jars that are pinning early I try to let them colonize for another day or 2, then I’ll birth them regardless. Not sure what the best way is, but like Lemur says you can fruit them now & they’ll be fine


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Edited by TheMilkMan84 (12/14/17 08:45 PM)

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OfflineShroomySammy
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Re: 4 weeks to the day [Re: TheMilkMan84]
    #24846595 - 12/14/17 08:46 PM (7 years, 1 month ago)

Quote:

TheMilkMan84 said:
Quote:

ShroomySammy said:
How long would you let them go for?




I am by no means an expert, but usually when I get bacterial jars that are pinning early I try to let them colonize for another day or 2, then I’ll birth them regardless. Not sure what the best way is, but like Lemur says you can fruit them now & they’ll continue to colonize.



Thank you very much like I said this is only my second attempt


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OfflineShroomySammy
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Re: 4 weeks to the day [Re: LemurLemur]
    #24846600 - 12/14/17 08:48 PM (7 years, 1 month ago)

Quote:

LemurLemur said:
:megacrankey:......



I'll take that as a no sorry I'm still new to this


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InvisibleLemurLemur
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Re: 4 weeks to the day [Re: ShroomySammy]
    #24846612 - 12/14/17 08:54 PM (7 years, 1 month ago)

No you can eat them just fine:megacrankey:


Fruit them now cause a few days during pinning is a huge amount of time at that period.


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(when my data is fast play Lemur in chess at chess.com)[ [gradient:#D40B29,#18C418]Any1 expecting a trade from me i havent forgot about you pinky promise, i fr promise shits just shit rt now[/gradient]

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OfflinePrimalSoup
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Re: 4 weeks to the day [Re: ShroomySammy]
    #24846613 - 12/14/17 08:55 PM (7 years, 1 month ago)

Quote:

ShroomySammy said:



Here are some pictures 4 different jars




They're ready to fruit and so they're fruiting.  The part of the fully colonized sub that's fruiting doesn't know there's more sub sitting there, so for whatever reason they didn't churn through all of it.  Could be moisture or could be bacteria - looks like ropy myc going down on the bare areas from here which could mean...something... :shrug:


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OfflineCakewonder
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Re: 4 weeks to the day [Re: PrimalSoup]
    #24846630 - 12/14/17 09:03 PM (7 years, 1 month ago)

Quote:

ShroomySammy said:



Here are some pictures 4 different jars




One way to find bacteria is to smell the jars i believe, at least thats how Ive found it. Im pretty darn sure thats the way...


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OfflineCakewonder
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Re: 4 weeks to the day [Re: LemurLemur]
    #24846647 - 12/14/17 09:13 PM (7 years, 1 month ago)

Quote:

LemurLemur said:
How'd you prep yo sub? Sounds like bacterial sub.

Light is not a pinning trigger




Last, but not by any means least is exposure to light. Light does much more than just tell the mushrooms which way to grow. There are mechanisms in the light that stimulate the formation of hyphal knots as well, and light at the higher end of the spectrum(blue) definitely, absolutely stimulate more hyphal knots(which grow into primordia, which then morph into pins)

- RR

Well he say it is. Well now I know to always include the light in the fruiting part of the PF TEK as important. :smile: Thanks for telling me off, otherwise I wouldnt have found this:)


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OfflineCakewonder
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Re: 4 weeks to the day [Re: Cakewonder]
    #24846656 - 12/14/17 09:17 PM (7 years, 1 month ago)

By my listing them in order of importance, don't mistakenly think that minimizes the ones down the list. You don't want to increase FAE until full colonization. That indicates full colonization is the most important, so there is no conflict there. It's all in how the sentence is worded. All the pinning triggers need to be harmonized for best results.
RR

This is what ive always thought and how I practiced:)

Good luck to you OP, fruit that substrate now and dont forget the damn light:)


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InvisibleLemurLemur
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Re: 4 weeks to the day [Re: Cakewonder]
    #24846676 - 12/14/17 09:23 PM (7 years, 1 month ago)

Light helps when pinning is already triggered, it definitely makes bigger caps and gives vigorous growth.

It does nothing without the other triggers not being initiated.


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(when my data is fast play Lemur in chess at chess.com)[ [gradient:#D40B29,#18C418]Any1 expecting a trade from me i havent forgot about you pinky promise, i fr promise shits just shit rt now[/gradient]

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OfflineCakewonder
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Re: 4 weeks to the day [Re: LemurLemur]
    #24846706 - 12/14/17 09:42 PM (7 years, 1 month ago)

Well I remembered it wrong it was contams that could cause in vitro, not light. But damn im sure I read that here! Well..anyways, thanks for a good discussion!:smile:


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OfflineShroomySammy
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Re: 4 weeks to the day [Re: Cakewonder] * 2
    #24846717 - 12/14/17 09:47 PM (7 years, 1 month ago)



All four in chamber now smells like mushies thank you all


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Offlinekeeno
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Re: 4 weeks to the day [Re: ShroomySammy]
    #24847096 - 12/15/17 04:24 AM (7 years, 1 month ago)

best of luck with that! :cookiemonster:

what's the tape over the holes for? I'm pretty sure that's going to alter the airflow through the SGFC and make it work sub-optimally
at this stage you're not worried about keeping contams out but optimising conditions for fruiting
and FAE and high humidity at this stage is your friend, I think the tape wont' help

apologies if I got the wrong end of the stick

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OfflineCakewonder
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Re: 4 weeks to the day [Re: keeno]
    #24847106 - 12/15/17 04:39 AM (7 years, 1 month ago)

you also need a light


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OfflineShroomySammy
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Re: 4 weeks to the day [Re: Cakewonder]
    #24847490 - 12/15/17 10:07 AM (7 years, 1 month ago)

I'm picking up a light today and the tape I already had it assembled


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OfflineShroomySammy
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Re: 4 weeks to the day [Re: ShroomySammy] * 2
    #24886279 - 01/02/18 10:45 PM (7 years, 15 days ago)

That grew up great



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Offlinekeeno
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Re: 4 weeks to the day [Re: ShroomySammy]
    #24886662 - 01/03/18 04:08 AM (7 years, 15 days ago)

good vibes :laugh:
:jackiechanofapproval:

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OfflineShroomySammy
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Re: 4 weeks to the day [Re: keeno] * 2
    #24899304 - 01/08/18 07:55 PM (7 years, 9 days ago)



Flush 3 doing great


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InvisibleBattyKodaS
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Re: 4 weeks to the day [Re: ShroomySammy]
    #24899921 - 01/09/18 03:33 AM (7 years, 9 days ago)

Congratulations! :rockon:


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OfflineCakewonder
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Re: 4 weeks to the day [Re: BattyKoda] * 1
    #24902074 - 01/09/18 10:38 PM (7 years, 8 days ago)

congratz!


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InvisibleMrRabbit-HMR
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Re: 4 weeks to the day [Re: Cakewonder]
    #24902127 - 01/09/18 11:29 PM (7 years, 8 days ago)

Good work! I'd wait until after veil breaks though to maximize harvest, else you're doing yourself out of yield.

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