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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: Mateja]
    #24769418 - 11/08/17 04:13 PM (3 years, 11 months ago)

Good luck dude, the results are gonna be interesting, no matter if you fail or succeed. I hope it's the latter. :popcorn:


--------------------
LC's are easy
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Clean yo shit
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24769454 - 11/08/17 04:32 PM (3 years, 11 months ago)

I don't see anything wrong with my sterile procedure, my only concern would be if the SHIP density could affect the tissue on/inside the needle. We'll see :smile:


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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24769461 - 11/08/17 04:35 PM (3 years, 11 months ago)

Quote:

Josex said:
You mean grain water like in the op? Nasty sediment and all? That could clog a syringe fast and furious I think.




Oh god I just made 10 jars of the grain LC with sediment. I have to use syringe to inoculate my bags with LC since I don't have a flowhood and they are presealed. LOL! :facepalm:


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Re: The Biopsy Method: A different way of doing LC. [Re: herrenvolk]
    #24769488 - 11/08/17 04:54 PM (3 years, 11 months ago)

Quote:

Mateah said:
I don't see anything wrong with my sterile procedure, my only concern would be if the SHIP density could affect the tissue on/inside the needle. We'll see :smile:



Yeah that was in my mind too, but I don't think it will be an issue, it's the sharp edge of the needle that makes its way through the ship, the interior of the needle will be fine.
Quote:

herrenvolk said:
Quote:

Josex said:
You mean grain water like in the op? Nasty sediment and all? That could clog a syringe fast and furious I think.




Oh god I just made 10 jars of the grain LC with sediment. I have to use syringe to inoculate my bags with LC since I don't have a flowhood and they are presealed. LOL! :facepalm:



Just try not to disturb the sediment, you'll lose some goodies and some myc at the bottom though.
Or you could just pour the damn thing :cool:


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
LAGM '21

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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24774211 - 11/10/17 07:18 PM (3 years, 11 months ago)

Well even the ones I was concerned about grew. This is 10 days in shaken once a day.



Time to test em


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Re: The Biopsy Method: A different way of doing LC. [Re: bodhisatta]
    #24774396 - 11/10/17 08:43 PM (3 years, 11 months ago)

That's perfectly reassuring. I'm gonna do syrige agar pokes for my next round of grain petris culture hunting.


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Re: The Biopsy Method: A different way of doing LC. [Re: Violet]
    #24774509 - 11/10/17 09:34 PM (3 years, 11 months ago)

:mushroom2:
Im digging it so far


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Re: The Biopsy Method: A different way of doing LC. [Re: bodhisatta]
    #24775058 - 11/11/17 05:27 AM (3 years, 11 months ago)

Quote:

Violet said:
That's perfectly reassuring. I'm gonna do syrige agar pokes for my next round of grain petris culture hunting.



So much fun trying out new methods :smile:


Quote:

bodhisatta said:
:mushroom2:
Im digging it so far



:dancer:


Two days now, I notice growth as well  :rockon:


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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: Mateja] * 2
    #24775309 - 11/11/17 09:54 AM (3 years, 11 months ago)

Quote:

bodhisatta said:
Well even the ones I was concerned about grew. This is 10 days in shaken once a day.



Time to test em



Beer wort LC...  :homerdrool:

I updated the OP. You don't need to boil the water with the flour to make the broth. It's simpler than that:
Quote:

Josex said:
The prep:
  • Take a small handful of grains and grind that shit up into a powder. I use a coffee grinder.
  • Weigh out enough flour for the amount of water you want to use for one single LC. The ratio I use is 1 gram of flour for 500ml of water. Adjust accordingly.
  • Throw the powder into the LC jar and add the water (cold is fine).
  • Sterilize for 1 hour at 15 psi. Some may find this overkill but I prefer to be safe than sorry when dealing with grains. Extended pc times for this does not hurt anything at all.





I've been boiling the water with the flour since I started doing this and just never gave it a second thought :shrug: I tried this way the other day and it's just the same.

Quote:

Mateah said:
Two days now, I notice growth as well  :rockon:



:highfive:
Nice, I did not see any reason for it not to work, it's just that inoculating through a SHIP has some drawbacks and it's not as safe.
This had me thinking... I'll have to update the op with another little trick for people who are just too paranoid about inoculating the LC and don't want to expose the sterile liquid to the air of the SAB at all, been there done that.
I have to take some pics and will update the op later. :thumbup:


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
LAGM '21

PHENO HUNTERS


Edited by Josex (11/11/17 10:13 AM)


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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24775680 - 11/11/17 12:53 PM (3 years, 11 months ago)

double postin from POTD but fukkit. i likes this methods me thinks. :takingnotes:


Quote:

mushboy said:
20qt mega shoebox.. or a mini mono. whichever nomenclature you prefer.



not bad for  ...uuurrmmmm... trich shrooms.:stoned:







not bad for just 2 myco qts wbs 2 quarts coco and a tad trich for flavor:popcorn:


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Re: The Biopsy Method: A different way of doing LC. [Re: mushboy]
    #24775727 - 11/11/17 01:20 PM (3 years, 11 months ago)

:etjesus:

That clone is a killer mang, like really, wanna see moaar!
Your jars looked very clean to me though, that's why I asked you what went wrong the other day :pm:


--------------------
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24775731 - 11/11/17 01:25 PM (3 years, 11 months ago)

i can only assume one of the quarts had something fall in during the inoculation.:shrug:

imo if it were the LC, everything would of been fucked. obviously not the case.
if it were in the coco?? maybe?? ecoearth does have weird shit inside but thats to easy.
or when i poured, something fell into the grain jar and since i shook it, the contam remained hidden???:confused:

usually when i fuck up an inoculation its clear as day because you can see it on the top of the grains.
but since this was LC and i shook the jars minutes after inoculation ...it remained buried.

ive got another 20qt with 3qts spawn.. a few shoeboxes and a 50-60qt mono. i staggered the spawning to keep shit exciting.

same clone. all open air.. gotta baby sit these bitches:rockon:


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Re: The Biopsy Method: A different way of doing LC. [Re: mushboy]
    #24775746 - 11/11/17 01:35 PM (3 years, 11 months ago)

Dud! Don't be such an LC noob. :crankeyclaus:

Leave the jar in peace after pouring man. Among other things, you could get the filter wet if you shake right after pouring.
I leave the jars in peace for 4 days and then come give 'em the first shake. At that point the bottom of the jar should be full of white myc and also some myc on the surface. After that first shake all goes fast. :thumbup:


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
LAGM '21

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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24775786 - 11/11/17 01:58 PM (3 years, 11 months ago)

hhaha lolz:facepalm:


ok well then lets document what i fucked up.. and it still worked awesomely.

i used 5g of grain per 500ml for the brooth
i shook wet jars
i waas tripping hardcore when i spawned them



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Re: The Biopsy Method: A different way of doing LC. [Re: mushboy]
    #24775797 - 11/11/17 02:01 PM (3 years, 11 months ago)

Gonna be trying this out on Monday! Been a few months since I’ve touched LC, was using Pasty’s EzLC with decent results but want to try something new. Thanks Josex

:manofapproval:


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Re: The Biopsy Method: A different way of doing LC. [Re: mushboy]
    #24775811 - 11/11/17 02:07 PM (3 years, 11 months ago)

:awelol:

Bright side is now you know better.  :strokebeard:
Ain't getting worse next time.


--------------------
LC's are easy
Go big with No-pours
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24775853 - 11/11/17 02:30 PM (3 years, 11 months ago)

Good luck Germs :cheers:


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24775885 - 11/11/17 02:47 PM (3 years, 11 months ago)

My 10/28 LC looks ready. Got two rye bags sterilizing right now. More fun to add to my growlogs.

Thx for the cool Tek


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Re: The Biopsy Method: A different way of doing LC. [Re: Snazz]
    #24776380 - 11/11/17 07:24 PM (3 years, 11 months ago)

Ok bitches! I updated the OP for those paranoid fucks out there that get sketched out by the idea of opening the lid to incoulate the broth.

Quote:

ALTERNATIVE WAY OF INOCULATING

I'll present another way of inoculating which is the one I used when I started doing this and still do sometimes, but to be honest, it's just easier to lift the lid to inoculate as mentioned above.
This way is especially good for people who feel a little paranoid about exposing the sterile broth to the air of the SAB,  peace of mind is priceless...
I must admit I was loath to include it in the 'official' version of this tek because it's a little embarrassing to admit how much of a paranoid fuck one can be.

I also did not include it as the main option because for this to work you will need a whole SFD covering the lid.
I'm aware not everybody can find SFD's easily (people from the EU need to import them from the US), so the first option I presented is kind of the standard way of inoculating that can be done with any type of lid for your LC jar, so you don't necessarily have to use SFD's.

Materials:
  • Metal lid and ring for mason jar, preferably regular mouth.
  • Whole synthetic filter disc.
  • A piece of foil, scissors and micropore tape.
  • A drill.
  • A thin drill bit to make a pilot hole, a thicker one to make a bigger hole and a grinding stone bit to make the final hole (~2/5 inches in diameter or 1cm).

  • A piece of wood.




Here's what you need to do:
  • Drill a big hole through the lid with the grinding stone bit (~1cm in diameter or 2/5 inches). It's better if you drill the hole next to the edge of the lid, it will facilitate the procedure later in the SAB.
    We used the smaller drill bits to make a pilot hole for the grinding stone bit. In the 2nd pic you can see the remainings of MP tape from previous LC's, shit won't quit no matter what, never mind.

  • Cut out a small square of foil but big enough to cover the hole.

  • Place the piece of foil over the hole and cover the foil with one layer of MP tape (you can use 2 layers but it's not necessary).

  • Mark the center of the hole with a sharpie. Trust me, it's going to be useful when it's time to inoculate.

  • We're going to be inoculating through the hole by barely poking this sterile makeshift inoculation port with the tip of the needle, so you can pc the LC jar placing the lid with the rubber side down no problem, since we're not going to open the lid.

  • Put the SFD on top of the lid and screw the ring back on.

Some may think that this way entails some risks, like for instance, mold spores can stick to the needle during the procedure and by poking the foil we might introduce them in the broth... To be honest, that ain't gonna happen even if you do this a thousand times and opening the lid to inoculate is also a risk, everything is a risk in this hobby. :sad:

Drilling the hole next to the edge will make the procedure much easier for you because you would only need to barely lift the SFD at a sharp angle to inoculate through the hole.

A useful tip if you're going to be doing this:

Before pc'ing the LC jar, align the hole with any distinguishable feature in the jar, be it the front of the jar where is says "Ball" or whatever.
This will allow you to know where the hole is before you inoculate.

Note: This is still a pour tek, as you will see later. Never, ever use this makeshift inoculation port to aspirate the LC.





--------------------
LC's are easy
Go big with No-pours
Clean yo shit
LAGM '21

PHENO HUNTERS


Edited by Josex (11/12/17 06:05 AM)


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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24778265 - 11/12/17 06:45 PM (3 years, 11 months ago)

Quote:

Josex said:
Quote:

bodhisatta said:
Well even the ones I was concerned about grew. This is 10 days in shaken once a day.



Time to test em



Beer wort LC...  :homerdrool:

I updated the OP. You don't need to boil the water with the flour to make the broth. It's simpler than that:
Quote:

Josex said:
The prep:
  • Take a small handful of grains and grind that shit up into a powder. I use a coffee grinder.
  • Weigh out enough flour for the amount of water you want to use for one single LC. The ratio I use is 1 gram of flour for 500ml of water. Adjust accordingly.
  • Throw the powder into the LC jar and add the water (cold is fine).
  • Sterilize for 1 hour at 15 psi. Some may find this overkill but I prefer to be safe than sorry when dealing with grains. Extended pc times for this does not hurt anything at all.





I've been boiling the water with the flour since I started doing this and just never gave it a second thought :shrug: I tried this way the other day and it's just the same.




That's how I prepped mine except I used spelt flour cause I'm a lazy fuck hahaha but it worked beautifully

I didn't shake till today, 8 days later, its looking good :thumbup:
Still some sediment floating around so I'm probably going to wait another week before I use it.

Also, I want to mention again that I didn't drill a hole in the lid. Unmodified metal lid placed upside down works beautifully



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