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Shop: Myyco.com Golden Teacher Liquid Culture For Sale, Isolated Cubensis Liquid Culture For Sale   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

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InvisibleBrain Fart
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Registered: 12/19/07
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Accidentally froze my GLC...
    #24828394 - 12/05/17 04:58 PM (6 years, 4 months ago)

I just got a new mini fridge and it was set up a bit too high my grain liquid culture half froze with a nice big solid Chunk in the middle. There is still fluid on the sides and on the bottom but just wondering if this has happened to anyone else and if the myc will still be viable. It's cube mycelium.

:foreheadslap:


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Offlinetripdawg420
low life with no life
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Registered: 02/02/09
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Re: Accidentally froze my GLC... [Re: Brain Fart]
    #24828528 - 12/05/17 05:59 PM (6 years, 4 months ago)

only one way to find out:shrug:


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HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:

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InvisibleBrain Fart
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Re: Accidentally froze my GLC... [Re: tripdawg420]
    #24828540 - 12/05/17 06:05 PM (6 years, 4 months ago)

Lol I knew someone would say that. Newbie said he intentionally freezes his lc but a subtropical species wouldn't have a mechanism to prevent the cells from bursting (antifreeze)

I'm just wondering what others think. I could just remake it but I also don't want to shoot up 7 fresh jars with a dud:shrug:


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InvisibleBrain Fart
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Re: Accidentally froze my GLC... [Re: Brain Fart]
    #24828625 - 12/05/17 06:56 PM (6 years, 4 months ago)

Out of the 10 threads on this topic NOBODY reported back on results. Not even HippieChick or other veteran TC members.

I'm losing hope lol


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Offlinetripdawg420
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Re: Accidentally froze my GLC... [Re: Brain Fart]
    #24828735 - 12/05/17 07:53 PM (6 years, 4 months ago)

u could put it on agar few days u will know


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:

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InvisibleBrain Fart
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Registered: 12/19/07
Posts: 2,538
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Re: Accidentally froze my GLC... [Re: tripdawg420]
    #24828775 - 12/05/17 08:11 PM (6 years, 4 months ago)

Seems to be the consensus. If I make up agar I mite as well just clone a pin from the tub the glc grain jar was used to inoculate. I think I'll just end up pitching the glc.

At least I got to inoculate 7 quarts of rye with it before my fuckup:lol::shrug::spank:


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Offlinetripdawg420
low life with no life
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Registered: 02/02/09
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Re: Accidentally froze my GLC... [Re: Brain Fart]
    #24828789 - 12/05/17 08:18 PM (6 years, 4 months ago)

:thumbup:


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:

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InvisibleBrain Fart
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Registered: 12/19/07
Posts: 2,538
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Re: Accidentally froze my GLC... [Re: tripdawg420]
    #24828805 - 12/05/17 08:25 PM (6 years, 4 months ago)

Thx G:borat:


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OfflineThe Mycologist
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Registered: 05/06/16
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Re: Accidentally froze my GLC... [Re: Brain Fart]
    #24828838 - 12/05/17 08:42 PM (6 years, 4 months ago)

I think it may surprise you and survive


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"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
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InvisibleBrain Fart
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Registered: 12/19/07
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Re: Accidentally froze my GLC... [Re: The Mycologist]
    #24829389 - 12/06/17 07:43 AM (6 years, 4 months ago)

It may. I'll put a smidge on agar today.


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