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l2en
Stranger



Registered: 08/10/17
Posts: 11
Last seen: 3 years, 3 months
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Spots in my cultures on agar
#24759321 - 11/04/17 01:31 PM (7 years, 2 months ago) |
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I've got spots on the underside of my mycelium cultures. Two plates are LI test samples and one is from a spore print. Never encountered these. Is it a contam or something I should be concerned about? The cultures look and act healthy otherwise but would like a more trained eye to help me clear this up.
Yes, I already tried searching and could not find a duplicate problem.
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tacodude
Old Soul

Registered: 07/20/10
Posts: 1,753
Last seen: 3 years, 7 months
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Re: Spots in my cultures on agar [Re: l2en]
#24759361 - 11/04/17 01:48 PM (7 years, 2 months ago) |
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Those look like condensation.... Maybe it is air bubbles between the Shar and dish? That's just a guess and I'm not experienced, but it doesn't look like those spots are alive or anything to worry about.
Edit: looking closer again they are definitely air bubbles in the agar
Edited by tacodude (11/04/17 01:49 PM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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Re: Spots in my cultures on agar [Re: tacodude]
#24760469 - 11/05/17 12:46 AM (7 years, 2 months ago) |
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I would make transfers
And test the LI on a jar or cake
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l2en
Stranger



Registered: 08/10/17
Posts: 11
Last seen: 3 years, 3 months
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Re: Spots in my cultures on agar [Re: bodhisatta]
#24760530 - 11/05/17 01:57 AM (7 years, 2 months ago) |
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Quote:
bodhisatta said: I would make transfers
And test the LI on a jar or cake
It's odd bc I test the LI syringes each time I pull em out of the fridge before I use it to noc a rye jar and this is the first time I seen those spots/growths.
I would've ruled the LI syringes as bad but I have the other plate with spore prints on their first transfer that did the same thing. In the same batch of Y plates, I have other LI samples and MS transfers (that were done at the same time as these ones pictured) that are perfect.
I'm stumped.
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Reblackah


Registered: 01/07/15
Posts: 13,770
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Re: Spots in my cultures on agar [Re: l2en]
#24762891 - 11/06/17 01:04 AM (7 years, 2 months ago) |
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If it's the black flecks, could be something in your agar mix maybe? Does it appear as well on clean plates and was it there from the beginning?
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l2en
Stranger



Registered: 08/10/17
Posts: 11
Last seen: 3 years, 3 months
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Re: Spots in my cultures on agar [Re: Reblackah]
#24775825 - 11/11/17 12:15 PM (7 years, 2 months ago) |
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The flecks are nutrient yeast. I am talking about the ring of dark circles in the middle of the cultures.
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Reblackah


Registered: 01/07/15
Posts: 13,770
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Re: Spots in my cultures on agar *DELETED* [Re: l2en]
#24777037 - 11/12/17 02:15 AM (7 years, 2 months ago) |
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Post deleted by LizardWizard
Reason for deletion: ok
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l2en
Stranger



Registered: 08/10/17
Posts: 11
Last seen: 3 years, 3 months
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Re: Spots in my cultures on agar [Re: Reblackah]
#24871488 - 12/27/17 12:32 PM (7 years, 21 days ago) |
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Quote:
LizardWizard said: Is that a Pan Cyan culture? Or just a Cube culture? Or something other than those?
Cube --Panama Variety
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Reblackah


Registered: 01/07/15
Posts: 13,770
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Re: Spots in my cultures on agar [Re: l2en]
#24871664 - 12/27/17 02:30 PM (7 years, 21 days ago) |
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Well, for a cube, I'd say you're fucked, but I wouldn't know what the hell is going on so I'm not sure either... It's colored, right? Not just shining through less because of culture density?
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