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OfflineGooby
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Liquid Culture quality check
    #24789462 - 11/17/17 02:52 PM (11 months, 20 days ago)

Greetings! Im first time making LC. I would like to kindly ask you fellow shroomers to tell your opinion about this LC. I really cannot decide if my stuff looks legitt. So take a look and tell me please if this is ready to inject or trash... Thank you!







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InvisiblebodhisattaM
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Re: Liquid Culture quality check [Re: Gooby]
    #24789479 - 11/17/17 03:00 PM (11 months, 20 days ago)

Sorry it must get tested first. You cant tell shit by appearance


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Re: Liquid Culture quality check [Re: bodhisatta]
    #24789505 - 11/17/17 03:09 PM (11 months, 20 days ago)

so i cannot even know if this is myc or some fkin mold?


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InvisiblebodhisattaM
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Re: Liquid Culture quality check [Re: Gooby]
    #24789508 - 11/17/17 03:10 PM (11 months, 20 days ago)

Or it could be mold and mycelium. Hence the test

Put some on a petri dish and see if its clean or not


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InvisiblehamloafS
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Re: Liquid Culture quality check [Re: bodhisatta] * 1
    #24791353 - 11/18/17 12:49 PM (11 months, 19 days ago)

Quote:

bodhisatta said:
Sorry it must get tested first. You cant tell shit by appearance



This is not entirely true.  Visually, a LC can't be distinguished as clean, but you can easily tell if one is contamited by looking at it. 

https://www.shroomery.org/forums/showflat.php/Number/21068298#21068298


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InvisiblebodhisattaM
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Re: Liquid Culture quality check [Re: hamloaf]
    #24791366 - 11/18/17 12:57 PM (11 months, 19 days ago)

Yes if its obviously contaminated its obviously contaminated.. most of the time contaminated LC looks clean.


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Re: Liquid Culture quality check [Re: bodhisatta]
    #24793683 - 11/19/17 03:07 PM (11 months, 18 days ago)

He is growing really fast and the water starts to get cleaner. When i injected the spores it had light yellow color. I kinda trust him, tomorrow they will be injected.





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InvisiblebodhisattaM
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Re: Liquid Culture quality check [Re: Gooby]
    #24793685 - 11/19/17 03:08 PM (11 months, 18 days ago)

If you made your LC from spores you have a better chance of it being bad than good.

Quote:


Spore prints taken with care will be ready to use for cultivation or producing spore prints. Even grown in open air the amount of spores outweighs contaminants by a factor of millions to one. See this link for a better explanation.. As an example spore syringe might contain 10 contaminant spores and 100 million cubensis spores if made well. If the syringe is used to inoculate an agar petri dish or PF/BRF cake the contamination will be mitigated. As with the agar dish if a contaminant grows you can see it and transfer mycelium to a new agar dish and obtain a clean culture. In a PF cake the small amount of contamination usually will not cause a problem.

If you inoculate grain spawn or a liquid culture with a spore syringe the small amount of contamination can wreak havoc. With grain spawn the contaminant can lay dormant or unseen then become a problem when expanded. Grains get shaken and mixed with substrate which can amplify and spread a contaminant. The same concept applies with liquid culture but it's even more dangerous to use spores directly to inoculate a LC. As a single contaminant, regardless of how outnumbered it is by spores, can grow in the liquid broth and grow along with mushroom mycelium with no visual indication of contamination being present. Then it gets used to inoculate and infects everything down the line.





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Re: Liquid Culture quality check [Re: bodhisatta]
    #24793697 - 11/19/17 03:15 PM (11 months, 18 days ago)

I will put them in PFs. Later i will try your advice when i start the monutub thing.


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InvisiblebodhisattaM
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Re: Liquid Culture quality check [Re: Gooby]
    #24793711 - 11/19/17 03:23 PM (11 months, 18 days ago)

Best of luck. But i would if i were you test that LC on a few cakes  or a jar or two of spawn before using it on a bunch of stuff.



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My Q&A thread. Ask me questions here rather than in Private Message


First rule of mushroom cultivation. Get high on your own supply.


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OfflineGooby
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Re: Liquid Culture quality check [Re: bodhisatta]
    #24793809 - 11/19/17 04:49 PM (11 months, 18 days ago)

thanks bro. Thats exactly what i will do :smile:)


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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