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qqG
Aspiring Hobbyist


Registered: 06/11/17
Posts: 12
Last seen: 6 years, 10 months
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Re: Salvage contaminated Mycobag [Re: Snazz]
#24811322 - 11/28/17 09:32 AM (7 years, 1 month ago) |
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Quote:
TaderHoagie said: I'd go with GurdJieff. The work invested to save this grow would be better spent on starting from scratch.
Quote:
Snazz said: https://www.shroomery.org/forums/showflat.php/Number/23325820#23325820
It's not really any work. Early on, resources mean a lot more than after you blow out a ton of monos.
Early experience on learning the life cycle and habits. All are lessons 
Woke up this morning to a few quarter-sized green splotches and these "green flakes" around it. Pretty sure its trich. or some kind of mold
I'm going to go ahead and toss it. Start again fresh this Spring with the PF Tek. I really like this EQ strain. Very healthy rhizmorphic growth and fast(and I mean fast!) colonization. I saw this strain beginning to chew up sub within 3-4 days of colonization.
Even though i had issues with it in this Mycobag; Which Ive learned is common, regardless of strain if a MS/LC is used; Rather than Agar or grain transfer.
Correct me if Im wrong here, but the general consensus seems to be MS/LC--> PF Tek || Agar/Grain2Grain ----> Mycobags. They need a cleaner spawn or something right?
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Snazz
Polymath



Registered: 11/24/15
Posts: 1,586
Loc: Canada
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Re: Salvage contaminated Mycobag [Re: qqG]
#24811330 - 11/28/17 09:40 AM (7 years, 1 month ago) |
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Bags really need clean LC or a few good jars to pull off. Longer the colonization time, the more (non)sterile technique bites you in ass.
Shoeboxes are quite stellar at seeing how clean those pints really are. Big monos are less prep but one bit of green will be that much more costly.
Cheers 
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somegirl
Registered: 08/28/17
Posts: 197
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Re: Salvage contaminated Mycobag [Re: qqG]
#24811351 - 11/28/17 09:55 AM (7 years, 1 month ago) |
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Quote:
qqG said:
Quote:
TaderHoagie said: I'd go with GurdJieff. The work invested to save this grow would be better spent on starting from scratch.
Quote:
Snazz said: https://www.shroomery.org/forums/showflat.php/Number/23325820#23325820
It's not really any work. Early on, resources mean a lot more than after you blow out a ton of monos.
Early experience on learning the life cycle and habits. All are lessons 
Woke up this morning to a few quarter-sized green splotches and these "green flakes" around it. Pretty sure its trich. or some kind of mold
I'm going to go ahead and toss it. Start again fresh this Spring with the PF Tek. I really like this EQ strain. Very healthy rhizmorphic growth and fast(and I mean fast!) colonization. I saw this strain beginning to chew up sub within 3-4 days of colonization.
Even though i had issues with it in this Mycobag; Which Ive learned is common, regardless of strain if a MS/LC is used; Rather than Agar or grain transfer.
Correct me if Im wrong here, but the general consensus seems to be MS/LC--> PF Tek || Agar/Grain2Grain ----> Mycobags. They need a cleaner spawn or something right?
More like spore syringes only can reliably be used to inoculate for PF tek. LC works great for grain or PF Tek but has to be made from agar, not spores. All of the above are MS. MS does get used quite a bit to refer to spore syringes, but not the same thing. MS is multispore, which is true of everything other than an isolate. MS applies to everything other than a clone or isolate, although using agar narrows the genetics.
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qqG
Aspiring Hobbyist


Registered: 06/11/17
Posts: 12
Last seen: 6 years, 10 months
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Re: Salvage contaminated Mycobag [Re: somegirl]
#24863588 - 12/23/17 10:44 AM (7 years, 25 days ago) |
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Anyways, Ive got about 3 CC's of MS solution left to fiddle with, and of course a brand new Rye-berry Manure/Straw grow bag. I want to try and get better results this time so I'd greatly appreciate some tips and recommendations on what ive already learned from this thread:
- Incubate the bag after inoculation * At what temperatures exactly? I have a small fan-based space heater I could use to warm a space up if needed.
- Dont fuck with the bag after I inoculate it, place it in a warm, dark space and check up on it as needed. * I was thinking about putting the bag in a small transparent latched lid sterilite container. would this be OK? Does the bag need fresh air while germinating or will putting it in a warm area inside the sterilite container work?
- Dont use TOO much spore solution * How much would you guys recommend for a grow bag containing 3lb of substrate? I have roughly 3CC's Left. ALSO: Would shaking the bag after inoculation help speed up colonization time?
Edited by qqG (12/23/17 01:06 PM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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Re: Salvage contaminated Mycobag [Re: qqG]
#24863621 - 12/23/17 11:06 AM (7 years, 25 days ago) |
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3. Spores of active species are sold for microscopy use only so mycologists can ID mushrooms. Cultivation supplies are for legitimate legal growing. If you're going to misuse them to grow a controlled substance, keep it to yourself. Any post which mentions 'such and such' vendor, will be deleted and you'll receive an official warning. No one needs to know where you got your spores, kit, or other supplies.
Please leave where you got your stuff out. No need to throw vendors under the bus
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qqG
Aspiring Hobbyist


Registered: 06/11/17
Posts: 12
Last seen: 6 years, 10 months
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Re: Salvage contaminated Mycobag [Re: bodhisatta]
#24863639 - 12/23/17 11:16 AM (7 years, 25 days ago) |
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Quote:
bodhisatta said: 3. Spores of active species are sold for microscopy use only so mycologists can ID mushrooms. Cultivation supplies are for legitimate legal growing. If you're going to misuse them to grow a controlled substance, keep it to yourself. Any post which mentions 'such and such' vendor, will be deleted and you'll receive an official warning. No one needs to know where you got your spores, kit, or other supplies.
Please leave where you got your stuff out. No need to throw vendors under the bus
Absolutely, my mistake. It was never my intention to throw any one under the bus. thanks for the edit.
Could I get some input on my original questions though?
Edited by qqG (12/23/17 11:25 AM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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Re: Salvage contaminated Mycobag [Re: qqG]
#24863641 - 12/23/17 11:17 AM (7 years, 25 days ago) |
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All good. Just courtesy notice
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qqG
Aspiring Hobbyist


Registered: 06/11/17
Posts: 12
Last seen: 6 years, 10 months
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Re: Salvage contaminated Mycobag [Re: qqG]
#24865499 - 12/24/17 08:48 AM (7 years, 24 days ago) |
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Quote:
qqG said: Anyways, Ive got about 3 CC's of MS solution left to fiddle with, and of course a brand new Rye-berry Manure/Straw grow bag. I want to try and get better results this time so I'd greatly appreciate some tips and recommendations on what ive already learned from this thread:
- Incubate the bag after inoculation * At what temperatures exactly? I have a small fan-based space heater I could use to warm a space up if needed.
- Dont fuck with the bag after I inoculate it, place it in a warm, dark space and check up on it as needed. * I was thinking about putting the bag in a small transparent latched lid sterilite container. would this be OK? Does the bag need fresh air while germinating or will putting it in a warm area inside the sterilite container work?
- Dont use TOO much spore solution * How much would you guys recommend for a grow bag containing 3lb of substrate? I have roughly 3CC's Left. ALSO: Would shaking the bag after inoculation help speed up colonization time?
Id greatly appreciate some input on these questions
Inoculated about 2CC's yesterday, following sterile procedures. Put it in the above described container. Temps are roughly 68-72F inside the container. Now the hardest part, waiting! Still have about 1CC left, might try and make LC out of it, if thats even enough.
Hamloaf, not sure I feel comfortable working with agar yet. Lack of knowledge/funds Unless its relatively simple and inexpensive, then please enlighten me!!
Edited by qqG (12/24/17 08:59 AM)
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hamloaf
Q-dood ®©™√



Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 5 hours, 46 minutes
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Re: Salvage contaminated Mycobag [Re: qqG]
#24865506 - 12/24/17 08:54 AM (7 years, 24 days ago) |
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Take the spores to agar. Once a clean culture on agar is established use the wedge and transfer technique to inoculate sterilzed grains in jars. Use fully colonized grains spawn from jars to inoculate bags. If you don't have a flowhood skip the bag step all together and spawn to bulk spawn generated inside of jars.
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Cybin_man
Circle the Wagons


Registered: 05/02/17
Posts: 799
Loc: In the bathroom
Last seen: 4 years, 9 months
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Re: Salvage contaminated Mycobag [Re: qqG]
#24865696 - 12/24/17 10:33 AM (7 years, 24 days ago) |
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First of all you should have bought a rye berry spawn bag separate from a substrate bag IMO. I just got done with two a couple months ago. I bought 2 - 2 lb rye berry bags and I bought 2 - 10 lb compost/substrate bags. I noc’d up the two rye berry bags, I think I used 2 - 3 cc’s each, I can’t remember exactly. And I put my two bags of substrate in the fridge until I was ready to use them. Or u could wait and buy them later when your spawn is almost done colonizing.
Don’t put your bag inside a plastic container when colonizing. I just left mine in a spare bedroom on top of an old entertainment center (away from the floor), and I have a little space heater that kept the room between 70-80 degrees. Once your rye berries colonize 40% smash up the mycelium and mix it throughout the bag then it will finish colonizing faster. That’s kind of why you don’t want substrate in the same bag as your rye berries. Then once your rye berries are fully colonized, you can break up all your rye berry spawn and dump it straight into a bag of substrate, seal it with paper clips, and mix it up really good. Then I let that colonize fully before putting it in fruiting conditions. Put it under some lights or by a window with indirect sunlight @around 70-75 degrees.
You can also make a monotub and mix up all your spawn and substrate and grow out of the tub instead of the bag. With my two bags I got around a pound of dried mushrooms. I did a couple more like this also (grown in a bag) and did quite well. Just do your inoculation in a sterile environment and be as clean as possible. I never ran into contamination until my bags were done flushing.
-------------------- mushrooms + my morning jacket = awesome https://youtu.be/xkY4isMi2Zc
 
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hamloaf
Q-dood ®©™√



Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 5 hours, 46 minutes
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Re: Salvage contaminated Mycobag [Re: qqG]
#24865788 - 12/24/17 11:21 AM (7 years, 24 days ago) |
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Quote:
qqG said:
Quote:
qqG said: Anyways, Ive got about 3 CC's of MS solution left to fiddle with, and of course a brand new Rye-berry Manure/Straw grow bag. I want to try and get better results this time so I'd greatly appreciate some tips and recommendations on what ive already learned from this thread:
- Incubate the bag after inoculation * At what temperatures exactly? I have a small fan-based space heater I could use to warm a space up if needed.
- Dont fuck with the bag after I inoculate it, place it in a warm, dark space and check up on it as needed. * I was thinking about putting the bag in a small transparent latched lid sterilite container. would this be OK? Does the bag need fresh air while germinating or will putting it in a warm area inside the sterilite container work?
- Dont use TOO much spore solution * How much would you guys recommend for a grow bag containing 3lb of substrate? I have roughly 3CC's Left. ALSO: Would shaking the bag after inoculation help speed up colonization time?
Id greatly appreciate some input on these questions
Inoculated about 2CC's yesterday, following sterile procedures. Put it in the above described container. Temps are roughly 68-72F inside the container. Now the hardest part, waiting! Still have about 1CC left, might try and make LC out of it, if thats even enough.
Hamloaf, not sure I feel comfortable working with agar yet. Lack of knowledge/funds Unless its relatively simple and inexpensive, then please enlighten me!!
Don't use your spore solution to inoculate an LC with. That's asking for problems. Click the link below for an easy, comprehensive agar technique. https://www.shroomery.org/forums/showflat.php/Number/19208976#19208976
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