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 mushboy 
modboy
Registered: 04/24/05
Posts: 32,776
Loc: where?
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 Re: The Biopsy Method: A different way of doing LC.  [Re: Josex]
#24746157 - 10/29/17 06:27 PM (6 years, 5 months ago) |
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ill post pics of my soon to be fruiting shoeboxes tomorrow
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bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: The Biopsy Method: A different way of doing LC. [Re: mushboy]
#24746186 - 10/29/17 06:36 PM (6 years, 5 months ago) |
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What the hell ill give it a try too next time around. I like it. Though big wedges to my LC jar with some glass in the bottom and they're ready to use 3+4 days later with one agitation per day. I still like the idea behind this. Nice one josex
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Josex
#cheat_code
Registered: 11/13/15
Posts: 8,996
Loc: 
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Re: The Biopsy Method: A different way of doing LC. [Re: bodhisatta]
#24746568 - 10/29/17 09:51 PM (6 years, 5 months ago) |
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 cronicr
Registered: 08/07/11
Posts: 61,436
Loc: Van Isle 
Last seen: 2 years, 2 months
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
#24746752 - 10/30/17 12:49 AM (6 years, 5 months ago) |
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Quote:
Josex said:
Wow, just read Creamcorn's tek, just wow! haha
How in the actual fuck can someone manage to pull that off without contaminating the LC? 
It's almost offensive to compare it with this Ham. 
This tek is not about taking a perfectly visible core sample either but about inoculating with literally microscopic pieces of tissue from a clean agar culture to make the procedure as safe as possible, so you can perfectly get away with no testing and dicking around.
Also, I would not do this method poking an invitro agar pin as Cron said, I'd transfer the pin to a blank first. My confidence in pp5 containers with snap-on lids has shaken quite a bit after my last mold disaster a few months back. They're far from perfect and shit can def get in them if you keep them for long till they pin.
I dont actually poke the pin...i drop the whole thing in
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   It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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mushboy
modboy
Registered: 04/24/05
Posts: 32,776
Loc: where?
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Re: The Biopsy Method: A different way of doing LC. [Re: cronicr] 2
#24747874 - 10/30/17 02:50 PM (6 years, 5 months ago) |
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3quarts to 3 quarts in a 20qt mini
random shoebox
poured 6qt bag of oats. my first bag to not tam out.
pouring LCs into bags kicks ass
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Pipefitter537
You're Not Yelping
Registered: 11/25/16
Posts: 715
Loc: SodoSopa
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Re: The Biopsy Method: A different way of doing LC. [Re: mushboy]
#24748357 - 10/30/17 06:56 PM (6 years, 5 months ago) |
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Good shit Josex.
Very well written and explained.
Will give this a try soon.
Thanks for sharing
Mushboy
Shits looking good dude. Glad it is working for ya. Keep it up man.
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"You should never underestimate the predictability of stupidity"-Bullet Tooth Tony
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Snazz
Polymath
Registered: 11/24/15
Posts: 1,584
Loc: Canada
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Re: The Biopsy Method: A different way of doing LC. [Re: Pipefitter537]
#24748414 - 10/30/17 07:24 PM (6 years, 5 months ago) |
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So, I had a transfer that landed with a 45° angle on top face. With nice ropey strands. 5 days in it's radial but the top launched up with 2 twisted pair looking strands about 4cm long into the air.
Decided to use flamed tweezers and try an LC with the strands. Looking decent so far. Inspired by this topic. Thx 
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senseit
Listening
Registered: 01/16/16
Posts: 156
Last seen: 7 months, 17 days
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Re: The Biopsy Method: A different way of doing LC. [Re: Snazz]
#24748492 - 10/30/17 08:00 PM (6 years, 5 months ago) |
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Quote:
Josex said:
What I currently do is pour the whole LC into a PP5 blender bottle like this one:
Pouring the whole thing into a bottle is not a vector if you ask me, been doing it a lot with success for months.
Cool tek! I just made up a jar yesterday, we'll see how it goes.
Question: Is there a reason not to grow out the LC directly in the pp5 blender bottle? Then you wouldn't even have to transfer it from the glass to the bottle.
Thanks!
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Josex
#cheat_code
Registered: 11/13/15
Posts: 8,996
Loc:
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Re: The Biopsy Method: A different way of doing LC. [Re: senseit] 1
#24748958 - 10/31/17 12:53 AM (6 years, 5 months ago) |
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Quote:
senseit said:
Question: Is there a reason not to grow out the LC directly in the pp5 blender bottle? Then you wouldn't even have to transfer it from the glass to the bottle.
Because you can't see through the bottle. Even if it was clear plastic, visibility would be horrible.
The idea of pouring the LC into the bottle can be intimidating at first, and you don't need to do it if it makes you nervous.
I only use it when I make a 400-500ml LC to inoculate lots of jars, enough that they won't fit all in the SAB.
If you plan on doing lots of jars in one go, then yes, the bottle comes in handy. Or you could also make several smaller LC's to inoculate lots of jars in the same session without having to use a blender bottle. Whatever makes you feel more comfortable.
Edited by Josex (10/31/17 04:03 AM)
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mushboy
modboy
Registered: 04/24/05
Posts: 32,776
Loc: where?
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex] 2
#24749949 - 10/31/17 01:11 PM (6 years, 5 months ago) |
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ive been using quart jars with my usual tyvek mp tape filter.
500ml of liquid in a quart still leaves plenty of headroom to stir up the broth w/out getting the filter wet.
and its super easy to pour. but i like the idea of the really small spout to pour from. less exposure=less chance of ickies.
FIRST LC KNOTS.
cant see any but ya know.. they dont show up with my shitty nokia fone.
yah dude. i got 3 more quarts to spawn tonight.. and that bag should be done by the weekend. mono town
..and ive done about 3 rounds of this. lots of jars. lots of broth. no contams
now pimpin is easy 
Edited by mushboy (10/31/17 01:16 PM)
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Josex
#cheat_code
Registered: 11/13/15
Posts: 8,996
Loc:
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Re: The Biopsy Method: A different way of doing LC. [Re: mushboy] 1
#24749996 - 10/31/17 01:41 PM (6 years, 5 months ago) |
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Nice!
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tombosley8
Full on... Bossley Baggins
Registered: 10/14/13
Posts: 3,660
Last seen: 10 months, 15 days
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex] 1
#24750001 - 10/31/17 01:44 PM (6 years, 5 months ago) |
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Yeah mushboy Rockin IT!
Can't wait to try 
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Fungus Mountain
Poke-N-Squirt
Registered: 10/16/14
Posts: 1,188
Loc: Front row @ the Freakshow
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Re: The Biopsy Method: A different way of doing LC. [Re: tombosley8] 1
#24750204 - 10/31/17 03:51 PM (6 years, 5 months ago) |
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Nice write-up. Simple and straight forward. Will give this a shot for sure.
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“Until they became conscious they will never rebel, and until after they have rebelled they cannot become conscious.”
― George Orwell, 1984
"If you can't explain it simply, you don't understand it well enough."
― Albert Einstein
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Josex
#cheat_code
Registered: 11/13/15
Posts: 8,996
Loc:
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Re: The Biopsy Method: A different way of doing LC. [Re: Fungus Mountain] 1
#24750715 - 10/31/17 08:09 PM (6 years, 5 months ago) |
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I updated the OP with a simple test to know if a culture is clean. Don't want any of you to fuck this up.
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ComebackKid
Multispore Enthusiast
Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex] 1
#24752692 - 11/01/17 04:47 PM (6 years, 5 months ago) |
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Didn't have any metal lids with SFDs so I'm going unmodified. I'll let you know how she goes
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 Substrate surface conditions / Monotub prep and care 
  
Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.
Our individual experiences are all part of the universe's experience of itself
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Josex
#cheat_code
Registered: 11/13/15
Posts: 8,996
Loc:
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Re: The Biopsy Method: A different way of doing LC. [Re: ComebackKid]
#24752727 - 11/01/17 05:06 PM (6 years, 5 months ago) |
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As far as GE is concerned you'll be fine. I really think you can even screw the lid all the way during colonization, LC's don't need that much GE, if at all.
The part that sketches me a little is having to unscrew a bulky plastic lid to inoculate the broth, but then again I'm a paranoid fuck when it comes to noccing LC's.
We'll see how that goes
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Josex
#cheat_code
Registered: 11/13/15
Posts: 8,996
Loc:
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
#24752814 - 11/01/17 05:44 PM (6 years, 5 months ago) |
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Hey CBK, I think I remember you posting some nice wide pp5 tubes with screw cap, right? If you still have them around you can use them to mod a lid for LC's for easy inoculation and GE. I'm not home right now, but can tell you later if you want.
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ComebackKid
Multispore Enthusiast
Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
#24752885 - 11/01/17 06:17 PM (6 years, 5 months ago) |
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I have the metal lid unmodified and upside down. I've had issues with LC and plastic lids in the past.
I'm not actually sure what you're talking about tbh. Ziplock twistnloc?
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.
Our individual experiences are all part of the universe's experience of itself
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Josex
#cheat_code
Registered: 11/13/15
Posts: 8,996
Loc:
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Re: The Biopsy Method: A different way of doing LC. [Re: ComebackKid]
#24752901 - 11/01/17 06:23 PM (6 years, 5 months ago) |
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Oh well I thought we were talking plastic lids hehe, forget about it.
I was referring to the test tubes or slants you posted long ago in POTD, but using unmodified metal lids is fine, so let's pretend I didn't say anything lol
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van hatton
Still a noob
Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
#24752912 - 11/01/17 06:31 PM (6 years, 5 months ago) |
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Pcing some syringes and brf plates right meow
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If I ever give out misinformation please inform me so I can have the correct information.
Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.
Caps McGee said:
Fun part is figuring out what works best for you
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