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InvisiblemushboyMDiscord
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Re: The Biopsy Method: A different way of doing LC. [Re: insanemike]
    #24766900 - 11/07/17 05:54 PM (3 years, 11 months ago)

no. i chop the base off to about an inch stump. then i pull the base apart like a bag of chips.. if that makes sense..

exposing making 2 halves. i then take the flamed blade and gently scrap out a sample.. put to agar. ... eat the discarded  specimen:stoned:


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OfflineSteevo
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Re: The Biopsy Method: A different way of doing LC. [Re: insanemike]
    #24766905 - 11/07/17 05:56 PM (3 years, 11 months ago)

Use your fingers to tear it apart and a scalpel to cut your sample from the center


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Invisibleinsanemike
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Re: The Biopsy Method: A different way of doing LC. [Re: Steevo]
    #24768765 - 11/08/17 11:51 AM (3 years, 11 months ago)

So this shouldn't be done in front of a flowhood because moving air is bound to blow whatever is on the outside of the stipe into the center while tearing it open? Also doesn't tearing cause cells to break free from the outside flesh and be flung into the air anyway?


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Re: The Biopsy Method: A different way of doing LC. [Re: insanemike]
    #24768769 - 11/08/17 11:52 AM (3 years, 11 months ago)

this tek is using agar not fruits for inoculating the lc


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InvisiblemushboyMDiscord
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Re: The Biopsy Method: A different way of doing LC. [Re: tombosley8]
    #24768792 - 11/08/17 12:02 PM (3 years, 11 months ago)

even though ive had clean clones on the first dish, id be way to scared to shot tissue into LC.

put to agar and then poke with the needle would be the way id go.  ...wait. i did go that route!


scraped out tissue from the guy on the left.

agar'ed


poked, LCed, poured, colonized, spawned


i should of cleaned up the original culture. but fuck it. it worked too easily to care. and the tiny specs
of myc from the poke slimmed down the genetics even more so maybe you dont need to use a super nice culture.


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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: mushboy]
    #24768812 - 11/08/17 12:08 PM (3 years, 11 months ago)

The slimming down of the genetics is a huge one I tell ya, it trumps the 'easy' aspect of noccing with the poke.


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Re: The Biopsy Method: A different way of doing LC. [Re: mushboy]
    #24768815 - 11/08/17 12:10 PM (3 years, 11 months ago)

What about poking a pin on agar?

Should be sterile


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Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


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InvisiblemushboyMDiscord
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Re: The Biopsy Method: A different way of doing LC. [Re: van hatton]
    #24768818 - 11/08/17 12:11 PM (3 years, 11 months ago)

Id just drop the pin zgzr


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Offlinetombosley8
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24768819 - 11/08/17 12:11 PM (3 years, 11 months ago)

damnit you guys are making me really want to try this.

I guess tomorrow is a good day!


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Re: The Biopsy Method: A different way of doing LC. [Re: mushboy]
    #24768837 - 11/08/17 12:14 PM (3 years, 11 months ago)

I mean I definitely would as well :lol: just a thought.


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If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: van hatton]
    #24768848 - 11/08/17 12:16 PM (3 years, 11 months ago)

Depends... If you've taken transfers from that plate, I would not risk it. If you work with snap-on pp5's, I wouldn't. If you use half pint jars or petris, maybe. But I'd personally shoot the pin tissue on agar first, no matter what. We're talking days till you can use that plate to inoculate.


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
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Re: The Biopsy Method: A different way of doing LC. [Re: insanemike]
    #24768852 - 11/08/17 12:17 PM (3 years, 11 months ago)

insanemike said:
How do you rip the stipe? I'm a little confused? You do not use a blade to pierce into the stipe?




Rip in half with your fingers and then use a flamed scalpel to take a nice clean sample from the interior of the stipe. If you cross cut, it will drag all the filth on the outside of the stipe into your sample area. At least that's how I did it....:smile:
Quote:



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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: CapnCrown]
    #24768890 - 11/08/17 12:27 PM (3 years, 11 months ago)

Also forgot to mention Van, sometimes it's just easier to transfer the whole pin to agar, them skinny pins are not that easy to poke sometimes. Then let the pin colonize the plate a bit and take a biopsy from the myc that grew from the base of the pin, as it is always more vigorous.


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
LAGM '21

PHENO HUNTERS


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InvisiblemushboyMDiscord
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24768906 - 11/08/17 12:30 PM (3 years, 11 months ago)



:cool:


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Re: The Biopsy Method: A different way of doing LC. [Re: mushboy]
    #24768928 - 11/08/17 12:38 PM (3 years, 11 months ago)

Is stipe tissue more vigorus than say LI/LC?


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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: JHOVA]
    #24768939 - 11/08/17 12:41 PM (3 years, 11 months ago)

Uh?


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
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InvisiblemushboyMDiscord
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24768945 - 11/08/17 12:42 PM (3 years, 11 months ago)

:derfase:

tissue is the inoculate. lc/li is a broth to grow/suspend said tissue

thats like asking if pancakes are better than maple syrup


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InvisiblebodhisattaMDiscordReddit
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Re: The Biopsy Method: A different way of doing LC. [Re: mushboy]
    #24768957 - 11/08/17 12:45 PM (3 years, 11 months ago)

Tires vs wheels


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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24768975 - 11/08/17 12:51 PM (3 years, 11 months ago)

Right. Well i saw eat colonize a 1/2 pint brf cake in 3-4 days using blended stipe tissue.
Is that faster or about the same as using LC/LI to inoculate a 1/2 pint brf cake?


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Re: The Biopsy Method: A different way of doing LC. [Re: bodhisatta]
    #24768977 - 11/08/17 12:51 PM (3 years, 11 months ago)



I know :cool:

Bad picture look 4 transfers 2 days ago.


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


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