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Offlinel2en
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Spots in my cultures on agar
    #24759321 - 11/04/17 01:31 PM (7 years, 2 months ago)

I've got spots on the underside of my mycelium cultures. Two plates are LI test samples and one is from a spore print. Never encountered these. Is it a contam or something I should be concerned about? The cultures look and act healthy otherwise but would like a more trained eye to help me clear this up.

Yes, I already tried searching and could not find a duplicate problem.



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Offlinetacodude
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Re: Spots in my cultures on agar [Re: l2en]
    #24759361 - 11/04/17 01:48 PM (7 years, 2 months ago)

Those look like condensation.... Maybe it is air bubbles between the Shar and dish? That's just a guess and I'm not experienced, but it doesn't look like those spots are alive or anything to worry about.

Edit: looking closer again they are definitely air bubbles in the agar

Edited by tacodude (11/04/17 01:49 PM)

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InvisiblebodhisattaMDiscordReddit
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Re: Spots in my cultures on agar [Re: tacodude]
    #24760469 - 11/05/17 12:46 AM (7 years, 2 months ago)

I would make transfers

And test the LI on a jar or cake

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Offlinel2en
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Re: Spots in my cultures on agar [Re: bodhisatta]
    #24760530 - 11/05/17 01:57 AM (7 years, 2 months ago)

Quote:

bodhisatta said:
I would make transfers

And test the LI on a jar or cake




It's odd bc I test the LI syringes each time I pull em out of the fridge before I use it to noc a rye jar and this is the first time I seen those spots/growths.

I would've ruled the LI syringes as bad but I have the other plate with spore prints on their first transfer that did the same thing. In the same batch of Y plates, I have other LI samples and MS transfers (that were done at the same time as these ones pictured) that are perfect.

I'm stumped.

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InvisibleReblackah
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Re: Spots in my cultures on agar [Re: l2en]
    #24762891 - 11/06/17 01:04 AM (7 years, 2 months ago)

If it's the black flecks, could be something in your agar mix maybe? Does it appear as well on clean plates and was it there from the beginning?

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Offlinel2en
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Re: Spots in my cultures on agar [Re: Reblackah]
    #24775825 - 11/11/17 12:15 PM (7 years, 2 months ago)

The flecks are nutrient yeast. I am talking about the ring of dark circles in the middle of the cultures.

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InvisibleReblackah
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Re: Spots in my cultures on agar *DELETED* [Re: l2en]
    #24777037 - 11/12/17 02:15 AM (7 years, 2 months ago)

Post deleted by LizardWizard

Reason for deletion: ok

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Offlinel2en
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Re: Spots in my cultures on agar [Re: Reblackah]
    #24871488 - 12/27/17 12:32 PM (7 years, 21 days ago)

Quote:

LizardWizard said:
Is that a Pan Cyan culture? Or just a Cube culture? Or something other than those?




Cube --Panama Variety

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InvisibleReblackah
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Re: Spots in my cultures on agar [Re: l2en]
    #24871664 - 12/27/17 02:30 PM (7 years, 21 days ago)

Well, for a cube, I'd say you're fucked, but I wouldn't know what the hell is going on so I'm not sure either... It's colored, right? Not just shining through less because of culture density?

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