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InvisibleJHOVA
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No pour mini rounds and big lots containers pooling water leading to bacteria growth.
    #24785959 - 11/15/17 09:25 PM (7 years, 2 months ago)

The problem

Quote:






This agar is over a week old and still holding onto quite a bit of water. I drilled holes out of the big lots containers and slapped
Some SFD's on them thinking extra ventilation would help dry them out. Its worked before.


Preparation
The agar recipe that worked for me before was 2 table spoons brick agar(?) from the asian store put in a coffee grinder and 2 teaspoons potato flakes, no karo.  Stiff and easy to transfer.

Quote:


6 grams brf
9 grams agar
400 ml water




My agar prep is weigh out everything on the scale, add it to a pot.
Let it simmer for a second. Remove from heat. Let it cool down in the pot.


Before it starts solidifying spoon 1.5 tablespoons in each container.
Let it sit for 25 minutes until the agar is solidified and there is no visible
condensation on the sides of the no pour plates.

Vent for 15 minutes.

I used to get condensation for a day or 2 thrn the agar would wick all the excess moisture.
Now its not doing that at all.

I cover the minirounds with foil. I dont on the big lots co tainers.

The last round i brought the temperature down from 3 to 2 after the 45 minute pc cycle for 10 minutes
then to 1 for 10 minutes and then finally off.

This is what ive been using.
Boogie was kind enough to link me to this killer agar deal on amazon for 200 grams.




Going forward
I think i should make some plates on the old pasty recipe as a test. Maybe my agar or brf are to blame?:shrug:

I noticed black pin mold on one plates i never opened on the big lots containers.
Im thinking the seal was compromised. Never seen mold on a unopened plate before.

What do you guys think?


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InvisibleLemurLemur
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Re: No pour mini rounds and big lots containers pooling water leading to bacteria growth. [Re: JHOVA]
    #24786175 - 11/16/17 01:26 AM (7 years, 2 months ago)

Let it cool unlevel. Tap an pour out in sab.


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InvisibleJHOVA
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Re: No pour mini rounds and big lots containers pooling water leading to bacteria growth. [Re: LemurLemur]
    #24786337 - 11/16/17 05:11 AM (7 years, 2 months ago)

Will do. I made a batch with the "new" agar powder and the old potato flakes at
Quote:


10 grams flakes ,
7 agar,
450 ml water.




I found that recipe here


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InvisibleZiran
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Re: No pour mini rounds and big lots containers pooling water leading to bacteria growth. [Re: JHOVA]
    #24786395 - 11/16/17 06:13 AM (7 years, 2 months ago)

did you foil em before you pced? that happened to me when I didnt foil my plates.


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InvisibleJHOVA
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Re: No pour mini rounds and big lots containers pooling water leading to bacteria growth. [Re: Ziran]
    #24786534 - 11/16/17 07:53 AM (7 years, 2 months ago)

The mini rounds got covered in foil.

I took em out of the PC this morning and flicked the sides so the
condensate fell down and removed the foil so it can breathe.


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InvisibleMunchauzen
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Re: No pour mini rounds and big lots containers pooling water leading to bacteria growth. [Re: JHOVA] * 2
    #24787849 - 11/16/17 06:44 PM (7 years, 2 months ago)

Quote:

Jonhova said:
The mini rounds got covered in foil.

I took em out of the PC this morning and flicked the sides so the
condensate fell down and removed the foil so it can breathe.



I recommend that you always do any agar upside down. Sit them upside down in your SAB, open them upside down, and tilt them sideways to take transfers out.

Doing so will completely eliminate any concerns you have with water because it simply becomes a non issue

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Offline99.99
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Re: No pour mini rounds and big lots containers pooling water leading to bacteria growth. [Re: Munchauzen]
    #24787922 - 11/16/17 07:09 PM (7 years, 2 months ago)

Cover biglot containers with foil ore water drips through microportape
After pc but before use flick the lids with your finger to disperce water off lids
The small amount left with either reasorb after inoculation ore you can poor it off whi

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InvisibleJosex
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Re: No pour mini rounds and big lots containers pooling water leading to bacteria growth. [Re: Munchauzen] * 2
    #24788001 - 11/16/17 07:40 PM (7 years, 2 months ago)

Quote:

Munchauzen said:
I recommend that you always do any agar upside down. Sit them upside down in your SAB, open them upside down, and tilt them sideways to take transfers out.

Doing so will completely eliminate any concerns you have with water because it simply becomes a non issue



:whathesaid:
This is not just a 'different' way of working with no-pours, this is the WAY and everybody should be doing this, seriously.
Up until recently I was having a huge mold problem. A neighbour dropped a wet towel that was sitting on a puddle in my backyard and I just didn't notice. The thing got entirely covered in green and was contaminating the whole house like you wouldn't believe. My SAB work became a nightmare, and I had to try different ways of moving in the SAB to see what gave me the less contamination. Small tweaks in the way I moved or did things made a hell of a difference.
I've always worked with the plates upside-down but I decided to try working with them upright just to see if that could give less moldy plates. It gave me double...


I don't want anybody to take this personally but I feel I'm going to explode if I don't say it...
  • Drilling big holes so the water in the plates evaporates quicker and using just 1 layer of mp tape: bullshit and asking for trouble. Drill tiny holes and use at least 2 layers, condensation is not an issue.
  • Letting the agar cool in the pot: bullshit, pour it hot.
  • Waiting for the condensation in the plates to go after pouring: bullshit, it won't get you less condensation after the cycle.
  • Wrapping in foil: Bullshit, it won't give you less condensation and there is a simple way to get away without using foil or paper towels and the tape will come out dry after the cycle.
  • Bringing the pc up to pressure very slowly: Bullshit, turn the burner all the way up.
  • Bringing the temp down slowly: Bullshit, the gas should be set low anyway after bringing the pc up to pressure.
  • Keeping the plates in the pc overnight: Bullshit and horrible. Take them out still hot so the agar sets level on a table.
  • Finger flicking the plates to get the condensation out: Horrible, all the water will pool on the bottom.
  • Wiping the walls of the container with a paper towel in the SAB: :facepalm:

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InvisibleMunchauzen
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Re: No pour mini rounds and big lots containers pooling water leading to bacteria growth. [Re: Josex]
    #24788018 - 11/16/17 07:46 PM (7 years, 2 months ago)

ya for reals I've seen most of the suggestions and they are indeed :facepalm:

just use the damn things upside down and move on with your life. no need to waste time on it.

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InvisibleJHOVA
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Re: No pour mini rounds and big lots containers pooling water leading to bacteria growth. [Re: Josex]
    #24788078 - 11/16/17 08:11 PM (7 years, 2 months ago)

hmm well i have switched agar recipes since i got powdered agar and have been having a hell of a time.

i tried the 1-2-3 recipe, then brf agar, now back to pda with flakes. always a bunch of condensation that turns into the first pics of bacteria.

Some of the mini rounds were soaked. most damp even with foil and paper towels. I was cheaping out on thin paper towels so i replaced those for thicker name brand. double and triple fresh MP tape.

Quote:

Munchauzen said:
Quote:

Jonhova said:
The mini rounds got covered in foil.

I took em out of the PC this morning and flicked the sides so the
condensate fell down and removed the foil so it can breathe.



I recommend that you always do any agar upside down. Sit them upside down in your SAB, open them upside down, and tilt them sideways to take transfers out.

Doing so will completely eliminate any concerns you have with water because it simply becomes a non issue




if theres considerable water content inside after a pc cycle are you saying i should let the water pool on the MP or SFD upside down post PC cycle? doesnt that compromise the filter?

i never cared about water content before the last 3 cycles. the water would evaporate now its just sitting there never evaporating. i dont understand what has changed beyond the agar recipe that would make such a bid difference.

I dont use all my agar right away sometimes a week or two before doing transfers. the pics i posted in the op are from a week ago.

that looks like bacteria to me. and theres a lot of water so putting 2 and 2 together i think its safe to say either the filter is compromised or the water content inside is helping the bacteria proliferate.

does it matter if i pack my PC super full as far as water getting inside of my pasty plates? ive done maybe a dozen rounds with these or more.
Quote:

Josex said:

Quote:

Munchauzen said:
I recommend that you always do any agar upside down. Sit them upside down in your SAB, open them upside down, and tilt them sideways to take transfers out.

Doing so will completely eliminate any concerns you have with water because it simply becomes a non issue



:whathesaid:
This is not just a 'different' way of working with no-pours, this is the WAY and everybody should be doing this, seriously.
Up until recently I was having a huge mold problem. A neighbour dropped a wet towel that was sitting on a puddle in my backyard and I just didn't notice. The thing got entirely covered in green and was contaminating the whole house like you wouldn't believe. My SAB work became a nightmare, and I had to try different ways of moving in the SAB to see what gave me the less contamination. Small tweaks in the way I moved or did things made a hell of a difference.
I've always worked with the plates upside-down but I decided to try working with them upright just to see if that could give less moldy plates. It gave me double...


I don't want anybody to take this personally but I feel I'm going to explode if I don't say it...
  • Drilling big holes so the water in the plates evaporates quicker and using just 1 layer of mp tape: bullshit and asking for trouble. Drill tiny holes and use at least 2 layers, condensation is not an issue.
  • Letting the agar cool in the pot: bullshit, pour it hot.
  • Waiting for the condensation in the plates to go after pouring: bullshit, it won't get you less condensation after the cycle.
  • Wrapping in foil: Bullshit, it won't give you less condensation and there is a simple way to get away without using foil or paper towels and the tape will come out dry after the cycle.
  • Bringing the pc up to pressure very slowly: Bullshit, turn the burner all the way up.
  • Bringing the temp down slowly: Bullshit, the gas should be set low anyway after bringing the pc up to pressure.
  • Keeping the plates in the pc overnight: Bullshit and horrible. Take them out still hot so the agar sets level on a table.
  • Finger flicking the plates to get the condensation out: Horrible, all the water will pool on the bottom.
  • Wiping the walls of the container with a paper towel in the SAB: :facepalm:





everything you mention all sounds like a lot of bs i agree. but when you agar is coming out fucked up and haggard you look for things you can improve desperately.


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InvisibleJosex
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Re: No pour mini rounds and big lots containers pooling water leading to bacteria growth. [Re: JHOVA]
    #24788104 - 11/16/17 08:24 PM (7 years, 2 months ago)

Jon, there are some instances in which condensation can give you bacteria.
Maybe you get your plates almost soaking wet with alcohol when you sanitize them in the SAB and open the lid with the plate upright. The alcohol can get in contact with some of the water, the water might drip down to the bottom and that will give you bacteria. It happened to me before.

Don't overthink stuff and quit using SFD for your plates. In this hobby, if it isn't simple it ain't good, if you're making it complicated you sure as hell are doing something wrong. When in doubt, picture yourself as the laziest mofo out there that don't give a fuck, how would you do things then? Exactly, that's the way.

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InvisibleJHOVA
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Re: No pour mini rounds and big lots containers pooling water leading to bacteria growth. [Re: Josex]
    #24788120 - 11/16/17 08:33 PM (7 years, 2 months ago)

I dunno man i watched munchs videos and copied those to the best of my ability. I do everything upside down in the sab.

I have everything elevated on a cookie rack.

I put SFD on the biglots clear containers when they had obvious signs of bacteria while being 2 weeks old and considerable water inside.

Lol i want to be lazy instead i have to be inspector gadget figuring out why my agar is sucking dick.


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InvisibleJosex
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Re: No pour mini rounds and big lots containers pooling water leading to bacteria growth. [Re: JHOVA]
    #24788142 - 11/16/17 08:44 PM (7 years, 2 months ago)

Quote:

I noticed black pin mold on one plates i never opened on the big lots containers.
Im thinking the seal was compromised. Never seen mold on a unopened plate before.



Any plastic container with snap-on lid will get you some moldy plates without opening them if the spore load in your house is seriously bad, but I think in your case is probably the lid, maybe those containers aren't good for this.
If you drill a tiny hole and cover it with 3 layers of mp tape, there's no way anything could be getting in the containers through the filter, even if you live in a moldy ass house.

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Offline99.99
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Re: No pour mini rounds and big lots containers pooling water leading to bacteria growth. [Re: JHOVA]
    #24788174 - 11/16/17 08:56 PM (7 years, 2 months ago)

Quote:

Jonhova said:
I dunno man i watched munchs videos and copied those to the best of my ability. I do everything upside down in the sab.

I have everything elevated on a cookie rack.

I put SFD on the biglots clear containers when they had obvious signs of bacteria while being 2 weeks old and considerable water inside.

Lol i want to be lazy instead i have to be inspector gadget figuring out why my agar is sucking dick.




Put your stuff in ziplocks right after your sab ore flowhood
Keep some cleaner when you want to use them again to

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Re: No pour mini rounds and big lots containers pooling water leading to bacteria growth. [Re: 99.99]
    #24788286 - 11/16/17 09:54 PM (7 years, 2 months ago)

Should agar have a film on it? Am i being anal?

Made this last night

10 grams agar
7 potato flakes
Drop of karo
450 ml




Nasty shit



Thats only the first 3 plates i opened up. If its like the last couple batches then every plate is fucked.


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Re: No pour mini rounds and big lots containers pooling water leading to bacteria growth. [Re: JHOVA]
    #24788402 - 11/16/17 11:30 PM (7 years, 2 months ago)

Are you venting your PC for the full ten minutes ??

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Re: No pour mini rounds and big lots containers pooling water leading to bacteria growth. [Re: 99.99]
    #24788416 - 11/16/17 11:43 PM (7 years, 1 month ago)

15 minutes


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Re: No pour mini rounds and big lots containers pooling water leading to bacteria growth. [Re: JHOVA]
    #24793418 - 11/19/17 10:41 AM (7 years, 1 month ago)

Thank you Josex for solving my problem.

My problem was water somehow sneaking inside under the paper towel through SFDS and MP tape during or after the PC cycle.

What he suggested was removing all media containers once the PC's pressure reaches zero.

Here are my results




No pooling no bacteria. Slight moisture on the walls i can live with.
Also lf note is that the paper towels are not damp or stuck to the MP tape.

Anyone having pooling issues try removing your no pours from the PC.:cheers:


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Re: No pour mini rounds and big lots containers pooling water leading to bacteria growth. [Re: JHOVA]
    #24793474 - 11/19/17 11:13 AM (7 years, 1 month ago)

:soproud:
Awesome dude! you owe me two now (jk) :douchewink:

This is a batch of agar (120 plates) still hot out of the pc:

No paper towels or foil as you can see and the tape comes out dry if you do a little trick.
I wait 15 min or so till the agar sets and then put them in a plastic tote.

If you do a tiny hole through the side and cover it with 3 layers of mp tape these fuckers can last you fresh a long ass time. Longest I've kept them unused is 40 days and they looked like fresh out of the pc. But you can even keep them for longer no problem, so whether you do lots of agar or not, prepping plates beast mode this way is very convenient, as they keep fresh for a long time so you always have blank plates handy.

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Re: No pour mini rounds and big lots containers pooling water leading to bacteria growth. [Re: Josex]
    #24793490 - 11/19/17 11:18 AM (7 years, 1 month ago)

:vibin:  :vibin:  :vibin:  :vibin:


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