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Invisibleherrenvolk


Registered: 05/15/16
Posts: 222
Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24759186 - 11/04/17 02:25 PM (4 years, 1 month ago)

Quote:

Josex said:
Now compare that with poking a culture with a needle, which is done very fast and cleanly, and using just a bunch of cells protected inside the needle to inoculate.
It is orders of magnitude safer, it's obvious, isn't it? No real skill is required to do that procedure safely either, anyone can do it, so you no longer can call LC an "advanced" technique if you do it this way.





Those are some good points you make, I'll definitely try this then. All I have are 16ga needles, which are rather large, is that alright? What gauge needles do you use?


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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: herrenvolk]
    #24759199 - 11/04/17 02:37 PM (4 years, 1 month ago)

Go to the section 'Prepping the syringe' in the OP, the info you need is there.


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InvisibleJust_A_Noob
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24760259 - 11/04/17 11:31 PM (4 years, 1 month ago)

I'll be trying this tonight  :cheers:


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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: Just_A_Noob]
    #24760274 - 11/04/17 11:42 PM (4 years, 1 month ago)

:bender:


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Invisiblemicelio
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24763571 - 11/06/17 12:26 PM (4 years, 30 days ago)

In four days both jars (turkey tail and B+), are loaded with beautiful cultures with the Josex Poke method....

This is a great way to eliminate contaminates in your Lc's

I had some doubts at first.... But not now....!

Thank You..  Josex....


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InvisiblebodhisattaMDiscordReddit
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Re: The Biopsy Method: A different way of doing LC. [Re: micelio]
    #24763593 - 11/06/17 12:38 PM (4 years, 30 days ago)

I should go look at mine.


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Invisiblevan hattonFacebookDiscord
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Re: The Biopsy Method: A different way of doing LC. [Re: bodhisatta]
    #24763708 - 11/06/17 01:47 PM (4 years, 29 days ago)

4 days I used the method with some brf plates (josex idea) nice white growth coming in. :thumbup:


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Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

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InvisiblemushboyMDiscord
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Re: The Biopsy Method: A different way of doing LC. [Re: van hatton]
    #24763810 - 11/06/17 02:39 PM (4 years, 29 days ago)

made some ape broth with the poke:naughty:


using the blunt tipped needle is more of 'core' sample taking. both ways work. i just happen to have spare blunt tips:shrug:


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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: mushboy]
    #24763889 - 11/06/17 03:12 PM (4 years, 29 days ago)

Quote:

micelio said:
In four days both jars (turkey tail and B+), are loaded with beautiful cultures with the Josex Poke method....

This is a great way to eliminate contaminates in your Lc's

I had some doubts at first.... But not now....!

Thank You..  Josex....



Awesome man, looking forward to seeing your results.
Quote:

van hatton said:
4 days I used the method with some brf plates (josex idea) nice white growth coming in. :thumbup:



Do you mean pf tek pucks? That's gonna get you a nice clean culture but if you want bad ass growth try cvg+brf pucks, good to test clones/cultures or snatch some clones when they grow out, it also has some 'cleaning effect' if a culture does not look that pretty for whatever reason.
Quote:

mushboy said:
made some ape broth with the poke:naughty:


using the blunt tipped needle is more of 'core' sample taking. both ways work. i just happen to have spare blunt tips:shrug:




Noice! Dude I wish I had petris just to take those kinda pics, have some biopsy action on agar right now, trying to get some isolates out of some clones (useless I know) just for the fuck of it.
That's some wide ass needle you got there. :lol:
I find it strange you can actually take a 'plug', coz I tried with a 14g blunt needle and could not see anything in it.
When you poke some myc fragments will rub off on the needle but it can't be seen. I compare it with putting your finger on a glass, you aren't going to see anything but lots of skin cells will be there nonetheless.


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InvisiblemushboyMDiscord
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24763899 - 11/06/17 03:19 PM (4 years, 29 days ago)

60mm dishes. perfect size:thumbup:

Quote:

That's some wide ass needle you got there



:smuglook: thats what they tell me

maybe im not letting the needle cool long enough?so the core sticks inside? in fact, i barely cool. i stab shit while hot.


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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: mushboy]
    #24763933 - 11/06/17 03:35 PM (4 years, 29 days ago)

Curious, when I started doing this I slso thought the needle had to be kinda warm for it to work, enough without killing the myc, but a cold needle will work the same.


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Invisiblevan hattonFacebookDiscord
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24764373 - 11/06/17 06:24 PM (4 years, 29 days ago)

:takingnotes: I'll throw some cvg in next time as well.

One culture I'm trying to clean up the second one is for fun.

Going to try this with lc when these plates grow out (if they dont comtam). :cheers:


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


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InvisibleTheMadHatter420
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Re: The Biopsy Method: A different way of doing LC. [Re: van hatton]
    #24764406 - 11/06/17 06:39 PM (4 years, 29 days ago)

I got 5 jars, 400ml each of some diluted grain water with a pinch of dextrose for the fuck of it. Got some clean LGT clone plates as well. Tomorrow I will inoculate some LGT and Blue Oyster LC jars.


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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: van hatton]
    #24764835 - 11/06/17 09:20 PM (4 years, 29 days ago)

Quote:

van hatton said:
:takingnotes: I'll throw some cvg in next time as well.

One culture I'm trying to clean up the second one is for fun.

Going to try this with lc when these plates grow out (if they dont comtam). :cheers:




Never use pucks to inoculate your LC, let alone the one you're using to clean up your stuff. You won't be able to inspect the culture easily with these and they can hide nasties. If you think the pucks are clean go ahead and poke them to transfer to agar.

Good luck Madhatter :super:


Edited by Josex (11/06/17 09:28 PM)


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Invisiblevan hattonFacebookDiscord
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24764932 - 11/06/17 10:10 PM (4 years, 29 days ago)

:lol:

Different plates would be used for the lc. Should of specified.


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: van hatton]
    #24765026 - 11/06/17 11:00 PM (4 years, 29 days ago)

That's a weight off my back :lol:
I'm rooting for you Van :cool:


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Invisibleinsanemike
Registered: 02/24/14
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24765654 - 11/07/17 09:07 AM (4 years, 29 days ago)

Can inside stipe tissue harbor bacteria? If not, I can see how using a flame sterilized blade to cut the butt off the stipe and using a flamed sterilized needle to collect inside tissue to noc an LC directly without going to agar first. I know it is not common practice but it could work well if the inner tissue isn't compromised in anyway.


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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: insanemike]
    #24765899 - 11/07/17 11:02 AM (4 years, 29 days ago)

I don't know, maybe. I always shoot the tissue on agar first just to make sure. It's a tradeoff between waiting some days to test it on agar and shaving off some days but without any guarantee the LC will be clean.


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InvisibleComebackKid
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Re: The Biopsy Method: A different way of doing LC. [Re: insanemike]
    #24766848 - 11/07/17 05:36 PM (4 years, 28 days ago)

Quote:

insanemike said:
Can inside stipe tissue harbor bacteria? If not, I can see how using a flame sterilized blade to cut the butt off the stipe and using a flamed sterilized needle to collect inside tissue to noc an LC directly without going to agar first. I know it is not common practice but it could work well if the inner tissue isn't compromised in anyway.




Cutting the stipe with a flame sterilized blade is still bad practice. Even thought the blade is sterile it can still push bacteria from the surface of the fruit to the inside.
You will have much higher success ripping the stipe


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Invisibleinsanemike
Registered: 02/24/14
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Re: The Biopsy Method: A different way of doing LC. [Re: ComebackKid]
    #24766895 - 11/07/17 05:52 PM (4 years, 28 days ago)

How do you rip the stipe? I'm a little confused? You do not use a blade to pierce into the stipe?


Edited by insanemike (11/07/17 05:53 PM)


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