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OfflinecharlieXxXxX
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Registered: 03/29/04
Posts: 80
Loc: Earth, USA, WI, Madison
Last seen: 11 years, 9 months
Lion's mane wedge
    #2480386 - 03/29/04 05:10 PM (12 years, 8 months ago)


I have a few Lion's mane (FP Hericium erinaceus) wedges
on Hydrogen peroxided agar that I could send someone.

(I am uncertain on how one mails a wedge though. Do you
just cut a wedge and drop it in a baggie and mail it?)

I am interested in getting Maitake, Shiitake, Agaricus
subrufescens, Pleurotus eryngii or Hypsizygus ulmarius if
anyone has them.


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Anonymous

Re: Lion's mane wedge [Re: charlieXxXxX]
    #2480455 - 03/29/04 05:24 PM (12 years, 8 months ago)

I put a wedge in like a dime baggy inside a sandwhich baggy inside a pitri dish.
Labeled on the baggy and on the petri dish as well. Inside a bubble envelpe.


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OfflineAnnoA
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Re: Lion's mane wedge [Re: charlieXxXxX]
    #2480616 - 03/29/04 06:07 PM (12 years, 8 months ago)

I use a ziplock baggie in a bubble envelope.



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OfflinecharlieXxXxX
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Registered: 03/29/04
Posts: 80
Loc: Earth, USA, WI, Madison
Last seen: 11 years, 9 months
Re: Lion's mane wedge [Re: Anno]
    #2481018 - 03/29/04 08:06 PM (12 years, 8 months ago)


Thanks both for the how to!!

How much does the post office charge for
the mailing then??


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Invisiblesakura
Aussie Expat

Registered: 02/08/04
Posts: 592
Loc: Japan
Re: Lion's mane wedge [Re: charlieXxXxX]
    #2481370 - 03/29/04 09:28 PM (12 years, 8 months ago)

I just got a fruiting block of Japan's #1 shiitake strain. Gonna try Dr Wayne's 'cardboard agar' to make a few mailable cultures. Would love to trade with you.

The block is fruiting now, but they will take a week to grow out onto the medium. PM me if you're keen.


--------------------
Shrooms aren't everyone's cup of tea... (Some folks just eat 'em)


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Anonymous

Re: Lion's mane wedge [Re: sakura]
    #2483039 - 03/30/04 10:16 AM (12 years, 8 months ago)

I got a nice heavy producing lions mane for ya better than my old strain.


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OfflineJeRiKo1
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Registered: 02/25/03
Posts: 123
Loc: Germany
Last seen: 11 years, 7 months
Re: Lion's mane wedge [Re: charlieXxXxX]
    #2483202 - 03/30/04 11:43 AM (12 years, 8 months ago)

@Anno: Do you use any kind of sterilization on those ziplocks ? I think they are made of PE and can therefore not withstand high temperatures. I ordered some PP bags (in fact card-protectors for TCGs), they will be delivered this week. I will than make some test, how much heat for how long the will withstand. If anybody is interested in the results, I can post them.


--------------------
"All statements are true in some sense, false in some sense, meaningless in some sense, true and false in some sense, true and meaningless in some sense, false and meaningless in some sense, and true and false and meaningless in some sense.''


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Anonymous

Re: Lion's mane wedge [Re: JeRiKo1]
    #2483229 - 03/30/04 11:59 AM (12 years, 8 months ago)

I belive they are pretty sterile as is. its a plastic factory not to much organic stuff floating around in the air me thinks. I never had any problem with contams from plastic bags of this sort.


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OfflineJeRiKo1
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Registered: 02/25/03
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Re: Lion's mane wedge [Re: ]
    #2483237 - 03/30/04 12:03 PM (12 years, 8 months ago)

Good to know. And: If Anno is doing it, it cant be wrong :smile:
Will investigate anyways, for the real sterility freaks *bg*


--------------------
"All statements are true in some sense, false in some sense, meaningless in some sense, true and false in some sense, true and meaningless in some sense, false and meaningless in some sense, and true and false and meaningless in some sense.''


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InvisibleYidakiMan
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Re: Lion's mane wedge [Re: JeRiKo1]
    #2484224 - 03/30/04 05:52 PM (12 years, 8 months ago)

I put two wedges into mini centrifugal tubes and wrap them up. I get mini centrifugal tubes at a local science surplus store. 5 cents a piece.


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OfflineMycena
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Registered: 05/02/03
Posts: 270
Loc: indonesia
Last seen: 4 years, 7 months
Re: Lion's mane wedge [Re: charlieXxXxX]
    #2486129 - 03/31/04 03:34 AM (12 years, 8 months ago)

Same as Anno, though sometimes i just put it in something that bulky and still springy like thick folded paper or a brochure.

Lays flat and indistinguishable from a standard letter so cheap to send - maximum AU$1.65 anywhere in the world... now thats value

so far people whoce used thi smethod and sent to me ive had about 90% success rate, i only lost the golden oyster but this may have had hidden contams already. Zip lock bags are sterile, and you can assume any other fresh sealed plastic is too.

They do not autoclave well..:O


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OfflinecharlieXxXxX
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Registered: 03/29/04
Posts: 80
Loc: Earth, USA, WI, Madison
Last seen: 11 years, 9 months
Re: Lion's mane wedge [Re: Mycena]
    #2487362 - 03/31/04 04:45 PM (12 years, 8 months ago)


I tried your lay flat method with the half dozen cultures
I've sent out to those who PM'd me. I am hoping it works!

Umm, not to rain on your parade, but my worldwide mail fee
is USD$0.80!

"They do not autoclave well..:O"

lol


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OfflineMycena
mycoexplorer
Registered: 05/02/03
Posts: 270
Loc: indonesia
Last seen: 4 years, 7 months
Re: Lion's mane wedge [Re: charlieXxXxX]
    #2497982 - 04/01/04 08:58 AM (12 years, 8 months ago)

Well 6 months ago that was about US 0.80, with our dollar way now though we are getting ripped off :frown:

(However its a great time to buy books n stuff from the US cos our dollar goes further. Been spending all available reserves before bush gets kicked out and americas economy recovers :P

for example a US 90 dollar book would have cos me $160 -  6 months ago, now its dropped by approx $40 so its amazon fever in April :smile:)


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Offlineragadinks
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Re: Lion's mane wedge [Re: Mycena]
    #2500956 - 04/01/04 10:34 AM (12 years, 8 months ago)

So, Bush has really got his good sides as well :wink:


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InvisibleSpeeker

Registered: 02/11/04
Posts: 640
Re: Lion's mane wedge [Re: Mycena]
    #2520667 - 04/03/04 04:06 AM (12 years, 8 months ago)

How about using crown caps (bottle caps) as vessels for posting?  :tongue:



Wiped with alcohol they might be pretty clean...

Or how about making little agar plates from them?
(edit: 1-2 ml of agar would be adequate amount...)

Just some foil or tape (or both) to seal and
then tape the cap face down into a piece of cardboard...
(edit:I mean so that the agar is facing the cardboard.)

It would be quite durable package, I imagine..  :grin:



Edited by speeker (04/03/04 03:03 PM)


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OfflinecharlieXxXxX
Stranger

Registered: 03/29/04
Posts: 80
Loc: Earth, USA, WI, Madison
Last seen: 11 years, 9 months
Re: Lion's mane wedge [Re: Speeker]
    #2521349 - 04/03/04 01:28 PM (12 years, 8 months ago)


Seems like a good plan if one can keep the cap from
cutting through the envelope!

I would picture it more as a vessel to contain a sterile
package (i. e., a baggie) rather than a sterile vessel itself
but who knows?  :smile:


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InvisibleSpeeker

Registered: 02/11/04
Posts: 640
Re: Lion's mane wedge [Re: charlieXxXxX]
    #2523614 - 04/04/04 04:05 AM (12 years, 8 months ago)

(staged) pictures of a package that has been sent through the mail:


before


after

the cap didn't cut through the cardboard even when I
quite violently tried it - but I am not a postal worker, though..  :laugh:
(note the tape between the cap and the cardboard (vertical))


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OfflinecharlieXxXxX
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Registered: 03/29/04
Posts: 80
Loc: Earth, USA, WI, Madison
Last seen: 11 years, 9 months
Re: Lion's mane wedge [Re: sakura]
    #2649771 - 05/07/04 01:43 PM (12 years, 7 months ago)


I put the culture that you sent on H202 agar on 4-22.

Today the plate is colonized. It is the fastest shiitake culture

that I have by far. The only culture of any strain that is faster

is speekers' reishi.

Did you find me any Yamabiko Hon-shimeji yet?


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InvisibleSpeeker

Registered: 02/11/04
Posts: 640
Re: Lion's mane wedge [Re: charlieXxXxX]
    #2649984 - 05/07/04 02:51 PM (12 years, 7 months ago)

Quote:

charlieXxXxX said:

I put the culture that you sent on H202 agar on 4-22.
Today the plate is colonized.  It is the fastest shiitake culture
that I have by far.  The only culture of any strain that is faster
is speekers' reishi.
Did you find me any Yamabiko Hon-shimeji yet?




I think he has found that for you!  :smile:
http://www.shroomery.org/forums/showflat.php?Cat=&Number=2644508

That reishi sure is a fast colonizer, but I haven't been able to get
it to fruit yet..hope it will fruit...

About that bottle cap "hint" I made earlier in this thread..
It works, but you cannot say it's a very easy method...
Almost a 'chindogu' idea - needs developing...  :tongue:




:sun:

edit: some very promising looking growth is showing in the reishi
bags now..I had not inspected them for a while...


Edited by speeker (05/12/04 11:17 AM)


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Amazon Shop for: Agar, Autoclave, Petri Dish

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