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gourmet
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Registered: 10/17/17
Posts: 75
Last seen: 6 years, 2 months
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Re: No pour mini rounds and big lots containers pooling water leading to bacteria growth. [Re: Josex]
#24793599 - 11/19/17 12:16 PM (7 years, 1 month ago) |
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Are those all glass plates?
How much do you pay per plate?
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,999
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Re: No pour mini rounds and big lots containers pooling water leading to bacteria growth. [Re: gourmet]
#24793618 - 11/19/17 12:28 PM (7 years, 1 month ago) |
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A box of 500 for cheap. They're pp5 plastic.
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gourmet
Stranger
Registered: 10/17/17
Posts: 75
Last seen: 6 years, 2 months
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Re: No pour mini rounds and big lots containers pooling water leading to bacteria growth. [Re: Josex]
#24793892 - 11/19/17 03:42 PM (7 years, 1 month ago) |
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Got a link where I could buy some?
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JHOVA
Post whore



Registered: 02/17/17
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Re: No pour mini rounds and big lots containers pooling water leading to bacteria growth. [Re: gourmet]
#24793917 - 11/19/17 03:57 PM (7 years, 1 month ago) |
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He linked it in blue^^^
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JFlint

Registered: 11/12/17
Posts: 115
Last seen: 6 years, 7 months
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Re: No pour mini rounds and big lots containers pooling water leading to bacteria growth. [Re: Munchauzen]
#24817791 - 11/30/17 08:15 PM (7 years, 1 month ago) |
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Quote:
Munchauzen said:
Quote:
Jonhova said: The mini rounds got covered in foil.
I took em out of the PC this morning and flicked the sides so the condensate fell down and removed the foil so it can breathe.
I recommend that you always do any agar upside down. Sit them upside down in your SAB, open them upside down, and tilt them sideways to take transfers out.
Doing so will completely eliminate any concerns you have with water because it simply becomes a non issue
How exactly does working with it upside down eliminate the issue with water? are you simply pouring off the water?
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JHOVA
Post whore



Registered: 02/17/17
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Re: No pour mini rounds and big lots containers pooling water leading to bacteria growth. [Re: JFlint]
#24817884 - 11/30/17 08:59 PM (7 years, 1 month ago) |
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Quote:
JFlint said:
Quote:
Munchauzen said:
Quote:
Jonhova said: The mini rounds got covered in foil.
I took em out of the PC this morning and flicked the sides so the condensate fell down and removed the foil so it can breathe.
I recommend that you always do any agar upside down. Sit them upside down in your SAB, open them upside down, and tilt them sideways to take transfers out.
Doing so will completely eliminate any concerns you have with water because it simply becomes a non issue
How exactly does working with it upside down eliminate the issue with water? are you simply pouring off the water?
It doesnt. Upside down is best practice for avoiding shit getting inside your pasties.
If you look up a couple replies i figured out what was causing the pooling and extra condensation.
It was due to leaving all my media in the PC overnight. Josex had an idea of removing the pasties as soon as the pressure reached zero. It worked like a charm.
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Munchauzen


Registered: 06/22/11
Posts: 14,348
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Re: No pour mini rounds and big lots containers pooling water leading to bacteria growth. [Re: JFlint]
#24817919 - 11/30/17 09:23 PM (7 years, 1 month ago) |
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Quote:
JFlint said:
Quote:
Munchauzen said:
Quote:
Jonhova said: The mini rounds got covered in foil.
I took em out of the PC this morning and flicked the sides so the condensate fell down and removed the foil so it can breathe.
I recommend that you always do any agar upside down. Sit them upside down in your SAB, open them upside down, and tilt them sideways to take transfers out.
Doing so will completely eliminate any concerns you have with water because it simply becomes a non issue
How exactly does working with it upside down eliminate the issue with water? are you simply pouring off the water?
Gravity. Think about it. If you have a cup full of water and turn it upside-down, what happens? Besides, I've said it a million times, condensation isn't something you even have to worry about. Leave it or not, it doesn't make a difference. Condensation doesn't cause bacterial growth. I've left hundreds of non-inoculated plates sit for weeks and even months, and they do not contaminate regardless of the condensation they all have. Even the example josex gave involed introducing alcohol and a wet filter and not just simply condensation.
So you can either just stop worrying about it, or dump the water out. Thems your best options.
Edited by Munchauzen (11/30/17 09:37 PM)
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JHOVA
Post whore



Registered: 02/17/17
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Re: No pour mini rounds and big lots containers pooling water leading to bacteria growth. [Re: Munchauzen]
#24818153 - 12/01/17 01:10 AM (7 years, 1 month ago) |
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obstructive condensation is not a problem. Pooling is as my pics show.
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JFlint

Registered: 11/12/17
Posts: 115
Last seen: 6 years, 7 months
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Re: No pour mini rounds and big lots containers pooling water leading to bacteria growth. [Re: Munchauzen]
#24818334 - 12/01/17 06:07 AM (7 years, 1 month ago) |
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Quote:
Munchauzen said:
Quote:
JFlint said:
Quote:
Munchauzen said:
Quote:
Jonhova said: The mini rounds got covered in foil.
I took em out of the PC this morning and flicked the sides so the condensate fell down and removed the foil so it can breathe.
I recommend that you always do any agar upside down. Sit them upside down in your SAB, open them upside down, and tilt them sideways to take transfers out.
Doing so will completely eliminate any concerns you have with water because it simply becomes a non issue
How exactly does working with it upside down eliminate the issue with water? are you simply pouring off the water?
Gravity. Think about it. If you have a cup full of water and turn it upside-down, what happens? Besides, I've said it a million times, condensation isn't something you even have to worry about. Leave it or not, it doesn't make a difference. Condensation doesn't cause bacterial growth. I've left hundreds of non-inoculated plates sit for weeks and even months, and they do not contaminate regardless of the condensation they all have. Even the example josex gave involed introducing alcohol and a wet filter and not just simply condensation.
So you can either just stop worrying about it, or dump the water out. Thems your best options.
Munch, I agree with you that the condensation wouldnβt cause contam by itself, itβs pooking I am more concerned with, but less so with contam. Iβm concerned with the mycelium not colonizing the agar with a pool of water on the surface. I am still confused a bit with your statement. By doing things upside down, youβre simply suggesting to let the excess water drain off the surface of the agar and onto the lid/out of the plate entirely? What is YOUR main concern as to why you would do things upside down? Sorry if Iβm beating a dead horse here, I just like to have a thorough understanding.
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nubgrower
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Registered: 08/27/17
Posts: 274
Loc: Moon 13 the Moon.
Last seen: 7 years, 1 month
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Re: No pour mini rounds and big lots containers pooling water leading to bacteria growth. [Re: JFlint]
#24818441 - 12/01/17 07:59 AM (7 years, 1 month ago) |
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there are two benefits of keeping them upside down: 1 water leaves the surface of the agar, and 2 no contams will fall onto the agar when you open it.
The most you will ever turn the plate away from upside down is sideways to get your inoc loop in there or scalpel for removing a wedge...
-------------------- Great teks here.
And here.
Fortunately, Iβm adhering to a pretty strict, uh, drug regimen to keep my mind, you know, uh, limber
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JFlint

Registered: 11/12/17
Posts: 115
Last seen: 6 years, 7 months
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Re: No pour mini rounds and big lots containers pooling water leading to bacteria growth. [Re: nubgrower]
#24818462 - 12/01/17 08:16 AM (7 years, 1 month ago) |
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Except for maybe transferring a mycelium sample to a clean plate? It would fall to the edge of the plate if performing a transfer with the plate sideways...
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catnip40
xΰΈ¬ΰΉ Sect.β΄CubensaeΜ· .Μ΅



Registered: 03/09/12
Posts: 1,031
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Re: No pour mini rounds and big lots containers pooling water leading to bacteria growth. [Re: JFlint]
#24818488 - 12/01/17 08:36 AM (7 years, 1 month ago) |
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JHOVA
Post whore



Registered: 02/17/17
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Re: No pour mini rounds and big lots containers pooling water leading to bacteria growth. [Re: JFlint]
#24818510 - 12/01/17 08:52 AM (7 years, 1 month ago) |
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Munchs agar videos clearly demonstrste how to pasty plate side ways. Check In his signature.
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nubgrower
Dude



Registered: 08/27/17
Posts: 274
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Re: No pour mini rounds and big lots containers pooling water leading to bacteria growth. [Re: JFlint]
#24819902 - 12/01/17 07:23 PM (7 years, 1 month ago) |
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Quote:
JFlint said: Except for maybe transferring a mycelium sample to a clean plate? It would fall to the edge of the plate if performing a transfer with the plate sideways...
Well, from experience and the experience of countless others, you are wrong. It is quite easy to put a sample on a new plate sideways and have it stick.
-------------------- Great teks here.
And here.
Fortunately, Iβm adhering to a pretty strict, uh, drug regimen to keep my mind, you know, uh, limber
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JFlint

Registered: 11/12/17
Posts: 115
Last seen: 6 years, 7 months
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Re: No pour mini rounds and big lots containers pooling water leading to bacteria growth. [Re: nubgrower]
#24820177 - 12/01/17 09:42 PM (7 years, 1 month ago) |
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It was only a theory. If it is not the case I surely donβt mean to argue.
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ghaleon
Stranger
Registered: 10/12/16
Posts: 60
Last seen: 6 years, 5 months
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Re: No pour mini rounds and big lots containers pooling water leading to bacteria growth. [Re: JHOVA]
#24888182 - 01/03/18 07:08 PM (7 years, 14 days ago) |
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Quote:
JHOVA said: Thank you Josex for solving my problem.
My problem was water somehow sneaking inside under the paper towel through SFDS and MP tape during or after the PC cycle.
What he suggested was removing all media containers once the PC's pressure reaches zero.
Here are my results

No pooling no bacteria. Slight moisture on the walls i can live with. Also lf note is that the paper towels are not damp or stuck to the MP tape.
Anyone having pooling issues try removing your no pours from the PC.
I always remove my pasty plates when the pressure reaches zero and still get condensation on the surface of the agar. I use foil but no paper towel.
I do agar work exactly as outlined in Munch's videos and still get bacterial contamination. I think my problem might be what the other posted suggests in that perhaps some of the rubbing alcohol is getting into my plates after I wipe the lid. I've considered wiping the lid with a dry paper towel instead but haven't tried that yet.
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flyhighfunguy

Registered: 09/13/17
Posts: 1,553
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Re: No pour mini rounds and big lots containers pooling water leading to bacteria growth. [Re: Munchauzen]
#24888259 - 01/03/18 07:41 PM (7 years, 14 days ago) |
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Quote:
Munchauzen said:
Quote:
Jonhova said: The mini rounds got covered in foil.
I took em out of the PC this morning and flicked the sides so the condensate fell down and removed the foil so it can breathe.
I recommend that you always do any agar upside down. Sit them upside down in your SAB, open them upside down, and tilt them sideways to take transfers out.
Doing so will completely eliminate any concerns you have with water because it simply becomes a non issue
Do you let them colonize upside down as well, or you only have them upside down when doing transfers and stuff?
-------------------- If you need any help achieving proper field capacity, please check out this video. I hope it helps you guys out!
https://www.youtube.com/watch?v=HskufFlXEo4
https://www.shroomery.org/forums/showflat.php/Number/25087128/page/1
Edited by flyhighfunguy (01/03/18 07:42 PM)
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ghaleon
Stranger
Registered: 10/12/16
Posts: 60
Last seen: 6 years, 5 months
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Re: No pour mini rounds and big lots containers pooling water leading to bacteria growth. [Re: flyhighfunguy]
#24888287 - 01/03/18 07:58 PM (7 years, 14 days ago) |
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Quote:
flyhighfunguy said:Do you let them colonize upside down as well, or you only have them upside down when doing transfers and stuff?
I do not believe you're supposed to let them colonize upside down:
Quote:
PussyFart said:
Quote:
RogerRabbit said: No. You should not store petri dishes upside down. It forces the water in the agar to 'drain' out, collecting on the lid, which then floods the culture when you right the dish/jar. In addition, mycelium grows best on the top(away from gravity) surface of a flat plane, and when forced to grow upside down, growth and performance is slower. Since the whole point of isolation on agar is to encourage the fastest growth possible, it should be done in actual Petri dishes that are stored right-side-up for best results. RR
http://www.shroomery.org/forums/showflat.php/Number/11513433#11513433
Quote:
RogerRabbit said: NEVER turn petri dishes upside down. Bacteria labs often do this because they're only growing a culture for a few days to a week. We use our petri dishes much longer than that and gravity will pull the moisture out of the agar, creating a 'lake' in the lid if inverted. RR
http://www.shroomery.org/forums/showflat.php/Number/16096716#16096716
Quote:
RogerRabbit said: Never store dishes upside down. RR
http://www.shroomery.org/forums/showflat.php/Number/17588971#17588971
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JHOVA
Post whore



Registered: 02/17/17
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Re: No pour mini rounds and big lots containers pooling water leading to bacteria growth. [Re: ghaleon]
#24888312 - 01/03/18 08:07 PM (7 years, 14 days ago) |
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They colonize on their sides and right side up in shoeboxes . Sometimes upside down. In a SAB everything is upside down or at an angle.
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