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OfflineThreads from God89
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Probability of Success using MS syringe inoculation directly to grain?
    #24812900 - 11/28/17 10:20 PM (3 years, 5 months ago)

Hello fello shroomers!!! :grin:

      I am looking to get opinions on the effectiveness of doing syringe ms inoculation straight to grain.  When this method is used, what is the success/failure ratio?  I know most experienced growers will always recommend starting ms with agar and then cleaning it up, and then doing up grain but is there ANYONE OUT THERE WHO HAS HAD SUCCESS USING MS DIRECTLY TO GRAIN?  I myself have had success with it so I am looking for other cultivators who use this method of inoculation. 

      I would like to be starting from agar but that is not an option right now.  So I am wondering, what are my chances of fruiting success using a ms syringe directly to wbs grain, and then combining with a bulk sub for a monotub?  50/50 chances of success?? 
 
      Thanks for your input!!!


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Re: Probability of Success using MS syringe inoculation directly to grain? [Re: Threads from God89]
    #24812918 - 11/28/17 10:26 PM (3 years, 5 months ago)

Plenty of people do that.  Agar is always an option.  Pastywhite has a good tek written up for making agar plates with gladware cups.  There are plenty of teks for making your own agar.


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Re: Probability of Success using MS syringe inoculation directly to grain? [Re: Threads from God89] * 1
    #24812920 - 11/28/17 10:28 PM (3 years, 5 months ago)

Ive had 3 out of 94 bags go down to contamination this year using that method. Thats an exact number because I keep detailed noted of experiments. Requires a ton of research and hard work though, but what doesn't?!?!


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Re: Probability of Success using MS syringe inoculation directly to grain? [Re: Helnak] * 1
    #24812923 - 11/28/17 10:29 PM (3 years, 5 months ago)

ive done it a few times. it works, its just not the best option. I would do a half pint jar of grains knock it up, then once fully colonized g2g to 4 or 5 quart grain jars


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OfflineThreads from God89
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Re: Probability of Success using MS syringe inoculation directly to grain? [Re: Ziran]
    #24812981 - 11/28/17 10:47 PM (3 years, 5 months ago)

Quote:

Gurdjieff said:
Ive had 3 out of 94 bags go down to contamination this year using that method. Thats an exact number because I keep detailed noted of experiments. Requires a ton of research and hard work though, but what doesn't?!?!




And you're using Multi-Spore syringe directly to grain?  Awesome, yeah I plan on doing three 5 quart spawn bags with an ms syringe so I am looking for reasons to be Optimistic!! Haha

Quote:

Ziran said:
ive done it a few times. it works, its just not the best option. I would do a half pint jar of grains knock it up, then once fully colonized g2g to 4 or 5 quart grain jars




  What if there is an unseen, unnoticed contam in the parent jar?  Can the transfer jars colonize seemingly fine until you bulk and then the hidden contam could wreak havoc?  Just wondering because I believe something similar just occurred with a mono I did.


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OfflineThreads from God89
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Re: Probability of Success using MS syringe inoculation directly to grain? [Re: Helnak]
    #24812988 - 11/28/17 10:51 PM (3 years, 5 months ago)

Quote:

Helnak said:
Plenty of people do that.  Agar is always an option.  Pastywhite has a good tek written up for making agar plates with gladware cups.  There are plenty of teks for making your own agar.




Thank you, I will definetly check those out!!!


--------------------
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Re: Probability of Success using MS syringe inoculation directly to grain? [Re: Threads from God89]
    #24813007 - 11/28/17 11:01 PM (3 years, 5 months ago)

Above 80-90% with any good syringe


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Re: Probability of Success using MS syringe inoculation directly to grain? [Re: Threads from God89]
    #24813027 - 11/28/17 11:05 PM (3 years, 5 months ago)

Quote:

Ziran said:
ive done it a few times. it works, its just not the best option. I would do a half pint jar of grains knock it up, then once fully colonized g2g to 4 or 5 quart grain jars



Quote:

Threads from God89 said:
Quote:

Gurdjieff said:
Ive had 3 out of 94 bags go down to contamination this year using that method. Thats an exact number because I keep detailed noted of experiments. Requires a ton of research and hard work though, but what doesn't?!?!




And you're using Multi-Spore syringe directly to grain?  Awesome, yeah I plan on doing three 5 quart spawn bags with an ms syringe so I am looking for reasons to be Optimistic!! Haha

Quote:

Ziran said:
ive done it a few times. it works, its just not the best option. I would do a half pint jar of grains knock it up, then once fully colonized g2g to 4 or 5 quart grain jars




  What if there is an unseen, unnoticed contam in the parent jar?  Can the transfer jars colonize seemingly fine until you bulk and then the hidden contam could wreak havoc?  Just wondering because I believe something similar just occurred with a mono I did.



Yes.


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OfflineThreads from God89
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Re: Probability of Success using MS syringe inoculation directly to grain? [Re: bodhisatta]
    #24814348 - 11/29/17 02:01 PM (3 years, 5 months ago)

Quote:

bodhisatta said:
Above 80-90% with any good syringe




Awesome!  Thanks!!


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Re: Probability of Success using MS syringe inoculation directly to grain? [Re: Threads from God89]
    #24816297 - 11/30/17 10:29 AM (3 years, 5 months ago)

So what is the best way to test colonized jars of grain for unseen contaminates?  Do you guys shake jars and test for recovery 24 hours before bulking??  I have seen where some growers don't do a shake a test.......is it best to shake jars to test for contams 24 hours before bulking?  Is that a good indicator of contams, a jar that DOES NOT recover from shaking?

    Thanks for the info guys, this last failed monotub I had really sucked, I really want to make sure I check all variables and am successful this time.....


--------------------
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    I Will Keep the Fire going,  and the Good Wolf fed.......

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Re: Probability of Success using MS syringe inoculation directly to grain? [Re: Threads from God89] * 1
    #24816363 - 11/30/17 11:03 AM (3 years, 5 months ago)

jars that do not recover from shakes are fucked. you can top fruit but thats about it besides tossing.

Quote:

Threads from God89 said:
I really want to make sure I check all variables and am successful this time.....




THAT plus your threads title gave me forest whitaker eye.


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Re: Probability of Success using MS syringe inoculation directly to grain? [Re: mushboy] * 1
    #24816365 - 11/30/17 11:04 AM (3 years, 5 months ago)

:whathesaid:


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Re: Probability of Success using MS syringe inoculation directly to grain? [Re: bodhisatta]
    #24816501 - 11/30/17 12:27 PM (3 years, 5 months ago)

You can knock up grain spawn you just need to be able to spot healthy jars..post pics along the way


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Re: Probability of Success using MS syringe inoculation directly to grain? [Re: bodhisatta]
    #24816526 - 11/30/17 12:41 PM (3 years, 5 months ago)

Quote:

mushboy said:
jars that do not recover from shakes are fucked. you can top fruit but thats about it besides tossing.

Quote:

Threads from God89 said:
I really want to make sure I check all variables and am successful this time.....




THAT plus your threads title gave me forest whitaker eye.




:laugh:...... So this is probably a kinda dumb question, but what is top fruiting?  Like putting a layer of coir on top of a jar of grain and fruiting it?


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Re: Probability of Success using MS syringe inoculation directly to grain? [Re: Threads from God89]
    #24816535 - 11/30/17 12:45 PM (3 years, 5 months ago)

Yep


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Re: Probability of Success using MS syringe inoculation directly to grain? [Re: cronicr]
    #24816536 - 11/30/17 12:46 PM (3 years, 5 months ago)

Right on, makes sense...... yeah I am at work at the moment, my home wifi is messed up right now so I can only post when im here ....:thumbdown:


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Re: Probability of Success using MS syringe inoculation directly to grain? [Re: Threads from God89]
    #24816621 - 11/30/17 01:35 PM (3 years, 5 months ago)

Does it usually result in fruits trying to top fruit an unrecovered grain jar?  So I just apply like a 1/4 inch of cooked coir, keep misted and then give fruiting conditions??  I have about 4 or 5 jars that I could try and top fruit.  Is it better to do that or just do up a few trays? 
       
      Thanks again!  :thumbup:    appreciate the advice!!


--------------------
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    I Will Keep the Fire going,  and the Good Wolf fed.......

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Re: Probability of Success using MS syringe inoculation directly to grain? [Re: Threads from God89]
    #24816646 - 11/30/17 01:44 PM (3 years, 5 months ago)

Every mono i have had this year that got trich before the first flush was ms to grain. I wont be doing it anymore no matter how lazy i feel. I had poor results but everyone is differant.


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Re: Probability of Success using MS syringe inoculation directly to grain? [Re: Threads from God89]
    #24816658 - 11/30/17 01:50 PM (3 years, 5 months ago)

Quote:

Variegated said:
Every mono i have had this year that got trich before the first flush was ms to grain. I wont be doing it anymore no matter how lazy i feel. I had poor results but everyone is differant.



Yep this is the result of not being able to tell a clean jar. ..it's not always easy
Quote:

Threads from God89 said:
Does it usually result in fruits trying to top fruit an unrecovered grain jar?  So I just apply like a 1/4 inch of cooked coir, keep misted and then give fruiting conditions??  I have about 4 or 5 jars that I could try and top fruit.  Is it better to do that or just do up a few trays? 
       
      Thanks again!  :thumbup:    appreciate the advice!!



Yep


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Re: Probability of Success using MS syringe inoculation directly to grain? [Re: Variegated]
    #24816740 - 11/30/17 02:31 PM (3 years, 5 months ago)

Damn......that sucks man. :frown:
Quote:

Variegated said:
Every mono i have had this year that got trich before the first flush was ms to grain. I wont be doing it anymore no matter how lazy i feel. I had poor results but everyone is differant.




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Admirer of Bushido....... Seeker of Truth, and Knowledge.

    I Will Keep the Fire going,  and the Good Wolf fed.......

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