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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: mushboy]
    #24849775 - 12/16/17 02:16 PM (3 years, 11 months ago)

IMO, a sterile inoculation port is the way to go, like the one I explain in the OP. You do that and you can be sure the only potential vector is the agar culture itself.
There's little risk in cracking a metal lid to inoculate but still, the risk is there. I don't mind a lil' extra peace of mind and turning LC's into child's play. :hehehe:

Imma post a trick soon for making a simple inoculation port for people who don't have SFD's.


Edited by Josex (12/16/17 04:02 PM)


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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24849799 - 12/16/17 02:25 PM (3 years, 11 months ago)

:rockon:


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Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


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Re: The Biopsy Method: A different way of doing LC. [Re: van hatton]
    #24850266 - 12/16/17 06:55 PM (3 years, 11 months ago)

All I use is plastic lids and I much prefer them. metal for me has many downfalls and side by side with plastic on no pour agar and my contam rate is double with metal.

I think the problem I can't handle metal lids without having my finger extend of the underside barely and that is causing the contam.

Also I've already made a few lc broths and a few biopsy inoculated broths with plastic lids and I have very clean fast colonizing myc. No cloudiness.

Already nocced up some cakes and thay colonized in 5 days.

I doubt that the difference of an lc jar and the agar no pour jar could cause any difference in contam but if there's a tiny bit of bacteria present it isn't going to bother me and my method.

I work in front of a hood so maybe there is a difference there as well. And I  often use hundereds of these plastic unmodded lids in a week so maybe I'm just really used to them.

So If I'm confident with my technique with plastic lids would you say they are good to use unmodded and screwed on tight (in other words no GE)?

I would only have half the jar filled with lc, so would the airspace above be enough for colonization withouth GE?


--------------------


Edited by tombosley8 (12/16/17 07:16 PM)


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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: tombosley8]
    #24851473 - 12/17/17 12:47 PM (3 years, 11 months ago)

Quote:

So If I'm confident with my technique with plastic lids would you say they are good to use unmodded and screwed on tight (in other words no GE)?




Sure, you can. My LC's have so little GE that is negligible and they grow fine and fast. I just use SFD's because of how I like to inoculate through the makeshift port. CBK and FM have done several LC's with no GE at all and Violet has been doing the same for ages. I don't notice any difference when I don't add the port and therefore the LC has much more GE.


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Re: The Biopsy Method: A different way of doing LC. [Re: Josex] * 1
    #24851579 - 12/17/17 01:40 PM (3 years, 11 months ago)

some pe6


i need i better backlight:tongue:

cant wait for these bitches to pin.
texas g biopsy lc 2nd growing doing the poke:thumbup:


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Re: The Biopsy Method: A different way of doing LC. [Re: mushboy]
    #24866697 - 12/24/17 10:54 PM (3 years, 11 months ago)

i think josex mentioned how week LCs are lazy and just lay near the bottom of the broth.

1g lme. floats nicely in the broth. this has only been swirled a few times.


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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: mushboy]
    #24866710 - 12/24/17 11:00 PM (3 years, 11 months ago)

Tammed LC's are lazy :lol:  Yours looks clean as hell. I'm seeing a lot of good lc pics here, where you really can distinguish if it's clean or not, that's rare. I can't take a good pic of an LC to save my life.


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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24868075 - 12/25/17 06:49 PM (3 years, 11 months ago)

How does this look



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Re: The Biopsy Method: A different way of doing LC. [Re: JHOVA]
    #24868087 - 12/25/17 07:00 PM (3 years, 11 months ago)

What recipe did you use J?


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InvisiblemushboyMDiscord
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Re: The Biopsy Method: A different way of doing LC. [Re: Mateja]
    #24868090 - 12/25/17 07:06 PM (3 years, 11 months ago)

Looks like brf.

I could only see the myc after like 2weeks. Brf broth is nasty like that:doggystyle:


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Re: The Biopsy Method: A different way of doing LC. [Re: mushboy]
    #24868112 - 12/25/17 07:32 PM (3 years, 11 months ago)

2 g brf. I inoculated i think 12/19.


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Re: The Biopsy Method: A different way of doing LC. [Re: JHOVA]
    #24878232 - 12/30/17 10:36 AM (3 years, 10 months ago)

Started a few lc's from your method josex. One ape and one pe.

This is the ape plate



That went into LME ..75 g to 250 ml on 12/16


This is the PE LC. Don't have a pic of plate, but same recipe and date.


Ape LC went into 14 quarts of wbs today, 12/30
PE LC is going into 14 quarts of wbs tomorrow

Thanks again josex, great method.
And thanks to all for your input and ideas as well.
:thaaannks:

Edit:

Question for Mush,

I know you are a fellow fan of the wbs soak method. I was wondering if you change the loading damp step, since you will be pouring in more liquid in form of the LC. I just ended up letting mine seed dry a bit longer than normal.
Was just wondering since you are having some great results with your lc's. Thanks mang


Edited by Pipefitter537 (12/30/17 10:47 AM)


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Re: The Biopsy Method: A different way of doing LC. [Re: Pipefitter537]
    #24878248 - 12/30/17 10:51 AM (3 years, 10 months ago)

Nope! Even when loading damp,  with the soak the grains are still sliiightly dry.

Perfect for large doses of broth.

Thats some sexy lc!  I just poured my pe6 myself:cuteshit:


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Re: The Biopsy Method: A different way of doing LC. [Re: mushboy]
    #24878275 - 12/30/17 11:04 AM (3 years, 10 months ago)

Cool. I will try that today. I have a batch soaking right meow. I can compare if the two batches differ.
Thanks buddy.


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Re: The Biopsy Method: A different way of doing LC. [Re: Pipefitter537]
    #24878375 - 12/30/17 11:56 AM (3 years, 10 months ago)

This method is so stupid reliable.

I've lost count of how many LCS I actually have.

I actually am going to open a lc in open air just so I know what a bad lc looks like. Tf?

Still blown away by this and the other methods you posted. Kick ass :rockon:


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


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Re: The Biopsy Method: A different way of doing LC. [Re: van hatton]
    #24878494 - 12/30/17 01:01 PM (3 years, 10 months ago)

ive made like 30:winning1:

various strengths and shit. only one contamed. this first one i made. but josex even said the culture looked off:shrug:

lessons learned. thanks for the brain food.


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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: van hatton]
    #24878840 - 12/30/17 03:41 PM (3 years, 10 months ago)

Nice Pipefitter537 :rockon:

Quote:


van hatton said:
This method is so stupid reliable.

I've lost count of how many LCS I actually have.

I actually am going to open a lc in open air just so I know what a bad lc looks like. Tf?

Still blown away by this and the other methods you posted. Kick ass :rockon:




Dude as I told you in my pm's, I'm especially happy for you, after all you've been trough.

It should be worth noting that Van did all his LC's without using agar, so the old premise "the only way to inoculate LC's safely is using agar" is obviously false. I've done it myself a bunch of times too. He used a mix of coir and brf to make pucks like these that were inoculated with biopsies.


This should be very interesting for people who for whatever reason can't get agar to work for them. Like a complete beginner who's afraid of getting into agar, people who don't own a PC (yeah you can make clean LC's without a PC and without agar :shrug:) and people with any sort of handicap that makes it impossible for them to do agar work or for people with more experience that have a huge spore load in their house or simply can't seem to get rid of bacteria in an agar culture. All it takes is cracking a lid and squirting some water from a syringe to inoculate the puck, virtually impossible to fail.

There's a caveat, though. I don't know if I can recommend transferring from puck to puck by poking alone. Using the poke on the same culture more than two times may have its drawbacks. The cellular division that needs to take place for a microscopic piece of tissue to form a colony from scratch should be taken into account.
Taking samples using a scalpel from coir+brf pucks is easy, you just have to dig the scalpel in the sub to scoop up a tiny piece and transfer it to another puck, unlike brf pucks that can be a little difficult to take a sample from using the scalpel.


Edited by Josex (12/31/17 02:01 AM)


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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24878868 - 12/30/17 04:00 PM (3 years, 10 months ago)

Thsts whats up man! Yay van.


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Re: The Biopsy Method: A different way of doing LC. [Re: mushboy]
    #24878962 - 12/30/17 04:56 PM (3 years, 10 months ago)

:rockon:

I've seriously had mold on one of those brf coir plates. It was because I questioned the culture and open it and poked it with my finger :lol:

Still took 9~ days to germ. The other 2 plates I opened that day never molded.

Bacteria doesn't even exist anymore :lol:

One thing that's nice to me is that you can is one puck for as many times as you can poke it. So you minimize expansion vs doing an additional transfer for more too work with.

Sorry if that doesn't make sense I'm sick as fuck


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


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Re: The Biopsy Method: A different way of doing LC. [Re: van hatton]
    #24879087 - 12/30/17 06:08 PM (3 years, 10 months ago)

Hey van i asked you a while ago if you could make a summary of your experiences in achieving clean spawn. Have you thought about putting sometjing together? Im sure there are others that could benefit from your diligent work.


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