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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: Snazz]
    #24757077 - 11/03/17 03:53 PM (3 years, 10 months ago)

Nice Bod! :rockon: Da faq is beer wort LC? got any pics?

Quote:

Snazz said:
What kind of myc density are we looking for to use an LC as an inoculant ?

Presume it scales on the amount of grain used.  Mycobag, quart, pint.

Thxx

Day 6 of two LCs PE and Pan Cyan

Pan has really taken off. Started with a dime sized transfer.

PE was a T3 half tiger drop which has gone pretty crazy in the past couple days.




Dude I like your enthusiasm but what you're doing is not the tek at all :rofl:


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Clean yo shit
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OfflineSnazz
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24757087 - 11/03/17 03:57 PM (3 years, 10 months ago)

Hah, ya. I did do a similar transfer size last week too.  Just figured you have some experience using LC, whereas I have none.  Kinda avoided it !


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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24757106 - 11/03/17 04:04 PM (3 years, 10 months ago)

Quote:

Josex said:
Nice Bod! :rockon: Da faq is beer wort LC? got any pics?

Quote:

Snazz said:
What kind of myc density are we looking for to use an LC as an inoculant ?

Presume it scales on the amount of grain used.  Mycobag, quart, pint.

Thxx

Day 6 of two LCs PE and Pan Cyan

Pan has really taken off. Started with a dime sized transfer.

PE was a T3 half tiger drop which has gone pretty crazy in the past couple days.




Dude I like your enthusiasm but what you're doing is not the tek at all :rofl:



Ill take some. Wort is basically grain soak water. But the grains are modified so the starches are converted to sugars.

Wort gets boiled and hops are added to the boil. The boiled wort is cooled and inoculated with yeast.

So wort is sugar malted grain water.

Grain proteins make their way into the water and when it gets boiled they break down into free amino nitrogen.


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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: Snazz] * 1
    #24757115 - 11/03/17 04:07 PM (3 years, 10 months ago)

Snazz@ Just let the LC colonize and use it. You don't have to wait till 100% if you're in a rush, an LC at 80% is perfectly usable.


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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: bodhisatta]
    #24757117 - 11/03/17 04:10 PM (3 years, 10 months ago)

Don't know what you're talking about but it sounds fucking wonderful :popcorn:


--------------------
LC's are easy
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InvisiblebodhisattaMDiscordReddit
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24757131 - 11/03/17 04:13 PM (3 years, 10 months ago)

Well it is more sugary then starchy but it works well diluted to 2% or less sugar.


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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24757142 - 11/03/17 04:18 PM (3 years, 10 months ago)

Quote:

Josex said:
Snazz@ Just let the LC colonize and use it. You don't have to wait till 100% if you're in a rush, an LC at 80% is perfectly usable.




Thx! My P. Cyan var Hawaii might be ready this weekend then!


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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: Snazz]
    #24757175 - 11/03/17 04:39 PM (3 years, 10 months ago)

What have you got in the LC's Bod? A clone? MS?


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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24757241 - 11/03/17 05:17 PM (3 years, 10 months ago)

Neato, I'm gonna give it a poke.


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InvisiblebodhisattaMDiscordReddit
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex] * 1
    #24757244 - 11/03/17 05:18 PM (3 years, 10 months ago)

Quote:

Josex said:
What have you got in the LC's Bod? A clone? MS?



Pesa MS, pe6 MS, and something else i forgot


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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24757754 - 11/03/17 09:41 PM (3 years, 10 months ago)

Quote:

Josex said:
Don't know what you're talking about but it sounds fucking wonderful :popcorn:




Wort is like a soup broth or tea but made from grain. Think of wort as nonfermented beer, once yeast is added to it, it essentially is transformed into beer.

FYI-you can buy pre-made wort at your local brew shop.


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Re: The Biopsy Method: A different way of doing LC. [Re: bodhisatta]
    #24757856 - 11/03/17 10:35 PM (3 years, 10 months ago)

Quote:

bodhisatta said:
Wort gets boiled and hops are added to the boil. The boiled wort is cooled and inoculated with yeast.

So wort is sugar malted grain water.




Interesting.  I would have expected the antifungal properties of the hops to discourage mycelium growth on LC/agar made with wort.  I suppose yeast survives just fine in the presence of hops though, so perhaps cubensis mycelium are similarly immune. Perhaps using wort makes for a kind of antifungal, antibacterial grain water LC and/or agar.  I'm curious to know how well it works out.


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InvisiblebodhisattaMDiscordReddit
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Re: The Biopsy Method: A different way of doing LC. [Re: RatThing]
    #24757864 - 11/03/17 10:38 PM (3 years, 10 months ago)

There's no hops in the wort its preboil wort.

Hops don't have anti fungal properties though too many hops is harsh on yeast.
they have some antibacterial properties. Hop bitter acids act as ionophores


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InvisibleRatThing
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Re: The Biopsy Method: A different way of doing LC. [Re: bodhisatta]
    #24757872 - 11/03/17 10:43 PM (3 years, 10 months ago)

Quote:

bodhisatta said:
There's no hops in the wort its preboil wort.




Ahh ok.  That does seem to make more sense.


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Re: The Biopsy Method: A different way of doing LC. [Re: RatThing]
    #24758058 - 11/04/17 12:31 AM (3 years, 10 months ago)

Josex,
I made up 150mL of the mea recipe last sunday and did the poke. :syringe: I see the original poke (now fuzzy), plus visibile mycellium floating in the water, but it's not nearly as thick as the photo you have in the original photo. How do you know when it's ready?


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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: senseit]
    #24758246 - 11/04/17 03:20 AM (3 years, 10 months ago)

It will thicken up with time. It's ready when the myc colonizes most of the liquid, you'll know it :thumbup:


--------------------
LC's are easy
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24758342 - 11/04/17 05:24 AM (3 years, 10 months ago)

This seems interesting, I'll probably try it one of these days.

So does the "guarantees clean as fuck inoculants" part mainly come from using the really small piece of tissue to inoculate? I never really thought of that as a way to make cleaner transfers, I always thought if the plate was clean (or dirty), then it wouldn't really matter how large or small the transfer piece was. I'd be interested to hear your thoughts on the rationale behind this process. :thumbup:


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Re: The Biopsy Method: A different way of doing LC. [Re: herrenvolk]
    #24758445 - 11/04/17 07:50 AM (3 years, 10 months ago)

Taking small transfers away from the dirty part is how you get it clean. Your not going to just put spores to agar and have a clean plate.


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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: herrenvolk]
    #24758573 - 11/04/17 09:48 AM (3 years, 10 months ago)

Quote:

herrenvolk said:
This seems interesting, I'll probably try it one of these days.

So does the "guarantees clean as fuck inoculants" part mainly come from using the really small piece of tissue to inoculate? I never really thought of that as a way to make cleaner transfers, I always thought if the plate was clean (or dirty), then it wouldn't really matter how large or small the transfer piece was. I'd be interested to hear your thoughts on the rationale behind this process. :thumbup:




Inoculating with a small piece of tissue does not guarantee clean inoculants, you still need a clean culture or your LC will contaminate. What this method does for you is making the procedure of inoculation so simple and safe that it can no longer be considered a real vector for contamination for people who use SAB's. So it will only 'guarantee' fucking clean as fuck inoculants (fucking fuck) if the culture was clean.

You seem to be overlooking the part that sterile technique plays in making a clean LC using the standard way of inoculation, that is, agar wedge to LC, and why it's so important to test the LC if you inoculated it with a wedge.

So many things can go south even if you have mad skills with the scalpel. You need to cut the wedge and expose it to the air of the SAB and then drop the thing in the liquid more or less graciously, hoping the wedge won't get stuck in the blade... Now anxiously wait till the LC is ready in order to test it.

Now compare that with poking a culture with a needle, which is done very fast and cleanly, and using just a bunch of cells protected inside the needle to inoculate.
It is orders of magnitude safer, it's obvious, isn't it? No real skill is required to do that procedure safely either, anyone can do it, so you no longer can call LC an "advanced" technique if you do it this way.

Another perk of doing it this way is that you're going to narrow down the genetics like there's no tomorrow. That can be good most of the times if you work with MS, although it can also be bad in some cases, or have no repercussion at all if you work with isolates or clones.


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
LAGM '21

PHENO HUNTERS


Edited by Josex (11/04/17 10:08 AM)


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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24759107 - 11/04/17 01:47 PM (3 years, 10 months ago)

this clone is takin its sweet ass time pinning.



im never spawning while tripping again:facepalm:

and i spawned these the other day:derfase:


not happy with this clone but fukkit. its the other aspects that make this grow awesome:awesomenod:


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