Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Injection Grain Bag   PhytoExtractum Buy Bali Kratom Powder   Left Coast Kratom Buy Kratom Extract   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Mushroom-Hut Liquid Cultures

Jump to first unread post Pages: < First | < Back | 288 | 289 | 290 | 291 | 292 | 293 | 294 | 295 | 296 | 297 | 298 | 299 | 300 | 301 | 302 | 303 | 304 | 305 | 306 | 307 | 308 | Next > | Last >
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
InvisibleJHOVA
Post whore
Male User Gallery


Registered: 02/17/17
Posts: 4,727
Loc: Flag
Re: Pastywhyte's Easy Agar Tek [Re: MadHatter333]
    #24762623 - 11/05/17 09:44 PM (6 years, 3 months ago)

Quote:

MadHatter333 said:
Thanks Herren I’ll look into doing that once I get started. I already have a coffee grinder so I will be set on that. Just have to get all the other material. What is better Karo or Honey? I don’t ever use Karo so I feel it might be a waste, but I love honey and would use it for food stuff as well. Is it okay to use the honey I buy for both myco Work and food stuff or should I have separate honey? I don’t think it would matter much after a PC run just better to be safe and ask.




A lot of people have forgotten to add karo to no ill effect. I've skipped it by accident numerous times.


--------------------
πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ


Extras: Filter Print Post Top
OfflineSteevo
Just a cog in the death wheel
 User Gallery


Registered: 03/02/17
Posts: 2,702
Loc: Here and There
Last seen: 1 year, 10 months
Re: Pastywhyte's Easy Agar Tek [Re: JHOVA]
    #24763089 - 11/06/17 06:04 AM (6 years, 3 months ago)

I put a hole in the side of those big lots containers. I've also kept some cultures going for months though for stones or pins


--------------------


Extras: Filter Print Post Top
InvisibleMadHatter333
We Are All Mad Here


Registered: 09/20/17
Posts: 4,650
Loc: Your Mom’s Rabbit Hole
Re: Pastywhyte's Easy Agar Tek [Re: JHOVA]
    #24763457 - 11/06/17 09:32 AM (6 years, 3 months ago)

Good to know Saturn I’ll keep that in mind. If it helps at all though I’d like to use it.


--------------------

TEKs I Like


Extras: Filter Print Post Top
Offlinepixelpopper
Crap Artist


Registered: 09/20/13
Posts: 4,022
Loc: Dreamland
Last seen: 4 months, 5 days
Re: Pastywhyte's Easy Agar Tek [Re: JHOVA]
    #24763540 - 11/06/17 10:13 AM (6 years, 3 months ago)

Quote:

Jonhova said:
Quote:

MadHatter333 said:
Thanks Herren I’ll look into doing that once I get started. I already have a coffee grinder so I will be set on that. Just have to get all the other material. What is better Karo or Honey? I don’t ever use Karo so I feel it might be a waste, but I love honey and would use it for food stuff as well. Is it okay to use the honey I buy for both myco Work and food stuff or should I have separate honey? I don’t think it would matter much after a PC run just better to be safe and ask.




A lot of people have forgotten to add karo to no ill effect. I've skipped it by accident numerous times.




For clarification, are you saying you didn't use any karo or honey? Or did you still use honey? Cus its an either/or ingredient rather than both


Extras: Filter Print Post Top
Invisibleherrenvolk


Registered: 05/14/16
Posts: 222
Re: Pastywhyte's Easy Agar Tek [Re: pixelpopper]
    #24763862 - 11/06/17 01:02 PM (6 years, 3 months ago)

Quote:

pixelpopper said:
Quote:

Jonhova said:
Quote:

MadHatter333 said:
Thanks Herren I’ll look into doing that once I get started. I already have a coffee grinder so I will be set on that. Just have to get all the other material. What is better Karo or Honey? I don’t ever use Karo so I feel it might be a waste, but I love honey and would use it for food stuff as well. Is it okay to use the honey I buy for both myco Work and food stuff or should I have separate honey? I don’t think it would matter much after a PC run just better to be safe and ask.




A lot of people have forgotten to add karo to no ill effect. I've skipped it by accident numerous times.




For clarification, are you saying you didn't use any karo or honey? Or did you still use honey? Cus its an either/or ingredient rather than both




He probably means that he omitted any sugars, and only put agar and potatoe.


MadHatter, most people use Karo light corn syrup, I think. But I've only ever used Walmart Great Value brand light corn syrup (lol) for my PDA agar. Honey should work fine as well, though some people avoid it because of its supposed antifungal properties.

Between the two, I would choose Karo, but if you already have honey and don't have Karo, then you may as well give it a try. It'll probably be fine.


Extras: Filter Print Post Top
InvisibleJHOVA
Post whore
Male User Gallery


Registered: 02/17/17
Posts: 4,727
Loc: Flag
Re: Pastywhyte's Easy Agar Tek [Re: herrenvolk]
    #24763871 - 11/06/17 01:07 PM (6 years, 3 months ago)

I skipped out on both honey and karo when i used it.


--------------------
πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ


Extras: Filter Print Post Top
InvisibleMadHatter333
We Are All Mad Here


Registered: 09/20/17
Posts: 4,650
Loc: Your Mom’s Rabbit Hole
Re: Pastywhyte's Easy Agar Tek [Re: JHOVA]
    #24764027 - 11/06/17 02:14 PM (6 years, 3 months ago)

I think I’ll go with the Karo if it’s what most people use. It’s not to expensive anyway and I’m sure I can find some other uses for it. Guess it’s not that important but if it gives me a edge why not? Thanks again guys. Look forward to starting my agar adventure!


--------------------

TEKs I Like


Extras: Filter Print Post Top
InvisibleJHOVA
Post whore
Male User Gallery


Registered: 02/17/17
Posts: 4,727
Loc: Flag
Re: Pastywhyte's Easy Agar Tek [Re: MadHatter333]
    #24764048 - 11/06/17 02:23 PM (6 years, 3 months ago)

IME the opposite is true. Less nutrition has lead to zhizomorphic growth.

making it much easier to distinguish from foreign growth on plates.

Try using it both ways so you can see for yourself.


--------------------
πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,811
Loc: Canada Flag
Trusted Cultivator
Re: Pastywhyte's Easy Agar Tek [Re: JHOVA]
    #24764078 - 11/06/17 02:35 PM (6 years, 3 months ago)

I used honey when i first started no pour agar. Works fine.


Extras: Filter Print Post Top
InvisibleMadHatter333
We Are All Mad Here


Registered: 09/20/17
Posts: 4,650
Loc: Your Mom’s Rabbit Hole
Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
    #24764229 - 11/06/17 03:25 PM (6 years, 3 months ago)

I’ll try honey then and maybe some without just to test it.


--------------------

TEKs I Like


Extras: Filter Print Post Top
Offlinewilspeak
Strong Myz
Male User Gallery


Registered: 01/25/17
Posts: 395
Last seen: 1 year, 7 days
Re: Pasty Agar Tek [Re: teknix]
    #24766471 - 11/07/17 01:13 PM (6 years, 3 months ago)

Quote:

Pastywhyte said:
ientific-Plastic-60x15mm-Sterile/dp/B005Z4QU5U/ref=sr_1_1?ie=UTF8&qid=1385949104&sr=8-1&keywords=petri+dish&ie=UTF8&linkCode=ll1]http://www.amazon.com/Karter-Scientific-Plastic-60x15mm-Sterile/dp/B005Z4QU5U/ref=sr_1_1?ie=UTF8&qid=1385949104&sr=8-1&keywords=petri+dish[/url]

Hello Pastywhyte, I brought some small mini rounds similar to the ones in your pictures, how do you keep them from melting in the pressure cooker? and is the hole that you made in the top use as a air hole and inoculation hole? Thank You


--------------------
EACH ONE TEACH ONE  "Some are tools of knowledge, some are tools of power. Some are tools For power. Some are for redundancy in diverse climates mushrooms do it all."-Kilindi lyi


Extras: Filter Print Post Top
InvisibleLeftfield420
bong toker
Male


Registered: 02/26/16
Posts: 10,055
Re: Pasty Agar Tek [Re: wilspeak]
    #24767090 - 11/07/17 05:15 PM (6 years, 3 months ago)

If your containers are pp5 they won't melt... And that hole is to allow for air to escape the container during pressure cooking, with no hole the containers warp or they get crushed by the built up pressure inside the pressure cooker


Extras: Filter Print Post Top
InvisibleInocuole
Scalpel of Evil's Bane
 User Gallery

Folding@home Statistics
Registered: 11/21/11
Posts: 24,863
Loc: β˜…
Trusted Cultivator
Re: Pasty Agar Tek [Re: wilspeak]
    #24768003 - 11/08/17 12:07 AM (6 years, 3 months ago)

Quote:

wilspeak said:
Hello Pastywhyte, I brought some small mini rounds similar to the ones in your pictures, how do you keep them from melting in the pressure cooker? and is the hole that you made in the top use as a air hole and inoculation hole? Thank You



If you're thinking agar plates have an inoculation hole then I highly recommend more reading..  And also the fact that he doesn't mention anything specific about keeping them from melting should be a clue.

https://www.shroomery.org/forums/showflat.php/Number/22721954


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


Extras: Filter Print Post Top
Offlinewilspeak
Strong Myz
Male User Gallery


Registered: 01/25/17
Posts: 395
Last seen: 1 year, 7 days
Re: Pasty Agar Tek [Re: Inocuole]
    #24768185 - 11/08/17 04:00 AM (6 years, 3 months ago)

Quote:

Inocuole said:
Quote:

wilspeak said:
Hello Pastywhyte, I brought some small mini rounds similar to the ones in your pictures, how do you keep them from melting in the pressure cooker? and is the hole that you made in the top use as a air hole and inoculation hole? Thank You



If you're thinking agar plates have an inoculation hole then I highly recommend more reading..  And also the fact that he doesn't mention anything specific about keeping them from melting should be a clue.

https://www.shroomery.org/forums/showflat.php/Number/22721954




Thank You


--------------------
EACH ONE TEACH ONE  "Some are tools of knowledge, some are tools of power. Some are tools For power. Some are for redundancy in diverse climates mushrooms do it all."-Kilindi lyi


Extras: Filter Print Post Top
Offlinewilspeak
Strong Myz
Male User Gallery


Registered: 01/25/17
Posts: 395
Last seen: 1 year, 7 days
Re: Pasty Agar Tek [Re: wilspeak]
    #24768189 - 11/08/17 04:03 AM (6 years, 3 months ago)

Quote:

wilspeak said:
Quote:

Inocuole said:
Quote:

wilspeak said:
Hello Pastywhyte, I brought some small mini rounds similar to the ones in your pictures, how do you keep them from melting in the pressure cooker? and is the hole that you made in the top use as a air hole and inoculation hole? Thank You



If you're thinking agar plates have an inoculation hole then I highly recommend more reading..  And also the fact that he doesn't mention anything specific about keeping them from melting should be a clue.

https://www.shroomery.org/forums/showflat.php/Number/22721954




Thank You




Hello Inocuole, I am New to this and I have to ask questions in order to help me understand how to do this. Thank You for the information.


--------------------
EACH ONE TEACH ONE  "Some are tools of knowledge, some are tools of power. Some are tools For power. Some are for redundancy in diverse climates mushrooms do it all."-Kilindi lyi


Extras: Filter Print Post Top
Offlinepixelpopper
Crap Artist


Registered: 09/20/13
Posts: 4,022
Loc: Dreamland
Last seen: 4 months, 5 days
Re: Pasty Agar Tek [Re: wilspeak]
    #24769350 - 11/08/17 01:43 PM (6 years, 3 months ago)

My first Pasty-plates are cooling off in the PC right now and I'm planning on trying my hand at cloning with them. This will be my first attempt at both cloning and agar.

If I've understood correctly, the best way to go about cloning is to select a good fruit, flame sterilize a scalpel and then cut some tissue from inside the stem in order to get a tissue sample that is a bit more sterile than the outside?

I've also seen it said to use the portion at the base of the stem or just under the cap.

I won't be using a pin as the fruits I will be selecting from are past pinning.


Extras: Filter Print Post Top
InvisibleLeftfield420
bong toker
Male


Registered: 02/26/16
Posts: 10,055
Re: Pasty Agar Tek [Re: pixelpopper]
    #24769570 - 11/08/17 03:37 PM (6 years, 3 months ago)

you want to pull the mushroom apart with your fingers then take your sterilized blade and cut a small tissue sample from the stipe and transfer that to agar


Edited by Leftfield420 (11/08/17 03:38 PM)


Extras: Filter Print Post Top
Offlinewilspeak
Strong Myz
Male User Gallery


Registered: 01/25/17
Posts: 395
Last seen: 1 year, 7 days
Re: Pasty Agar Tek [Re: Leftfield420]
    #24769861 - 11/08/17 06:08 PM (6 years, 3 months ago)

Quote:

Leftfield420 said:
you want to pull the mushroom apart with your fingers then take your sterilized blade and cut a small tissue sample from the stipe and transfer that to agar




I appreciate all the help and information that you have posted. I have some questions about THIS LINK >>>> https://www.shroomery.org/forums/showflat.php/Number/19208976/fpart/1/vc/1 ;   . My question is when and how are those plastic mini rounds in the pictures are inoculated with the spore syringe or shroom samples. I see when the holes are drilled in the mini rounds , but I dont understand how or when the spore samples are inoculated into the agar, can you explain that step. Thank You.


--------------------
EACH ONE TEACH ONE  "Some are tools of knowledge, some are tools of power. Some are tools For power. Some are for redundancy in diverse climates mushrooms do it all."-Kilindi lyi


Extras: Filter Print Post Top
InvisibleJHOVA
Post whore
Male User Gallery


Registered: 02/17/17
Posts: 4,727
Loc: Flag
Re: Pasty Agar Tek [Re: wilspeak]
    #24769879 - 11/08/17 06:13 PM (6 years, 3 months ago)

Quote:

wilspeak said:
Quote:

Leftfield420 said:
you want to pull the mushroom apart with your fingers then take your sterilized blade and cut a small tissue sample from the stipe and transfer that to agar




I appreciate all the help and information that you have posted. I have some questions about THIS LINK >>>> https://www.shroomery.org/forums/showflat.php/Number/19208976/fpart/1/vc/1 ;  . My question is when and how are those plastic mini rounds in the pictures are inoculated with the spore syringe or shroom samples. I see when the holes are drilled in the mini rounds , but I dont understand how or when the spore samples are inoculated into the agar, can you explain that step. Thank You.





Watch these


--------------------
πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ


Extras: Filter Print Post Top
InvisibleInocuole
Scalpel of Evil's Bane
 User Gallery

Folding@home Statistics
Registered: 11/21/11
Posts: 24,863
Loc: β˜…
Trusted Cultivator
Re: Pasty Agar Tek [Re: JHOVA]
    #24769905 - 11/08/17 06:21 PM (6 years, 3 months ago)

Can't even be bothered to write a unique post for each thread... pastes the link to this thread, in this thread. :lol:


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


Extras: Filter Print Post Top
Jump to top Pages: < First | < Back | 288 | 289 | 290 | 291 | 292 | 293 | 294 | 295 | 296 | 297 | 298 | 299 | 300 | 301 | 302 | 303 | 304 | 305 | 306 | 307 | 308 | Next > | Last >

Shop: North Spore Injection Grain Bag   PhytoExtractum Buy Bali Kratom Powder   Left Coast Kratom Buy Kratom Extract   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Mushroom-Hut Liquid Cultures


Similar ThreadsPosterViewsRepliesLast post
* Agar Tek Aristar 5,834 12 08/01/03 06:42 PM
by shirley knott
* ?NO,AGAR TEK AT SHROOMERY?? bluelou 5,750 8 12/13/19 06:16 PM
by mushboy
* Re: Peroxidated Agar Tek sparks 4,561 6 05/08/00 12:43 AM
by Anonymous
* agar tek? the man 2,777 4 12/16/02 10:45 AM
by the man
* NEW Tissue Culture Technique - No agar, No flow hood, easy as hell
( 1 2 all )
funkyballoon 21,324 38 05/11/01 11:08 AM
by MAIA
* Mycelium-only tek, Please Help! FaminedShaman 2,567 4 05/06/01 06:49 AM
by egghead
* Antibac Agar, DIY! superduper 4,871 13 12/04/02 02:20 PM
by superduper
* agar cultivation - easy! felixhigh 2,498 3 11/28/01 01:16 AM
by BBShrizz

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
797,484 topic views. 16 members, 109 guests and 27 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.035 seconds spending 0.012 seconds on 14 queries.