Home | Community | Message Board

Magic Mushrooms Zamnesia
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Injection Grain Bag   Mushroom-Hut Substrate Bags   Left Coast Kratom Buy Kratom Extract

Jump to first unread post Pages: < First | < Back | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | Next > | Last >
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
Trusted Cultivator
Re: The Biopsy Method: A different way of doing LC. [Re: bodhisatta]
    #24835745 - 12/09/17 11:34 AM (6 years, 3 months ago)

Anyway malt look darker because it's full of all sorts of good shit.

Proteins from the malt, free amino nitrogen, enzymes, sugars, etc..

The only difference between LC and agar is the agar. LC is known as broth by all the rest of science.

Extras: Filter Print Post Top
InvisibleMateja
 Unread Journal User Gallery


Registered: 07/14/16
Posts: 7,948
Loc: Here
Re: The Biopsy Method: A different way of doing LC. [Re: bodhisatta]
    #24835753 - 12/09/17 11:39 AM (6 years, 3 months ago)

Josex Tek says 1g for 500ml, mushboy confirmed this, and somegirl corrected me as well and said she uses 1g for 500ml :shrug:


--------------------
Cakes inside Water Tub

Extras: Filter Print Post Top
Invisiblevan hattonFacebookDiscord
Still a noob
 User Gallery


Registered: 11/23/14
Posts: 5,617
Loc: Michigan
Trusted Cultivator
Re: The Biopsy Method: A different way of doing LC. [Re: bodhisatta]
    #24835754 - 12/09/17 11:40 AM (6 years, 3 months ago)

Quote:

bodhisatta said:
Whos doing 0.2% we usually use 2% so 20grams into 1L





It's in op 1-2gs per 500ml.

Bod pcing hdpe for 15 minutes yay or nay


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you

Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
Trusted Cultivator
Re: The Biopsy Method: A different way of doing LC. [Re: van hatton]
    #24835766 - 12/09/17 11:43 AM (6 years, 3 months ago)

I use more :shrug:

Extras: Filter Print Post Top
InvisibleJHOVA
Post whore
Male User Gallery


Registered: 02/17/17
Posts: 4,727
Loc: Flag
Re: The Biopsy Method: A different way of doing LC. [Re: bodhisatta]
    #24835771 - 12/09/17 11:46 AM (6 years, 3 months ago)

If 1 gram works why waste the rest?


--------------------
πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
Trusted Cultivator
Re: The Biopsy Method: A different way of doing LC. [Re: JHOVA]
    #24835780 - 12/09/17 11:49 AM (6 years, 3 months ago)

Likely more mycelial mass, and intracellular energy reserves. Thats just a hunch based on what I know works for yeast propagation.

Yeast likes around 8% for growth aerobically to get the best growth potential.

Extras: Filter Print Post Top
InvisibleJHOVA
Post whore
Male User Gallery


Registered: 02/17/17
Posts: 4,727
Loc: Flag
Re: The Biopsy Method: A different way of doing LC. [Re: bodhisatta]
    #24835788 - 12/09/17 11:52 AM (6 years, 3 months ago)

Do you notice any increase in colonizations with a stronger broth?


--------------------
πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

Extras: Filter Print Post Top
InvisibleTheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
Re: The Biopsy Method: A different way of doing LC. [Re: JHOVA]
    #24835893 - 12/09/17 12:36 PM (6 years, 3 months ago)

Shit I had been using0.5-1g/500ml so my mycelium mass doesn't get to damn thick. The couple times I used 2g it was crazy thick and a bitch to get into the needle.


--------------------
JOIN THE POW WOW

Extras: Filter Print Post Top
InvisiblemushboyMDiscord
modboy
 User Gallery


Registered: 04/24/05
Posts: 32,654
Loc: where?
Trusted Cultivator
OG Cultivator
Re: The Biopsy Method: A different way of doing LC. [Re: bodhisatta]
    #24835897 - 12/09/17 12:38 PM (6 years, 3 months ago)

Quote:

bodhisatta said:
I use more :shrug:





i made a broth with 5gram of poorly powderized oats. colonized the same as the others. jars are colonizing as we speak:thumbup:
i also did 2g with unfiltered brf and it was so thick and nasty i couldnt even see the myc let alone take a decent pic.

mateah-just make some broths with different ratios and report your findings:shrug: so far i like ~1g per ~400-500ml.

Quote:

TheMadHatter420 said:
crazy thick and a bitch to get into the needle.




follow the tek and pour that thick nasty shit. colonizes hella quick.
youll see a white glob ontop of the grains after pouring. 2days later is myc explosion:eek:

Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
Trusted Cultivator
Re: The Biopsy Method: A different way of doing LC. [Re: TheMadHatter420]
    #24835900 - 12/09/17 12:38 PM (6 years, 3 months ago)

16gauge?

Extras: Filter Print Post Top
InvisibleTheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
Re: The Biopsy Method: A different way of doing LC. [Re: bodhisatta]
    #24835905 - 12/09/17 12:40 PM (6 years, 3 months ago)

No mine was so thick I had issues with the LC dispenser needles clogging with mycelium.


--------------------
JOIN THE POW WOW

Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
Trusted Cultivator
Re: The Biopsy Method: A different way of doing LC. [Re: TheMadHatter420]
    #24835907 - 12/09/17 12:41 PM (6 years, 3 months ago)

Glass shards in your jar and a shake really help

Extras: Filter Print Post Top
InvisibleFungus Mountain
Poke-N-Squirt
 User Gallery


Registered: 10/16/14
Posts: 1,188
Loc: Front row @ the Freakshow
Re: The Biopsy Method: A different way of doing LC. [Re: bodhisatta]
    #24835954 - 12/09/17 01:04 PM (6 years, 3 months ago)

I followed the tek as written, several times with the same results. Used 1g/500ml LME, no filter, just the metal lid flipped, poke and squirt per OP. Left to sit to one week, I didn't fuck with it at all (pic is posted, no stir aids). After one week, I could see healthy colony growth with no indications of bacteria. 2nd week LC was agitated twice daily. Healthy growth, and well dispersed by day 14 and ready to go.

I'm an LC noob, so take my results with a grain of sand. Just saying that I followed it as close as I could and it works like a champ! I'd recommend that everyone give it a shot as written before adding their own flair to it. The results are inspiring.

:awesantabig:


--------------------
β€œUntil they became conscious they will never rebel, and until after they have rebelled they cannot become conscious.”
― George Orwell, 1984

"If you can't explain it simply, you don't understand it well enough."
― Albert Einstein

Extras: Filter Print Post Top
InvisiblemushboyMDiscord
modboy
 User Gallery


Registered: 04/24/05
Posts: 32,654
Loc: where?
Trusted Cultivator
OG Cultivator
Re: The Biopsy Method: A different way of doing LC. [Re: Fungus Mountain]
    #24835966 - 12/09/17 01:08 PM (6 years, 3 months ago)

the cleaner your cultures the better your LC. ive noticed what i thought was 'clean enough' for grains might not be that clean after all.:facepalm3:

Extras: Filter Print Post Top
Invisiblemicelio
Song of Silence
 User Gallery

Registered: 04/22/14
Posts: 1,547
Re: The Biopsy Method: A different way of doing LC. [Re: mushboy]
    #24836102 - 12/09/17 02:19 PM (6 years, 3 months ago)

:whathesaid:


--------------------
     

Extras: Filter Print Post Top
InvisibleTheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
Re: The Biopsy Method: A different way of doing LC. [Re: bodhisatta]
    #24836191 - 12/09/17 03:18 PM (6 years, 3 months ago)

Glass shards didn't work so well for me, but 3 glass marbles seem to shred it for me, for some reason. I guess their mass assists in a stronger swirling motion or some shit, lol.


--------------------
JOIN THE POW WOW

Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
Trusted Cultivator
Re: The Biopsy Method: A different way of doing LC. [Re: TheMadHatter420]
    #24836194 - 12/09/17 03:19 PM (6 years, 3 months ago)

I broke a pint glass some pieces are marble sized at least.

Extras: Filter Print Post Top
InvisibleTheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
Re: The Biopsy Method: A different way of doing LC. [Re: mushboy]
    #24836202 - 12/09/17 03:21 PM (6 years, 3 months ago)

Quote:

mushboy said:
the cleaner your cultures the better your LC. ive noticed what i thought was 'clean enough' for grains might not be that clean after all.:facepalm3:





Yea those will only really work for cakes, lol. I have been using agar for grains, and LC for cakes. LC is not something I need, but I make it to learn a new skill, and figure why not put it to use.


@Bod - I used a cracked half pint jar. Broke it up with a hammer and put 3 pieces in my jars. Not sure if the fractures just left the edges to dull or what, but hell marbles work. I guess it is possible my pieces were to small, about the size of a nickel.


--------------------
JOIN THE POW WOW

Extras: Filter Print Post Top
Invisiblesomegirl
Registered: 08/28/17
Posts: 197
Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24836729 - 12/09/17 08:36 PM (6 years, 3 months ago)

There was some discussion earlier in this thread about smaller gauge needles.  Just FYI, blunt 25GA (1.5") is too small, you can't squirt at all.  22Ga 1" sharps squirt well, I have blunt 1.5" coming from amazon.

The 25Ga will be great for spores though, you can only get one drop out at a time, slowly.

Extras: Filter Print Post Top
Invisiblevan hattonFacebookDiscord
Still a noob
 User Gallery


Registered: 11/23/14
Posts: 5,617
Loc: Michigan
Trusted Cultivator
Re: The Biopsy Method: A different way of doing LC. [Re: somegirl]
    #24838012 - 12/10/17 04:15 PM (6 years, 3 months ago)

Lc 13 days in. Been swirling for about 4 days


Lc poured over rye five days ago shaken 1.5 days ago. Looks weird I've never messed with LCS any thoughts?



--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you

Extras: Filter Print Post Top
Jump to top Pages: < First | < Back | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | Next > | Last >

Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Injection Grain Bag   Mushroom-Hut Substrate Bags   Left Coast Kratom Buy Kratom Extract


Similar ThreadsPosterViewsRepliesLast post
* Storing an LC needle. NewbieS 850 3 03/12/06 02:58 AM
by skeletor
* LC extraction for inoculation>? cannabisg 858 6 12/14/06 09:38 PM
by cannabisg
* LC needle redshadow 417 2 04/03/06 08:35 PM
by trauma47645
* LC lids faceyneck 4,764 11 02/11/07 09:09 AM
by agar
* Help Finding LC Jar Tek splifner180 1,233 6 05/18/06 01:05 PM
by kilroy
* Sterile Syringe for LC JoelLoveland5 2,285 9 02/23/07 08:22 AM
by Sillicybin
* LC question..kind of. inoculatedGreif 471 1 05/04/06 12:41 PM
by skeletor
* Buckaroo Bulk Nugget Tek - grain to bulk in one sealed container
( 1 2 all )
buckaroobanzai 20,095 23 09/12/06 04:27 AM
by fingers

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
128,264 topic views. 20 members, 117 guests and 102 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.03 seconds spending 0.01 seconds on 16 queries.