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OfflineZyiadem
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Registered: 10/07/17
Posts: 392
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Re: Pasty Agar Tek [Re: somegirl]
    #24791416 - 11/18/17 11:25 AM (6 years, 3 months ago)

Never would have tried agar twice if I didn't find this.

Using a sterile needle and mycelium from a fresh clean cake, I stabbed the smallest bit of myc onto the needle and stabbed the center of my dish. Took a week or so (coldish where I'm at) I saw growth and now have 8 happy clean dishes growin :grin:


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I don't know shit.
And the more I know,
The more I realize it.


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Offlinepixelpopper
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Registered: 09/20/13
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Re: Pasty Agar Tek [Re: Zyiadem]
    #24792214 - 11/18/17 06:15 PM (6 years, 3 months ago)

I was going to be making up some more pasty plates soon and one thing I was wondering about adding food coloring - what if you get contam that is a similar color to the food coloring used? Wouldn't it be easier to spot on the light brownish color the mixture has before adding coloring?

All 3 of the agar dishes I put spore solution to are showing healthy growth that is starting to spread out. Looks like they should be good so far.

2 of the 3 with clone tissue look like they have some contam - one for sure looks like it has penicillium, but also some healthy growth which I was thinking about cutting away and transferring to a new dish but may not be worth the trouble. 2nd one has some weird looking "bubbled up" areas next to the tissue sample - not sure if its bacterial, contam or just my not so great too thick agar prep looking weird. One of the clone dishes looks good so far so hopefully it will come through.


Edited by pixelpopper (11/18/17 06:18 PM)


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InvisibleJHOVA
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Registered: 02/17/17
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Re: Pasty Agar Tek [Re: pixelpopper]
    #24793523 - 11/19/17 11:34 AM (6 years, 3 months ago)

reposted from this thread
Problem

Quote:


Should agar have a film on it? Am i being anal?

Made this last night

10 grams agar
7 potato flakes
Drop of karo
450 ml




Nasty shit



Thats only the first 3 plates i opened up. If its like the last couple batches then every plate is fucked.







Solution

Quote:

Thank you Josex for solving my problem.

My problem was water somehow sneaking inside under the paper towel through SFDS and MP tape during or after the PC cycle.

What he suggested was removing all media containers once the PC's pressure reaches zero.

Here are my results




No pooling no bacteria. Slight moisture on the walls i can live with.
Also lf note is that the paper towels are not damp or stuck to the MP tape.

Anyone having pooling issues try removing your no pours from the PC.:cheers:




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OfflineJFlint

Registered: 11/12/17
Posts: 115
Last seen: 5 years, 8 months
Re: Pasty Agar Tek [Re: Skatan6sic6]
    #24813674 - 11/29/17 06:13 AM (6 years, 2 months ago)

Anybody have any luck applying this technique and Pasty’s unmodified lid tek to this with jars? I’d like to try using half pint jars instead of the PP5 containers, but I hate jabbing holes in my nice plastic lids.


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InvisibleJHOVA
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Re: Pasty Agar Tek [Re: JFlint]
    #24813693 - 11/29/17 06:44 AM (6 years, 2 months ago)

Search wide mouth half pint in this thread


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OfflineJFlint

Registered: 11/12/17
Posts: 115
Last seen: 5 years, 8 months
Re: Pasty Agar Tek [Re: JHOVA]
    #24813758 - 11/29/17 07:25 AM (6 years, 2 months ago)

How do you limit searches to one thread?
EDIT: found it. Duh. Sorry.


Edited by JFlint (11/29/17 07:26 AM)


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Offlinetryptkaloids
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Re: Pasty Agar Tek [Re: JFlint]
    #24813789 - 11/29/17 07:44 AM (6 years, 2 months ago)

I use the regular mouth 1/4 pints with unmodded plastic lids all the time


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflineJFlint

Registered: 11/12/17
Posts: 115
Last seen: 5 years, 8 months
Re: Pasty Agar Tek [Re: tryptkaloids]
    #24814255 - 11/29/17 11:27 AM (6 years, 2 months ago)

Quote:

tryptkaloids said:
I use the regular mouth 1/4 pints with unmodded plastic lids all the time



Awesome. Care to elaborate? Do you take any precautions when PC’ing? Foil? Loose lids?
Do you tighten the lids after inoculation?
Any problems with condensation?


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Invisiblesomegirl
Registered: 08/28/17
Posts: 197
Re: Pasty Agar Tek [Re: JFlint]
    #24814264 - 11/29/17 11:31 AM (6 years, 2 months ago)

Quote:

JFlint said:
Quote:

tryptkaloids said:
I use the regular mouth 1/4 pints with unmodded plastic lids all the time



Awesome. Care to elaborate? Do you take any precautions when PC’ing? Foil? Loose lids?
Do you tighten the lids after inoculation?
Any problems with condensation?




https://www.shroomery.org/forums/showflat.php/Number/22771743#22771743

Edit, I see that is what started this, but the answers are there except condensation, which would be similar to any other lid will the same amount of GE - dependent on how loose or tight you close the lid.


Edited by somegirl (11/29/17 11:35 AM)


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OfflineZachsonpub
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Re: Pasty Agar Tek [Re: Pastywhyte]
    #24814607 - 11/29/17 02:03 PM (6 years, 2 months ago)

Hey, I wasn't positive where to ask, but this seems like the most relevant thread at the momentβ€”after some searching, I haven't found a mention of it specifically. I did use the search engine with a number of words/parameters.

Anyway, I wanted to know if anyone's tried PC'ing unmodified glad mini rounds. I have some at home, and even with the lid sitting on top, it makes flush contact with the containerβ€”so my concern would be that vacuum. Plus, if you're stacking, the lower rows get weighted down.

I only ask because it would be nice to keep the PP5s structurally sound.


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Invisiblesomegirl
Registered: 08/28/17
Posts: 197
Re: Pasty Agar Tek [Re: Zachsonpub]
    #24814616 - 11/29/17 02:07 PM (6 years, 2 months ago)

They will deform because of the vacuum.  The walls aren't firm enough at that temperature to handle it.  I've ruined a few containers by accident.

Edit: find som small screw top PP5s and you can leave them unmodified with the lid backed off a quarter turn or so.


Edited by somegirl (11/29/17 02:08 PM)


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OfflineZachsonpub
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Re: Pasty Agar Tek [Re: somegirl]
    #24814666 - 11/29/17 02:36 PM (6 years, 2 months ago)

Thanks for the quick response! That was my assumption..

Another question as I have had no success finding an answer: Can you PC mini rounds when they're empty? My end game being I'd pour them in a SAB.

Probably a dumb, turned around way of doing things, as this tek has you PC with the agar. I have it in my head that the agar provides the condensation that may keep the PP5 cool? Just wondering.. I'm new.


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Invisiblesomegirl
Registered: 08/28/17
Posts: 197
Re: Pasty Agar Tek [Re: Zachsonpub]
    #24814795 - 11/29/17 03:57 PM (6 years, 2 months ago)

You can PC them empty.  I'm not sure if you would get more or less condensation using them as pour plates.  Experiment:).


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InvisibleJHOVA
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Re: Pasty Agar Tek [Re: somegirl]
    #24814855 - 11/29/17 04:31 PM (6 years, 2 months ago)

the only thing about pouring in  pced pasty plates is having good enough sterile technique.

If you move too quickly to create a draft in the sab while pouring or have your hand directly over media you might contam your pasty plate.

Keep in mind pouring involves having your pasty upright and the beauty of pasty plates is being able to use them upside down or directly facing your sab holes on their side. Yes a sab SHOULDNT have anything floating down into open media but i think it certainly doesnt hurt taking that precaution.


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OfflineJFlint

Registered: 11/12/17
Posts: 115
Last seen: 5 years, 8 months
Re: Pasty Agar Tek [Re: JHOVA]
    #24817754 - 11/30/17 07:58 PM (6 years, 2 months ago)

I prepared twelve plates last night using 1/2 pint Kerr WM jars with unmodified plastic lids. Worked out great, however Ive got a bit of condensation on most of the jars, and even a bit of water pooling on the agar in a few of them. Any idea why the pooling may be occuring? Would this be more likely water entering from the PC, or just a lot of condensation?


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Offlinetombosley8
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Registered: 10/14/13
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Re: Pasty Agar Tek [Re: JFlint]
    #24817793 - 11/30/17 08:16 PM (6 years, 2 months ago)

just condensation. IT will go away after a week or so out of the pc but I always get condensation and have no issues. You can store them upside down and the water will pool on the lid instead of on the agar and the myc will be able to grow normally..

Even when stored upright the pooling doesn't cause me much problem at all unless dealing with clone tissue or sometimes it can cause my freshly transferred wedge slide around.:mad:

Usually the pooling just stays around the outside edge until it eventually gets absorbed into the agar. or evaporated.

My lids with sfd's or micropore tape allow that condensation to evaporate much faster but I don't mind it at all and love the unmodded lids so much.

kERR HALF PINTS AND UNMODDED BALL LIDS FOR THE WIN

Just don't tighten down the lids all the way while pcing and your set.

I also like to wrap the outside with saran after inoculating.



--------------------


Edited by tombosley8 (11/30/17 08:19 PM)


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OfflineJFlint

Registered: 11/12/17
Posts: 115
Last seen: 5 years, 8 months
Re: Pasty Agar Tek [Re: tombosley8]
    #24817807 - 11/30/17 08:24 PM (6 years, 2 months ago)

Quote:

tombosley8 said:
just condensation. IT will go away after a week or so out of the pc but I always get condensation and have no issues. You can store them upside down and the water will pool on the lid instead of on the agar and the myc will be able to grow normally..

Even when stored upright the pooling doesn't cause me much problem at all unless dealing with clone tissue or sometimes it can cause my freshly transferred wedge slide around.:mad:

Usually the pooling just stays around the outside edge until it eventually gets absorbed into the agar. or evaporated.

My lids with sfd's or micropore tape allow that condensation to evaporate much faster but I don't mind it at all and love the unmodded lids so much.

kERR HALF PINTS AND UNMODDED BALL LIDS FOR THE WIN

Just don't tighten down the lids all the way while pcing and your set.

I also like to wrap the outside with saran after inoculating.





Awesome, thanks for the feedback. I really like the Kerr jars. Theyve got much less "design" on the glass and offer way better visibility. I too like the unmodded lids too. Ive got a couple that have just a bit of wetness around the edges, and Im hoping the mycelium will still be able to colonize it okay.


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Offlinetombosley8
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Re: Pasty Agar Tek [Re: JFlint]
    #24817985 - 11/30/17 10:13 PM (6 years, 2 months ago)

they'll be fine.:thumbup:


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InvisiblePastywhyteMDiscord
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Re: Pasty Agar Tek [Re: tombosley8] * 5
    #24818530 - 12/01/17 09:02 AM (6 years, 2 months ago)

Wow its been 4 years, 269K views, 6000 responses, and a whole lotta agar work done. Thanks to the community for their support its been appreciated.

:rockon:


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OfflineSkatan6sic6
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Registered: 10/04/17
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
    #24819335 - 12/01/17 03:17 PM (6 years, 2 months ago)

Got a batch in the pc right now hopefully I didn't mess it up. I'm still working out my pc kinks on building pressure slowly. If I put it too low it won't build pressure. We shall see. If. It's wrong I'll try again. Thanks for the tek!


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