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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: Cultivation General Discussion [Re: van hatton]
#24813226 - 11/28/17 10:28 PM (6 years, 3 months ago) |
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 I still like to noob needle injection ports lol.
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Alpineswift
Strangler


Registered: 10/14/16
Posts: 220
Last seen: 1 year, 7 months
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Re: Cultivation General Discussion [Re: van hatton]
#24813230 - 11/28/17 10:30 PM (6 years, 3 months ago) |
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Okay so I was looking at my grow area today and I wanted to give it a bit more light
I have to locations I am using to grow, one with some good natural sunlight And the other is the 2nd level of my closet
I have some 6500k bulbs and also a 2 2 foot T5 24w 6500k tubes
I don’t want to add any more than I need to, I will be doing the shoebox tek and I was wondering if something along these lines would work To maximize light ,

But instead of being on the opposite side of the window It will be on the 2nd level of my closet facing the wall and trapping light in between
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: Cultivation General Discussion [Re: bodhisatta] 1
#24813234 - 11/28/17 10:33 PM (6 years, 3 months ago) |
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 Inoculate the jars by taking off the lid in a sab(polyfil lids on left) or use SHIPs and noob needle everything.
Break the seal one time in a sab to inoculate the LC with the LC lid. I say if the seal is a hassle for you boo hoo get some man hands. I recently have been enjoying using the josex way to insulate with the needle biopsy. But usually use an agar wedge.
Tall jar with broken glass.
I think the pictures are self explanatory. Thats my easy tiltable no wet filter (unless you're a dumbass) LC tek
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: Alpineswift]
#24813241 - 11/28/17 10:38 PM (6 years, 3 months ago) |
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Im trying to figure out how to make syringes without a ship. A sterile "ship" ig.
That's what that picture was. One day after PC the controls are still good surprisingly not completely fucked yet.
Would be excellent if this works.
The idea is to trade out syringes like they do when they draw blood at the hospital.
My title is literally "the poke enthusiast" because the biopsy is working so well for me.
I use a set of flamed hemos to crack the seal.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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Munchauzen


Registered: 06/22/11
Posts: 14,343
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Re: Cultivation General Discussion [Re: van hatton]
#24813251 - 11/28/17 10:43 PM (6 years, 3 months ago) |
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Quote:
van hatton said: Im trying to figure out how to make syringes without a ship. A sterile "ship" ig.
That's what that picture was. One day after PC the controls are still good surprisingly not completely fucked yet.
Would be excellent if this works.
The idea is to trade out syringes like they do when they draw blood at the hospital.
My title is literally "the poke enthusiast" because the biopsy is working so well for me.
I use a set of flamed hemos to crack the seal.
ive been doing it for months. just take the lid off in a sab. i limit the jar to about 10 syringes. so i do 3 jars of lcs, 10 syringes each, 30 total. doesnt even take long. slow and smooth movements.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: Cultivation General Discussion [Re: Munchauzen]
#24813261 - 11/28/17 10:47 PM (6 years, 3 months ago) |
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I like tilting mine the mycelium tends to float so I can aspirate the syringe with thick LC a lot easier.
Ive thought about filling syringes with the lid off but I don't have a good way to tilt and pull the plunger. Or having to have my hands directly over top.
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: Munchauzen]
#24813273 - 11/28/17 10:53 PM (6 years, 3 months ago) |
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Everything I touch contams.. lol
I wouldn't feel confident doing it that way tbh. At least not at the moment. Maybe down the line when I can touch a petri without it contaminating.
Thus my reason for wanting something sterile to aspirate from. Not saying I won't use a ship I have an lc that almost done with a ship on it.
The only opening that my lid has during aspiration is a 16ga needle. (Works in theory not sure about practice)
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,982
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Re: Cultivation General Discussion [Re: bodhisatta]
#24813275 - 11/28/17 10:54 PM (6 years, 3 months ago) |
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I dislike plastic lids for G2G more than regular, for sure. But I also don't ever set my receiving or master lids down during G2G which is apparently the norm?
RE: syringes without a SHIP (which always give me trouble, even with an SFD they seem to form a vacuum on me, wtf?). I was toying with the idea of making some stupid little widget to hold the thing at a tilted angle. Or keeping the lid on and having 2 GE holes, but one is the size of the needle and covering it with removable whatever, e.g., taped on SFD, micropore/surgical tape, tight Poly, etc. Seems easy enough.
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Munchauzen


Registered: 06/22/11
Posts: 14,343
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Re: Cultivation General Discussion [Re: bodhisatta]
#24813278 - 11/28/17 10:54 PM (6 years, 3 months ago) |
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yeah i tilt them as well. its not difficult, just takes practice. ive been meaning to make a write up sometime soon. maybe ill do it with this jar tomorrow. ive also made plenty with my hand just over the jar and they were fine. a healthy dose of alcohol has done the trick for me on many syringes. but i do have a cheap little trick to tilt the jars and aspirate... will post soon 
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: Munchauzen]
#24813290 - 11/28/17 11:00 PM (6 years, 3 months ago) |
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@star eat clouds When did that become the norm.? I only set my master lid down because I plan to empty it.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,982
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Re: Cultivation General Discussion [Re: van hatton]
#24813295 - 11/28/17 11:03 PM (6 years, 3 months ago) |
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I was mainly referring to the receiving jars. I haven't read much at all on G2G technique and have only seen 1 video, I think, but I thought most tilted their receiving jar lids upside down somewhere before pouring in grain? And I think I've heard of people doing that with the master when they go to inoculate like 5 jars at once. I could be completely wrong, though.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
Last seen: 10 hours, 41 minutes
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Re: Cultivation General Discussion [Re: Munchauzen]
#24813312 - 11/28/17 11:10 PM (6 years, 3 months ago) |
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Quote:
Munchauzen said:
Quote:
van hatton said: Im trying to figure out how to make syringes without a ship. A sterile "ship" ig.
That's what that picture was. One day after PC the controls are still good surprisingly not completely fucked yet.
Would be excellent if this works.
The idea is to trade out syringes like they do when they draw blood at the hospital.
My title is literally "the poke enthusiast" because the biopsy is working so well for me.
I use a set of flamed hemos to crack the seal.
ive been doing it for months. just take the lid off in a sab. i limit the jar to about 10 syringes. so i do 3 jars of lcs, 10 syringes each, 30 total. doesnt even take long. slow and smooth movements.
I'm pretty sure he meant same needle in the jar. different syringes.
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Munchauzen


Registered: 06/22/11
Posts: 14,343
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Re: Cultivation General Discussion [Re: tryptkaloids]
#24813325 - 11/28/17 11:14 PM (6 years, 3 months ago) |
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Yes I understood that, but his design doesn't actually solve the issue of having a sterile syringe make physical contact with a non-sterile 'syringe-ship' thing. He mentioned swapping out syringes, well in that case the dispensing needle is exposed whenever its not attached to a syringe. so the best you could do is sanitize it, which is the same a ship.
so my suggestion is addressing the issue of having a sterile syringe contact a sanitary at best dispensing needle (or ship for that matter). by removing the lid you can aspirate from the jar with 0 physical contact.
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Cultivation General Discussion [Re: Munchauzen]
#24813342 - 11/28/17 11:20 PM (6 years, 3 months ago) |
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There's a way to solve all those issues. No tilt, no opening the lid to inoculate, no ship, no inserting needles through anything, and all this with rigurous sterility. I have all the materials to build this, it isn't complicated but I'm fucking lazy and also love the simplicity of pouring LC.
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Munchauzen


Registered: 06/22/11
Posts: 14,343
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Re: Cultivation General Discussion [Re: Josex]
#24813352 - 11/28/17 11:26 PM (6 years, 3 months ago) |
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do explain. does it require any kind of physical contact? sounds like a magic trick.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
Last seen: 10 hours, 41 minutes
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Re: Cultivation General Discussion [Re: Josex]
#24813354 - 11/28/17 11:28 PM (6 years, 3 months ago) |
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I'm eager for some writeups boys
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
Last seen: 10 hours, 41 minutes
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Re: Cultivation General Discussion [Re: tryptkaloids]
#24813356 - 11/28/17 11:29 PM (6 years, 3 months ago) |
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mushboy, do you have silicone? I love my single peice metal lids
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Variegated
Pressure cooker operator



Registered: 01/15/17
Posts: 820
Last seen: 2 years, 4 months
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Re: Cultivation General Discussion [Re: Josex]
#24813361 - 11/28/17 11:30 PM (6 years, 3 months ago) |
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Some one link me to a no fail lc tek. Every time i try it the liquid carmalizes..or i get no growth. Id really like to get into lc but i have failed at it every time..
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: Munchauzen]
#24813367 - 11/28/17 11:32 PM (6 years, 3 months ago) |
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Well the second you open a syringe or needle it's no longer sterile.. but you don't go wiping it down with iso that would be foolish.
The point is minimal surface area to reduce the chances of contams landing on the "ship thing" yes you leave exposed to trade syringes. your also in an sab so it shouldn't matter too much.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
Last seen: 10 hours, 41 minutes
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Re: Cultivation General Discussion [Re: Variegated]
#24813373 - 11/28/17 11:34 PM (6 years, 3 months ago) |
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have you tried pasty's EZLC or Josex's Biopsy method
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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