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InvisiblehamloafS
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Re: Official Caerulescens cult thread♤ [Re: TheMadHatter420] * 2
    #25147484 - 04/18/18 09:50 AM (10 months, 29 days ago)

They grow in sunny locations, prefer muddy orangish brown soils with lots of woody debris.  That tells us they like lots of light.  As far as bulk substrate woody soils can be compensated for by using straw, and mulch. 

Opted to use pine mulch, because this species is found growing in abundance in Georgia where a lot of pine trees grow.  Straw will be added for texture, and encouragement of a woody debris like substrate.  Coir will cover brownish soil department (straw/coir).  They fruit well in the low to mid 70's, as well.


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InvisibleTheMadHatter420
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Re: Official Caerulescens cult thread♤ [Re: hamloaf]
    #25147492 - 04/18/18 09:55 AM (10 months, 29 days ago)

I cant wait to see what you get.


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Re: Official Caerulescens cult thread♤ [Re: TheMadHatter420]
    #25193388 - 05/09/18 02:19 PM (10 months, 8 days ago)


Three months later this quart is still pushing up fruit


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Re: Official Caerulescens cult thread♤ [Re: lewp] * 1
    #25208193 - 05/16/18 04:33 PM (10 months, 1 day ago)

Tub in my backyard signaling it's hunting season.


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InvisiblehamloafS
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Re: Official Caerulescens cult thread♤ [Re: lewp]
    #25208566 - 05/16/18 07:31 PM (10 months, 21 hours ago)

Very nice, lewp!!  :super:


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OfflineHippiebabs
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Re: Official Caerulescens cult thread♤ [Re: hamloaf]
    #25382152 - 08/11/18 07:38 PM (7 months, 6 days ago)

Quote:

hamloaf said:
Here goes those jars of sterilized, supplemented, organic pine mulch 3 days out from inoculation.  :smile:

 




Super glad to see that. Last year when I took some sprints and tried to inoculate WBS, I uploaded pics and was told it was just mold and to just throw everything outside cuz I probably have better luck. Wasn't very encouraging at all. But now, I have this jar of mycelium that has just been suspended and honey water for about a year and didn't know what to try and do next.



Obviously I need to get syringes. And I had been throwing around the idea of gathering red clay and pine straw and some mulch and mixing it all together and doing it that way. obviously I would pressure cook to sterilize before I put any of the mycelium in it.

I also wanted an opinion on the darker spots inside of it. Are these contaminants? Remember this is a Year's growth. the air was a few of them to start with, and now there's more, but none of them are any bigger than the ones you see and have not gotten bigger for a very long time.


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OfflineBrain Bulb
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Re: Official Caerulescens cult thread♤ [Re: LemurLemur]
    #25634433 - 11/23/18 02:46 PM (3 months, 27 days ago)

Hey guys!  Wondering if any of you are still working on Caerulescens grows?  I have a few SB’s, 3 T3 clones and 2 T5’s from a wild print I took this summer.


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Offlinemoricz
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Re: Official Caerulescens cult thread♤ [Re: Brain Bulb]
    #25679196 - 12/14/18 04:56 PM (3 months, 5 days ago)

:creepylurker:


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InvisibleNimph
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Re: Official Caerulescens cult thread♤ [Re: moricz]
    #25679495 - 12/14/18 07:31 PM (3 months, 5 days ago)

:popcorn: :takingnotes:


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OfflineBrain Bulb
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Re: Official Caerulescens cult thread♤ [Re: Nimph]
    #25682849 - 12/16/18 01:19 PM (3 months, 4 days ago)

Already inoculated two jars of rye seed with my clone LC and they’re caking up with myc! 

Here’s one syringe of the LC and pics of GS:



I have 2 more 1/2 pint jars of LC, can I spread outside in the winter or will it kill the myc?


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Re: Official Caerulescens cult thread♤ [Re: Brain Bulb]
    #25764405 - 01/24/19 12:45 AM (1 month, 27 days ago)

Sorry for the bump, but I wanna watch this one... :takingnotes:


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OfflineBrain Bulb
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Re: Official Caerulescens cult thread♤ [Re: straightedge]
    #25765507 - 01/24/19 03:43 PM (1 month, 27 days ago)

Unfortunately I didn’t have any luck producing fruits on my previous grows and turned my focus to cubes for a while.  Also had a stretch of contaminated batches of agar so I was dealing with mold and bacteria but now I’m back on track.:-)

Just getting back to trying my hand at Caerulescens again and have a new phenotype I came across in my experiments on agar that is looking promising. (I will post pics at the bottom)

This particular phenotype came from me experimenting with P. Caerulescens and it’s resistance to mold.  A while back I swabbed a contaminated plate with spores from a wild print I took this fall.  I let the plate grow until it was covered in green mold except for where the Caerulescens spores were germinating.  As I was hoping, the spores started to resist the mold and made a visible defensive barrier.



I very carefully took a small transfer from the center of the area the Caerulescens spores had germinated.



I was also experimenting with different types of agar during this time.  (Definitely too much going on at once so I’ve slowed down significantly).  This is T2 of the experiment on DFA.



Was looking like I had got away from the mold in just two xfers but it ended up coming back.  However I turned this into something I could learn more from and started a second experiment on agar. (Not going in depth on X2 at the moment, but I will).  This is the plate I took my T3’s from.



This is where I started to have issues with contaminated batches of agar which I believe was due to damaged caps on my media bottles and some of the agar recipes I was trying. 

I have since got new media bottles, went back to a standard LME agar recipe (7g agar -> 7g LME -> 400mL distilled water) and contamination issues appear to have stopped.  Unfortunately I don’t have pics of my T4’s and T5’s because I was focused on getting clean plates of agar.

That brings me to T6 which was on LME agar and is the phenotype I mentioned at the beginning of this post.

Pics of T6:


Finally felt good enough to take it to grain and transferred to rye grass seed on 1/19.

GS jar as of 1/23 which is where I’m currently at. 



This phenotype is acting similar to how Panelaeous does on grain.  Still trying to figure out what substrate to try this time.  Right now I’m thinking about a mix of coir, hwfp and dried/sterilized red clay.

Other subs I’ve tried so far:  CV, straight coir, coir:hwfp and straight hwfp.

I’m open to feedback or any suggestions!


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Invisiblestraightedge
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Re: Official Caerulescens cult thread♤ [Re: Brain Bulb]
    #25770794 - 01/26/19 10:33 PM (1 month, 24 days ago)

Quote:

Brain Bulb said:
Other subs I’ve tried so far:  CV, straight coir, coir:hwfp and straight hwfp.





Does this mean you've tried fruiting with these substrates? What were your spawn rates, and generally your fruiting protocol?

At what point are you marking it down as a fail?


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Offlinespitzenkorper
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Re: Official Caerulescens cult thread♤ [Re: straightedge]
    #25859050 - 03/07/19 07:43 PM (15 days, 20 hours ago)

Im keep this thread alive yall...

Ps. caerulescens from Cherokee County GA
day 10, temps ~22C (20-24):



agar>GWLC>WBS
10cc GWLC : 4qt WBS




agar>GWLC> "mix"
(~2:2:2:1:1++ wbs : fir shavings : coir : coffee : oat bran + oyster + gypsum)
10cc GWLC : 4-5qt "mix"

more to come shortly...


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Edited by spitzenkorper (03/08/19 01:07 AM)


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OfflineBrain Bulb
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Re: Official Caerulescens cult thread♤ [Re: straightedge]
    #25859628 - 03/08/19 12:04 AM (15 days, 16 hours ago)

Quote:

straightedge said:
Quote:

Brain Bulb said:
Other subs I’ve tried so far:  CV, straight coir, coir:hwfp and straight hwfp.





Does this mean you've tried fruiting with these substrates? What were your spawn rates, and generally your fruiting protocol?

At what point are you marking it down as a fail?





Sorry just saw this.  Yes I have tried fruiting with these substrates with no luck.  I wasn’t as focused on conditions during my first few runs and I considered it a fail when the subs started showing signs of contamination which took a month or longer on a few. 

I have 3 new runs going now based off of @CaptainFuture’s Weilii tek that are looking good so far.  Have a grow log going so I’ll have better documentation going forward. —> https://www.shroomery.org/forums/showflat.php/Number/25798110


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OfflineBrain Bulb
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Re: Official Caerulescens cult thread♤ [Re: spitzenkorper]
    #25859637 - 03/08/19 12:06 AM (15 days, 16 hours ago)

Quote:

spitzenkorper said:
Im keep this thread alive yall...

Ps. caerulescens from Cherokee County GA
day 10:



agar>GWLC>WBS
10cc GWLC : 4qt WBS




agar>GWLC> "mix"
(~2:2:2:1:1++ wbs : fir shavings : coir : coffee : oat bran + oyster + gypsum)
10cc GWLC : 4-5qt "mix"

more to come shortly...




Nice!  Have you had any luck with the prints I sent you?


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Offlinespitzenkorper
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Re: Official Caerulescens cult thread♤ [Re: Brain Bulb]
    #25859722 - 03/08/19 12:59 AM (15 days, 15 hours ago)

Yeah BB, thank you! we're still workin on those, everything's on agar, but the only heat in my house is in my grow room, none in the lab, so everything moves slowwww brrr. we kinda just follow the flow of what looks ready to come out n play.
we should have some work from your samples comin out soon, I think we put the "summer" sample away for a later date, but working a few plates from the "winter" sample.

its just getting started ova here, we've now sourced several different samples of SE USA caerulescens, eventually we'll get a few all runnin at the same time.

we gon' be rrreal good friends, caeru n I, yes we are.

using the lc, we can test various substrates & conditions, compare results

working on a full proper grow log, will post in due time

if anyone has any genetics from mexico or elsewhere in latin america that they are willing to donate or share, itd be much appreciated. got lots to trade, holla!


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Re: Official Caerulescens cult thread♤ [Re: spitzenkorper]
    #25863061 - 03/09/19 02:04 PM (14 days, 2 hours ago)

SB #1 - Day 13 since cased.  Casing is not colonizing but I’m starting to see growth on the surface.  Think it needs more FAE so I’m leaving the lid off for the day.  Saw a recent post from Roger Rabbit stating that myc grows around the casing.  Hopefully this is what I’m seeing.



Myco bag #1 - Day 11 since cased.  It’s had more FAE than SB#1.  Had a lot of moisture in the bottom of the bag so there have been a couple days, off and on, where I’ve left the bag open.



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Re: Official Caerulescens cult thread♤ [Re: Brain Bulb]
    #25863609 - 03/09/19 06:11 PM (13 days, 22 hours ago)

Nice! what is the casing layer, cactus soil?

this wbs bag is bout colonized. we'll probably let it go another week or so to consolidate further, but then what idk. was thinking just case the grains, keep it simple, should get at least a few fruits right? but while drifting to sleep the other night, thought popped up to expand.

-should we case the grains and just go?
-should we prep some bulk sub, then
      -dump colonized grains into bulk sub?
      -dump bulk sub into colonized grains?

probably would just keep things in the bags if were going to expand, as moving both grain spawn and bulk subs to a new separate container is just adding an extra step for contams to enter.

it could be informative to see how just cased grains compare to bulk sub mix tho

we just dumped some oyster spawn into a bulk bag of coffee-straw, but it looks like it going green. so thats discouraging. i fear this place is just too old and moldy to open bags for transfers, can only get away with quick move precision of forceps & syringes.


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OfflineBrain Bulb
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Re: Official Caerulescens cult thread♤ [Re: spitzenkorper]
    #25866917 - 03/11/19 04:00 AM (12 days, 13 hours ago)

Quote:

spitzenkorper said:
Nice! what is the casing layer, cactus soil?

this wbs bag is bout colonized. we'll probably let it go another week or so to consolidate further, but then what idk. was thinking just case the grains, keep it simple, should get at least a few fruits right? but while drifting to sleep the other night, thought popped up to expand.

-should we case the grains and just go?
-should we prep some bulk sub, then
      -dump colonized grains into bulk sub?
      -dump bulk sub into colonized grains?

probably would just keep things in the bags if were going to expand, as moving both grain spawn and bulk subs to a new separate container is just adding an extra step for contams to enter.

it could be informative to see how just cased grains compare to bulk sub mix tho

we just dumped some oyster spawn into a bulk bag of coffee-straw, but it looks like it going green. so thats discouraging. i fear this place is just too old and moldy to open bags for transfers, can only get away with quick move precision of forceps & syringes.





Thanks!  These are my best looking attempts so far but I have yet to produce any fruits. 

I’ve been doing spawn to bulk recently and so far so good.  I’ve tried several other methods with no luck.  Even just casing GS.  The Caeru myc seems to metabolize grain quickly if you don’t have something mixed with it.

Method used so far is agar ~ grain ~ bulk sub ~ casing.  Spawning in jars then transferring to myco bag to colonize sub, then transferring to SB.  Let it recover 2-3 days before casing.  Probably going to do an LC for the next batch.

Sub I’ve been using is 30% Straw, 30% straw pellets, 20% pine shavings, 20% hpoo PC’d for 4 hours at 15 psi. 

These two are cased with peat, verm and perlite with CaCO3 to bring the pH up to 7 - 7.5.  I cased my 2nd SB with Miracle Grow Potting Mix which already has additives to raise the pH.  Going to see how it performs and if it does well, use it going forward.


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Edited by Brain Bulb (03/11/19 07:19 AM)


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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