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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 11 months, 16 days
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Re: Updated Bottle Tek New and improved [Re: hamloaf]
#24740943 - 10/27/17 10:18 AM (6 years, 6 months ago) |
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Thanks ham I'm on it
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 11 months, 16 days
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Re: Updated Bottle Tek New and improved [Re: tombosley8]
#24742125 - 10/27/17 08:06 PM (6 years, 6 months ago) |
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ok I made a huge batch and tried to somehow calculate making enough to fill my sterilizers and trying to figure out how much dry grain per brick of coir to make a 1:1 ratio mixture once hydrated.
I will see how well I did in a little bit.
Is it best to do 1:1 grain and coir ratio? or less grain?
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Edited by tombosley8 (10/27/17 10:02 PM)
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Updated Bottle Tek New and improved [Re: hamloaf]
#24746348 - 10/29/17 08:07 PM (6 years, 6 months ago) |
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Quote:
hamloaf said:
Quote:
tombosley8 said: Going to be trying the just oat and coir recipe tommorrow and I am not sure how much water to use or the best way to prep it.
Would you hydrate the grain and the coir separately or would you pour the pot of boiling grain into the coir like how the op recipe describes?
AND is there an easy way to figure out how much water is needed for said amount of grain to boil/hydrate and just add an extra gallon for hydrating the coir?
Any suggestion/info on the subject would be greatly appreciated so I know I'm going about this right.
Just go about your normal grain, and coir prep technique, mix them together, and place them in the bags once completed, then sterilize.
This but I'd still use the water from the grain prep to hydrate your coir.
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hamloaf
Pork Block ®©™√



Registered: 12/23/09
Posts: 21,754
Loc: ation undisclosed.
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Re: Updated Bottle Tek New and improved [Re: MudaFuka]
#24746360 - 10/29/17 08:09 PM (6 years, 6 months ago) |
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Hi muda!
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 11 months, 16 days
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Re: Updated Bottle Tek New and improved [Re: MudaFuka]
#24746389 - 10/29/17 08:23 PM (6 years, 6 months ago) |
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Quote:
MudaFuka said:
Quote:
hamloaf said:
Quote:
tombosley8 said: Going to be trying the just oat and coir recipe tommorrow and I am not sure how much water to use or the best way to prep it.
Would you hydrate the grain and the coir separately or would you pour the pot of boiling grain into the coir like how the op recipe describes?
AND is there an easy way to figure out how much water is needed for said amount of grain to boil/hydrate and just add an extra gallon for hydrating the coir?
Any suggestion/info on the subject would be greatly appreciated so I know I'm going about this right.
Just go about your normal grain, and coir prep technique, mix them together, and place them in the bags once completed, then sterilize.
This but I'd still use the water from the grain prep to hydrate your coir.
Thank you!
next Time....
this time I went with just straight bucket tek'd coir and oats steeped for 30 min and drained and rinsed with cold.
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dream.culture
Stranger


Registered: 08/08/17
Posts: 115
Last seen: 4 months, 25 days
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Re: Updated Bottle Tek New and improved [Re: tombosley8]
#24753802 - 11/02/17 07:49 AM (6 years, 6 months ago) |
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Trying grain and coir(wheat berries 1:1). Just inoculated these jars. Excited to see the results.

we all know mushrooms will tell you themselves how to grow them only if we are listening and I just realized that the best way to learn how to listen to your mushrooms is to eat them.
They just told me that Muda is right coir and grain is the way to go. 
Edited by dream.culture (11/02/17 07:52 AM)
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Spindlymass
wafer thin.



Registered: 09/25/17
Posts: 957
Loc:
Last seen: 1 month, 12 days
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Re: Updated Bottle Tek New and improved [Re: dream.culture]
#24766913 - 11/07/17 03:59 PM (6 years, 6 months ago) |
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In case it helps anyone out, I've managed a work around if you have a small 6 qt. pressure cooker.
Mine only fits one ziploc quart jar in the middle. Needless to say, that's a lot of pressure cooking for a 10 jar batch.
Now, there is a very good chance I have my head up my ass on this one, and please feel free to tell me.
I can fit 2, 1.75 qt. (1.7L) pyrex bowls in my little PC . The equivalent of 3.5 quart jars.

I've used a large canning pot of boiling water for 1 hour to (hopefully) sterilize the empty jars and lids. I'll take both the jars and PC'd pyrexes to my SAB and load them.
Sure, it introduces an extra step for contamination and it strays from the ease of the original tek but allows me to use the big boy jars...
-------------------- enough knowledge to get into trouble. Not nearly enough to get out again.
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Munchauzen


Registered: 06/22/11
Posts: 14,346
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Re: Updated Bottle Tek New and improved [Re: Spindlymass]
#24767433 - 11/07/17 07:15 PM (6 years, 6 months ago) |
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is there a lid under the foil?
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Spindlymass
wafer thin.



Registered: 09/25/17
Posts: 957
Loc:
Last seen: 1 month, 12 days
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Re: Updated Bottle Tek New and improved [Re: Munchauzen]
#24767592 - 11/07/17 08:09 PM (6 years, 6 months ago) |
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No. Hahaha!
For 2 reasons :
1. The lids are LDPE 4 and won't withstand the 2 hour pressure cooking
2. The pyrex bowls won't fit one on top of the other in my little PC with conventional lids. That's why in the photo you can see one bowl's tinfoil is stepped in. It's a tight fit.
I've taken a batch of 2 out already and have had no issues with the water getting in.
... Not ideal, I know...
-------------------- enough knowledge to get into trouble. Not nearly enough to get out again.
Edited by Spindlymass (11/09/17 11:26 PM)
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Knockoutartist
Night Night


Registered: 08/09/17
Posts: 66
Last seen: 3 years, 2 months
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Re: Updated Bottle Tek New and improved [Re: Spindlymass]
#24783469 - 11/14/17 08:25 PM (6 years, 6 months ago) |
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Quick question from a noob:
Everything is going along nicely, ziploc quart jars unscrwed 3/4", definitely nice white rhizomorphic mycelium growth, but it's been about a 4-5 weeks already and most of them are only 60% colonized. Is this normal? I started off with a MS print and have transferred several times on agar so I'm sure everything is clean, but I'm just wondering what's taking so long.
Temps at night are around 66, during the day around 72. I'm guessing it's the colder night time temps halting things? Be more patient?
Thanks in advance.
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Nobler Hino
a dojo and a forge?!


Registered: 08/29/15
Posts: 1,780
Loc: Deep Ellum
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its not the temps, did you use a liquid culture or wedge?
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   "The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me.” – Maria Sabina
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Knockoutartist
Night Night


Registered: 08/09/17
Posts: 66
Last seen: 3 years, 2 months
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Re: Updated Bottle Tek New and improved [Re: Nobler Hino]
#24784040 - 11/15/17 05:30 AM (6 years, 5 months ago) |
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Quote:
Nobler Hino said: its not the temps, did you use a liquid culture or wedge?
LI for most of them, a tiger drop for three.
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JHOVA
Post whore



Registered: 02/17/17
Posts: 4,727
Loc:
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Pics help
-------------------- 🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
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Munchauzen


Registered: 06/22/11
Posts: 14,346
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Quote:
Knockoutartist said: Quick question from a noob:
Everything is going along nicely, ziploc quart jars unscrwed 3/4", definitely nice white rhizomorphic mycelium growth, but it's been about a 4-5 weeks already and most of them are only 60% colonized. Is this normal? I started off with a MS print and have transferred several times on agar so I'm sure everything is clean, but I'm just wondering what's taking so long.
Temps at night are around 66, during the day around 72. I'm guessing it's the colder night time temps halting things? Be more patient?
Thanks in advance.
did you shake them yet? if not, shake them.
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,998
Loc:
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Re: Updated Bottle Tek New and improved [Re: Spindlymass]
#24784138 - 11/15/17 06:44 AM (6 years, 5 months ago) |
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Quote:
Munchauzen said: is there a lid under the foil?
Quote:
Spindlymass said: No. Hahaha!
For 2 reasons :
1. The lids are LDPE 4 and won't withstand the 2 hour pressure cooking
2. The pyrex bowls won't fit one on top of the other in my little PC with conventional lids. That's why in the photo you can see one bowl's tinfoil is stepped in. It's a tight fit.
I've taken a batch of 2 out already and have had no issues with the water getting in.
... Not ideal, I know...
"Not ideal" is an understatement... it's more like downright crap practice and I'd be amazed if that ended up well. I'd rather not do bottle grows if I had to do that, but it's your grow, do as you wish.
For example, every time I read here that the lids of the grain jars remain sterile after the pc cycle until we remove the foil I'm all like...
 The picture is horrible. But what you're seeing here is a fucking mold LC, the water is kinda yellow and the blob you see in the middle is mold mycelium.
That's a little plastic container with a snap-on lid that closes tight and a tiny hole covered with several layers of MP tape.
Some tap water was sterilized in it and the whole container was thourougly wrapped in foil before the cycle. I do this because I like to dip swabs in sterile water before streaking agar plates with them.
This was an spare container with water that I didn't use in the end and kept it wrapped in foil (with the lid closed of course) in a cupboard for several weeks. I took it to wash it the other day and surprise, surprise!
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Germs
Space Force


Registered: 06/26/11
Posts: 4,607
Loc: Texas
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Re: Updated Bottle Tek New and improved [Re: Josex]
#24784333 - 11/15/17 09:12 AM (6 years, 5 months ago) |
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Joining the bottle club here soon
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Mateja



Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: Updated Bottle Tek New and improved [Re: Josex]
#24784339 - 11/15/17 09:18 AM (6 years, 5 months ago) |
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Quote:
Josex said:
Quote:
Munchauzen said: is there a lid under the foil?
Quote:
Spindlymass said: No. Hahaha!
For 2 reasons :
1. The lids are LDPE 4 and won't withstand the 2 hour pressure cooking
2. The pyrex bowls won't fit one on top of the other in my little PC with conventional lids. That's why in the photo you can see one bowl's tinfoil is stepped in. It's a tight fit.
I've taken a batch of 2 out already and have had no issues with the water getting in.
... Not ideal, I know...
"Not ideal" is an understatement... it's more like downright crap practice and I'd be amazed if that ended up well. I'd rather not do bottle grows if I had to do that, but it's your grow, do as you wish.
For example, every time I read here that the lids of the grain jars remain sterile after the pc cycle until we remove the foil I'm all like...
 The picture is horrible. But what you're seeing here is a fucking mold LC, the water is kinda yellow and the blob you see in the middle is mold mycelium.
That's a little plastic container with a snap-on lid that closes tight and a tiny hole covered with several layers of MP tape.
Some tap water was sterilized in it and the whole container was thourougly wrapped in foil before the cycle. I do this because I like to dip swabs in sterile water before streaking agar plates with them.
This was an spare container with water that I didn't use in the end and kept it wrapped in foil (with the lid closed of course) in a cupboard for several weeks. I took it to wash it the other day and surprise, surprise!
Sterilized water getting moldy? And there's only water in the jar? I don't understand..
-------------------- Cakes inside Water Tub
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,998
Loc:
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Re: Updated Bottle Tek New and improved [Re: Mateja]
#24784378 - 11/15/17 09:33 AM (6 years, 5 months ago) |
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Tap water is all it takes for spores to germinate, even distilled sometimes. Try doing a spore syringe with tap water and if you keep the syringe long enough the spores will germinate, nothing wrong with that though, you can still use that syringe. The minerals in the water and maybe some particles that got in the water before sterilization are all it takes for spores to germinate.
Mold spores are so ubiquitous and tiny that they can even get inside syringes with only distilled and sterilized water in them if you keep them for months in a random place like a table in open air, and they'll germinate inside the syringe no problem. Again, I had a huge spore load at my place until recently, but nonetheless it can give you an idea how slippery and treacherous mold spores can be.
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Mateja



Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: Updated Bottle Tek New and improved [Re: Josex]
#24784442 - 11/15/17 10:02 AM (6 years, 5 months ago) |
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Hmm did not know this, thanks for sharing! Also I have nice growth in my LC will post pics soon in a day or two to bump your thread
-------------------- Cakes inside Water Tub
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Spindlymass
wafer thin.



Registered: 09/25/17
Posts: 957
Loc:
Last seen: 1 month, 12 days
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Re: Updated Bottle Tek New and improved [Re: Josex]
#24784688 - 11/15/17 12:22 PM (6 years, 5 months ago) |
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Quote:
Josex said:
Quote:
Munchauzen said: is there a lid under the foil?
Quote:
Spindlymass said: No. Hahaha!
For 2 reasons :
1. The lids are LDPE 4 and won't withstand the 2 hour pressure cooking
2. The pyrex bowls won't fit one on top of the other in my little PC with conventional lids. That's why in the photo you can see one bowl's tinfoil is stepped in. It's a tight fit.
I've taken a batch of 2 out already and have had no issues with the water getting in.
... Not ideal, I know...
"Not ideal" is an understatement... it's more like downright crap practice and I'd be amazed if that ended up well. I'd rather not do bottle grows if I had to do that, but it's your grow, do as you wish.
For example, every time I read here that the lids of the grain jars remain sterile after the pc cycle until we remove the foil I'm all like...
 The picture is horrible. But what you're seeing here is a fucking mold LC, the water is kinda yellow and the blob you see in the middle is mold mycelium.
That's a little plastic container with a snap-on lid that closes tight and a tiny hole covered with several layers of MP tape.
Some tap water was sterilized in it and the whole container was thourougly wrapped in foil before the cycle. I do this because I like to dip swabs in sterile water before streaking agar plates with them.
This was an spare container with water that I didn't use in the end and kept it wrapped in foil (with the lid closed of course) in a cupboard for several weeks. I took it to wash it the other day and surprise, surprise!
You sir, are correct. I realize I am waaaaaay out of my league in this thread. In retrospect, I shouldn't have even posted my noob stuff here.
But, since I have, I'm certainly grateful someone such as yourself has responed. I plan to continue growing and am just looking to learn and contribute to the community. I've learned a lot from folks such as yourself and appreciate you taking the time to respond.
I'm sure blown away that molds could make it into syringes and containers as you've described.
Edit: would you care to elaborate, for my benefit, on "crap practice"? Specifically the extra transfer in the SAB?
***So, Allof my jars went for shit, except one. Which you ask? The one I pressure cooked by itself . Lesson learned, got a presto 23 qt. and haven't looked back.
-------------------- enough knowledge to get into trouble. Not nearly enough to get out again.
Edited by Spindlymass (11/27/17 05:48 PM)
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