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Zyiadem
Smokes Catnip



Registered: 10/07/17
Posts: 392
Loc: Intn'l Waters
Last seen: 3 years, 2 months
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Re: Pasty Agar Tek [Re: somegirl]
#24791416 - 11/18/17 11:25 AM (6 years, 3 months ago) |
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Never would have tried agar twice if I didn't find this.
Using a sterile needle and mycelium from a fresh clean cake, I stabbed the smallest bit of myc onto the needle and stabbed the center of my dish. Took a week or so (coldish where I'm at) I saw growth and now have 8 happy clean dishes growin
-------------------- I don't know shit. And the more I know, The more I realize it.
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pixelpopper
Crap Artist


Registered: 09/20/13
Posts: 4,022
Loc: Dreamland
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Re: Pasty Agar Tek [Re: Zyiadem]
#24792214 - 11/18/17 06:15 PM (6 years, 3 months ago) |
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I was going to be making up some more pasty plates soon and one thing I was wondering about adding food coloring - what if you get contam that is a similar color to the food coloring used? Wouldn't it be easier to spot on the light brownish color the mixture has before adding coloring?
All 3 of the agar dishes I put spore solution to are showing healthy growth that is starting to spread out. Looks like they should be good so far.
2 of the 3 with clone tissue look like they have some contam - one for sure looks like it has penicillium, but also some healthy growth which I was thinking about cutting away and transferring to a new dish but may not be worth the trouble. 2nd one has some weird looking "bubbled up" areas next to the tissue sample - not sure if its bacterial, contam or just my not so great too thick agar prep looking weird. One of the clone dishes looks good so far so hopefully it will come through.
Edited by pixelpopper (11/18/17 06:18 PM)
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JHOVA
Post whore



Registered: 02/17/17
Posts: 4,727
Loc:
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reposted from this thread ProblemQuote:
Should agar have a film on it? Am i being anal?
Made this last night
10 grams agar 7 potato flakes Drop of karo 450 ml

Nasty shit

Thats only the first 3 plates i opened up. If its like the last couple batches then every plate is fucked.
SolutionQuote:
Thank you Josex for solving my problem.
My problem was water somehow sneaking inside under the paper towel through SFDS and MP tape during or after the PC cycle.
What he suggested was removing all media containers once the PC's pressure reaches zero.
Here are my results

No pooling no bacteria. Slight moisture on the walls i can live with. Also lf note is that the paper towels are not damp or stuck to the MP tape.
Anyone having pooling issues try removing your no pours from the PC.
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JFlint

Registered: 11/12/17
Posts: 115
Last seen: 5 years, 8 months
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Anybody have any luck applying this technique and Pastyβs unmodified lid tek to this with jars? Iβd like to try using half pint jars instead of the PP5 containers, but I hate jabbing holes in my nice plastic lids.
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JHOVA
Post whore



Registered: 02/17/17
Posts: 4,727
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Re: Pasty Agar Tek [Re: JFlint]
#24813693 - 11/29/17 06:44 AM (6 years, 2 months ago) |
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Search wide mouth half pint in this thread
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JFlint

Registered: 11/12/17
Posts: 115
Last seen: 5 years, 8 months
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Re: Pasty Agar Tek [Re: JHOVA]
#24813758 - 11/29/17 07:25 AM (6 years, 2 months ago) |
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How do you limit searches to one thread? EDIT: found it. Duh. Sorry.
Edited by JFlint (11/29/17 07:26 AM)
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
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Re: Pasty Agar Tek [Re: JFlint]
#24813789 - 11/29/17 07:44 AM (6 years, 2 months ago) |
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I use the regular mouth 1/4 pints with unmodded plastic lids all the time
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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JFlint

Registered: 11/12/17
Posts: 115
Last seen: 5 years, 8 months
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Quote:
tryptkaloids said: I use the regular mouth 1/4 pints with unmodded plastic lids all the time
Awesome. Care to elaborate? Do you take any precautions when PCβing? Foil? Loose lids? Do you tighten the lids after inoculation? Any problems with condensation?
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somegirl
Registered: 08/28/17
Posts: 197
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Re: Pasty Agar Tek [Re: JFlint]
#24814264 - 11/29/17 11:31 AM (6 years, 2 months ago) |
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Quote:
JFlint said:
Quote:
tryptkaloids said: I use the regular mouth 1/4 pints with unmodded plastic lids all the time
Awesome. Care to elaborate? Do you take any precautions when PCβing? Foil? Loose lids? Do you tighten the lids after inoculation? Any problems with condensation?
https://www.shroomery.org/forums/showflat.php/Number/22771743#22771743
Edit, I see that is what started this, but the answers are there except condensation, which would be similar to any other lid will the same amount of GE - dependent on how loose or tight you close the lid.
Edited by somegirl (11/29/17 11:35 AM)
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Zachsonpub
Friend



Registered: 03/02/16
Posts: 299
Last seen: 9 months, 15 days
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Hey, I wasn't positive where to ask, but this seems like the most relevant thread at the momentβafter some searching, I haven't found a mention of it specifically. I did use the search engine with a number of words/parameters.
Anyway, I wanted to know if anyone's tried PC'ing unmodified glad mini rounds. I have some at home, and even with the lid sitting on top, it makes flush contact with the containerβso my concern would be that vacuum. Plus, if you're stacking, the lower rows get weighted down.
I only ask because it would be nice to keep the PP5s structurally sound.
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somegirl
Registered: 08/28/17
Posts: 197
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They will deform because of the vacuum. The walls aren't firm enough at that temperature to handle it. I've ruined a few containers by accident.
Edit: find som small screw top PP5s and you can leave them unmodified with the lid backed off a quarter turn or so.
Edited by somegirl (11/29/17 02:08 PM)
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Zachsonpub
Friend



Registered: 03/02/16
Posts: 299
Last seen: 9 months, 15 days
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Re: Pasty Agar Tek [Re: somegirl]
#24814666 - 11/29/17 02:36 PM (6 years, 2 months ago) |
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Thanks for the quick response! That was my assumption..
Another question as I have had no success finding an answer: Can you PC mini rounds when they're empty? My end game being I'd pour them in a SAB.
Probably a dumb, turned around way of doing things, as this tek has you PC with the agar. I have it in my head that the agar provides the condensation that may keep the PP5 cool? Just wondering.. I'm new.
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somegirl
Registered: 08/28/17
Posts: 197
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You can PC them empty. I'm not sure if you would get more or less condensation using them as pour plates. Experiment:).
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JHOVA
Post whore



Registered: 02/17/17
Posts: 4,727
Loc:
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Re: Pasty Agar Tek [Re: somegirl]
#24814855 - 11/29/17 04:31 PM (6 years, 2 months ago) |
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the only thing about pouring in pced pasty plates is having good enough sterile technique.
If you move too quickly to create a draft in the sab while pouring or have your hand directly over media you might contam your pasty plate.
Keep in mind pouring involves having your pasty upright and the beauty of pasty plates is being able to use them upside down or directly facing your sab holes on their side. Yes a sab SHOULDNT have anything floating down into open media but i think it certainly doesnt hurt taking that precaution.
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JFlint

Registered: 11/12/17
Posts: 115
Last seen: 5 years, 8 months
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Re: Pasty Agar Tek [Re: JHOVA]
#24817754 - 11/30/17 07:58 PM (6 years, 2 months ago) |
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I prepared twelve plates last night using 1/2 pint Kerr WM jars with unmodified plastic lids. Worked out great, however Ive got a bit of condensation on most of the jars, and even a bit of water pooling on the agar in a few of them. Any idea why the pooling may be occuring? Would this be more likely water entering from the PC, or just a lot of condensation?
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 8 months, 24 days
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Re: Pasty Agar Tek [Re: JFlint]
#24817793 - 11/30/17 08:16 PM (6 years, 2 months ago) |
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just condensation. IT will go away after a week or so out of the pc but I always get condensation and have no issues. You can store them upside down and the water will pool on the lid instead of on the agar and the myc will be able to grow normally..
Even when stored upright the pooling doesn't cause me much problem at all unless dealing with clone tissue or sometimes it can cause my freshly transferred wedge slide around.
Usually the pooling just stays around the outside edge until it eventually gets absorbed into the agar. or evaporated.
My lids with sfd's or micropore tape allow that condensation to evaporate much faster but I don't mind it at all and love the unmodded lids so much.
kERR HALF PINTS AND UNMODDED BALL LIDS FOR THE WIN
Just don't tighten down the lids all the way while pcing and your set.
I also like to wrap the outside with saran after inoculating.
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Edited by tombosley8 (11/30/17 08:19 PM)
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JFlint

Registered: 11/12/17
Posts: 115
Last seen: 5 years, 8 months
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Quote:
tombosley8 said: just condensation. IT will go away after a week or so out of the pc but I always get condensation and have no issues. You can store them upside down and the water will pool on the lid instead of on the agar and the myc will be able to grow normally..
Even when stored upright the pooling doesn't cause me much problem at all unless dealing with clone tissue or sometimes it can cause my freshly transferred wedge slide around.
Usually the pooling just stays around the outside edge until it eventually gets absorbed into the agar. or evaporated.
My lids with sfd's or micropore tape allow that condensation to evaporate much faster but I don't mind it at all and love the unmodded lids so much.
kERR HALF PINTS AND UNMODDED BALL LIDS FOR THE WIN
Just don't tighten down the lids all the way while pcing and your set.
I also like to wrap the outside with saran after inoculating.

Awesome, thanks for the feedback. I really like the Kerr jars. Theyve got much less "design" on the glass and offer way better visibility. I too like the unmodded lids too. Ive got a couple that have just a bit of wetness around the edges, and Im hoping the mycelium will still be able to colonize it okay.
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 8 months, 24 days
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Re: Pasty Agar Tek [Re: JFlint]
#24817985 - 11/30/17 10:13 PM (6 years, 2 months ago) |
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they'll be fine.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Wow its been 4 years, 269K views, 6000 responses, and a whole lotta agar work done. Thanks to the community for their support its been appreciated.
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Skatan6sic6
Noob


Registered: 10/04/17
Posts: 10
Last seen: 6 months, 24 days
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
#24819335 - 12/01/17 03:17 PM (6 years, 2 months ago) |
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Got a batch in the pc right now hopefully I didn't mess it up. I'm still working out my pc kinks on building pressure slowly. If I put it too low it won't build pressure. We shall see. If. It's wrong I'll try again. Thanks for the tek!
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