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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: Sold Out Online]
    #27107017 - 12/25/20 09:43 AM (9 months, 28 days ago)

Nice man.

Quote:

Sold Out Online said:

Now I need to figure out how the hell I’m ever going to get through 1600ml of GT LC.




I get that every time, you feel you could inoculate the fucking world.
I usually end up using a small quantity from each LC and tossing the rest.


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
LAGM '21

PHENO HUNTERS


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OfflineSold Out Online
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #27107034 - 12/25/20 09:53 AM (9 months, 28 days ago)

It’s interesting that the one screwed one was LC2LC. Nothing I’ve poked has ever contaminated, and I’ve had a shitty run of mold on agar it took me forever to get away from.

This method is pretty fool proof.


--------------------


Threads that have been useful to me:
Clean Spawn checklist for new growers


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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #27113969 - 12/29/20 12:53 PM (9 months, 24 days ago)

Quote:

Josex said:
Have faith, child.

:pope:



Faith delivered :laugh:
This is Tampenensis.





They're looking quite healthy 5 days in.


Edited by CorteX_ (12/29/20 01:16 PM)


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OfflineA.k.a
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Re: The Biopsy Method: A different way of doing LC. [Re: CorteX_]
    #27114381 - 12/29/20 04:40 PM (9 months, 23 days ago)

Forgot to add this last week but this is Mexicana Jalisco about a month old.



Definitely makes some nice syringes. Im curious how it grows out now too cuz the myc from the poke is so small it’s gotta be like doing a few transfers worth of plate work isolating genetics.


--------------------
LAGM2020


Edited by A.k.a (12/29/20 04:42 PM)


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Offlinethe man
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Re: The Biopsy Method: A different way of doing LC. [Re: A.k.a]
    #27116331 - 12/30/20 03:30 PM (9 months, 22 days ago)

why not suck up some LC then take biopsy so squirt LC liquid instead of water back into LC?


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Re: The Biopsy Method: A different way of doing LC [Re: the man]
    #27117249 - 12/31/20 12:45 AM (9 months, 22 days ago)

also searched the thread has anyone tried innoculating through ship or rtv? as the talk was just about aspirating finished LC. if not i guess we will find out did some reishi and oysters will see how it works one had chunk of rtv so thats a good sign lol. guess gotta have faith. if that works it certainly is better then opening lids or poking through tinfoil and covering.

Also,

what may prove helpful is the biopsy needles as they have a 5 micron filter built in so if reusing the same syringe to do multi innoculations it would help nothing get back up into the syringe..obv doesnt seem to be an issue just an idea.

cheers!


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Re: The Biopsy Method: A different way of doing LC [Re: the man]
    #27117283 - 12/31/20 12:59 AM (9 months, 22 days ago)

Well if you already had an lc you wouldn’t need the biopsy right? Maybe I misunderstood.


The picture of the Jalisco I posted a few up was done through a ship.


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Re: The Biopsy Method: A different way of doing LC [Re: A.k.a]
    #27117292 - 12/31/20 01:07 AM (9 months, 22 days ago)

right. but not what i meant :wink:.  what i was wondering if action of taking a biospy then putting it through an rtv ship lid to inoculate the LC would be an issue.. ie stuck with rubber squished etc etc.  as seems much easier then OP had instructed so was curious if there was an actual reason for that and not using silicone to incolate LC with the biospy.

cheers

nm celestial said he did it in stone thread


Edited by the man (12/31/20 04:47 AM)


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OfflineCorteX_
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Re: The Biopsy Method: A different way of doing LC [Re: the man]
    #27117919 - 12/31/20 12:05 PM (9 months, 22 days ago)



7 days in now. I know you can never tell with LC, but I don't see how anything can go wrong at this point. Great tek, will use this one again in the future. Many thanks!


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Invisiblemicelio
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Re: The Biopsy Method: A different way of doing LC [Re: CorteX_]
    #27118010 - 12/31/20 12:49 PM (9 months, 22 days ago)

Nice picture...

How many pokes did you do?


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Re: The Biopsy Method: A different way of doing LC [Re: micelio]
    #27122886 - 01/02/21 11:39 PM (9 months, 19 days ago)

Just one small poke. Just wish I prepared the lid with 2 ships instead of polyfill in one hole. Galindoi is apparently really thick and hard to break up and I can't really shake.


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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC [Re: CorteX_]
    #27122890 - 01/02/21 11:41 PM (9 months, 19 days ago)

You need some kind of filter if you're planning on aspirating through a SHIP, so you're good. A whatman filter is a better option of course, but if you don't fill the jar a lot with liquid there should be plenty of room to swirl without wetting the poly.


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
LAGM '21

PHENO HUNTERS


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Re: The Biopsy Method: A different way of doing LC [Re: Josex]
    #27129153 - 01/05/21 09:40 PM (9 months, 16 days ago)

Alright, so I decided to dive into LC, came across this great way to start my adventure!  Sunday I took 4 plates, (2)huatla, (1)Malabar and (1)PE.  Gave em all a poke-n-shoot and began the waiting game.  Didn’t take any pictures, not much to see, but when I gave them a swirl, I can see a nice white-ish cyclone starting from bottom center and the jar comes alive, looking to be the start of some good shit I hope.  It’s a good thing some are willing to share their ways, thank you Josex!


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Re: The Biopsy Method: A different way of doing LC [Re: Jarhead3521]
    #27129165 - 01/05/21 09:51 PM (9 months, 16 days ago)

:headbanger:


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
LAGM '21

PHENO HUNTERS


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Re: The Biopsy Method: A different way of doing LC [Re: Josex]
    #27129340 - 01/05/21 11:31 PM (9 months, 16 days ago)

I had little faith i got a good poke with old dule needle as didnt see much just rtv.. but have seen teh light!! clouds from heaven! lol. great tek  less time and surface area = less contams.


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Re: The Biopsy Method: A different way of doing LC [Re: the man]
    #27133759 - 01/07/21 09:00 PM (9 months, 14 days ago)

OHHHHHHHH SHIT SON!  This makes me giddy, best 2 of 4 jars.  PE and Malabar coast.  The two huatla have some growth but not like these.



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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC [Re: Jarhead3521]
    #27133923 - 01/07/21 09:59 PM (9 months, 14 days ago)

:megusta:


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
LAGM '21

PHENO HUNTERS


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Re: The Biopsy Method: A different way of doing LC [Re: Josex]
    #27134060 - 01/07/21 10:51 PM (9 months, 14 days ago)

Quote:

Josex said:
:megusta:



I guess growth doesn’t show well in the picture?


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Re: The Biopsy Method: A different way of doing LC [Re: Jarhead3521]
    #27134078 - 01/07/21 11:04 PM (9 months, 14 days ago)

That sticker means "me gusta" = I like. Well sometimes it's not easy to take a good pic of an LC. I like to prep them lighter than that myself, 0,2% LME, it lets you see better.


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
LAGM '21

PHENO HUNTERS


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Re: The Biopsy Method: A different way of doing LC [Re: Josex]
    #27134089 - 01/07/21 11:09 PM (9 months, 14 days ago)

You can really see the exponential growth of the myc with this too.

Mine took a while to get to where it had those little tufts visible and then like three days later the thing was super thick.


--------------------
LAGM2020


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