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Ferather
Mycological
Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 4 months
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T-Gel agar [Test log]
#24841886 - 12/12/17 01:07 PM (6 years, 3 months ago) |
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Here we have a bacterial infected wooden peg, it's a bacillus sp:
I took a scraping from the new peg, the left one, 48 hours:
This is [Recipe B], just black tea leaves.
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Ferather
Mycological
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Posts: 6,325
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Re: T-Gel agar [Test log] [Re: Ferather]
#24842153 - 12/12/17 02:56 PM (6 years, 3 months ago) |
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Feel free to add your own results with using T-Gel. And any related questions. You can also use wood extracts, to get adapted growth on agar.
Measure then end pH, and use it like tea extract.
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Ferather
Mycological
Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 4 months
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Re: T-Gel agar [Test log] [Re: Ferather]
#24843970 - 12/13/17 01:52 PM (6 years, 3 months ago) |
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Here is today's images, south to south-east you can see it's penetrating the agar. Also the growth is different, thicker single mycelium, more penetrative.
3g produces populate growth, 2g produces push growth.
Edited by Ferather (12/13/17 01:58 PM)
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Ferather
Mycological
Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: T-Gel agar [Test log] [Re: Ferather]
#24844010 - 12/13/17 02:13 PM (6 years, 3 months ago) |
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Also I found that adding too much CaCO3 causes a precipitate with the agar and tea. Which is useful if you need to lower the carbon content level of the agar.
I suggest pH 7.5 water, and no higher to be ME equivalent. The above example is pH 8.4, with precipitate.
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Ferather
Mycological
Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 4 months
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Re: T-Gel agar [Test log] [Re: Ferather]
#24844122 - 12/13/17 03:02 PM (6 years, 3 months ago) |
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I polished over my T-Gel recipe to be more informative, and easier to use.
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Ferather
Mycological
Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 4 months
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Re: T-Gel agar [Test log] [Re: Ferather]
#24845837 - 12/14/17 02:14 PM (6 years, 3 months ago) |
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I will be adding wood pegs soon, the short and sweet of it, I am successful. Goes to show what a difference no sugar and no starch makes.
[Recipe B]: Pass, even with high pH precipitate.
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Adas
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Re: T-Gel agar [Test log] [Re: Ferather] 2
#24845914 - 12/14/17 02:45 PM (6 years, 3 months ago) |
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What if you partly coated the wood pegs with some thin plastic coating and stuck them into the agar so that the coated part would be in the Agar but right above it the wood would be accessible? Could give the myc a chance to escape bacteria, since myc goes up in the air a bit. Just an idea.
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Ferather
Mycological
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Posts: 6,325
Loc: United Kingdom
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Re: T-Gel agar [Test log] [Re: Adas]
#24845995 - 12/14/17 03:17 PM (6 years, 3 months ago) |
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And make a bridge you mean? Enough for the mycelium to climb up, then get wood at the other end.
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Ferather
Mycological
Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 4 months
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Re: T-Gel agar [Test log] [Re: Ferather]
#24846044 - 12/14/17 03:43 PM (6 years, 3 months ago) |
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I have also considered adding an inhibitor to the pegs cooking water. Something that mycelium can deal with, but bad for bacteria.
Ideally it should be something I can get at a shop.
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Ferather
Mycological
Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 4 months
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Re: T-Gel agar [Test log] [Re: Ferather]
#24847312 - 12/15/17 08:14 AM (6 years, 3 months ago) |
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Here are today's images, its becoming well fed over time. 99% uniform radial, beginning to populate better.
I will add pegs in a few days.
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Ferather
Mycological
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Posts: 6,325
Loc: United Kingdom
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Re: T-Gel agar [Test log] [Re: Ferather]
#24847320 - 12/15/17 08:19 AM (6 years, 3 months ago) |
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I will also be testing a frozen Agrocybe aegerita fruit tissue sample onto T-Gel, [Recipe B]. The black poplar is store bought, I will isolate adapted growth, if it works.
Normal recipe, pH 7.5 water in my case, cold tap microwaved.
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I have adjusted the T-Gel recipe for a better blend.
Edited by Ferather (12/15/17 03:02 PM)
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Ferather
Mycological
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Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 4 months
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Re: T-Gel agar [Test log] [Re: Ferather]
#24851891 - 12/17/17 02:20 PM (6 years, 3 months ago) |
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The Agrocybe aegerita tissue turned bacterial after 2.5 days, semi-expected. I will try a high pH wash with calcium carbonate and try again.
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Ferather
Mycological
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Posts: 6,325
Loc: United Kingdom
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Re: T-Gel agar [Test log] [Re: Ferather]
#24851894 - 12/17/17 02:21 PM (6 years, 3 months ago) |
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Here I am transferring a peg to [Recipe B], I am using pH 8.0 calcium water, to compare it. Same recipe, but now with added CaCO3, the colour and blending is different.
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Ferather
Mycological
Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 4 months
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Re: T-Gel agar [Test log] [Re: Ferather]
#24853193 - 12/18/17 08:05 AM (6 years, 3 months ago) |
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18 hours later, the peg has regenerated, and grown out, areas with high O2 colonization have grown the best. Also the pegs absorb soluble carbon and soluble nutrients from any media they are placed into.
The pegs do no regenerate when pathogenic bacteria is present (see post 1). It seems I was successful at cleaning the first time round.
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Ferather
Mycological
Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 4 months
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Re: T-Gel agar [Test log] [Re: Ferather]
#24856359 - 12/19/17 05:53 PM (6 years, 3 months ago) |
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The Tarragon is more interested in cellulose than phenol's alone (as carbon). It has started colonizing the T-Gel, but prefers the wooden peg.
Both the peg and mycelium will uptake solubles.
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Humbled
Local Budtender
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Re: T-Gel agar [Test log] [Re: Ferather]
#24862140 - 12/22/17 01:34 PM (6 years, 3 months ago) |
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I was looking at your recipe and wondering if green tea would be as good or better? Maybe in combination with black tea?
This is really cool. I am about to start cultivating again after some years of distraction and I want to start tinkering with your formulas. Thanks for posting this!
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Ferather
Mycological
Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 4 months
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Re: T-Gel agar [Test log] [Re: Humbled]
#24862203 - 12/22/17 02:04 PM (6 years, 3 months ago) |
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I will explain why I use black tea, and also point out I have never tried green:
Black tea is green tea that has been "oxidized", which turns it black-brown. Laccase, the enzyme that breaks down the phenol's, oxidizes them. Oxidizing the phenol's, causes them to decay, which is why black tea is very dark in comparison to green tea, which is more fresh.
Using black tea allows the mycelium to decay and feed at a better rate, because black tea has already oxidized. Lignicolous mycelium (wood loving), spend the phenol's on primary units and various enzymes.
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Here is lignicolous mold germinating and colonizing T-Gel (phenol agar).
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Ferather
Mycological
Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 4 months
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Re: T-Gel agar [Test log] [Re: Ferather]
#24864008 - 12/23/17 02:10 PM (6 years, 3 months ago) |
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Here is the Tarragon oyster tissue sample to [Recipe B] so far. I will adding wood pegs soon, for transfer purposes.
Edited by Ferather (12/23/17 05:18 PM)
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Ferather
Mycological
Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 4 months
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Re: T-Gel agar [Test log] [Re: Ferather]
#24864140 - 12/23/17 03:15 PM (6 years, 3 months ago) |
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I will be transferring pegs to spent black tea bags, which are essentially nutritional leaf substrates in breathable paper bags. The tea will be spent into 400g of pH 8.4 water (CaCO3, lime, chalk), using a horizontal tray and a clean fork.
Tea leaves naturally contain no starch, and trace sugars (3g per 100g dry), which is mostly lost. They are high in nitrogen-protein and other macro-micro nutrients, fiber and phenol's.
Here is Cubensis on spent tea + trace sucrose (Cubensis is not lignicolous).
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I germinated it on my cellulose alternative + sugar peg.
Dry spores on the tip of a flamed peg.
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Ferather
Mycological
Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 4 months
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Re: T-Gel agar [Test log] [Re: Ferather]
#24867551 - 12/25/17 11:28 AM (6 years, 3 months ago) |
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The peg to spent tea bag is working as intended, after 18 hours, normal speed and population. I will takes images in a few days, due to assembly condensate and my camera focus.
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